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The map label for this gene is draG [H]

Identifier: 87198284

GI number: 87198284

Start: 272477

End: 273949

Strand: Direct

Name: draG [H]

Synonym: Saro_0259

Alternate gene names: 87198284

Gene position: 272477-273949 (Clockwise)

Preceding gene: 87198280

Following gene: 87198285

Centisome position: 7.65

GC content: 60.76

Gene sequence:

>1473_bases
ATGAGAACCAGTCAATCCCATCCCCTCCTGATCGCGTCCGTCGAGCCGTTTCCCGGTTTCGGCAGTATCGGTATCACCTT
CTGCCCTGGCAAATTCCAACCGGACGCAATGAGCGGCTCGTGGGCGCGCGATTTAGCCTCCGATCTCGATGCCGTCCGTG
ACTGGGGGGCCACTGCGATCGTAACGCTCGTCACTCAGCGCGAATTGCAGCGTCTCAGCGTGCCAATGCTTGGTGCTGAA
GTAGTAGCGCGCCACATCGACTGGCACCACCTGCCAATCAAGGATTGCGGCACGCCCGATCCCTCGTTCGAAGCTGAGTG
GGAAACCGTTGGCGAAGAGCTTCGCAACCGCCTGCGGAACGGCTTCAACGTGCTGCTGCATTGCAAAGGCGGTTTGGGTC
GAGCTGGTACGGTTGCCGCACGCCTTCTTGTCGAACTTGGGTGGGACGCAAACAAGGCCGTGAGCTCCATTCGCGAGGTT
CGCCCAAACGCCATCGAGACGCTAGATCAGTTCAACCACGTTCTCAGTGCGCGCCACGTGCCAGAATGCCAACCCGACAC
CAGCCTTGATGCCATGCGCGACCGGGCGGTGGGCATTCTGCTCGGTCTCGCAGTTGGTGATGCAGTGGGCACAACTCTCG
AATTTCAGCCCCGCAATGCCTACCCTCGTTTGACAGACATGGTTGGCGGCGGCCCTTTCGCACTTACGCCAGGCGAATGG
ACAGACGACACGTCCATGGCGCTGGCGCTGGGGGACAGCCTTCTGATCTCTAACGGTCTGGACGAGGCGGACTTGATGCG
CCGCTTTGCCGCGTGGCGTGACCAAGGAGAGTACTCGGTCAACGGGCAGTGTTTCGATATCGGCAACACGGTAAGTAGTG
CGCTTCGGAGCTGGAAGCAGAGCGGAAACCCGATTGCTGGTCCAACCGCACCTCATACGGCAGGCAATGGCAGCCTCATG
CGGCTTGGCCCCATCGCAGTTCGCTTCTTCCGGGATCGTGAAGCTATGCGCGATGCAGCCGCGCGCCAAAGCCGCACCAC
TCACGGAGCAAGTGACGCAGTGGATGCCTGCGTAGCGTTTGCCGAAATGCTCGCAAACGCGATCAATGGATGGACTCGAA
CAGAAGTTTTGCAGTCTCCCGACTGGCTCCAATACACGGGTACCATCAATGCGATTGTAGCGGGCTCATGGCGCGGTCTG
CCGCGAAGTAAGGTTCGCGCTAGTGGTTATGTCGCACACTCGCTGGAAGCCTCGCTTTGGAGCGTCGGACGCTCCGGCAC
ATTCGAGCAGGCCGTATTGGCCGCAGCTAACTTGGGCGAAGATGCGGATACCACCGCTGCGATCACTGGACAGCTTGCTG
GCGCGCTCCATGGCGCGGCTGGCATCCCTCGACGGTGGCTCGCCCGGCTCGCATGGAGCCAGCGCATTCGCGAAATGGCC
AATGCTCTCTTCAGCCAAGGCCTGGGTCTGTGA

Upstream 100 bases:

>100_bases
TGCCAATCCGGAAAATCCCGAGCGATGCTCCCAGCAACGTTCGTTGGCTTATCTGCGTCTCACTTGGCGGTCTTGTCCGT
AGAATAGCAAAGGAGACGTC

Downstream 100 bases:

>100_bases
CACTCGTCTTCTCCTCTGAAAGCTCGCGCAAAGGGCATGGATATGTTTGATCATCTCTTCACAGTATGGGGCACCTTGAG
CCTCGACGGACTGATCTTCG

Product: ADP-ribosyl-(dinitrogen reductase) hydrolase

Products: NA

Alternate protein names: ADP-ribosylglycohydrolase; Dinitrogenase reductase-activating glycohydrolase [H]

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MRTSQSHPLLIASVEPFPGFGSIGITFCPGKFQPDAMSGSWARDLASDLDAVRDWGATAIVTLVTQRELQRLSVPMLGAE
VVARHIDWHHLPIKDCGTPDPSFEAEWETVGEELRNRLRNGFNVLLHCKGGLGRAGTVAARLLVELGWDANKAVSSIREV
RPNAIETLDQFNHVLSARHVPECQPDTSLDAMRDRAVGILLGLAVGDAVGTTLEFQPRNAYPRLTDMVGGGPFALTPGEW
TDDTSMALALGDSLLISNGLDEADLMRRFAAWRDQGEYSVNGQCFDIGNTVSSALRSWKQSGNPIAGPTAPHTAGNGSLM
RLGPIAVRFFRDREAMRDAAARQSRTTHGASDAVDACVAFAEMLANAINGWTRTEVLQSPDWLQYTGTINAIVAGSWRGL
PRSKVRASGYVAHSLEASLWSVGRSGTFEQAVLAAANLGEDADTTAAITGQLAGALHGAAGIPRRWLARLAWSQRIREMA
NALFSQGLGL

Sequences:

>Translated_490_residues
MRTSQSHPLLIASVEPFPGFGSIGITFCPGKFQPDAMSGSWARDLASDLDAVRDWGATAIVTLVTQRELQRLSVPMLGAE
VVARHIDWHHLPIKDCGTPDPSFEAEWETVGEELRNRLRNGFNVLLHCKGGLGRAGTVAARLLVELGWDANKAVSSIREV
RPNAIETLDQFNHVLSARHVPECQPDTSLDAMRDRAVGILLGLAVGDAVGTTLEFQPRNAYPRLTDMVGGGPFALTPGEW
TDDTSMALALGDSLLISNGLDEADLMRRFAAWRDQGEYSVNGQCFDIGNTVSSALRSWKQSGNPIAGPTAPHTAGNGSLM
RLGPIAVRFFRDREAMRDAAARQSRTTHGASDAVDACVAFAEMLANAINGWTRTEVLQSPDWLQYTGTINAIVAGSWRGL
PRSKVRASGYVAHSLEASLWSVGRSGTFEQAVLAAANLGEDADTTAAITGQLAGALHGAAGIPRRWLARLAWSQRIREMA
NALFSQGLGL
>Mature_490_residues
MRTSQSHPLLIASVEPFPGFGSIGITFCPGKFQPDAMSGSWARDLASDLDAVRDWGATAIVTLVTQRELQRLSVPMLGAE
VVARHIDWHHLPIKDCGTPDPSFEAEWETVGEELRNRLRNGFNVLLHCKGGLGRAGTVAARLLVELGWDANKAVSSIREV
RPNAIETLDQFNHVLSARHVPECQPDTSLDAMRDRAVGILLGLAVGDAVGTTLEFQPRNAYPRLTDMVGGGPFALTPGEW
TDDTSMALALGDSLLISNGLDEADLMRRFAAWRDQGEYSVNGQCFDIGNTVSSALRSWKQSGNPIAGPTAPHTAGNGSLM
RLGPIAVRFFRDREAMRDAAARQSRTTHGASDAVDACVAFAEMLANAINGWTRTEVLQSPDWLQYTGTINAIVAGSWRGL
PRSKVRASGYVAHSLEASLWSVGRSGTFEQAVLAAANLGEDADTTAAITGQLAGALHGAAGIPRRWLARLAWSQRIREMA
NALFSQGLGL

Specific function: Involved in the regulation of the nitrogen fixation activity by the reversible ADP-ribosylation of the dinitrogenase reductase component of the nitrogenase enzyme complex. The ADP- ribosyltransferase (DraT) transfers the ADP-ribose group from NAD to dinit

COG id: COG1397

COG function: function code O; ADP-ribosylglycohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ADP-ribosylglycohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI195927025, Length=153, Percent_Identity=33.9869281045752, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI17981704, Length=139, Percent_Identity=35.9712230215827, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1788416, Length=307, Percent_Identity=28.0130293159609, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013479
- InterPro:   IPR005502 [H]

Pfam domain/function: PF03747 ADP_ribosyl_GH [H]

EC number: =3.2.2.24 [H]

Molecular weight: Translated: 52685; Mature: 52685

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50056 TYR_PHOSPHATASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSQSHPLLIASVEPFPGFGSIGITFCPGKFQPDAMSGSWARDLASDLDAVRDWGATAI
CCCCCCCCEEEEECCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHH
VTLVTQRELQRLSVPMLGAEVVARHIDWHHLPIKDCGTPDPSFEAEWETVGEELRNRLRN
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHC
GFNVLLHCKGGLGRAGTVAARLLVELGWDANKAVSSIREVRPNAIETLDQFNHVLSARHV
CCEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
PECQPDTSLDAMRDRAVGILLGLAVGDAVGTTLEFQPRNAYPRLTDMVGGGPFALTPGEW
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHCCCCCEEECCCCC
TDDTSMALALGDSLLISNGLDEADLMRRFAAWRDQGEYSVNGQCFDIGNTVSSALRSWKQ
CCCCHHHHHHCCHHHHCCCCCHHHHHHHHHHHHCCCCEECCCEEEECCHHHHHHHHHHHH
SGNPIAGPTAPHTAGNGSLMRLGPIAVRFFRDREAMRDAAARQSRTTHGASDAVDACVAF
CCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
AEMLANAINGWTRTEVLQSPDWLQYTGTINAIVAGSWRGLPRSKVRASGYVAHSLEASLW
HHHHHHHHCCCHHHHHHCCCCCEEECCCEEEEEECCCCCCCHHHHHHCCHHHHHHHHHHH
SVGRSGTFEQAVLAAANLGEDADTTAAITGQLAGALHGAAGIPRRWLARLAWSQRIREMA
HCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
NALFSQGLGL
HHHHHCCCCC
>Mature Secondary Structure
MRTSQSHPLLIASVEPFPGFGSIGITFCPGKFQPDAMSGSWARDLASDLDAVRDWGATAI
CCCCCCCCEEEEECCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHH
VTLVTQRELQRLSVPMLGAEVVARHIDWHHLPIKDCGTPDPSFEAEWETVGEELRNRLRN
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHC
GFNVLLHCKGGLGRAGTVAARLLVELGWDANKAVSSIREVRPNAIETLDQFNHVLSARHV
CCEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
PECQPDTSLDAMRDRAVGILLGLAVGDAVGTTLEFQPRNAYPRLTDMVGGGPFALTPGEW
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHCCCCCEEECCCCC
TDDTSMALALGDSLLISNGLDEADLMRRFAAWRDQGEYSVNGQCFDIGNTVSSALRSWKQ
CCCCHHHHHHCCHHHHCCCCCHHHHHHHHHHHHCCCCEECCCEEEECCHHHHHHHHHHHH
SGNPIAGPTAPHTAGNGSLMRLGPIAVRFFRDREAMRDAAARQSRTTHGASDAVDACVAF
CCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
AEMLANAINGWTRTEVLQSPDWLQYTGTINAIVAGSWRGLPRSKVRASGYVAHSLEASLW
HHHHHHHHCCCHHHHHHCCCCCEEECCCEEEEEECCCCCCCHHHHHHCCHHHHHHHHHHH
SVGRSGTFEQAVLAAANLGEDADTTAAITGQLAGALHGAAGIPRRWLARLAWSQRIREMA
HCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
NALFSQGLGL
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2506427 [H]