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The map label for this gene is yngF [H]

Identifier: 87198527

GI number: 87198527

Start: 544498

End: 545304

Strand: Direct

Name: yngF [H]

Synonym: Saro_0502

Alternate gene names: 87198527

Gene position: 544498-545304 (Clockwise)

Preceding gene: 87198526

Following gene: 87198528

Centisome position: 15.29

GC content: 65.68

Gene sequence:

>807_bases
ATGCCGAACATGAACTTCCACGACCGCGTCAGCGTTACCATCGAAGACCACGTCGCCCACGTCCTGCTAGACCGGGCGGA
CAAGATGAACGCGCTCGACGATGCCATGTTCGAAGGCATCGTTGCGGCGGGCCACCACCTCCATTCCGTGAAGGGCGTCC
GTTGCGTGGTGCTTTCCGGTGCCGGGCGGTCGTTCTGTGCCGGCCTCGACCTTTCCAGCATGGGCCGCACCGACCGCTGG
AGCGGCAATTCGCTGACCGAGAGGACGCATGGCAATGCCAACCGCGCGCAGCAGGCGGCGATGGTCTGGCGCAAGCTTCC
CATGCCGGTGATCGCCGCAGTCCACGGCGTATGCTTCGGCGGCGGTCTGCAGGTTGCCAGCGGCGCCGATATCCGCTTCA
TCACGCCGGACGCGCGGCTGGCGGTGATGGAAGTGAAGTGGGGCCTCGTGCCTGACATGGCGGGCTATGCGCTCTGGCGC
GGCAACGTGCGCGACGACGTCCTGCGCGAACTGACCTACACCCACCGCGAATTCAGCGGCGAGGAAGCGGTGCGCTTCGG
CTTTGCCACCCACGTCGCGGACGATCCCCTTGCCGGCGCCATGGAGCTTGCCAGGGTCGTCGCGGAAAAGAGCCCCAATG
CCGTGCGCGGGGCCAAGACGCTCTCCAACCGGGCGCCGGACCTCACCGTCGACGATGTGCTCATGGCCGAATCGATCGCC
CAGCACGAGCTGATGTATAGCCGCAACCAGATGGAAGCGGTGAAGGCAGGCATGGAAAAGCGAGCCGGGGATTTCGTCGA
TCCATGA

Upstream 100 bases:

>100_bases
CTGCGGGGAGGGCCTTTCGCTTTACGGCGATCGGTCAATAGAGGAAGCTGGACCCCGCCGCCGGGCTTGGGCAACATCGG
CGCAATCAGCAAGAGAGCGG

Downstream 100 bases:

>100_bases
CCCTGACGGCAATGACCCAGGTTAAAGGTCCGGCATGAGAGCCATCCACGATCCCCACGGCGCGCTGGCCGGCGGGCTTG
CCGCCATCCGCGCGCAGTAC

Product: enoyl-CoA hydratase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MPNMNFHDRVSVTIEDHVAHVLLDRADKMNALDDAMFEGIVAAGHHLHSVKGVRCVVLSGAGRSFCAGLDLSSMGRTDRW
SGNSLTERTHGNANRAQQAAMVWRKLPMPVIAAVHGVCFGGGLQVASGADIRFITPDARLAVMEVKWGLVPDMAGYALWR
GNVRDDVLRELTYTHREFSGEEAVRFGFATHVADDPLAGAMELARVVAEKSPNAVRGAKTLSNRAPDLTVDDVLMAESIA
QHELMYSRNQMEAVKAGMEKRAGDFVDP

Sequences:

>Translated_268_residues
MPNMNFHDRVSVTIEDHVAHVLLDRADKMNALDDAMFEGIVAAGHHLHSVKGVRCVVLSGAGRSFCAGLDLSSMGRTDRW
SGNSLTERTHGNANRAQQAAMVWRKLPMPVIAAVHGVCFGGGLQVASGADIRFITPDARLAVMEVKWGLVPDMAGYALWR
GNVRDDVLRELTYTHREFSGEEAVRFGFATHVADDPLAGAMELARVVAEKSPNAVRGAKTLSNRAPDLTVDDVLMAESIA
QHELMYSRNQMEAVKAGMEKRAGDFVDP
>Mature_267_residues
PNMNFHDRVSVTIEDHVAHVLLDRADKMNALDDAMFEGIVAAGHHLHSVKGVRCVVLSGAGRSFCAGLDLSSMGRTDRWS
GNSLTERTHGNANRAQQAAMVWRKLPMPVIAAVHGVCFGGGLQVASGADIRFITPDARLAVMEVKWGLVPDMAGYALWRG
NVRDDVLRELTYTHREFSGEEAVRFGFATHVADDPLAGAMELARVVAEKSPNAVRGAKTLSNRAPDLTVDDVLMAESIAQ
HELMYSRNQMEAVKAGMEKRAGDFVDP

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI70995211, Length=272, Percent_Identity=29.7794117647059, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI194097323, Length=257, Percent_Identity=25.2918287937743, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI4502327, Length=246, Percent_Identity=26.4227642276423, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=259, Percent_Identity=24.7104247104247, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI221142681, Length=261, Percent_Identity=25.6704980842912, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788682, Length=196, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787660, Length=258, Percent_Identity=30.6201550387597, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1790281, Length=182, Percent_Identity=25.2747252747253, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17536985, Length=270, Percent_Identity=27.7777777777778, Blast_Score=110, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17534483, Length=261, Percent_Identity=28.3524904214559, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17540714, Length=226, Percent_Identity=26.9911504424779, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17554946, Length=252, Percent_Identity=27.7777777777778, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25145438, Length=255, Percent_Identity=24.7058823529412, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17560910, Length=194, Percent_Identity=26.2886597938144, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19920382, Length=230, Percent_Identity=31.7391304347826, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24653139, Length=251, Percent_Identity=26.6932270916335, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI20129971, Length=255, Percent_Identity=26.2745098039216, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24653477, Length=255, Percent_Identity=26.2745098039216, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 29164; Mature: 29032

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNMNFHDRVSVTIEDHVAHVLLDRADKMNALDDAMFEGIVAAGHHLHSVKGVRCVVLSG
CCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
AGRSFCAGLDLSSMGRTDRWSGNSLTERTHGNANRAQQAAMVWRKLPMPVIAAVHGVCFG
CCCHHEECCCHHHCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCC
GGLQVASGADIRFITPDARLAVMEVKWGLVPDMAGYALWRGNVRDDVLRELTYTHREFSG
CCEEEECCCCEEEECCCCEEEEEEECCCCCCCHHHHHEECCCCHHHHHHHHHHHHHHCCC
EEAVRFGFATHVADDPLAGAMELARVVAEKSPNAVRGAKTLSNRAPDLTVDDVLMAESIA
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
QHELMYSRNQMEAVKAGMEKRAGDFVDP
HHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PNMNFHDRVSVTIEDHVAHVLLDRADKMNALDDAMFEGIVAAGHHLHSVKGVRCVVLSG
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
AGRSFCAGLDLSSMGRTDRWSGNSLTERTHGNANRAQQAAMVWRKLPMPVIAAVHGVCFG
CCCHHEECCCHHHCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCC
GGLQVASGADIRFITPDARLAVMEVKWGLVPDMAGYALWRGNVRDDVLRELTYTHREFSG
CCEEEECCCCEEEECCCCEEEEEEECCCCCCCHHHHHEECCCCHHHHHHHHHHHHHHCCC
EEAVRFGFATHVADDPLAGAMELARVVAEKSPNAVRGAKTLSNRAPDLTVDDVLMAESIA
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
QHELMYSRNQMEAVKAGMEKRAGDFVDP
HHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]