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The map label for this gene is hisF [H]

Identifier: 87199032

GI number: 87199032

Start: 1051962

End: 1052735

Strand: Direct

Name: hisF [H]

Synonym: Saro_1010

Alternate gene names: 87199032

Gene position: 1051962-1052735 (Clockwise)

Preceding gene: 87199031

Following gene: 87199033

Centisome position: 29.54

GC content: 68.09

Gene sequence:

>774_bases
ATGACTGTCCGCGTCCGCGTCATTCCCTGTCTCGACGTTGCCGATGGCCGCGTGGTCAAGGGCGTCAATTTCGTCGACCT
GCGCGATGCGGGAGATCCGGTCGAGCAGGCGCGCGCCTATGACAAGGCTGGCGCCGACGAGCTTTGCTTCCTCGACATTT
CCGCAAGCCACGAAGGCCGCGGCACCCTGCTCGATGTCGTCGCCCGCACCGCAGAAGTCTGCTTCATGCCGCTGACCGTC
GGCGGCGGGGTGAGGGCGGTGGAAGATGCCCGCGCGCTCCTGCTGGCGGGAGCTGACAAGGTCGCGGTCAATTCCGCCGC
CGTCGCCCGGCCCGAACTGGTGCGCGACATTGCCGAGAAGTTCGGCGCTCAGTGCGTTGTCGGTTCGGTCGACGCGCGCC
GCGTCAGCGAACCCGGCGCTCCGGGCAGATGGGAAATCTTCACCCATGGCGGACGCAAGGCGACGGGAATCGACGCTCTG
GCGCACGCCGTGAACCTTGCCACGCTGGGTGCGGGCGAACTCCTCGTGACCTCGATGGACGGCGACGGTACGCAGAAGGG
CTATGATCTCGAACTGACCCGCGCGATTGCCGATGCCGTGCCGGTTCCGGTGATCGCCAGCGGCGGCGTCGGCTCGCTGG
AACATCTGGTGGAAGGGGTGACCAAGGGTCACGCCAGCGCGGTTCTCGCAGCCTCCATCTTCCACTTCGGGACCTACACC
ATCGCCGAGGCGCACGCGGCGCTGCGTGCGGCGGGCCTGCCAGCGCGCGGCTGA

Upstream 100 bases:

>100_bases
CACGAGGGCGACGGAATTGAAGGTGTCATCACCGGCCGGGCGCTCTATGATGGACGTCTCGATCTGAAGGCCGCGATCGA
GATGGCAGAGGCCTGATTCC

Downstream 100 bases:

>100_bases
AATGCGCCGTGTCCCGCAATGGGGCATTAACCAACTGATATAAGGGATTTTACCGAATCACCCGATCTGGCAGGGCTAGG
CCGTAAACCAATAGCACAGG

Product: imidazole glycerol phosphate synthase subunit HisF

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MTVRVRVIPCLDVADGRVVKGVNFVDLRDAGDPVEQARAYDKAGADELCFLDISASHEGRGTLLDVVARTAEVCFMPLTV
GGGVRAVEDARALLLAGADKVAVNSAAVARPELVRDIAEKFGAQCVVGSVDARRVSEPGAPGRWEIFTHGGRKATGIDAL
AHAVNLATLGAGELLVTSMDGDGTQKGYDLELTRAIADAVPVPVIASGGVGSLEHLVEGVTKGHASAVLAASIFHFGTYT
IAEAHAALRAAGLPARG

Sequences:

>Translated_257_residues
MTVRVRVIPCLDVADGRVVKGVNFVDLRDAGDPVEQARAYDKAGADELCFLDISASHEGRGTLLDVVARTAEVCFMPLTV
GGGVRAVEDARALLLAGADKVAVNSAAVARPELVRDIAEKFGAQCVVGSVDARRVSEPGAPGRWEIFTHGGRKATGIDAL
AHAVNLATLGAGELLVTSMDGDGTQKGYDLELTRAIADAVPVPVIASGGVGSLEHLVEGVTKGHASAVLAASIFHFGTYT
IAEAHAALRAAGLPARG
>Mature_256_residues
TVRVRVIPCLDVADGRVVKGVNFVDLRDAGDPVEQARAYDKAGADELCFLDISASHEGRGTLLDVVARTAEVCFMPLTVG
GGVRAVEDARALLLAGADKVAVNSAAVARPELVRDIAEKFGAQCVVGSVDARRVSEPGAPGRWEIFTHGGRKATGIDALA
HAVNLATLGAGELLVTSMDGDGTQKGYDLELTRAIADAVPVPVIASGGVGSLEHLVEGVTKGHASAVLAASIFHFGTYTI
AEAHAALRAAGLPARG

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=255, Percent_Identity=40, Blast_Score=187, Evalue=7e-49,
Organism=Escherichia coli, GI87082028, Length=249, Percent_Identity=28.1124497991968, Blast_Score=85, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6319725, Length=310, Percent_Identity=33.2258064516129, Blast_Score=141, Evalue=8e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.- [C]

Molecular weight: Translated: 26425; Mature: 26294

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRVRVIPCLDVADGRVVKGVNFVDLRDAGDPVEQARAYDKAGADELCFLDISASHEGR
CEEEEEEEEEEECCCCCEECCCCEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCC
GTLLDVVARTAEVCFMPLTVGGGVRAVEDARALLLAGADKVAVNSAAVARPELVRDIAEK
CHHHHHHHHHHHHEEEEEEECCCCCHHHHHHEEEEECCCHHEECCHHHCCHHHHHHHHHH
FGAQCVVGSVDARRVSEPGAPGRWEIFTHGGRKATGIDALAHAVNLATLGAGELLVTSMD
HCCEEEEECCCHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHEECCCCEEEEECC
GDGTQKGYDLELTRAIADAVPVPVIASGGVGSLEHLVEGVTKGHASAVLAASIFHFGTYT
CCCCCCCCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
IAEAHAALRAAGLPARG
HHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TVRVRVIPCLDVADGRVVKGVNFVDLRDAGDPVEQARAYDKAGADELCFLDISASHEGR
EEEEEEEEEEECCCCCEECCCCEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCC
GTLLDVVARTAEVCFMPLTVGGGVRAVEDARALLLAGADKVAVNSAAVARPELVRDIAEK
CHHHHHHHHHHHHEEEEEEECCCCCHHHHHHEEEEECCCHHEECCHHHCCHHHHHHHHHH
FGAQCVVGSVDARRVSEPGAPGRWEIFTHGGRKATGIDALAHAVNLATLGAGELLVTSMD
HCCEEEEECCCHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHEECCCCEEEEECC
GDGTQKGYDLELTRAIADAVPVPVIASGGVGSLEHLVEGVTKGHASAVLAASIFHFGTYT
CCCCCCCCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
IAEAHAALRAAGLPARG
HHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA