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The map label for this gene is rpoN2 [H]

Identifier: 87198322

GI number: 87198322

Start: 319545

End: 321041

Strand: Direct

Name: rpoN2 [H]

Synonym: Saro_0297

Alternate gene names: 87198322

Gene position: 319545-321041 (Clockwise)

Preceding gene: 87198321

Following gene: 87198327

Centisome position: 8.97

GC content: 67.94

Gene sequence:

>1497_bases
ATGGCGCTCGGGCCGAGGCTCGATATCCGGCAGACCCAGTCGCTGGTGATGACGCCGCAGCTCCAGCAGGCGATCAAGCT
GCTGGCGTTGTCGAACCTCGAGGTCGAGGCCTTCATCGGCGAGGCGCTGGAGGCCAATCCGCTGCTCGAAATCGGTGAGA
CGGCACCCGCCGAGGCGGTCGACGCCGGCCCCGAAGACCTGCGCCGCACGCATCTCGAATCCTCGCCGGTAGACCAGCTC
GTTGCCGAGGGGCGGGTGGAGGAGGATCGCCCGCTCGACATCGATGTCACTGCGCTCGACCGCGATCGGGACACCGGGGA
TGGCGATTTCGGGGGCGGCACGCTCGAGTTGTCCTCGACCCGCGAAAGTGGCGGCGGCGAGGGGCCGGACATCGACGAAC
GGGGGCGCATCGAGGAAACGCTTGCCGAACATCTCCACGCCCAGATCGGCGCGACGACCTCGGACGCGCAGCTCCTCTTC
GTCGCCCGCTGGCTGATCGACCAGCTCGACGAGGCAGGATATCTCGCCATGCCGATTGGCGAAGTGGCCGAGGCGCTGGG
CCTTTCGCCGCTGGTGGTCGAGCGGGCGCTGGCTCTCGTCCAGTCGCTCGACCCGACCGGGGTCGGCGCACGCAACCTGG
CGGAATGCATCGCGCTCCAGGCCCGGGAGGCGGACCGCTATGATCCGTGCATGGCGCGGCTGATCGACAATCTGGAACTT
GTTGCGCGCGGCGAGATCGCGCGGCTGAAACGCCTGTGCCAGGTCGACGACGAGGACTTTGCCGACATGCTGGCCGAGCT
GCGCGGCTACGACCCGCGCCCCGGCCTGCGCTTCGGCGGGGGCGCGGCGGAGCCGGTCGTGCCCGATATCCTGGTGCGCG
CGGCAAAGGGCGGCTGGGACATCGCGCTCAATCAGGCGACCCTGCCGCGCCTCGTCGTCAATCGCAGCTACTACGTGGAG
ATGCGCGGGGCCTGTGTCGGCAAGGAGGCCAAGGCCTGGCTGGGGGAGAAGCTGGCCGACGCGAACTGGCTGCTGAAGGC
GCTCGACCAGCGGCAGAAGACCATCCTCAAGGTCGCGGCCGAGATCGTGAAGCAGCAGGACGGCTTCTTCCGGCACGGCG
TCGCGCACTTGCGCCCGTTGACGCTGAAGACCGTGGCCGAAGCGATATCGATGCATGAATCGACCGTCAGCCGCGTGACT
TCGAACAAGTACCTCCATTGCGACCGGGGTACCTTCGAGCTGAAGTATTTCTTCACTTCGGGCGTCGGCTCTTCCGACGG
TGAGGGCGCTTCGGCCGCGGCGGTGAAGGCTGCGATCCGCCAGCTCATCGATGCCGAGGACCCCAAGGCAATCCTTTCGG
ACGATGCCCTGGTCGATCTGCTCAAGGCGCGGGGCTTCGACCTTGCCCGGCGCACGGTCGCCAAGTACCGCGAGGCGATC
GGGCTCGGAAGTTCGGTCCAGCGCCGCCGCCAGAAAACACTCGCCGGGGTGCGCTGA

Upstream 100 bases:

>100_bases
GCGCTCGTGGCCGATGCCAACGTGCGGCGGCTCTATCTGGGTGAAGGCTTCACCCTGTGACGATGGCCGGCTGATGCGGA
TCTCGGGGGGGATCTAGGCC

Downstream 100 bases:

>100_bases
CGCCCTGTTGCGACGGATTGCTGGCCTTCACGCCCGACGTCCTTGTACGTGACATTCCCGCCCGGTCGGGCAAGCTCGCA
ATCGCGAAGTAGCGCCTATT

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MALGPRLDIRQTQSLVMTPQLQQAIKLLALSNLEVEAFIGEALEANPLLEIGETAPAEAVDAGPEDLRRTHLESSPVDQL
VAEGRVEEDRPLDIDVTALDRDRDTGDGDFGGGTLELSSTRESGGGEGPDIDERGRIEETLAEHLHAQIGATTSDAQLLF
VARWLIDQLDEAGYLAMPIGEVAEALGLSPLVVERALALVQSLDPTGVGARNLAECIALQAREADRYDPCMARLIDNLEL
VARGEIARLKRLCQVDDEDFADMLAELRGYDPRPGLRFGGGAAEPVVPDILVRAAKGGWDIALNQATLPRLVVNRSYYVE
MRGACVGKEAKAWLGEKLADANWLLKALDQRQKTILKVAAEIVKQQDGFFRHGVAHLRPLTLKTVAEAISMHESTVSRVT
SNKYLHCDRGTFELKYFFTSGVGSSDGEGASAAAVKAAIRQLIDAEDPKAILSDDALVDLLKARGFDLARRTVAKYREAI
GLGSSVQRRRQKTLAGVR

Sequences:

>Translated_498_residues
MALGPRLDIRQTQSLVMTPQLQQAIKLLALSNLEVEAFIGEALEANPLLEIGETAPAEAVDAGPEDLRRTHLESSPVDQL
VAEGRVEEDRPLDIDVTALDRDRDTGDGDFGGGTLELSSTRESGGGEGPDIDERGRIEETLAEHLHAQIGATTSDAQLLF
VARWLIDQLDEAGYLAMPIGEVAEALGLSPLVVERALALVQSLDPTGVGARNLAECIALQAREADRYDPCMARLIDNLEL
VARGEIARLKRLCQVDDEDFADMLAELRGYDPRPGLRFGGGAAEPVVPDILVRAAKGGWDIALNQATLPRLVVNRSYYVE
MRGACVGKEAKAWLGEKLADANWLLKALDQRQKTILKVAAEIVKQQDGFFRHGVAHLRPLTLKTVAEAISMHESTVSRVT
SNKYLHCDRGTFELKYFFTSGVGSSDGEGASAAAVKAAIRQLIDAEDPKAILSDDALVDLLKARGFDLARRTVAKYREAI
GLGSSVQRRRQKTLAGVR
>Mature_497_residues
ALGPRLDIRQTQSLVMTPQLQQAIKLLALSNLEVEAFIGEALEANPLLEIGETAPAEAVDAGPEDLRRTHLESSPVDQLV
AEGRVEEDRPLDIDVTALDRDRDTGDGDFGGGTLELSSTRESGGGEGPDIDERGRIEETLAEHLHAQIGATTSDAQLLFV
ARWLIDQLDEAGYLAMPIGEVAEALGLSPLVVERALALVQSLDPTGVGARNLAECIALQAREADRYDPCMARLIDNLELV
ARGEIARLKRLCQVDDEDFADMLAELRGYDPRPGLRFGGGAAEPVVPDILVRAAKGGWDIALNQATLPRLVVNRSYYVEM
RGACVGKEAKAWLGEKLADANWLLKALDQRQKTILKVAAEIVKQQDGFFRHGVAHLRPLTLKTVAEAISMHESTVSRVTS
NKYLHCDRGTFELKYFFTSGVGSSDGEGASAAAVKAAIRQLIDAEDPKAILSDDALVDLLKARGFDLARRTVAKYREAIG
LGSSVQRRRQKTLAGVR

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=499, Percent_Identity=37.0741482965932, Blast_Score=310, Evalue=1e-85,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 54011; Mature: 53880

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALGPRLDIRQTQSLVMTPQLQQAIKLLALSNLEVEAFIGEALEANPLLEIGETAPAEAV
CCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCHHH
DAGPEDLRRTHLESSPVDQLVAEGRVEEDRPLDIDVTALDRDRDTGDGDFGGGTLELSST
CCCHHHHHHHHCCCCCHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEECCC
RESGGGEGPDIDERGRIEETLAEHLHAQIGATTSDAQLLFVARWLIDQLDEAGYLAMPIG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEECCHH
EVAEALGLSPLVVERALALVQSLDPTGVGARNLAECIALQAREADRYDPCMARLIDNLEL
HHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHH
VARGEIARLKRLCQVDDEDFADMLAELRGYDPRPGLRFGGGAAEPVVPDILVRAAKGGWD
HHCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCE
IALNQATLPRLVVNRSYYVEMRGACVGKEAKAWLGEKLADANWLLKALDQRQKTILKVAA
EEECCCHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
EIVKQQDGFFRHGVAHLRPLTLKTVAEAISMHESTVSRVTSNKYLHCDRGTFELKYFFTS
HHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEEEEC
GVGSSDGEGASAAAVKAAIRQLIDAEDPKAILSDDALVDLLKARGFDLARRTVAKYREAI
CCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
GLGSSVQRRRQKTLAGVR
CCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ALGPRLDIRQTQSLVMTPQLQQAIKLLALSNLEVEAFIGEALEANPLLEIGETAPAEAV
CCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCHHH
DAGPEDLRRTHLESSPVDQLVAEGRVEEDRPLDIDVTALDRDRDTGDGDFGGGTLELSST
CCCHHHHHHHHCCCCCHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEECCC
RESGGGEGPDIDERGRIEETLAEHLHAQIGATTSDAQLLFVARWLIDQLDEAGYLAMPIG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEECCHH
EVAEALGLSPLVVERALALVQSLDPTGVGARNLAECIALQAREADRYDPCMARLIDNLEL
HHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHH
VARGEIARLKRLCQVDDEDFADMLAELRGYDPRPGLRFGGGAAEPVVPDILVRAAKGGWD
HHCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCE
IALNQATLPRLVVNRSYYVEMRGACVGKEAKAWLGEKLADANWLLKALDQRQKTILKVAA
EEECCCHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
EIVKQQDGFFRHGVAHLRPLTLKTVAEAISMHESTVSRVTSNKYLHCDRGTFELKYFFTS
HHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEEEEC
GVGSSDGEGASAAAVKAAIRQLIDAEDPKAILSDDALVDLLKARGFDLARRTVAKYREAI
CCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
GLGSSVQRRRQKTLAGVR
CCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1991712; 12597275 [H]