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The map label for this gene is tolB

Identifier: 87198418

GI number: 87198418

Start: 429429

End: 430784

Strand: Direct

Name: tolB

Synonym: Saro_0393

Alternate gene names: 87198418

Gene position: 429429-430784 (Clockwise)

Preceding gene: 87198417

Following gene: 87198419

Centisome position: 12.06

GC content: 63.94

Gene sequence:

>1356_bases
ATGACGCTGCGCATGCTGTTCGCCTTCGCCTTGCTCGCCGCCGCCCCTGCACAGGCGCAGCAGACCGAGCCGCAACCGGC
CGAAGAAGGCGGGCTTTCCGGTACGGTGTCGGATGACAGCGAGTGGCAGGATCTCGGGATCGCGATCCCGGCCTTTGCGA
CCAACGCCGATGTGCCGACGCCGACCAGCGCAGGGTCGACGTCAGCATTGGGCCAGGCGCTCGCCCAGGTGATCAGCGCC
AACCTCAGGAACAACGGGCTATTCAAGCCGGTCGGGCCTGAAAGCCTGCCAAGGCCCGGTTTCGAACAGGTTCAGGCTCC
CGATTTCGCGGTGTGGTCCGCTCGCAGCGCGGAGATGCTCGTCCAGGGCTATGTGCGAGCCAACGGGGATGGGCAACTGA
CGGTTGGATGCTACCTCTATGACGTTGCGTTAAAGCAGCAACTCGAGAAAGCGGGCTGGACCGTTGCCCCTGCGGACTGG
CGCCGTGCGGCGCACAAGTGCGCGGACATGATCTATGCCCGCCTCTCGGGCGAGAGCCCTTTCTTCGATAGCCGCGTTGC
TTACATCGCTGAGTCCGGTCCCAAGGATCGCCGCATGAAGCGCCTGGCCATCATGGATTCGGACGGGGCCAACCACCGCT
ATCTGACGAGTGGGCAGGCAACTGCACTGACCCCGCGCTTTTCGCCGGATTACAAGTCGATCCTGTATCTGTCGTATCTC
AACGGTCGCCCGCGCATCTATGTCTATGATCTGGCGGCCAATACCCAGCGCCTCGTGACGGAAACGCCAGGTCCCACCTT
CGCCCCGCGCTGGTCGCCCGACGGCAAGTGGATCCTCTATTCGATGGCGACTGCTGGCAATACCGACATCTACAAGGTGC
CGGCGAGCGGCGGGGCCAGTCAGCGCCTTACCGACACCCCGGGAATCGATGTCGGCGGCAGCTTCTCCCCCGACGGGTCG
AGGATCGTCTTCGAAAGCGATCGGTCCGGCAGCCAGCAAATCTACGTGATGAACGCCGATGGTTCCGACCATCGGCGCAT
CAGCTTCTTCGGCGGGCGCGCCGCGACACCGGAATGGAGCCCGCGCGGTGACCAGATCGCATTTACGCACATAGTCGGCA
ATTTGCGCATAGCCGTCGTGAGCCCCGGTGGAGGGGACCTGCGTTATCTGACGGATAGCTGGCAGGATGAGGCGCCAACC
TGGTCACCCAATGGCCGGATCATCCAGTTTTTCCGCACCGAGAAGGGCTCAGGCAAGGCCAGTGTCTGGCAGGTCGATCT
TACCGGTCGCAACGAGCGGCGCTTGTCGACGCCGGGCGACGCCTCCGACCCTGCCTGGGGACCGTTGTTGCCGTGA

Upstream 100 bases:

>100_bases
TCCGTGCGGTGCAGCTGGCGGCACCGTTCAATCTCCCCGCCGAATACCACGATGCGTGGAAGCGCGTCGCCTCCTTCCGA
TTTGACAGGAAATTGTCGCA

Downstream 100 bases:

>100_bases
TAGGCCTTTACCGGCCACCGCAGTGCCATTGCCGGCGCGCGCGAATCAATTTGTCATTTTGCAGGTCGGCCAATGGGAAA
TCGACCGAACGCGAAATTGG

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MTLRMLFAFALLAAAPAQAQQTEPQPAEEGGLSGTVSDDSEWQDLGIAIPAFATNADVPTPTSAGSTSALGQALAQVISA
NLRNNGLFKPVGPESLPRPGFEQVQAPDFAVWSARSAEMLVQGYVRANGDGQLTVGCYLYDVALKQQLEKAGWTVAPADW
RRAAHKCADMIYARLSGESPFFDSRVAYIAESGPKDRRMKRLAIMDSDGANHRYLTSGQATALTPRFSPDYKSILYLSYL
NGRPRIYVYDLAANTQRLVTETPGPTFAPRWSPDGKWILYSMATAGNTDIYKVPASGGASQRLTDTPGIDVGGSFSPDGS
RIVFESDRSGSQQIYVMNADGSDHRRISFFGGRAATPEWSPRGDQIAFTHIVGNLRIAVVSPGGGDLRYLTDSWQDEAPT
WSPNGRIIQFFRTEKGSGKASVWQVDLTGRNERRLSTPGDASDPAWGPLLP

Sequences:

>Translated_451_residues
MTLRMLFAFALLAAAPAQAQQTEPQPAEEGGLSGTVSDDSEWQDLGIAIPAFATNADVPTPTSAGSTSALGQALAQVISA
NLRNNGLFKPVGPESLPRPGFEQVQAPDFAVWSARSAEMLVQGYVRANGDGQLTVGCYLYDVALKQQLEKAGWTVAPADW
RRAAHKCADMIYARLSGESPFFDSRVAYIAESGPKDRRMKRLAIMDSDGANHRYLTSGQATALTPRFSPDYKSILYLSYL
NGRPRIYVYDLAANTQRLVTETPGPTFAPRWSPDGKWILYSMATAGNTDIYKVPASGGASQRLTDTPGIDVGGSFSPDGS
RIVFESDRSGSQQIYVMNADGSDHRRISFFGGRAATPEWSPRGDQIAFTHIVGNLRIAVVSPGGGDLRYLTDSWQDEAPT
WSPNGRIIQFFRTEKGSGKASVWQVDLTGRNERRLSTPGDASDPAWGPLLP
>Mature_450_residues
TLRMLFAFALLAAAPAQAQQTEPQPAEEGGLSGTVSDDSEWQDLGIAIPAFATNADVPTPTSAGSTSALGQALAQVISAN
LRNNGLFKPVGPESLPRPGFEQVQAPDFAVWSARSAEMLVQGYVRANGDGQLTVGCYLYDVALKQQLEKAGWTVAPADWR
RAAHKCADMIYARLSGESPFFDSRVAYIAESGPKDRRMKRLAIMDSDGANHRYLTSGQATALTPRFSPDYKSILYLSYLN
GRPRIYVYDLAANTQRLVTETPGPTFAPRWSPDGKWILYSMATAGNTDIYKVPASGGASQRLTDTPGIDVGGSFSPDGSR
IVFESDRSGSQQIYVMNADGSDHRRISFFGGRAATPEWSPRGDQIAFTHIVGNLRIAVVSPGGGDLRYLTDSWQDEAPTW
SPNGRIIQFFRTEKGSGKASVWQVDLTGRNERRLSTPGDASDPAWGPLLP

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=379, Percent_Identity=28.2321899736148, Blast_Score=146, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB1_NOVAD (Q2GBD2)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_495675.1
- ProteinModelPortal:   Q2GBD2
- SMR:   Q2GBD2
- STRING:   Q2GBD2
- GeneID:   3918277
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_0393
- NMPDR:   fig|48935.1.peg.3750
- eggNOG:   COG0823
- HOGENOM:   HBG644919
- OMA:   SFAPDSY
- PhylomeDB:   Q2GBD2
- ProtClustDB:   PRK05137
- BioCyc:   NARO279238:SARO_0393-MONOMER
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- Gene3D:   G3DSA:3.40.50.10070
- Gene3D:   G3DSA:2.130.10.10
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 48807; Mature: 48676

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRMLFAFALLAAAPAQAQQTEPQPAEEGGLSGTVSDDSEWQDLGIAIPAFATNADVPT
CHHHHHHHHHHHHCCCCHHHCCCCCCHHHCCCEEECCCCCCCCCCCEEEECEECCCCCCC
PTSAGSTSALGQALAQVISANLRNNGLFKPVGPESLPRPGFEQVQAPDFAVWSARSAEML
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCEEEECCCCHHEE
VQGYVRANGDGQLTVGCYLYDVALKQQLEKAGWTVAPADWRRAAHKCADMIYARLSGESP
EEEEEEECCCCEEEEEEEEEHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHCCCCCC
FFDSRVAYIAESGPKDRRMKRLAIMDSDGANHRYLTSGQATALTPRFSPDYKSILYLSYL
CCCCEEEEEECCCCHHHHEEEEEEEECCCCCCEEEECCCCEEECCCCCCCHHEEEEEEEE
NGRPRIYVYDLAANTQRLVTETPGPTFAPRWSPDGKWILYSMATAGNTDIYKVPASGGAS
CCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCC
QRLTDTPGIDVGGSFSPDGSRIVFESDRSGSQQIYVMNADGSDHRRISFFGGRAATPEWS
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCC
PRGDQIAFTHIVGNLRIAVVSPGGGDLRYLTDSWQDEAPTWSPNGRIIQFFRTEKGSGKA
CCCCCEEEEEEECCEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCE
SVWQVDLTGRNERRLSTPGDASDPAWGPLLP
EEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TLRMLFAFALLAAAPAQAQQTEPQPAEEGGLSGTVSDDSEWQDLGIAIPAFATNADVPT
HHHHHHHHHHHHCCCCHHHCCCCCCHHHCCCEEECCCCCCCCCCCEEEECEECCCCCCC
PTSAGSTSALGQALAQVISANLRNNGLFKPVGPESLPRPGFEQVQAPDFAVWSARSAEML
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCEEEECCCCHHEE
VQGYVRANGDGQLTVGCYLYDVALKQQLEKAGWTVAPADWRRAAHKCADMIYARLSGESP
EEEEEEECCCCEEEEEEEEEHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHCCCCCC
FFDSRVAYIAESGPKDRRMKRLAIMDSDGANHRYLTSGQATALTPRFSPDYKSILYLSYL
CCCCEEEEEECCCCHHHHEEEEEEEECCCCCCEEEECCCCEEECCCCCCCHHEEEEEEEE
NGRPRIYVYDLAANTQRLVTETPGPTFAPRWSPDGKWILYSMATAGNTDIYKVPASGGAS
CCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCC
QRLTDTPGIDVGGSFSPDGSRIVFESDRSGSQQIYVMNADGSDHRRISFFGGRAATPEWS
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCC
PRGDQIAFTHIVGNLRIAVVSPGGGDLRYLTDSWQDEAPTWSPNGRIIQFFRTEKGSGKA
CCCCCEEEEEEECCEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCE
SVWQVDLTGRNERRLSTPGDASDPAWGPLLP
EEEEEEECCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA