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The map label for this gene is frmA [H]

Identifier: 86750915

GI number: 86750915

Start: 4343970

End: 4345025

Strand: Reverse

Name: frmA [H]

Synonym: RPB_3806

Alternate gene names: 86750915

Gene position: 4345025-4343970 (Counterclockwise)

Preceding gene: 86750916

Following gene: 86750914

Centisome position: 81.49

GC content: 69.13

Gene sequence:

>1056_bases
ATGCCCGTGACCTTCCGCGCCGCCGTTCTTCGTGAGCTGAATCGGCCGCTCGCCATCGAGACCGTCGAGGCGCCGCCGCT
CGCGGCCGGGCAGGTGCTGGTCAAGCTGGCGTATTCCGGGGTGTGCCACAGCCAGGTGATGGAAGCGCGGGGCGGCCGCG
GCGTCGATCGCTATCTGCCGCACATGCTCGGCCACGAGGGCTCGGGCGTCGTGGTCGAGACCGGCGCCGGCGTGACCAAG
GTGAAGACGGGCGATCGCGTCATCCTCGGCTGGATCAAGGGCAAAGGCGCCGACGCGCAGGGCATCCGCTACAAGAGCGG
CGACGGCTTCATCAATGCCGGCGCGGTGACGACGTTCAACGAATACGCCGTGGTCGCGGAGAACCGCGTGACCCTGCTGC
CGCAGGGCCTGCCGATGGACGTCGCGGTGCTGTTCGGCTGCGCGCTGCCGGTCGGCGCCGGCATCGTCATCAATATCGCC
AAGCCCGCGCCCGGCAGCACGCTCGCGGTGTTCGGGCTCGGCGGCATCGGGTTGTCGGCGCTGATGGCGTGCAAGCTGTT
CGACTGCAGGCAACTGATCGCCGTCGATGTCGAGCCCGCGAAGCTGGCGATGGCGCGCGAACTCGGCGCCACCGCGACGA
TCGATGCGTCGCAGCAGGATCCGGTCGCTGCGATCCGGGAGCTGACCGGCGGGCTCGGTGTCGACTACGCCATTGAATCC
GCCGGCCTGGTGCGCGTCATCGAGCAGGCGTTCGACGCCACGCGGCGGTTCGGCGGGCTGTGCGTGTTCGCCTCGCATCC
GCGTTCCGGCGAAAAGATCGCGCTCGACCCGTTCGAACTGATCTGCGGCAAGCGCATCCTCGGCACCTGGGGTGGCGACG
CCAATCCGGACCGCGACGTCGACCTGCTCGCCGGCCTGTTCCGCGCCGGCAAGCTGCCGCTGGCCTCGATGTTCAGCCGC
CGCTACGCGCTCGACGAGATCAACATCGCCCTCGACGATCTCGAACAGCGCCGCAGCGTGCGGCCGCTGATCGAAATCGA
TGCGACATTGGGCTGA

Upstream 100 bases:

>100_bases
CGACGAAGCAATCCAGGGGCGCCGACCGCGGCGCTCTGGATTGCTTCGCTTCGCTCGCAATGACGGGGATGGGTTCGATT
TCCAAAGAGCGGAGTTCGCG

Downstream 100 bases:

>100_bases
CCGCGATGCTGCATCCCGAGACCGAAGCGACGCTGCAGGGGATCCGGGCCGAAGCCGGGCCGGACCGCGCCATCGTGTTC
GTCTCCGGCAATTTCAATAT

Product: alcohol dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MPVTFRAAVLRELNRPLAIETVEAPPLAAGQVLVKLAYSGVCHSQVMEARGGRGVDRYLPHMLGHEGSGVVVETGAGVTK
VKTGDRVILGWIKGKGADAQGIRYKSGDGFINAGAVTTFNEYAVVAENRVTLLPQGLPMDVAVLFGCALPVGAGIVINIA
KPAPGSTLAVFGLGGIGLSALMACKLFDCRQLIAVDVEPAKLAMARELGATATIDASQQDPVAAIRELTGGLGVDYAIES
AGLVRVIEQAFDATRRFGGLCVFASHPRSGEKIALDPFELICGKRILGTWGGDANPDRDVDLLAGLFRAGKLPLASMFSR
RYALDEINIALDDLEQRRSVRPLIEIDATLG

Sequences:

>Translated_351_residues
MPVTFRAAVLRELNRPLAIETVEAPPLAAGQVLVKLAYSGVCHSQVMEARGGRGVDRYLPHMLGHEGSGVVVETGAGVTK
VKTGDRVILGWIKGKGADAQGIRYKSGDGFINAGAVTTFNEYAVVAENRVTLLPQGLPMDVAVLFGCALPVGAGIVINIA
KPAPGSTLAVFGLGGIGLSALMACKLFDCRQLIAVDVEPAKLAMARELGATATIDASQQDPVAAIRELTGGLGVDYAIES
AGLVRVIEQAFDATRRFGGLCVFASHPRSGEKIALDPFELICGKRILGTWGGDANPDRDVDLLAGLFRAGKLPLASMFSR
RYALDEINIALDDLEQRRSVRPLIEIDATLG
>Mature_350_residues
PVTFRAAVLRELNRPLAIETVEAPPLAAGQVLVKLAYSGVCHSQVMEARGGRGVDRYLPHMLGHEGSGVVVETGAGVTKV
KTGDRVILGWIKGKGADAQGIRYKSGDGFINAGAVTTFNEYAVVAENRVTLLPQGLPMDVAVLFGCALPVGAGIVINIAK
PAPGSTLAVFGLGGIGLSALMACKLFDCRQLIAVDVEPAKLAMARELGATATIDASQQDPVAAIRELTGGLGVDYAIESA
GLVRVIEQAFDATRRFGGLCVFASHPRSGEKIALDPFELICGKRILGTWGGDANPDRDVDLLAGLFRAGKLPLASMFSRR
YALDEINIALDDLEQRRSVRPLIEIDATLG

Specific function: Has High Formaldehyde Dehydrogenase Activity In The Presence Of Glutathione And Catalyzes The Oxidation Of Normal Alcohols In A Reaction That Is Not Gsh-Dependent. In Addition, Hemithiolacetals Other Than Those Formed From Gsh, Including Omega-Thiol Fatty

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=371, Percent_Identity=33.9622641509434, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI4501933, Length=369, Percent_Identity=32.2493224932249, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI34577061, Length=369, Percent_Identity=31.7073170731707, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI4501929, Length=372, Percent_Identity=31.989247311828, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI71565152, Length=375, Percent_Identity=30.6666666666667, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI156523966, Length=366, Percent_Identity=30.327868852459, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI4501939, Length=354, Percent_Identity=31.0734463276836, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI262073058, Length=367, Percent_Identity=29.1553133514986, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI71743840, Length=367, Percent_Identity=29.1553133514986, Blast_Score=143, Evalue=2e-34,
Organism=Escherichia coli, GI1786552, Length=367, Percent_Identity=31.8801089918256, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI1788073, Length=265, Percent_Identity=29.0566037735849, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87081918, Length=232, Percent_Identity=31.0344827586207, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788407, Length=320, Percent_Identity=25, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790045, Length=347, Percent_Identity=23.0547550432277, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787863, Length=354, Percent_Identity=27.4011299435028, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI87082125, Length=296, Percent_Identity=28.3783783783784, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25146526, Length=377, Percent_Identity=29.4429708222812, Blast_Score=166, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI71997431, Length=379, Percent_Identity=29.287598944591, Blast_Score=161, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17565904, Length=341, Percent_Identity=29.6187683284457, Blast_Score=138, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17562584, Length=293, Percent_Identity=29.6928327645051, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71988145, Length=288, Percent_Identity=26.7361111111111, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17562582, Length=289, Percent_Identity=28.3737024221453, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6320033, Length=379, Percent_Identity=29.5514511873351, Blast_Score=153, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6319955, Length=358, Percent_Identity=29.3296089385475, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6322619, Length=358, Percent_Identity=29.3296089385475, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6319621, Length=269, Percent_Identity=29.7397769516729, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323961, Length=326, Percent_Identity=27.3006134969325, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324486, Length=329, Percent_Identity=26.4437689969605, Blast_Score=76, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6323729, Length=241, Percent_Identity=25.3112033195021, Blast_Score=63, Evalue=7e-11,
Organism=Drosophila melanogaster, GI17737895, Length=372, Percent_Identity=30.9139784946237, Blast_Score=168, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284; =1.1.1.1 [H]

Molecular weight: Translated: 36942; Mature: 36811

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVTFRAAVLRELNRPLAIETVEAPPLAAGQVLVKLAYSGVCHSQVMEARGGRGVDRYLP
CCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHH
HMLGHEGSGVVVETGAGVTKVKTGDRVILGWIKGKGADAQGIRYKSGDGFINAGAVTTFN
HHHCCCCCCEEEECCCCEEEEECCCEEEEEEEECCCCCCCCCEEECCCCEEECCEEEEEC
EYAVVAENRVTLLPQGLPMDVAVLFGCALPVGAGIVINIAKPAPGSTLAVFGLGGIGLSA
CEEEEECCCEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCHHHHHH
LMACKLFDCRQLIAVDVEPAKLAMARELGATATIDASQQDPVAAIRELTGGLGVDYAIES
HHHHHHHHCCCCEEECCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCCCCHHHHC
AGLVRVIEQAFDATRRFGGLCVFASHPRSGEKIALDPFELICGKRILGTWGGDANPDRDV
CHHHHHHHHHHHHHHHHCCEEEEECCCCCCCEEECCHHHHHHCCCEEECCCCCCCCCCCH
DLLAGLFRAGKLPLASMFSRRYALDEINIALDDLEQRRSVRPLIEIDATLG
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
>Mature Secondary Structure 
PVTFRAAVLRELNRPLAIETVEAPPLAAGQVLVKLAYSGVCHSQVMEARGGRGVDRYLP
CCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHH
HMLGHEGSGVVVETGAGVTKVKTGDRVILGWIKGKGADAQGIRYKSGDGFINAGAVTTFN
HHHCCCCCCEEEECCCCEEEEECCCEEEEEEEECCCCCCCCCEEECCCCEEECCEEEEEC
EYAVVAENRVTLLPQGLPMDVAVLFGCALPVGAGIVINIAKPAPGSTLAVFGLGGIGLSA
CEEEEECCCEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCHHHHHH
LMACKLFDCRQLIAVDVEPAKLAMARELGATATIDASQQDPVAAIRELTGGLGVDYAIES
HHHHHHHHCCCCEEECCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCCCCHHHHC
AGLVRVIEQAFDATRRFGGLCVFASHPRSGEKIALDPFELICGKRILGTWGGDANPDRDV
CHHHHHHHHHHHHHHHHCCEEEEECCCCCCCEEECCHHHHHHCCCEEECCCCCCCCCCCH
DLLAGLFRAGKLPLASMFSRRYALDEINIALDDLEQRRSVRPLIEIDATLG
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]