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The map label for this gene is 86751695

Identifier: 86751695

GI number: 86751695

Start: 5191304

End: 5192674

Strand: Reverse

Name: 86751695

Synonym: RPB_4597

Alternate gene names: NA

Gene position: 5192674-5191304 (Counterclockwise)

Preceding gene: 86751696

Following gene: 86751694

Centisome position: 97.39

GC content: 65.35

Gene sequence:

>1371_bases
ATGGCGCGTGACCAGATCGATATGACGCCGCTGAACTCGCGCGACGAACTGGTCGCGTGGATCGAAGCGGGCGTCAAACC
GCCGTCGGAATTCCGCATCGGCACCGAACACGAGAAGACGCCGTTCACGCTCGAGGGCCATCACCCGGTACCTTACGACG
GCGCGCGCGGCATCGGCGCGCTGCTCGAGGGCATGAAGATCCTGCTCGGCTGGGAACCGATCATGGAAGGCCCGCACATC
ATCGGGCTGCACGACGTCACCGGCGGCGGCGCGATTTCGCTCGAGCCCGGCGGACAGTTCGAATTGTCCGGCGCGCCGGT
CGACAATGTGCACCAGACCCATTCCGAGCTGATGGCGCATCTGGCGCAGGTGCGCGAGATCGCAGCCCCGCTCGGCATCG
GCTTCCTCGGCCTCGGCATGACGCCGTCGTGGTCGCGCGACGACATTCCGGTGATGCCGAAGGGCCGCTACAAGATCATG
ACCAACTACATGCCGAAGGTCGGCCGCTACGGCCTCGACATGATGTATCGGACCTGCACGGTGCAGACCAATTTGGACTT
CTCGTCCGAAGCCGACATGGTCAAGAAGCTGCGGGTTTCGGTGGCGCTGCAGCCGGTCGCGACCGCTCTGTTCGCCAACT
CGCCGTTCACCGAAGGCAAGCCGAACGGCTTCTTGTCGTTCCGTTCCGAAATCTGGCGCGACACCGACAACGCCCGCTCC
GGCATGATCCCGTGGGCGTTCGAGGACGGCATGGGGTTCGAGCGCTGGGTCGACTACGCGCTCGACGTGCCGATGTATTT
CGTCAAGCGCGGCGATGATTACATCGACGTCTCCGGCTCGTCGTTCCGCGATTTCTTCGACGGCCGAAACGACAAGATGC
CGGGCGAGCGACCGACGCTGTCGGACTGGGCCAACCATCTGTCGACGATCTTCCCCGAAGTGCGGCTGAAGCGTTACCTC
GAAATGCGTGGCGCCGACGGCGTGCCGTGGGGCCGGCTGCCGGCGTTGCCGGCGTTCTGGGTCGGCCTCTTGTACGACGA
CCAGAGCCTCGACGCCGCCTGGGAGATCGTCAAAGGCTGGGACGCCTGGGAGCGGCAGGCGCTGCGCGACGACGTCCCCC
GGCTCGGCTTCAAGGCCAAGATCCGCAACCGATTTCTGTTCGAGATCGCCAAGGAATGCCTGGTGCTGGCCCATGCGGGC
CTGAGGCGCCGCGGCCGGATCGATTCGTTCGGCAACGACGAATCGCGGTATCTCGCGCCGCTCGAGGACATCCTCGCCTC
CGGCCGCACCCCGGCCGAAGAGATGCTGGAGAAATTCAACGGCGCCTGGCAGGGCTCGGTGGAGCCGGCCTACGACGAAT
ACGCGTTCTGA

Upstream 100 bases:

>100_bases
GCGATGATACACAAAACGTCCCTCTCGCTTGCGGCTGTCGTCTGCCGCGACCCCCGCCACCTTGCGCAGACGATCCCCGC
GCGCTGCATCCGGAATTCTC

Downstream 100 bases:

>100_bases
ACCCGACCGGCGAGGGAACGCCGTTCATCGCCCGTACAGGTAGACGACCGTCAGATGCCGTTTTGGTGCGGCGGTCCGGC
TCCATATTCTGGTGCACCGA

Product: glutamate--cysteine ligase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Gamma-Glutamylcysteine Synthetase; Glutamate/Cysteine Ligase Family Protein; Glutamate-Cysteine Ligase GshA; Glutamylcysteine Synthetase; Glutamate-Cysteine Ligase Family 2 Protein; Glutamate-Cysteine Ligase Protein; Glutathione Synthetase; Glutamate-Cysteine Ligase Gsha; Glutamate-Cysteine Ligase Protein; Glutamate-Cysteine Ligase Family Protein; Glutamate Cystein Ligase Protein; Glutamate-Cysteine Ligase GSHA

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MARDQIDMTPLNSRDELVAWIEAGVKPPSEFRIGTEHEKTPFTLEGHHPVPYDGARGIGALLEGMKILLGWEPIMEGPHI
IGLHDVTGGGAISLEPGGQFELSGAPVDNVHQTHSELMAHLAQVREIAAPLGIGFLGLGMTPSWSRDDIPVMPKGRYKIM
TNYMPKVGRYGLDMMYRTCTVQTNLDFSSEADMVKKLRVSVALQPVATALFANSPFTEGKPNGFLSFRSEIWRDTDNARS
GMIPWAFEDGMGFERWVDYALDVPMYFVKRGDDYIDVSGSSFRDFFDGRNDKMPGERPTLSDWANHLSTIFPEVRLKRYL
EMRGADGVPWGRLPALPAFWVGLLYDDQSLDAAWEIVKGWDAWERQALRDDVPRLGFKAKIRNRFLFEIAKECLVLAHAG
LRRRGRIDSFGNDESRYLAPLEDILASGRTPAEEMLEKFNGAWQGSVEPAYDEYAF

Sequences:

>Translated_456_residues
MARDQIDMTPLNSRDELVAWIEAGVKPPSEFRIGTEHEKTPFTLEGHHPVPYDGARGIGALLEGMKILLGWEPIMEGPHI
IGLHDVTGGGAISLEPGGQFELSGAPVDNVHQTHSELMAHLAQVREIAAPLGIGFLGLGMTPSWSRDDIPVMPKGRYKIM
TNYMPKVGRYGLDMMYRTCTVQTNLDFSSEADMVKKLRVSVALQPVATALFANSPFTEGKPNGFLSFRSEIWRDTDNARS
GMIPWAFEDGMGFERWVDYALDVPMYFVKRGDDYIDVSGSSFRDFFDGRNDKMPGERPTLSDWANHLSTIFPEVRLKRYL
EMRGADGVPWGRLPALPAFWVGLLYDDQSLDAAWEIVKGWDAWERQALRDDVPRLGFKAKIRNRFLFEIAKECLVLAHAG
LRRRGRIDSFGNDESRYLAPLEDILASGRTPAEEMLEKFNGAWQGSVEPAYDEYAF
>Mature_455_residues
ARDQIDMTPLNSRDELVAWIEAGVKPPSEFRIGTEHEKTPFTLEGHHPVPYDGARGIGALLEGMKILLGWEPIMEGPHII
GLHDVTGGGAISLEPGGQFELSGAPVDNVHQTHSELMAHLAQVREIAAPLGIGFLGLGMTPSWSRDDIPVMPKGRYKIMT
NYMPKVGRYGLDMMYRTCTVQTNLDFSSEADMVKKLRVSVALQPVATALFANSPFTEGKPNGFLSFRSEIWRDTDNARSG
MIPWAFEDGMGFERWVDYALDVPMYFVKRGDDYIDVSGSSFRDFFDGRNDKMPGERPTLSDWANHLSTIFPEVRLKRYLE
MRGADGVPWGRLPALPAFWVGLLYDDQSLDAAWEIVKGWDAWERQALRDDVPRLGFKAKIRNRFLFEIAKECLVLAHAGL
RRRGRIDSFGNDESRYLAPLEDILASGRTPAEEMLEKFNGAWQGSVEPAYDEYAF

Specific function: Unknown

COG id: COG3572

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 51255; Mature: 51124

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDQIDMTPLNSRDELVAWIEAGVKPPSEFRIGTEHEKTPFTLEGHHPVPYDGARGIGA
CCCCCCCCCCCCCHHHHHHHHHHCCCCCHHCCCCCCCCCCCEEEECCCCCCCCCCCHHHH
LLEGMKILLGWEPIMEGPHIIGLHDVTGGGAISLEPGGQFELSGAPVDNVHQTHSELMAH
HHHHHHHEECCHHHHCCCEEEEEEECCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHH
LAQVREIAAPLGIGFLGLGMTPSWSRDDIPVMPKGRYKIMTNYMPKVGRYGLDMMYRTCT
HHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCEEEHHHHCCHHHHHHHHHHHHEEE
VQTNLDFSSEADMVKKLRVSVALQPVATALFANSPFTEGKPNGFLSFRSEIWRDTDNARS
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCC
GMIPWAFEDGMGFERWVDYALDVPMYFVKRGDDYIDVSGSSFRDFFDGRNDKMPGERPTL
CCCCEEECCCCCHHHHHHHHHCCCHHHHHCCCCEEECCCCHHHHHHCCCCCCCCCCCCCH
SDWANHLSTIFPEVRLKRYLEMRGADGVPWGRLPALPAFWVGLLYDDQSLDAAWEIVKGW
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHCC
DAWERQALRDDVPRLGFKAKIRNRFLFEIAKECLVLAHAGLRRRGRIDSFGNDESRYLAP
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCC
LEDILASGRTPAEEMLEKFNGAWQGSVEPAYDEYAF
HHHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHCCC
>Mature Secondary Structure 
ARDQIDMTPLNSRDELVAWIEAGVKPPSEFRIGTEHEKTPFTLEGHHPVPYDGARGIGA
CCCCCCCCCCCCHHHHHHHHHHCCCCCHHCCCCCCCCCCCEEEECCCCCCCCCCCHHHH
LLEGMKILLGWEPIMEGPHIIGLHDVTGGGAISLEPGGQFELSGAPVDNVHQTHSELMAH
HHHHHHHEECCHHHHCCCEEEEEEECCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHH
LAQVREIAAPLGIGFLGLGMTPSWSRDDIPVMPKGRYKIMTNYMPKVGRYGLDMMYRTCT
HHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCEEEHHHHCCHHHHHHHHHHHHEEE
VQTNLDFSSEADMVKKLRVSVALQPVATALFANSPFTEGKPNGFLSFRSEIWRDTDNARS
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCC
GMIPWAFEDGMGFERWVDYALDVPMYFVKRGDDYIDVSGSSFRDFFDGRNDKMPGERPTL
CCCCEEECCCCCHHHHHHHHHCCCHHHHHCCCCEEECCCCHHHHHHCCCCCCCCCCCCCH
SDWANHLSTIFPEVRLKRYLEMRGADGVPWGRLPALPAFWVGLLYDDQSLDAAWEIVKGW
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHCC
DAWERQALRDDVPRLGFKAKIRNRFLFEIAKECLVLAHAGLRRRGRIDSFGNDESRYLAP
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCC
LEDILASGRTPAEEMLEKFNGAWQGSVEPAYDEYAF
HHHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA