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The map label for this gene is lieA [H]

Identifier: 87198866

GI number: 87198866

Start: 893966

End: 895585

Strand: Direct

Name: lieA [H]

Synonym: Saro_0844

Alternate gene names: 87198866

Gene position: 893966-895585 (Clockwise)

Preceding gene: 87198865

Following gene: 87198867

Centisome position: 25.1

GC content: 65.43

Gene sequence:

>1620_bases
ATGACATCTTGCCTGCGCGGGGCCGCGAGCGCCGCCGCCATTGCCCTTTGCACGTTCGCCGCCCCGGCCATTGCCGGACC
CGCCGAGGACAAGCTGATCGCGCAGCTCCTGGACGAGGGATTGAACCGTTCCGATGCGATGGAGATCGCGTCGGAGCTGA
TGGACCGCATCGGGCCGCGTCTCACCAATTCCGAAAATCATCGCAAGGCGGAGGACTGGGCTGCAGCCAAGTTCGCCTCG
TTCGGGCTCAAGAACATCCACCGCGAACCGTTCGAGTTCGGGCTTGGCTGGAACCTCAAGTCCTATTCGGCGACGATGAC
CTCGCCGCGCAGCCTGCCCCTCACCGTCCTGCCGGTCGCATGGTCGCCGCCCACCGGCGGCACGATCACCGCCCCGGTCA
TCGTCGCGCCGATGACCAAGGTCGAGAATTTCGATGCGTGGAAGGGCAAGCTGGCCGGCCGGATCGTGCTGGTCAGCCTG
CCTGGCGAGACCAGCGCGTCCGCAGATCCGGTGTTCGAGCGCCTCTCGGGCGAAGAGATCGGCAAGCTCGACAAGTATAC
CTTGCCGCGCCACGACCCCGAAGGTCTGGCCATGCAGGTCGCCCGGCGTGGATTTGCCCGCAAGCTGTCGGAGTTCCTCA
AGGCGGAAGGCGCGTTGGCCATGGTCCGCATGACCTATCGCGATGGCAAGCTGGTCCATGGCGAGGGCTATGACTTCGTG
CCGGGCCAGACCCTTGCCGTGCCGGCGATGGACATGGCGCAGGAAGACTATCGCCGCCTCGTCCGCCTGGAGAAGACGGG
TGCCGCCCCGCAGCTCTCGCTCAGCATCGACGCGAGCTTCGACGACAAGGATCTGATGGCGGACAACGTCATTGCCGAGA
TCCCCGGCAGCGATCCCAAGGCGGGCTACGTCATGGCGGGTGCGCACTTCGACAGCTGGATCGCGGGCGACGGCGCATCC
GACAATGGAGCGGGAAGCGTCGCCGTGATCGAGGCTGCACGCCTGCTCTCGAAAATGGGCGTCAAGCCGAAGCGCACCAT
CCGCTTTGCGCTGTGGAGCGGGGAGGAGCAGGGGCTGCTGGGCTCGAAGGCCTATATCGAGCAGCATCTCGCCACCCGGC
CGGTCGACCCGGCGCTAAAGGGCATCGACAGCTATTCGGCATGGCGCAATGCCTATCCGATCACGCCCAAGCCGGGCTAT
TCCCAGCTCAAGGCCTATTTCAACATGGACAACGGTTCGGGCAAGTTCCGCGGCATTTATGCCGAGGGCAACGTTGCCGC
CGCGCCGATCCTCAGGGAATGGCTCGCGCCCTTCAGCTCGCTCGGCGCGGACAAGGTGGTGATGAGCAAGACGGGCGGGA
CCGACCACGTCTATCTCCAGGCAATCGGCCTGCCGGGCTACCAGTTCATCCAGGACCCGCTCGATTACGAGAGCCGCGTG
CACCACTCCAGCCTCGACACGCTTGACCACATGCGCGCCGACGACATGAGGCAGGCCTCCGTCATTCTGGCGGGAATGCT
GCTCCAGGCGGCGACAAGCGAGAAGGAACTGCCGCGCTCGCCGTTGCCGACCAAGCCGGACGCGACCGATCCGTTCAAGG
TGCAGGACCCCAACCAGTAG

Upstream 100 bases:

>100_bases
CGCCCTCAGGGGCGATGAATTTTCGATCAAAGGGCTTGGCTTTCCCCGCGGCGGCGGCAAGATGGTTTCCAGAGAAACCG
ACCCGGGGAGTTTTCCAGAC

Downstream 100 bases:

>100_bases
GCACTTGTCCCGGCGGAGGTGCGGCTGCGTTGCGAGCTGCCTTCCGCCGCCATAGGTGACCGTACGGATATTGCCATCGG
TACTTGTCTCAAGCGGCTTT

Product: peptidase M28

Products: NA

Alternate protein names: LIE1 [H]

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MTSCLRGAASAAAIALCTFAAPAIAGPAEDKLIAQLLDEGLNRSDAMEIASELMDRIGPRLTNSENHRKAEDWAAAKFAS
FGLKNIHREPFEFGLGWNLKSYSATMTSPRSLPLTVLPVAWSPPTGGTITAPVIVAPMTKVENFDAWKGKLAGRIVLVSL
PGETSASADPVFERLSGEEIGKLDKYTLPRHDPEGLAMQVARRGFARKLSEFLKAEGALAMVRMTYRDGKLVHGEGYDFV
PGQTLAVPAMDMAQEDYRRLVRLEKTGAAPQLSLSIDASFDDKDLMADNVIAEIPGSDPKAGYVMAGAHFDSWIAGDGAS
DNGAGSVAVIEAARLLSKMGVKPKRTIRFALWSGEEQGLLGSKAYIEQHLATRPVDPALKGIDSYSAWRNAYPITPKPGY
SQLKAYFNMDNGSGKFRGIYAEGNVAAAPILREWLAPFSSLGADKVVMSKTGGTDHVYLQAIGLPGYQFIQDPLDYESRV
HHSSLDTLDHMRADDMRQASVILAGMLLQAATSEKELPRSPLPTKPDATDPFKVQDPNQ

Sequences:

>Translated_539_residues
MTSCLRGAASAAAIALCTFAAPAIAGPAEDKLIAQLLDEGLNRSDAMEIASELMDRIGPRLTNSENHRKAEDWAAAKFAS
FGLKNIHREPFEFGLGWNLKSYSATMTSPRSLPLTVLPVAWSPPTGGTITAPVIVAPMTKVENFDAWKGKLAGRIVLVSL
PGETSASADPVFERLSGEEIGKLDKYTLPRHDPEGLAMQVARRGFARKLSEFLKAEGALAMVRMTYRDGKLVHGEGYDFV
PGQTLAVPAMDMAQEDYRRLVRLEKTGAAPQLSLSIDASFDDKDLMADNVIAEIPGSDPKAGYVMAGAHFDSWIAGDGAS
DNGAGSVAVIEAARLLSKMGVKPKRTIRFALWSGEEQGLLGSKAYIEQHLATRPVDPALKGIDSYSAWRNAYPITPKPGY
SQLKAYFNMDNGSGKFRGIYAEGNVAAAPILREWLAPFSSLGADKVVMSKTGGTDHVYLQAIGLPGYQFIQDPLDYESRV
HHSSLDTLDHMRADDMRQASVILAGMLLQAATSEKELPRSPLPTKPDATDPFKVQDPNQ
>Mature_538_residues
TSCLRGAASAAAIALCTFAAPAIAGPAEDKLIAQLLDEGLNRSDAMEIASELMDRIGPRLTNSENHRKAEDWAAAKFASF
GLKNIHREPFEFGLGWNLKSYSATMTSPRSLPLTVLPVAWSPPTGGTITAPVIVAPMTKVENFDAWKGKLAGRIVLVSLP
GETSASADPVFERLSGEEIGKLDKYTLPRHDPEGLAMQVARRGFARKLSEFLKAEGALAMVRMTYRDGKLVHGEGYDFVP
GQTLAVPAMDMAQEDYRRLVRLEKTGAAPQLSLSIDASFDDKDLMADNVIAEIPGSDPKAGYVMAGAHFDSWIAGDGASD
NGAGSVAVIEAARLLSKMGVKPKRTIRFALWSGEEQGLLGSKAYIEQHLATRPVDPALKGIDSYSAWRNAYPITPKPGYS
QLKAYFNMDNGSGKFRGIYAEGNVAAAPILREWLAPFSSLGADKVVMSKTGGTDHVYLQAIGLPGYQFIQDPLDYESRVH
HSSLDTLDHMRADDMRQASVILAGMLLQAATSEKELPRSPLPTKPDATDPFKVQDPNQ

Specific function: A leucine-specific metalloprotease that plays a role in controlling the amount of leupeptin during colony development. Degrades leupeptin into three components, acetyl-leucine, leucine and argininal [H]

COG id: COG2234

COG function: function code R; Predicted aminopeptidases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M28 family. M28A subfamily [H]

Homologues:

Organism=Homo sapiens, GI7706387, Length=509, Percent_Identity=26.5225933202358, Blast_Score=120, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008979
- InterPro:   IPR007484
- InterPro:   IPR002884 [H]

Pfam domain/function: PF01483 P_proprotein; PF04389 Peptidase_M28 [H]

EC number: NA

Molecular weight: Translated: 58222; Mature: 58091

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSCLRGAASAAAIALCTFAAPAIAGPAEDKLIAQLLDEGLNRSDAMEIASELMDRIGPR
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
LTNSENHRKAEDWAAAKFASFGLKNIHREPFEFGLGWNLKSYSATMTSPRSLPLTVLPVA
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCEECCCCCCCCEEEEEEE
WSPPTGGTITAPVIVAPMTKVENFDAWKGKLAGRIVLVSLPGETSASADPVFERLSGEEI
ECCCCCCEEECCEEEECHHHCCCCCHHHHCCCCEEEEEECCCCCCCCCCHHHHHHCCHHH
GKLDKYTLPRHDPEGLAMQVARRGFARKLSEFLKAEGALAMVRMTYRDGKLVHGEGYDFV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEECCCCCCC
PGQTLAVPAMDMAQEDYRRLVRLEKTGAAPQLSLSIDASFDDKDLMADNVIAEIPGSDPK
CCCEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHCCHHEECCCCCCC
AGYVMAGAHFDSWIAGDGASDNGAGSVAVIEAARLLSKMGVKPKRTIRFALWSGEEQGLL
CCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCC
GSKAYIEQHLATRPVDPALKGIDSYSAWRNAYPITPKPGYSQLKAYFNMDNGSGKFRGIY
CHHHHHHHHHHCCCCCHHHHCCHHHHHHHCCCCCCCCCCHHHHHEEEECCCCCCCEEEEE
AEGNVAAAPILREWLAPFSSLGADKVVMSKTGGTDHVYLQAIGLPGYQFIQDPLDYESRV
ECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCCHHHHHCCCCHHHHH
HHSSLDTLDHMRADDMRQASVILAGMLLQAATSEKELPRSPLPTKPDATDPFKVQDPNQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TSCLRGAASAAAIALCTFAAPAIAGPAEDKLIAQLLDEGLNRSDAMEIASELMDRIGPR
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
LTNSENHRKAEDWAAAKFASFGLKNIHREPFEFGLGWNLKSYSATMTSPRSLPLTVLPVA
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCEECCCCCCCCEEEEEEE
WSPPTGGTITAPVIVAPMTKVENFDAWKGKLAGRIVLVSLPGETSASADPVFERLSGEEI
ECCCCCCEEECCEEEECHHHCCCCCHHHHCCCCEEEEEECCCCCCCCCCHHHHHHCCHHH
GKLDKYTLPRHDPEGLAMQVARRGFARKLSEFLKAEGALAMVRMTYRDGKLVHGEGYDFV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEECCCCCCC
PGQTLAVPAMDMAQEDYRRLVRLEKTGAAPQLSLSIDASFDDKDLMADNVIAEIPGSDPK
CCCEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHCCHHEECCCCCCC
AGYVMAGAHFDSWIAGDGASDNGAGSVAVIEAARLLSKMGVKPKRTIRFALWSGEEQGLL
CCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCC
GSKAYIEQHLATRPVDPALKGIDSYSAWRNAYPITPKPGYSQLKAYFNMDNGSGKFRGIY
CHHHHHHHHHHCCCCCHHHHCCHHHHHHHCCCCCCCCCCHHHHHEEEECCCCCCCEEEEE
AEGNVAAAPILREWLAPFSSLGADKVVMSKTGGTDHVYLQAIGLPGYQFIQDPLDYESRV
ECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCCHHHHHCCCCHHHHH
HHSSLDTLDHMRADDMRQASVILAGMLLQAATSEKELPRSPLPTKPDATDPFKVQDPNQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA