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The map label for this gene is yhcR [H]

Identifier: 87198583

GI number: 87198583

Start: 607830

End: 609581

Strand: Direct

Name: yhcR [H]

Synonym: Saro_0559

Alternate gene names: 87198583

Gene position: 607830-609581 (Clockwise)

Preceding gene: 87198581

Following gene: 87198587

Centisome position: 17.07

GC content: 66.95

Gene sequence:

>1752_bases
ATGATCTCGCGCTTTCGTCCCGCCCTTGCATTGCTCCTCTCCGGCTGCCTCGGGGCTTGCGCCGCGCCGATGGCGGAAGG
CGGGCATGGAGAGCCGGTCGAAGTCGGCATCGTTGCGATAAACGACTTCCATGGCGCGCTCGAGCCGCCCCGCCAATCGG
TCAACGTGACCGATGCCGCAGGCAACATCCTGCCCGTGCCGGCGGGCGGCGCGGCATGGCTTGCCTCGGCGGTGGATTCA
GTTCGGGCGGCGCATCCTCATTCGCTGACGGTATCGGCCGGCGACATGATCTCCGCCTCGCAACTTGCCTCCTCGCTCTA
TCTGGACGAGCCGGCGATCGGCGTGATGAACCGCATCGGCGTGGATTTCAACGCGGTCGGCAATCACGAGTTCGACCGGG
GGCAGGATGAACTCCTGCGCATGCAGAACGGCGGATGCGAGAAGTTCACCGCCAGGCAACCCTGCGCGCTCGAACCCTTT
GCCGGCGCGACGTTCAGGTATCTGGCCGCCAGCACCCGCCGCGCCGATGGATCGACGCTGTTTCCGGCCACGGGCCTGCG
CACATTCGGCAGCGGCCGTCGCGCCGTCACTGTCGGCGTGATCGGGCTCACGCTCAAGGGTACGCCGAATCTGGTCAATC
CGACCGGTATCAAGGGGCTGACGTTCGAGGACGAGGCCGACACGATCAACGCCGCCGTTCCCGTGCTCAAGGCAAAGGGC
GCCGACGCCATCGTTGTCCTGATCCACGAGGGTGGCCGCACAAAGGGCAATCCGGATCCGCAGGGGTGCAACGACTTTGC
CGGAGACATTCGCCCGATCCTCGACCGGCTGGATACGCGCGTCGATGTGGTCGTCTCGGGCCACACGCACTGGGCCTACG
TCTGCGACTACGCGGCGCTAAACCCCGCCAAGCCGATCCTCCTGACCAGCGCGGGGGTATTTGGCGAGCTGGTGACCGAC
ATAAGCCTGAAGATCGACCCGGTGGCGGGGCGCGTGGTGTCGAAGCAGGCGCGCAATGTCATCGTTCAATCGGTGCCCTA
CACCGCGACCCGCGGCCCCATTTCGAACACGCCGCTTTTCCCGCAGTTCCAGCCGCGCGCCGACGTGGCCGCGTATGTAG
CGAAGTATGTCGAGGCGTCGAAGGAATACACCCAGCGCCCGGTCGGCCGGATCGACCGCGCCGCGCCCAAGAACGAGGGC
GCGGAAGGCGGCGCGGGCGGCCCCTTGGGCAACCTGATCGCCGACGCGCAACTGGGCGCGACGCGCGATGCCGGGGCTCA
GATCGCCTTCATGAACCCCTTCGGCATTCGCGCTCCGTTGGTCCCGGCCGCCGATGGCACGCTCACATTCGGCGACATCT
ACCTGTGCCAGCCCTTCGGCAACAACGTGGTGACCGAGACCATGACCGGAGCCGAGATCAAGGCGGTGCTGGAAGAAGGC
CTCGACGACAACGGGCCCAGGCAGGCGCTCGCCCCGTCAACGGGCTTCGAGTTCCGCTACGACCCGGCGCGCACCTCGGG
CGACCGCATCGTTTCGATCACGCTGGATGGCCAGCCGCTCGACATGACGAGGACCTACCGCGTGACCGTCAACGGCTTCC
TCGGCCTCGGCGGCGACGGGTTCTCGGGCTTCGTCGGAAAGCCCGACACGGTAACCGGCCCGACCGACATCGATGCGCTG
GAAAGCTGGATCAAGGCCGTGCCGGTGCGCAACGTGCCGCAGGAGGTGCGGGCGCTGGTGGTAAAGGGGTAA

Upstream 100 bases:

>100_bases
CGCTGAAGGAAAAGATGCGATCCCTGCCTTCTGTCTGCAACCGTGTTCGGGTAGGGGCCACTCAACCGGTTACGCTCGTT
ACGAAAAGGTCGCCCGCCTG

Downstream 100 bases:

>100_bases
GATGGTCCTTGCGCCGGCCTAACCGCTTTTCGGCCACCAGGGCACGAACCCCGACGTCCGGCGGACATACTCGGCATAGC
CTGGCCGCCGTTCCGCCATG

Product: 5'-nucleotidase

Products: ribonucleoside; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 583; Mature: 583

Protein sequence:

>583_residues
MISRFRPALALLLSGCLGACAAPMAEGGHGEPVEVGIVAINDFHGALEPPRQSVNVTDAAGNILPVPAGGAAWLASAVDS
VRAAHPHSLTVSAGDMISASQLASSLYLDEPAIGVMNRIGVDFNAVGNHEFDRGQDELLRMQNGGCEKFTARQPCALEPF
AGATFRYLAASTRRADGSTLFPATGLRTFGSGRRAVTVGVIGLTLKGTPNLVNPTGIKGLTFEDEADTINAAVPVLKAKG
ADAIVVLIHEGGRTKGNPDPQGCNDFAGDIRPILDRLDTRVDVVVSGHTHWAYVCDYAALNPAKPILLTSAGVFGELVTD
ISLKIDPVAGRVVSKQARNVIVQSVPYTATRGPISNTPLFPQFQPRADVAAYVAKYVEASKEYTQRPVGRIDRAAPKNEG
AEGGAGGPLGNLIADAQLGATRDAGAQIAFMNPFGIRAPLVPAADGTLTFGDIYLCQPFGNNVVTETMTGAEIKAVLEEG
LDDNGPRQALAPSTGFEFRYDPARTSGDRIVSITLDGQPLDMTRTYRVTVNGFLGLGGDGFSGFVGKPDTVTGPTDIDAL
ESWIKAVPVRNVPQEVRALVVKG

Sequences:

>Translated_583_residues
MISRFRPALALLLSGCLGACAAPMAEGGHGEPVEVGIVAINDFHGALEPPRQSVNVTDAAGNILPVPAGGAAWLASAVDS
VRAAHPHSLTVSAGDMISASQLASSLYLDEPAIGVMNRIGVDFNAVGNHEFDRGQDELLRMQNGGCEKFTARQPCALEPF
AGATFRYLAASTRRADGSTLFPATGLRTFGSGRRAVTVGVIGLTLKGTPNLVNPTGIKGLTFEDEADTINAAVPVLKAKG
ADAIVVLIHEGGRTKGNPDPQGCNDFAGDIRPILDRLDTRVDVVVSGHTHWAYVCDYAALNPAKPILLTSAGVFGELVTD
ISLKIDPVAGRVVSKQARNVIVQSVPYTATRGPISNTPLFPQFQPRADVAAYVAKYVEASKEYTQRPVGRIDRAAPKNEG
AEGGAGGPLGNLIADAQLGATRDAGAQIAFMNPFGIRAPLVPAADGTLTFGDIYLCQPFGNNVVTETMTGAEIKAVLEEG
LDDNGPRQALAPSTGFEFRYDPARTSGDRIVSITLDGQPLDMTRTYRVTVNGFLGLGGDGFSGFVGKPDTVTGPTDIDAL
ESWIKAVPVRNVPQEVRALVVKG
>Mature_583_residues
MISRFRPALALLLSGCLGACAAPMAEGGHGEPVEVGIVAINDFHGALEPPRQSVNVTDAAGNILPVPAGGAAWLASAVDS
VRAAHPHSLTVSAGDMISASQLASSLYLDEPAIGVMNRIGVDFNAVGNHEFDRGQDELLRMQNGGCEKFTARQPCALEPF
AGATFRYLAASTRRADGSTLFPATGLRTFGSGRRAVTVGVIGLTLKGTPNLVNPTGIKGLTFEDEADTINAAVPVLKAKG
ADAIVVLIHEGGRTKGNPDPQGCNDFAGDIRPILDRLDTRVDVVVSGHTHWAYVCDYAALNPAKPILLTSAGVFGELVTD
ISLKIDPVAGRVVSKQARNVIVQSVPYTATRGPISNTPLFPQFQPRADVAAYVAKYVEASKEYTQRPVGRIDRAAPKNEG
AEGGAGGPLGNLIADAQLGATRDAGAQIAFMNPFGIRAPLVPAADGTLTFGDIYLCQPFGNNVVTETMTGAEIKAVLEEG
LDDNGPRQALAPSTGFEFRYDPARTSGDRIVSITLDGQPLDMTRTYRVTVNGFLGLGGDGFSGFVGKPDTVTGPTDIDAL
ESWIKAVPVRNVPQEVRALVVKG

Specific function: Sugar-nonspecific endonuclease that yields nucleotide 3'-monophosphate products. No 5'-nucleotidase activity was detected, using 5'-AMP as the substrate, in the presence of diverse divalent metals and with various pH values [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TNase-like domain [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=603, Percent_Identity=25.0414593698176, Blast_Score=104, Evalue=3e-22,
Organism=Escherichia coli, GI1786687, Length=580, Percent_Identity=24.6551724137931, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24641187, Length=577, Percent_Identity=25.6499133448873, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI28573524, Length=561, Percent_Identity=22.8163992869875, Blast_Score=86, Evalue=8e-17,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019948
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR016027
- InterPro:   IPR004365
- InterPro:   IPR016071
- InterPro:   IPR006021 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00746 Gram_pos_anchor; PF00149 Metallophos; PF00565 SNase; PF01336 tRNA_anti [H]

EC number: 3.1.3.5

Molecular weight: Translated: 61029; Mature: 61029

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00785 5_NUCLEOTIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISRFRPALALLLSGCLGACAAPMAEGGHGEPVEVGIVAINDFHGALEPPRQSVNVTDAA
CCCCHHHHHHHHHHHHHHHHHCHHCCCCCCCCEEEEEEEEECCCCCCCCCCCCCEEEECC
GNILPVPAGGAAWLASAVDSVRAAHPHSLTVSAGDMISASQLASSLYLDEPAIGVMNRIG
CCEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHCC
VDFNAVGNHEFDRGQDELLRMQNGGCEKFTARQPCALEPFAGATFRYLAASTRRADGSTL
CCEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEE
FPATGLRTFGSGRRAVTVGVIGLTLKGTPNLVNPTGIKGLTFEDEADTINAAVPVLKAKG
CCCCCCHHCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCEEECCCCCCHHHHHCEEECCC
ADAIVVLIHEGGRTKGNPDPQGCNDFAGDIRPILDRLDTRVDVVVSGHTHWAYVCDYAAL
CCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCEEEEEEHHHC
NPAKPILLTSAGVFGELVTDISLKIDPVAGRVVSKQARNVIVQSVPYTATRGPISNTPLF
CCCCCEEEECCCHHHHHHHCCEEEECCCHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCC
PQFQPRADVAAYVAKYVEASKEYTQRPVGRIDRAAPKNEGAEGGAGGPLGNLIADAQLGA
CCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCC
TRDAGAQIAFMNPFGIRAPLVPAADGTLTFGDIYLCQPFGNNVVTETMTGAEIKAVLEEG
CCCCCCEEEEECCCCCCCCCCCCCCCEEEECEEEEECCCCCCEEEECCCCHHHHHHHHHC
LDDNGPRQALAPSTGFEFRYDPARTSGDRIVSITLDGQPLDMTRTYRVTVNGFLGLGGDG
CCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEEECCCCCCCEEEEEEEEEEEEECCCCC
FSGFVGKPDTVTGPTDIDALESWIKAVPVRNVPQEVRALVVKG
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHEEEECC
>Mature Secondary Structure
MISRFRPALALLLSGCLGACAAPMAEGGHGEPVEVGIVAINDFHGALEPPRQSVNVTDAA
CCCCHHHHHHHHHHHHHHHHHCHHCCCCCCCCEEEEEEEEECCCCCCCCCCCCCEEEECC
GNILPVPAGGAAWLASAVDSVRAAHPHSLTVSAGDMISASQLASSLYLDEPAIGVMNRIG
CCEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHCC
VDFNAVGNHEFDRGQDELLRMQNGGCEKFTARQPCALEPFAGATFRYLAASTRRADGSTL
CCEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEE
FPATGLRTFGSGRRAVTVGVIGLTLKGTPNLVNPTGIKGLTFEDEADTINAAVPVLKAKG
CCCCCCHHCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCEEECCCCCCHHHHHCEEECCC
ADAIVVLIHEGGRTKGNPDPQGCNDFAGDIRPILDRLDTRVDVVVSGHTHWAYVCDYAAL
CCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCEEEEEEHHHC
NPAKPILLTSAGVFGELVTDISLKIDPVAGRVVSKQARNVIVQSVPYTATRGPISNTPLF
CCCCCEEEECCCHHHHHHHCCEEEECCCHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCC
PQFQPRADVAAYVAKYVEASKEYTQRPVGRIDRAAPKNEGAEGGAGGPLGNLIADAQLGA
CCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCC
TRDAGAQIAFMNPFGIRAPLVPAADGTLTFGDIYLCQPFGNNVVTETMTGAEIKAVLEEG
CCCCCCEEEEECCCCCCCCCCCCCCCEEEECEEEEECCCCCCEEEECCCCHHHHHHHHHC
LDDNGPRQALAPSTGFEFRYDPARTSGDRIVSITLDGQPLDMTRTYRVTVNGFLGLGGDG
CCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEEECCCCCCCEEEEEEEEEEEEECCCCC
FSGFVGKPDTVTGPTDIDALESWIKAVPVRNVPQEVRALVVKG
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5'-ribonucleotide; H2O

Specific reaction: A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]