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The map label for this gene is thlA [H]

Identifier: 87198831

GI number: 87198831

Start: 861245

End: 862423

Strand: Direct

Name: thlA [H]

Synonym: Saro_0809

Alternate gene names: 87198831

Gene position: 861245-862423 (Clockwise)

Preceding gene: 87198830

Following gene: 87198832

Centisome position: 24.18

GC content: 68.19

Gene sequence:

>1179_bases
ATGACCGACATCTACATCGTCTCGGGCGCGCGTACCGCCATCGGCTCGTTCGGCGGCGGGCTCGCTTCGCTGCGTCCGGC
AGAGAGCGGCGCCATCGTGATCAAGGAAGCCATCGCCCGCGCCGGGATCGCGCCGGACAAGGTGCAGAACGTGGTGATCG
GCACGGTGGTGCCGACCCAGCCCAAGGACGCCTACGTCAGCCGCGTGGCCGCGGTGAATGCCGGCATTCCGATCGAAGCC
CCCGCGATGAACGTGAACCGCCTGTGCGGTTCGGGCCTGCAGGCGATCGTCTCGGCCGCACAGGGCATTGCCCTTGGCGA
GCAGGACATCGCCATCGGCGGCGGCGCGGAAAGCATGTCGAACGCGCCGCACATGGTGCTGACCGCGCGCAACGGCCAGA
AGATGGGCGACCAGGTGCTGATGGACGCCATGCTCGGCGCGCTGCACGATCCGTTCGAAGGTATCCACATGGGCGTGACC
GCGGAGAACGTGGCCGAGCGCTGCCAGATCACGCGCGAGGAGCAGGACTCGCTGGCGGTTGAGAGCCACAAGCGCGCGGC
AGCGGCCATCGCGGCTGGCTACTTCAAGGAGCAGATCGTCCCGGTCGAGATCAAGACGCGCAAGGGCGTTGTCGTGTTCG
ACACCGACGAGCACGTCCGCGCCGATGCCTCGGTCGAGGCGATGGCGGGGCTGAAGCCGGTGTTCAAGAAGGACGGCACG
GTCACGGCCGGCAACGCCAGCGGCATCAACGACGGCGCGGCCGCCGTGGTGCTGGCGAGCGGCAAGGCGGTTGAAGAGCA
CGGCCTGAAGCCGATGGCCAGGATCCTCGGCTGGGGTCACGCGGGCGTCGAGCCGAACGTGATGGGTCTCGGCCCGGTCA
AGGCCGTGCCGGTGGCGCTGCAGCGCGCAGGGCTTGACCTCGACCAGATCGACGTGATCGAGGCCAACGAGGCCTTCGCC
GCGCAGGCTTGCGGCGTCGCCAAGGAACTGGGCTTCGACCCCGCCAAGACCAACCCCAACGGCTCGGGCATTTCGCTGGG
TCATCCCATCGGTGCGACCGGCGCGATCCTGACGATCAAGACGATGTACGAGCTGCACCGCACCGGCGGGCGCTATGGCC
TGATCACCATGTGCATCGGCGGTGGCCAGGGCATCGCCATGGTCATCGAGAGGGTCTGA

Upstream 100 bases:

>100_bases
GCTCGGCCTGCGCAAGCTGCGCAACAAGAGCCTCGAGAATCCGTGGAAGAAGCACGACAACATTCCGCTCTGACGAGCGC
TTCAGTCTGGAAGAACTGAC

Downstream 100 bases:

>100_bases
ACATGAAACTGGGCCGCCTCAACCACGTTGGCGTCGCGACGCCGGACATCGACGCTTCCATCGCGTTCTATCGCGACGTG
ATGGGGGCGACCGACATCAC

Product: beta-ketothiolase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MTDIYIVSGARTAIGSFGGGLASLRPAESGAIVIKEAIARAGIAPDKVQNVVIGTVVPTQPKDAYVSRVAAVNAGIPIEA
PAMNVNRLCGSGLQAIVSAAQGIALGEQDIAIGGGAESMSNAPHMVLTARNGQKMGDQVLMDAMLGALHDPFEGIHMGVT
AENVAERCQITREEQDSLAVESHKRAAAAIAAGYFKEQIVPVEIKTRKGVVVFDTDEHVRADASVEAMAGLKPVFKKDGT
VTAGNASGINDGAAAVVLASGKAVEEHGLKPMARILGWGHAGVEPNVMGLGPVKAVPVALQRAGLDLDQIDVIEANEAFA
AQACGVAKELGFDPAKTNPNGSGISLGHPIGATGAILTIKTMYELHRTGGRYGLITMCIGGGQGIAMVIERV

Sequences:

>Translated_392_residues
MTDIYIVSGARTAIGSFGGGLASLRPAESGAIVIKEAIARAGIAPDKVQNVVIGTVVPTQPKDAYVSRVAAVNAGIPIEA
PAMNVNRLCGSGLQAIVSAAQGIALGEQDIAIGGGAESMSNAPHMVLTARNGQKMGDQVLMDAMLGALHDPFEGIHMGVT
AENVAERCQITREEQDSLAVESHKRAAAAIAAGYFKEQIVPVEIKTRKGVVVFDTDEHVRADASVEAMAGLKPVFKKDGT
VTAGNASGINDGAAAVVLASGKAVEEHGLKPMARILGWGHAGVEPNVMGLGPVKAVPVALQRAGLDLDQIDVIEANEAFA
AQACGVAKELGFDPAKTNPNGSGISLGHPIGATGAILTIKTMYELHRTGGRYGLITMCIGGGQGIAMVIERV
>Mature_391_residues
TDIYIVSGARTAIGSFGGGLASLRPAESGAIVIKEAIARAGIAPDKVQNVVIGTVVPTQPKDAYVSRVAAVNAGIPIEAP
AMNVNRLCGSGLQAIVSAAQGIALGEQDIAIGGGAESMSNAPHMVLTARNGQKMGDQVLMDAMLGALHDPFEGIHMGVTA
ENVAERCQITREEQDSLAVESHKRAAAAIAAGYFKEQIVPVEIKTRKGVVVFDTDEHVRADASVEAMAGLKPVFKKDGTV
TAGNASGINDGAAAVVLASGKAVEEHGLKPMARILGWGHAGVEPNVMGLGPVKAVPVALQRAGLDLDQIDVIEANEAFAA
QACGVAKELGFDPAKTNPNGSGISLGHPIGATGAILTIKTMYELHRTGGRYGLITMCIGGGQGIAMVIERV

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=391, Percent_Identity=46.8030690537084, Blast_Score=350, Evalue=1e-96,
Organism=Homo sapiens, GI167614485, Length=388, Percent_Identity=43.5567010309278, Blast_Score=348, Evalue=6e-96,
Organism=Homo sapiens, GI4501853, Length=396, Percent_Identity=40.6565656565657, Blast_Score=268, Evalue=8e-72,
Organism=Homo sapiens, GI4557237, Length=398, Percent_Identity=39.9497487437186, Blast_Score=264, Evalue=1e-70,
Organism=Homo sapiens, GI4504327, Length=424, Percent_Identity=30.8962264150943, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI194353979, Length=391, Percent_Identity=30.4347826086957, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI1788554, Length=402, Percent_Identity=48.5074626865672, Blast_Score=328, Evalue=3e-91,
Organism=Escherichia coli, GI87082165, Length=392, Percent_Identity=45.1530612244898, Blast_Score=325, Evalue=3e-90,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=44.8877805486284, Blast_Score=324, Evalue=5e-90,
Organism=Escherichia coli, GI48994986, Length=399, Percent_Identity=38.5964912280702, Blast_Score=231, Evalue=8e-62,
Organism=Escherichia coli, GI1788683, Length=423, Percent_Identity=32.8605200945626, Blast_Score=194, Evalue=9e-51,
Organism=Caenorhabditis elegans, GI133906874, Length=390, Percent_Identity=44.1025641025641, Blast_Score=330, Evalue=8e-91,
Organism=Caenorhabditis elegans, GI25147385, Length=390, Percent_Identity=40, Blast_Score=275, Evalue=4e-74,
Organism=Caenorhabditis elegans, GI17535921, Length=399, Percent_Identity=36.0902255639098, Blast_Score=232, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17535917, Length=393, Percent_Identity=32.824427480916, Blast_Score=186, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17551802, Length=428, Percent_Identity=29.9065420560748, Blast_Score=162, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6325229, Length=400, Percent_Identity=40.25, Blast_Score=268, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6322031, Length=396, Percent_Identity=34.0909090909091, Blast_Score=204, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24655093, Length=393, Percent_Identity=48.3460559796438, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI17648125, Length=391, Percent_Identity=42.7109974424552, Blast_Score=315, Evalue=3e-86,
Organism=Drosophila melanogaster, GI24640423, Length=398, Percent_Identity=39.1959798994975, Blast_Score=267, Evalue=8e-72,
Organism=Drosophila melanogaster, GI17137578, Length=428, Percent_Identity=31.5420560747664, Blast_Score=184, Evalue=9e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 40174; Mature: 40042

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00098 THIOLASE_1 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIYIVSGARTAIGSFGGGLASLRPAESGAIVIKEAIARAGIAPDKVQNVVIGTVVPTQ
CCEEEEECCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHCEEEEEEECCCC
PKDAYVSRVAAVNAGIPIEAPAMNVNRLCGSGLQAIVSAAQGIALGEQDIAIGGGAESMS
CHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCCEEECCCHHHHC
NAPHMVLTARNGQKMGDQVLMDAMLGALHDPFEGIHMGVTAENVAERCQITREEQDSLAV
CCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHCCCCHHHHHHH
ESHKRAAAAIAAGYFKEQIVPVEIKTRKGVVVFDTDEHVRADASVEAMAGLKPVFKKDGT
HHHHHHHHHHHHHHHHHCEEEEEEECCCCEEEEECCCCCCCCCCHHHHHCCCHHHHCCCC
VTAGNASGINDGAAAVVLASGKAVEEHGLKPMARILGWGHAGVEPNVMGLGPVKAVPVAL
EEECCCCCCCCCCEEEEEECCCCHHHHCCCHHHHHHCCCCCCCCCCEECCCCHHHHHHHH
QRAGLDLDQIDVIEANEAFAAQACGVAKELGFDPAKTNPNGSGISLGHPIGATGAILTIK
HHCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCEEEHH
TMYELHRTGGRYGLITMCIGGGQGIAMVIERV
HHHHHHHCCCCEEEEEEEEECCCCCEEEEECC
>Mature Secondary Structure 
TDIYIVSGARTAIGSFGGGLASLRPAESGAIVIKEAIARAGIAPDKVQNVVIGTVVPTQ
CEEEEECCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHCEEEEEEECCCC
PKDAYVSRVAAVNAGIPIEAPAMNVNRLCGSGLQAIVSAAQGIALGEQDIAIGGGAESMS
CHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCCEEECCCHHHHC
NAPHMVLTARNGQKMGDQVLMDAMLGALHDPFEGIHMGVTAENVAERCQITREEQDSLAV
CCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHCCCCHHHHHHH
ESHKRAAAAIAAGYFKEQIVPVEIKTRKGVVVFDTDEHVRADASVEAMAGLKPVFKKDGT
HHHHHHHHHHHHHHHHHCEEEEEEECCCCEEEEECCCCCCCCCCHHHHHCCCHHHHCCCC
VTAGNASGINDGAAAVVLASGKAVEEHGLKPMARILGWGHAGVEPNVMGLGPVKAVPVAL
EEECCCCCCCCCCEEEEEECCCCHHHHCCCHHHHHHCCCCCCCCCCEECCCCHHHHHHHH
QRAGLDLDQIDVIEANEAFAAQACGVAKELGFDPAKTNPNGSGISLGHPIGATGAILTIK
HHCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCEEEHH
TMYELHRTGGRYGLITMCIGGGQGIAMVIERV
HHHHHHHCCCCEEEEEEEEECCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]