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The map label for this gene is dapE

Identifier: 87198605

GI number: 87198605

Start: 627580

End: 628707

Strand: Direct

Name: dapE

Synonym: Saro_0581

Alternate gene names: 87198605

Gene position: 627580-628707 (Clockwise)

Preceding gene: 87198604

Following gene: 87198609

Centisome position: 17.62

GC content: 68.44

Gene sequence:

>1128_bases
ATGACCGACCTTGATCCCGTCGTCCTGGCCGAACGGCTGATCGACTGCCCCAGCATCACGCCGGCCACCGGCGCGGTTTT
CGATTGCCTCCAGGCCATGCTCGAACCGCTCGGTTTCGCGATCCACCGCTTCGTCGCGGGCGAAGCGCCCGACGGTCCGG
TCGAGAATCTCTTCGCCATCCGCAAGGGCCCCGAAGGCGCGCGCCACTTCGCCTTTGCCGGCCACCTTGACGTCGTCCCG
CCAGGCGAGGGCTGGACGTCCGGCCCGTTCAAGGCAGAGCGCCGGGGCGAACTGCTCTACGGGCGCGGCGCGGTCGACAT
GAAGGGTTCGATCGCCGCGATGGTCGCAGCCGTGGCCGAAATTCCGGCGGACGCGGGCACGCTCAGCTTCATCATCACCG
GCGACGAGGAAGGCCCCGCCCGCTACGGCACCGTCCCCCTCATCGACCTCATCCGCCAGCTCGGGGCCGAGCCGGACCTT
TGCCTGGTGGGCGAGCCCACGTCGGTCCACCGCCTGGGAGACATGGTGAAGATCGGGCGGCGCGGTTCGGTCAACATGTG
GATCGCCTGCAAGGGCGCGCAGGGCCATGTCGCCTATCCCCACCTGGCCGACAACCCGATCCCCCGCCTCGTCGCGCTGC
TGGCAGATCTCGATGCGCTCGTGCTCGACGGCGGCACCGAGTGGTTCCAGCCTTCCAACCTCGAGATCACCGACCTCGAG
GTCGGCAATCCCGCCACCAACGTCATCCCCGCCGAAGCCCGCGCGCGCATCTCGATCCGCTTCAATGACCGCCATACCGG
CGCCGAGCTGGTCGCCAGGGTCGAGGAGATCGCCCATCGCCACAAGGGCGAGGTCCGCGCGGTCATCTCGGGCGAGAGTT
TCATCACCCTGCCCGGCGCCTTTTCCGCGATGATCGCCGATGCGGTGAAGGCCGAAACCGGGCTCGATCCCGAACTGTCC
ACCAGCGGCGGCACGTCCGACGCTCGCTTCCTGCGCGCGGTCTGCCCGGTCGTCGAGTTCGGCCTGTGCAACGCCACGAT
GCACAAGAAGGACGAGGCCGTCGCTATGGAAGACCTCCGCGTCCTCCAGCGCATCTACCGGCGGATTGCCCTCAGCGCCC
TTTCCTGA

Upstream 100 bases:

>100_bases
ATATGGAAGCGGTCTGGACCCACGACTGGATGCAGGCCTGGATCGAGGCGGCCGAGCACGAGGACTGGACGATCGAACAG
TTCGAGGAGCCCGCGCGCTG

Downstream 100 bases:

>100_bases
GCACGATGTAGACCGCGCCCGCCCCGCCGTGCCGAGGCTGGGCAGGGCGTACGGCGGCGATGTTCGATGCGTGAGCGCTG
TGCGCCAGCCAGTCGAGCAG

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MTDLDPVVLAERLIDCPSITPATGAVFDCLQAMLEPLGFAIHRFVAGEAPDGPVENLFAIRKGPEGARHFAFAGHLDVVP
PGEGWTSGPFKAERRGELLYGRGAVDMKGSIAAMVAAVAEIPADAGTLSFIITGDEEGPARYGTVPLIDLIRQLGAEPDL
CLVGEPTSVHRLGDMVKIGRRGSVNMWIACKGAQGHVAYPHLADNPIPRLVALLADLDALVLDGGTEWFQPSNLEITDLE
VGNPATNVIPAEARARISIRFNDRHTGAELVARVEEIAHRHKGEVRAVISGESFITLPGAFSAMIADAVKAETGLDPELS
TSGGTSDARFLRAVCPVVEFGLCNATMHKKDEAVAMEDLRVLQRIYRRIALSALS

Sequences:

>Translated_375_residues
MTDLDPVVLAERLIDCPSITPATGAVFDCLQAMLEPLGFAIHRFVAGEAPDGPVENLFAIRKGPEGARHFAFAGHLDVVP
PGEGWTSGPFKAERRGELLYGRGAVDMKGSIAAMVAAVAEIPADAGTLSFIITGDEEGPARYGTVPLIDLIRQLGAEPDL
CLVGEPTSVHRLGDMVKIGRRGSVNMWIACKGAQGHVAYPHLADNPIPRLVALLADLDALVLDGGTEWFQPSNLEITDLE
VGNPATNVIPAEARARISIRFNDRHTGAELVARVEEIAHRHKGEVRAVISGESFITLPGAFSAMIADAVKAETGLDPELS
TSGGTSDARFLRAVCPVVEFGLCNATMHKKDEAVAMEDLRVLQRIYRRIALSALS
>Mature_374_residues
TDLDPVVLAERLIDCPSITPATGAVFDCLQAMLEPLGFAIHRFVAGEAPDGPVENLFAIRKGPEGARHFAFAGHLDVVPP
GEGWTSGPFKAERRGELLYGRGAVDMKGSIAAMVAAVAEIPADAGTLSFIITGDEEGPARYGTVPLIDLIRQLGAEPDLC
LVGEPTSVHRLGDMVKIGRRGSVNMWIACKGAQGHVAYPHLADNPIPRLVALLADLDALVLDGGTEWFQPSNLEITDLEV
GNPATNVIPAEARARISIRFNDRHTGAELVARVEEIAHRHKGEVRAVISGESFITLPGAFSAMIADAVKAETGLDPELST
SGGTSDARFLRAVCPVVEFGLCNATMHKKDEAVAMEDLRVLQRIYRRIALSALS

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=374, Percent_Identity=40.3743315508021, Blast_Score=258, Evalue=5e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_NOVAD (Q2GAU5)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_495862.1
- ProteinModelPortal:   Q2GAU5
- SMR:   Q2GAU5
- STRING:   Q2GAU5
- GeneID:   3915593
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_0581
- eggNOG:   COG0624
- HOGENOM:   HBG728841
- OMA:   PMVFEDT
- PhylomeDB:   Q2GAU5
- ProtClustDB:   PRK13009
- BioCyc:   NARO279238:SARO_0581-MONOMER
- GO:   GO:0006508
- HAMAP:   MF_01690
- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 39992; Mature: 39861

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 77-77 ACT_SITE 136-136

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLDPVVLAERLIDCPSITPATGAVFDCLQAMLEPLGFAIHRFVAGEAPDGPVENLFAI
CCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
RKGPEGARHFAFAGHLDVVPPGEGWTSGPFKAERRGELLYGRGAVDMKGSIAAMVAAVAE
CCCCCCCCEEEEECCEEECCCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHH
IPADAGTLSFIITGDEEGPARYGTVPLIDLIRQLGAEPDLCLVGEPTSVHRLGDMVKIGR
CCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCC
RGSVNMWIACKGAQGHVAYPHLADNPIPRLVALLADLDALVLDGGTEWFQPSNLEITDLE
CCCCEEEEEEECCCCCEECCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEEEE
VGNPATNVIPAEARARISIRFNDRHTGAELVARVEEIAHRHKGEVRAVISGESFITLPGA
CCCCCCCEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCH
FSAMIADAVKAETGLDPELSTSGGTSDARFLRAVCPVVEFGLCNATMHKKDEAVAMEDLR
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHH
VLQRIYRRIALSALS
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDLDPVVLAERLIDCPSITPATGAVFDCLQAMLEPLGFAIHRFVAGEAPDGPVENLFAI
CCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
RKGPEGARHFAFAGHLDVVPPGEGWTSGPFKAERRGELLYGRGAVDMKGSIAAMVAAVAE
CCCCCCCCEEEEECCEEECCCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHH
IPADAGTLSFIITGDEEGPARYGTVPLIDLIRQLGAEPDLCLVGEPTSVHRLGDMVKIGR
CCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCC
RGSVNMWIACKGAQGHVAYPHLADNPIPRLVALLADLDALVLDGGTEWFQPSNLEITDLE
CCCCEEEEEEECCCCCEECCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEEEE
VGNPATNVIPAEARARISIRFNDRHTGAELVARVEEIAHRHKGEVRAVISGESFITLPGA
CCCCCCCEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCH
FSAMIADAVKAETGLDPELSTSGGTSDARFLRAVCPVVEFGLCNATMHKKDEAVAMEDLR
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHH
VLQRIYRRIALSALS
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA