Click here to switch to the map view.

The map label for this gene is hisH

Identifier: 87199029

GI number: 87199029

Start: 1049375

End: 1050004

Strand: Direct

Name: hisH

Synonym: Saro_1007

Alternate gene names: 87199029

Gene position: 1049375-1050004 (Clockwise)

Preceding gene: 87199028

Following gene: 87199030

Centisome position: 29.46

GC content: 67.62

Gene sequence:

>630_bases
GTGGCTGAGACGCTCGCGCTCGTCGACTATGGCGCGGGCAACCTGCGCTCGGTTGCAAATGCGCTCAAGGCGGCGGGGGC
CGAGGGCGTCGTGGTGACCGCCGATCCTGCGGTCGTTCGGGCCGCAGATCGCGTCGTTCTGCCCGGCGTAGGTGCCTTCA
AGGCCTGCATAGGTGCTCTCAGGGGCGTTTCGGGTCTTGTCGAGGCGATGGAGGAGCGGGTGCTCGTGGGCGGTGCTCCG
TTCCTCGGCATTTGCGTGGGAATGCAGCTCCTTGCCGACCGCGGCGTGGAACACGGCGTCACCGAGGGCCTCGGCTGGAT
CGGCGGAGAAGTCCGCGTGATCGAGCCGGCCGATCCGTCGATCAAGGTGCCCCACATGGGCTGGAACGACGTCGCGCCGA
TGCCCCACGAAGGCGGCGCGGAACTGATCGAGCCCGGCGAGGCCTATTTCCTGCACTCGTACCACTTCGTCACCGACGCC
GGCGCGCACATCGCGGCGATGAGCGACCACGGTGGCGGGATCGTCGCGGCGGTCGCGCGCGACAATATCCTGGGCGTGCA
GTTCCACCCGGAAAAGAGCCAGTCCTACGGCCTTTCTTTGCTCGCCCGCTTCCTCGAATGGAAGCCCTGA

Upstream 100 bases:

>100_bases
ATCGAGGGCATCTACAAGGGCTTCGCCCGTGCGATGCGCGCCGCCATCGCGATCGATCCGCGCAAGGCCGACGCCGTCCC
TTCGACCAAGGGCATCCTCG

Downstream 100 bases:

>100_bases
CGCGGCAGCCTGCGGGAACCTGACCGAGGCGCGGGGGCTTCCTACCTTTGAAGGAGGCCCCGCCAATGACCCAGTTCCCG
TTCGACCGCCTGGTCTTCAC

Product: imidazole glycerol phosphate synthase subunit HisH

Products: AMP; diphosphate; GMP; L-glutamate

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MAETLALVDYGAGNLRSVANALKAAGAEGVVVTADPAVVRAADRVVLPGVGAFKACIGALRGVSGLVEAMEERVLVGGAP
FLGICVGMQLLADRGVEHGVTEGLGWIGGEVRVIEPADPSIKVPHMGWNDVAPMPHEGGAELIEPGEAYFLHSYHFVTDA
GAHIAAMSDHGGGIVAAVARDNILGVQFHPEKSQSYGLSLLARFLEWKP

Sequences:

>Translated_209_residues
MAETLALVDYGAGNLRSVANALKAAGAEGVVVTADPAVVRAADRVVLPGVGAFKACIGALRGVSGLVEAMEERVLVGGAP
FLGICVGMQLLADRGVEHGVTEGLGWIGGEVRVIEPADPSIKVPHMGWNDVAPMPHEGGAELIEPGEAYFLHSYHFVTDA
GAHIAAMSDHGGGIVAAVARDNILGVQFHPEKSQSYGLSLLARFLEWKP
>Mature_208_residues
AETLALVDYGAGNLRSVANALKAAGAEGVVVTADPAVVRAADRVVLPGVGAFKACIGALRGVSGLVEAMEERVLVGGAPF
LGICVGMQLLADRGVEHGVTEGLGWIGGEVRVIEPADPSIKVPHMGWNDVAPMPHEGGAELIEPGEAYFLHSYHFVTDAG
AHIAAMSDHGGGIVAAVARDNILGVQFHPEKSQSYGLSLLARFLEWKP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=205, Percent_Identity=37.5609756097561, Blast_Score=114, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6319725, Length=213, Percent_Identity=30.5164319248826, Blast_Score=92, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 6.3.5.2

Molecular weight: Translated: 21666; Mature: 21535

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETLALVDYGAGNLRSVANALKAAGAEGVVVTADPAVVRAADRVVLPGVGAFKACIGAL
CCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCHHHHHCCEEEECCCHHHHHHHHHH
RGVSGLVEAMEERVLVGGAPFLGICVGMQLLADRGVEHGVTEGLGWIGGEVRVIEPADPS
HHHHHHHHHHHHCEEECCCCHHHHHHHHHHHHHCCCCCCHHHCHHHCCCEEEEECCCCCC
IKVPHMGWNDVAPMPHEGGAELIEPGEAYFLHSYHFVTDAGAHIAAMSDHGGGIVAAVAR
EECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCEEEEEECCCCCEEEEEEC
DNILGVQFHPEKSQSYGLSLLARFLEWKP
CCEEEEEECCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AETLALVDYGAGNLRSVANALKAAGAEGVVVTADPAVVRAADRVVLPGVGAFKACIGAL
CCEEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCHHHHHCCEEEECCCHHHHHHHHHH
RGVSGLVEAMEERVLVGGAPFLGICVGMQLLADRGVEHGVTEGLGWIGGEVRVIEPADPS
HHHHHHHHHHHHCEEECCCCHHHHHHHHHHHHHCCCCCCHHHCHHHCCCEEEEECCCCCC
IKVPHMGWNDVAPMPHEGGAELIEPGEAYFLHSYHFVTDAGAHIAAMSDHGGGIVAAVAR
EECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCEEEEEECCCCCEEEEEEC
DNILGVQFHPEKSQSYGLSLLARFLEWKP
CCEEEEEECCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; xanthosine 5'-phosphate; L-glutamine; H2O

Specific reaction: ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA