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The map label for this gene is prfC [H]

Identifier: 87198714

GI number: 87198714

Start: 729333

End: 730856

Strand: Direct

Name: prfC [H]

Synonym: Saro_0690

Alternate gene names: 87198714

Gene position: 729333-730856 (Clockwise)

Preceding gene: 87198713

Following gene: 87198715

Centisome position: 20.48

GC content: 63.85

Gene sequence:

>1524_bases
ATGACTTCCAACCGCCGCACCTTCGCCATCATCTCGCACCCTGACGCCGGTAAGACCACGCTTACCGAAAAGCTGCTGCT
TCAGGGCGGCGCGATCCATCTGGCCGGCGAGGTCAAGGCGCGCGGTGCGGCCCGTCGCGCCCGTTCCGACTGGATGAAGA
TCGAGCAGCAGCGCGGCATCTCCGTCACTTCCTCGGTGATGACCTTCCAGAAGGACGGCATCGTCTTCAACCTGCTCGAT
ACCCCGGGCCACGAAGACTTTTCGGAAGACACCTACCGCACGCTGACCGCCGTCGACTCCGCCATCATGGTGATCGACGC
GGCCAAGGGTATCGAGCCGCAGACGCGCAAGCTGTTCGAGGTCTGCCGCATGCGGTCGGTTCCGATCATCACTTTCGTCA
ACAAGGTCGACCGCGAGGGGCGTTCGGTGTTCGAAACGCTGGACGAGGTGGCCGACGCGCTCGCGCTCGATGTCGTTCCG
ATGTCCTGGCCCATCGGCATGGGCGGCGTCTTCCAGGGCGTGATGAACTTCGCGGACGAGACCATCGCCCGTCCCGAAGG
CGACAGCCGCGAGTTTCTCGGCAAGCGCGAACCGGCAATCGACATCCCTGATGACGTCGCCGAGGAAATCGAGCTTGCCC
GCGCCGGCTATCCCGAATTCGATCTCGAAGCCTATCGCCACGGCGACCTGACGCCGGTCTACTTCGGCTCCGCGCTCAAG
AATTTCGGCGTGGCCGAGCTGATCGATGCCATTGCCAAGTACGCACCGCCGCCGCGCCCGCAATCGTCCGACATTGGCAC
GGTCGACCCGGAACGGCCCGACGTCACGGGGTTCATCTTCAAGGTCCAGGCCAACATGGACCCCCAGCACCGCGACCGCA
TTGCCTTCATGCGCATGGTCTCGGGCACGTTCCGGCGCGGCATGAAGCTTACGCCTTCGGGGCTGGGCAAGCCGATCGCG
ATCCATTCGCCGATCCTGTTCTTCGCGCAGGACCGCGAGATCGCCGATACTGCCGAGGCCGGCGACATCATCGGCATTCC
GAACCACGGCACCCTGCGCGTGGGCGATACCCTGTCGGAAAAGAACGACCTGCGCTTCACGGGCCTGCCCAACTTTGCGC
CGGAAATCCTGCGCCGCGTGGTGCTCAAGGATCCGACCAAGACCAAGCAGCTAAGGAAGGCGCTCGACGACCTGTCCGAG
GAAGGGGTCATCCAGGTGTTCTATCCGGAGATCGGCAGCCAGTGGATCGTGGGCGTGGTCGGCCAGCTCCAGCTCGACGT
GCTCGTCTCGCGTCTTGAGGCGGAATACAAGGTCGAGGCCGTGCTGGAACCCTCGCCATTCGACACTGCGCGCTGGCTCA
AGGGGAGCGAAGCTGCGCTCAAGTCCTTTGCCGATTTCAACGTGTCGAACCTTGCCAAGGATCGCGACGGCGACCCGGTG
TTCATGGCTCGCTCGGCCTGGGACGTGGGCTACCAGCAGGAACGCAACCCCGAACTCACCTTCTCTGCGACCAAGGAAAG
GTAG

Upstream 100 bases:

>100_bases
CTGGGAGCGGAACTGCGCCGATAGCTCGGCGCAGAGCCGCCTTCCCGGCGACGGCCACCGTGGCCGCCAGCCTGCGCTCC
TTGTCTGAACCGAGTACCCG

Downstream 100 bases:

>100_bases
TTCCGCCTTAGTCGGCCCGGGGGGTTGAAACCGCAGGGCGAAGGGCGCAGTTAGCCTCCCATGCAGTTACTCGGTCCGAC
CTCGAACACCTTCATCTCCC

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MTSNRRTFAIISHPDAGKTTLTEKLLLQGGAIHLAGEVKARGAARRARSDWMKIEQQRGISVTSSVMTFQKDGIVFNLLD
TPGHEDFSEDTYRTLTAVDSAIMVIDAAKGIEPQTRKLFEVCRMRSVPIITFVNKVDREGRSVFETLDEVADALALDVVP
MSWPIGMGGVFQGVMNFADETIARPEGDSREFLGKREPAIDIPDDVAEEIELARAGYPEFDLEAYRHGDLTPVYFGSALK
NFGVAELIDAIAKYAPPPRPQSSDIGTVDPERPDVTGFIFKVQANMDPQHRDRIAFMRMVSGTFRRGMKLTPSGLGKPIA
IHSPILFFAQDREIADTAEAGDIIGIPNHGTLRVGDTLSEKNDLRFTGLPNFAPEILRRVVLKDPTKTKQLRKALDDLSE
EGVIQVFYPEIGSQWIVGVVGQLQLDVLVSRLEAEYKVEAVLEPSPFDTARWLKGSEAALKSFADFNVSNLAKDRDGDPV
FMARSAWDVGYQQERNPELTFSATKER

Sequences:

>Translated_507_residues
MTSNRRTFAIISHPDAGKTTLTEKLLLQGGAIHLAGEVKARGAARRARSDWMKIEQQRGISVTSSVMTFQKDGIVFNLLD
TPGHEDFSEDTYRTLTAVDSAIMVIDAAKGIEPQTRKLFEVCRMRSVPIITFVNKVDREGRSVFETLDEVADALALDVVP
MSWPIGMGGVFQGVMNFADETIARPEGDSREFLGKREPAIDIPDDVAEEIELARAGYPEFDLEAYRHGDLTPVYFGSALK
NFGVAELIDAIAKYAPPPRPQSSDIGTVDPERPDVTGFIFKVQANMDPQHRDRIAFMRMVSGTFRRGMKLTPSGLGKPIA
IHSPILFFAQDREIADTAEAGDIIGIPNHGTLRVGDTLSEKNDLRFTGLPNFAPEILRRVVLKDPTKTKQLRKALDDLSE
EGVIQVFYPEIGSQWIVGVVGQLQLDVLVSRLEAEYKVEAVLEPSPFDTARWLKGSEAALKSFADFNVSNLAKDRDGDPV
FMARSAWDVGYQQERNPELTFSATKER
>Mature_506_residues
TSNRRTFAIISHPDAGKTTLTEKLLLQGGAIHLAGEVKARGAARRARSDWMKIEQQRGISVTSSVMTFQKDGIVFNLLDT
PGHEDFSEDTYRTLTAVDSAIMVIDAAKGIEPQTRKLFEVCRMRSVPIITFVNKVDREGRSVFETLDEVADALALDVVPM
SWPIGMGGVFQGVMNFADETIARPEGDSREFLGKREPAIDIPDDVAEEIELARAGYPEFDLEAYRHGDLTPVYFGSALKN
FGVAELIDAIAKYAPPPRPQSSDIGTVDPERPDVTGFIFKVQANMDPQHRDRIAFMRMVSGTFRRGMKLTPSGLGKPIAI
HSPILFFAQDREIADTAEAGDIIGIPNHGTLRVGDTLSEKNDLRFTGLPNFAPEILRRVVLKDPTKTKQLRKALDDLSEE
GVIQVFYPEIGSQWIVGVVGQLQLDVLVSRLEAEYKVEAVLEPSPFDTARWLKGSEAALKSFADFNVSNLAKDRDGDPVF
MARSAWDVGYQQERNPELTFSATKER

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides but is not codon-specific. It may interact directly with the ribosome. The stimulation of RF-1 and RF-2 is significantly re

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=497, Percent_Identity=26.5593561368209, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI19923640, Length=506, Percent_Identity=26.4822134387352, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI25306283, Length=391, Percent_Identity=27.8772378516624, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI25306287, Length=288, Percent_Identity=29.8611111111111, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI157426893, Length=181, Percent_Identity=29.2817679558011, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI94966754, Length=141, Percent_Identity=31.9148936170213, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI310132016, Length=122, Percent_Identity=36.0655737704918, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI310110807, Length=122, Percent_Identity=36.0655737704918, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI310123363, Length=122, Percent_Identity=36.0655737704918, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI4503483, Length=152, Percent_Identity=31.5789473684211, Blast_Score=76, Evalue=1e-13,
Organism=Escherichia coli, GI1790835, Length=521, Percent_Identity=50.2879078694818, Blast_Score=513, Evalue=1e-146,
Organism=Escherichia coli, GI1789738, Length=499, Percent_Identity=29.8597194388778, Blast_Score=155, Evalue=8e-39,
Organism=Escherichia coli, GI48994988, Length=223, Percent_Identity=29.5964125560538, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788922, Length=173, Percent_Identity=31.2138728323699, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17533571, Length=481, Percent_Identity=27.8586278586279, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17556745, Length=477, Percent_Identity=25.5765199161426, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17557151, Length=135, Percent_Identity=32.5925925925926, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=31.5789473684211, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=31.5789473684211, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17506493, Length=165, Percent_Identity=27.8787878787879, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=477, Percent_Identity=25.9958071278826, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6322359, Length=514, Percent_Identity=26.8482490272374, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6323320, Length=174, Percent_Identity=28.735632183908, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324707, Length=152, Percent_Identity=32.2368421052632, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320593, Length=152, Percent_Identity=32.2368421052632, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24582462, Length=481, Percent_Identity=27.8586278586279, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI221458488, Length=505, Percent_Identity=26.9306930693069, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI78706572, Length=136, Percent_Identity=36.0294117647059, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24585711, Length=155, Percent_Identity=32.258064516129, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24585713, Length=155, Percent_Identity=32.258064516129, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24585709, Length=155, Percent_Identity=32.258064516129, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 56174; Mature: 56043

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSNRRTFAIISHPDAGKTTLTEKLLLQGGAIHLAGEVKARGAARRARSDWMKIEQQRGI
CCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCC
SVTSSVMTFQKDGIVFNLLDTPGHEDFSEDTYRTLTAVDSAIMVIDAAKGIEPQTRKLFE
CHHHHHHHEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHH
VCRMRSVPIITFVNKVDREGRSVFETLDEVADALALDVVPMSWPIGMGGVFQGVMNFADE
HHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHH
TIARPEGDSREFLGKREPAIDIPDDVAEEIELARAGYPEFDLEAYRHGDLTPVYFGSALK
HHCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCHHHHHHHHH
NFGVAELIDAIAKYAPPPRPQSSDIGTVDPERPDVTGFIFKVQANMDPQHRDRIAFMRMV
HCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHH
SGTFRRGMKLTPSGLGKPIAIHSPILFFAQDREIADTAEAGDIIGIPNHGTLRVGDTLSE
HHHHHCCCCCCCCCCCCCEEECCCEEEEECCCCHHHHCCCCCEEECCCCCCEEECCCCCC
KNDLRFTGLPNFAPEILRRVVLKDPTKTKQLRKALDDLSEEGVIQVFYPEIGSQWIVGVV
CCCEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHH
GQLQLDVLVSRLEAEYKVEAVLEPSPFDTARWLKGSEAALKSFADFNVSNLAKDRDGDPV
HHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHCCCCHHHHHHHHCCCHHHHHCCCCCCEE
FMARSAWDVGYQQERNPELTFSATKER
EEEECHHCCCCCCCCCCCEEEEECCCC
>Mature Secondary Structure 
TSNRRTFAIISHPDAGKTTLTEKLLLQGGAIHLAGEVKARGAARRARSDWMKIEQQRGI
CCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCC
SVTSSVMTFQKDGIVFNLLDTPGHEDFSEDTYRTLTAVDSAIMVIDAAKGIEPQTRKLFE
CHHHHHHHEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHH
VCRMRSVPIITFVNKVDREGRSVFETLDEVADALALDVVPMSWPIGMGGVFQGVMNFADE
HHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHH
TIARPEGDSREFLGKREPAIDIPDDVAEEIELARAGYPEFDLEAYRHGDLTPVYFGSALK
HHCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCHHHHHHHHH
NFGVAELIDAIAKYAPPPRPQSSDIGTVDPERPDVTGFIFKVQANMDPQHRDRIAFMRMV
HCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHH
SGTFRRGMKLTPSGLGKPIAIHSPILFFAQDREIADTAEAGDIIGIPNHGTLRVGDTLSE
HHHHHCCCCCCCCCCCCCEEECCCEEEEECCCCHHHHCCCCCEEECCCCCCEEECCCCCC
KNDLRFTGLPNFAPEILRRVVLKDPTKTKQLRKALDDLSEEGVIQVFYPEIGSQWIVGVV
CCCEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHH
GQLQLDVLVSRLEAEYKVEAVLEPSPFDTARWLKGSEAALKSFADFNVSNLAKDRDGDPV
HHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHCCCCHHHHHHHHCCCHHHHHCCCCCCEE
FMARSAWDVGYQQERNPELTFSATKER
EEEECHHCCCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7539688 [H]