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The map label for this gene is pit [H]

Identifier: 87198865

GI number: 87198865

Start: 892853

End: 893851

Strand: Direct

Name: pit [H]

Synonym: Saro_0843

Alternate gene names: 87198865

Gene position: 892853-893851 (Clockwise)

Preceding gene: 87198864

Following gene: 87198866

Centisome position: 25.07

GC content: 64.46

Gene sequence:

>999_bases
ATGCACGAGCTCGCCTTTCCGCTGCTGGTCGGCCTGATCGCGCTGGCGCTGGCCTTCGACTTCCTGAACGGCCTCCACGA
CGCCGCCAATTCCATCGCGACCGTCGTGTCGACGCGGCTGCTCTCGCCGGTCGGCGCGGTCATCTTTGCCGCCGGCGGCA
ACTTCGCCGCCTATTGGCTGGTCGGTCTCCACGTTGCCGACACCATCGGCAAAGGCATAATCGACAAGGACATCGTCACG
CCGGCGGTGGTCTTTGGCGCGCTCATCGGAGCGATGTTCTGGAACGTCGTGACCTGGATCAAGGGCATACCCTCTTCGTC
CAGCCACGCGCTTATCGGCGGCCTGCTGGGCGCGGGCGTGATGCACGGCGGCTTCGGCGCGATCGAATCCAAGAAGACGA
TCGAGACTTTCGTGGCGATCTTCATGTCGCCGATGATCGGCTTCGCGCTCGCGATGTTCTTCGTGCTGGTGACGAGCTGG
TTGTTCAGGGGCTTCCAGCCCCGCCGCGCCGAAGGCGTGTTCAAGGGCCTGCACCTCCTCTCGTCGGCGGCATATTCGAT
CAGCCACGGCGGCAACGATGCGCAGAAGACGATGGGCATCATCACCGTCCTGCTCTATTCGACCGGCTACCTGAAGGGTG
AGTTCCATATTCCCGAATGGGTCGTGCTTTCCTGCTATGCCGCCATCTCGCTCGGCACGATGTCCGGTGGGTGGAAGATC
ATCAAGACGATGGGCACCAAGATCACCCGCCTCAATCACCACACTGGTTTCTGCGCCTCGTCGGCGGGTTCGATCGTGGT
GTTCGGGGCAAGCGCGCTCGGCATTCCGGTCTCGACCACCCATGCCATCACCGGCAGCGTGATCGGCACGGGCGCAGCCC
GCCGCGCGAGCGCCGTCCGCTGGGGCGTGGCGCAGCGCGTGGTCATGGCCTGGTTCATCACCATCCCCGCGAGCGCGACG
GTGGGTGCGGGCTTCTATCTCCTGACGCGCCTGTTCTGA

Upstream 100 bases:

>100_bases
GCACCTCGAGAAGATCGCCGACGCGTTCGAGGACGTGGCGAACGAGATCGACGCGCTGGTCATCGACCACGCCTGAAGGC
GCGGCACAGGAGCTGATCCG

Downstream 100 bases:

>100_bases
TCGCGGGCGGTGTTCGCCCTCAGGGGCGATGAATTTTCGATCAAAGGGCTTGGCTTTCCCCGCGGCGGCGGCAAGATGGT
TTCCAGAGAAACCGACCCGG

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MHELAFPLLVGLIALALAFDFLNGLHDAANSIATVVSTRLLSPVGAVIFAAGGNFAAYWLVGLHVADTIGKGIIDKDIVT
PAVVFGALIGAMFWNVVTWIKGIPSSSSHALIGGLLGAGVMHGGFGAIESKKTIETFVAIFMSPMIGFALAMFFVLVTSW
LFRGFQPRRAEGVFKGLHLLSSAAYSISHGGNDAQKTMGIITVLLYSTGYLKGEFHIPEWVVLSCYAAISLGTMSGGWKI
IKTMGTKITRLNHHTGFCASSAGSIVVFGASALGIPVSTTHAITGSVIGTGAARRASAVRWGVAQRVVMAWFITIPASAT
VGAGFYLLTRLF

Sequences:

>Translated_332_residues
MHELAFPLLVGLIALALAFDFLNGLHDAANSIATVVSTRLLSPVGAVIFAAGGNFAAYWLVGLHVADTIGKGIIDKDIVT
PAVVFGALIGAMFWNVVTWIKGIPSSSSHALIGGLLGAGVMHGGFGAIESKKTIETFVAIFMSPMIGFALAMFFVLVTSW
LFRGFQPRRAEGVFKGLHLLSSAAYSISHGGNDAQKTMGIITVLLYSTGYLKGEFHIPEWVVLSCYAAISLGTMSGGWKI
IKTMGTKITRLNHHTGFCASSAGSIVVFGASALGIPVSTTHAITGSVIGTGAARRASAVRWGVAQRVVMAWFITIPASAT
VGAGFYLLTRLF
>Mature_332_residues
MHELAFPLLVGLIALALAFDFLNGLHDAANSIATVVSTRLLSPVGAVIFAAGGNFAAYWLVGLHVADTIGKGIIDKDIVT
PAVVFGALIGAMFWNVVTWIKGIPSSSSHALIGGLLGAGVMHGGFGAIESKKTIETFVAIFMSPMIGFALAMFFVLVTSW
LFRGFQPRRAEGVFKGLHLLSSAAYSISHGGNDAQKTMGIITVLLYSTGYLKGEFHIPEWVVLSCYAAISLGTMSGGWKI
IKTMGTKITRLNHHTGFCASSAGSIVVFGASALGIPVSTTHAITGSVIGTGAARRASAVRWGVAQRVVMAWFITIPASAT
VGAGFYLLTRLF

Specific function: Low-affinity inorganic phosphate transport (Probable) [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=160, Percent_Identity=33.75, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI5803173, Length=146, Percent_Identity=34.2465753424658, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1789360, Length=219, Percent_Identity=30.5936073059361, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789907, Length=217, Percent_Identity=31.7972350230415, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI25146401, Length=165, Percent_Identity=30.3030303030303, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71981576, Length=165, Percent_Identity=26.6666666666667, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17536725, Length=228, Percent_Identity=27.6315789473684, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17539280, Length=175, Percent_Identity=26.2857142857143, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI32566716, Length=165, Percent_Identity=26.6666666666667, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17557328, Length=160, Percent_Identity=27.5, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6319773, Length=155, Percent_Identity=27.741935483871, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21356511, Length=184, Percent_Identity=27.1739130434783, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 34823; Mature: 34823

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHELAFPLLVGLIALALAFDFLNGLHDAANSIATVVSTRLLSPVGAVIFAAGGNFAAYWL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHH
VGLHVADTIGKGIIDKDIVTPAVVFGALIGAMFWNVVTWIKGIPSSSSHALIGGLLGAGV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
MHGGFGAIESKKTIETFVAIFMSPMIGFALAMFFVLVTSWLFRGFQPRRAEGVFKGLHLL
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
SSAAYSISHGGNDAQKTMGIITVLLYSTGYLKGEFHIPEWVVLSCYAAISLGTMSGGWKI
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCCCHHH
IKTMGTKITRLNHHTGFCASSAGSIVVFGASALGIPVSTTHAITGSVIGTGAARRASAVR
HHHHCCHHEEECCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHCCHHCCCHHHHHHHHH
WGVAQRVVMAWFITIPASATVGAGFYLLTRLF
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MHELAFPLLVGLIALALAFDFLNGLHDAANSIATVVSTRLLSPVGAVIFAAGGNFAAYWL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHH
VGLHVADTIGKGIIDKDIVTPAVVFGALIGAMFWNVVTWIKGIPSSSSHALIGGLLGAGV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
MHGGFGAIESKKTIETFVAIFMSPMIGFALAMFFVLVTSWLFRGFQPRRAEGVFKGLHLL
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
SSAAYSISHGGNDAQKTMGIITVLLYSTGYLKGEFHIPEWVVLSCYAAISLGTMSGGWKI
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCCCHHH
IKTMGTKITRLNHHTGFCASSAGSIVVFGASALGIPVSTTHAITGSVIGTGAARRASAVR
HHHHCCHHEEECCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHCCHHCCCHHHHHHHHH
WGVAQRVVMAWFITIPASATVGAGFYLLTRLF
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9696772; 11481430 [H]