Click here to switch to the map view.

The map label for this gene is rstB [H]

Identifier: 87198467

GI number: 87198467

Start: 481428

End: 482825

Strand: Direct

Name: rstB [H]

Synonym: Saro_0442

Alternate gene names: 87198467

Gene position: 481428-482825 (Clockwise)

Preceding gene: 87198466

Following gene: 87198468

Centisome position: 13.52

GC content: 68.96

Gene sequence:

>1398_bases
GTGCGAGCCTTGCGCCTCTGGCCCCGTAGCCTGCAGGGACAGGTCCTGCTGGCGATTGCCTTCGCCCTGCTGATCGCGCA
GACGATCTCGGCGGTGCTGATCTGGCGCGAACAGAGCCACCGCCGCGAATCCGGGATGGTCAGCTCCGCCGCGTTCCGGT
TGCTCGGCCCGCCGCCCGAAATGCGGCGCAAGCATCACGGCGATGACGACGAGCGTCCGCCGCCGCCACCCTTCCGCGTC
GACGTGACCGACCGTTCCCCCCAGCGCCCCGGCGAGCGCCGCCTCCCGCGCGTCGAGCACGAGCTTGCAGAGGTCCTCAC
CGCGCAGGGATATGACGCGGCCCAGGTTGTCGTGTTCCAGCGCGATCCGCTGCAAGACGAGCCGAGCCTTGAATTCATGC
GCCGCCACCGGCGCGAACTCGCGGCGGTCTCGAAGGAACCGATGCCTCGCCTGGTCATCGCCGCTGTTCGCCAGTCGAGC
GGGCAATGGGTTTCGACCCGCGTGTTCAGCGAAGGCGGCGAGATGCGCGTGCTTGCCCCACTCGTCGGGCAGACGATCGT
GCTCTACTTCGTGCTGGTCGGCGTCATGGCGCTTATCCTGCGCCGCATCACCCGCCCGCTTAAGGCGCTGACCCGCCGCG
TCGGCGATTTCGCCAGCAATCGTACGCTCGATGGACAGCTGGAACCCGAAGGGCCGGAGGATATCCAGGGCCTGATCGAC
GCGCACAACGCGATGGAAGCCCGGATCGCGGCCATGCTCGACGAGAAGGACGTCATGCTCGGCGCCATCGGGCACGACCT
CAAGACCCCTCTCGCCGCCCTGCGCGTGCGCATAGAGGCAGTCGACGACGATACCGAGCGCGCGCGCATGGCAGCCACCA
TCGAGGACATCAACCGCTCGCTCGACGACATTCTCTCGCTCGCCCGCGTCGGTCGCCCTTCCGACCCGGTGGAGCCGACG
GAACTTTCCGCTCTCGTCGCCGACGTGATCGGCGAATTCGAGGACATGGGGGAACCGGTCGAACTGGGCGACACCGAACG
CGTCGTCGTTCCCCTGCGCGCCACCTGGCTGCGCCGCGCGCTGCGCAACCTCGTTTCCAACGCCATGCGCTATGGCGAAA
GGGCGCGCGTATCGCTGGCCCGTGACGGGCGCACCGCCGTTCTCCGGATCGAGGACGATGGCCCCGGCATCCCCGAAGGC
GAGATCGCGCGCATGATGGAGCCGTTCACGCGGCTGGAGGCATCGCGCAACAGCGGTACCGGCGGCACGGGCCTCGGCCT
CACGCTTGCCCGCGCCATTGCGGACCAGCACGGTGGGACCCTGATCCTGACGAACCGCCACGGCCCCGGCGGCGAAATCG
TCGGCCTGACCGCCACGGTGCGCCTGCCGACGGGTTAA

Upstream 100 bases:

>100_bases
CAGCCGCAATCCGGAACTGATCCAGACCGTCTGGGGTGGCGGCTACATGCTCGCCGCCGAAGTCCGCAAGCGCGCCGCCG
TCGAAGGCGAAGCCTGAACC

Downstream 100 bases:

>100_bases
CTCCCCGCTCATCGGCGCGGGGCATAGTCAACCGACATCGGTCGGCGCATGATTGTCGTTCGTCGAAAGGACTCGCGGCC
AGCCCGACCGGTTGCTAGTG

Product: periplasmic sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MRALRLWPRSLQGQVLLAIAFALLIAQTISAVLIWREQSHRRESGMVSSAAFRLLGPPPEMRRKHHGDDDERPPPPPFRV
DVTDRSPQRPGERRLPRVEHELAEVLTAQGYDAAQVVVFQRDPLQDEPSLEFMRRHRRELAAVSKEPMPRLVIAAVRQSS
GQWVSTRVFSEGGEMRVLAPLVGQTIVLYFVLVGVMALILRRITRPLKALTRRVGDFASNRTLDGQLEPEGPEDIQGLID
AHNAMEARIAAMLDEKDVMLGAIGHDLKTPLAALRVRIEAVDDDTERARMAATIEDINRSLDDILSLARVGRPSDPVEPT
ELSALVADVIGEFEDMGEPVELGDTERVVVPLRATWLRRALRNLVSNAMRYGERARVSLARDGRTAVLRIEDDGPGIPEG
EIARMMEPFTRLEASRNSGTGGTGLGLTLARAIADQHGGTLILTNRHGPGGEIVGLTATVRLPTG

Sequences:

>Translated_465_residues
MRALRLWPRSLQGQVLLAIAFALLIAQTISAVLIWREQSHRRESGMVSSAAFRLLGPPPEMRRKHHGDDDERPPPPPFRV
DVTDRSPQRPGERRLPRVEHELAEVLTAQGYDAAQVVVFQRDPLQDEPSLEFMRRHRRELAAVSKEPMPRLVIAAVRQSS
GQWVSTRVFSEGGEMRVLAPLVGQTIVLYFVLVGVMALILRRITRPLKALTRRVGDFASNRTLDGQLEPEGPEDIQGLID
AHNAMEARIAAMLDEKDVMLGAIGHDLKTPLAALRVRIEAVDDDTERARMAATIEDINRSLDDILSLARVGRPSDPVEPT
ELSALVADVIGEFEDMGEPVELGDTERVVVPLRATWLRRALRNLVSNAMRYGERARVSLARDGRTAVLRIEDDGPGIPEG
EIARMMEPFTRLEASRNSGTGGTGLGLTLARAIADQHGGTLILTNRHGPGGEIVGLTATVRLPTG
>Mature_465_residues
MRALRLWPRSLQGQVLLAIAFALLIAQTISAVLIWREQSHRRESGMVSSAAFRLLGPPPEMRRKHHGDDDERPPPPPFRV
DVTDRSPQRPGERRLPRVEHELAEVLTAQGYDAAQVVVFQRDPLQDEPSLEFMRRHRRELAAVSKEPMPRLVIAAVRQSS
GQWVSTRVFSEGGEMRVLAPLVGQTIVLYFVLVGVMALILRRITRPLKALTRRVGDFASNRTLDGQLEPEGPEDIQGLID
AHNAMEARIAAMLDEKDVMLGAIGHDLKTPLAALRVRIEAVDDDTERARMAATIEDINRSLDDILSLARVGRPSDPVEPT
ELSALVADVIGEFEDMGEPVELGDTERVVVPLRATWLRRALRNLVSNAMRYGERARVSLARDGRTAVLRIEDDGPGIPEG
EIARMMEPFTRLEASRNSGTGGTGLGLTLARAIADQHGGTLILTNRHGPGGEIVGLTATVRLPTG

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=251, Percent_Identity=31.8725099601594, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1789808, Length=277, Percent_Identity=28.5198555956679, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1790551, Length=269, Percent_Identity=29.368029739777, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788393, Length=264, Percent_Identity=26.8939393939394, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87082128, Length=308, Percent_Identity=26.2987012987013, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1786783, Length=291, Percent_Identity=25.7731958762887, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786912, Length=218, Percent_Identity=29.8165137614679, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790346, Length=221, Percent_Identity=26.6968325791855, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1788279, Length=288, Percent_Identity=25.6944444444444, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI87081816, Length=358, Percent_Identity=25.4189944134078, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI48994928, Length=230, Percent_Identity=24.7826086956522, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI145693157, Length=234, Percent_Identity=24.3589743589744, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51250; Mature: 51250

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRALRLWPRSLQGQVLLAIAFALLIAQTISAVLIWREQSHRRESGMVSSAAFRLLGPPPE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHH
MRRKHHGDDDERPPPPPFRVDVTDRSPQRPGERRLPRVEHELAEVLTAQGYDAAQVVVFQ
HHHHCCCCCCCCCCCCCEEEEECCCCCCCCCHHCCCHHHHHHHHHHHHCCCCCEEEEEEE
RDPLQDEPSLEFMRRHRRELAAVSKEPMPRLVIAAVRQSSGQWVSTRVFSEGGEMRVLAP
CCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEHHHCCCCCEEEEHH
LVGQTIVLYFVLVGVMALILRRITRPLKALTRRVGDFASNRTLDGQLEPEGPEDIQGLID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
AHNAMEARIAAMLDEKDVMLGAIGHDLKTPLAALRVRIEAVDDDTERARMAATIEDINRS
HHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHH
LDDILSLARVGRPSDPVEPTELSALVADVIGEFEDMGEPVELGDTERVVVPLRATWLRRA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHH
LRNLVSNAMRYGERARVSLARDGRTAVLRIEDDGPGIPEGEIARMMEPFTRLEASRNSGT
HHHHHHHHHHCCCHHEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCC
GGTGLGLTLARAIADQHGGTLILTNRHGPGGEIVGLTATVRLPTG
CCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEEECCCC
>Mature Secondary Structure
MRALRLWPRSLQGQVLLAIAFALLIAQTISAVLIWREQSHRRESGMVSSAAFRLLGPPPE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHH
MRRKHHGDDDERPPPPPFRVDVTDRSPQRPGERRLPRVEHELAEVLTAQGYDAAQVVVFQ
HHHHCCCCCCCCCCCCCEEEEECCCCCCCCCHHCCCHHHHHHHHHHHHCCCCCEEEEEEE
RDPLQDEPSLEFMRRHRRELAAVSKEPMPRLVIAAVRQSSGQWVSTRVFSEGGEMRVLAP
CCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEHHHCCCCCEEEEHH
LVGQTIVLYFVLVGVMALILRRITRPLKALTRRVGDFASNRTLDGQLEPEGPEDIQGLID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
AHNAMEARIAAMLDEKDVMLGAIGHDLKTPLAALRVRIEAVDDDTERARMAATIEDINRS
HHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHH
LDDILSLARVGRPSDPVEPTELSALVADVIGEFEDMGEPVELGDTERVVVPLRATWLRRA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHH
LRNLVSNAMRYGERARVSLARDGRTAVLRIEDDGPGIPEGEIARMMEPFTRLEASRNSGT
HHHHHHHHHHCCCHHEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCC
GGTGLGLTLARAIADQHGGTLILTNRHGPGGEIVGLTATVRLPTG
CCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]