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The map label for this gene is pyk1 [H]

Identifier: 86750280

GI number: 86750280

Start: 3626139

End: 3627617

Strand: Direct

Name: pyk1 [H]

Synonym: RPB_3169

Alternate gene names: 86750280

Gene position: 3626139-3627617 (Clockwise)

Preceding gene: 86750274

Following gene: 86750281

Centisome position: 68.01

GC content: 69.24

Gene sequence:

>1479_bases
ATGCCGACCGAAGTCGCCCGCCTGAAGCAGAAACTCGACCGGCTGATCGCCGAGATCGAGGCCGACAGCCGGCCGCGCGT
GGCGGCCTGGGGCGACCGGATCGAACGCCCCGGCTTTGCCGCCAGCGCCGGCAACCTGGCGCATTATCTAGCGCTGCGCC
GGCACGATCTGCGACCGCTGCAGCGCGCGCTGATGACGCTCGGACTGTCGTCGCTGGGGCGGCTGGAAAGCCGCGTGATG
CCGACGCTGGTCGCCGTGCGTGCGACCCTGGCGGCGTTGTGCGGCGAGCCGGAGGCGCTGCGGCCGTCGCCGCGGCAATT
CTTCGCGGGCGAGCACGCCCTGGCCGAGCGCAGCGAGGAATTGTTCGGCCTGCCGTCGAGCGGCCGCGCCACCGCGCTGC
TGGTGACCTGCCCGAGCGAGGCCGCCGACGATCCCTCGTTCATGCTGCGGCTGGCGGAGCGCAAGGTCGAGGCGGTGCGG
ATCAACTGCGCCCACGACGATGCCGATGCGTGGCAGCGCATGATCAATCATCTGCATGTCGCCGAGGAAGCCACCGGCCA
CCGCATGAAAGTGCTGATGGACATCGCCGGCCCGAAGATCCGCACCGGCGCCGCGCGCGCACCCGAAGGCCGCGAACGTA
TCCTCACCGGCGACCTGATCGCGATCGTCGCCGCCGGCGCCCTGCACCGCATCGATCTCGCCGACGATCACTTCGCGGTG
GAATGCACCCTGAGCGACCCGCTGATCAACAGCGCCGTCGGCGCGCGGGTGTTCGTCGACGACGGCAAGCTGTGCATCCG
CATCGAGCGCAAGACGCCGTGGGGCCTGGTCGGACGTGTCACCTCGACCTCCGACAAGGGCCTGCGGCTGAAGCCCGAGA
AGGGCCTGAACTTTCCGGACACGCTGCTCGATATCCCGGCCCTGACCGCCAAGGACCGCACCGACCTCGATTTCGTCGCC
GAGCATGCCGACGGCATCGAGTTCTCGTTCGTGCAATCGGTCGCCGACGTCGAGACGCTGCAGACCGCACTGGCCGAGCG
CCGCCCCGACGACTGGCGCAAGCTGTCGCTGGTGTTGAAGATCGAAACGCCCGAAGCGGTCGCCAACCTGCCCGACATTC
TGGTGCAGGCAGCGGGGCAGCAGCCGACCGCGGTGATGATCGCGCGCGGCGACCTCGCGGTGGAGATCGGCTTCGCCCGG
CTCGCCGAGATGCAGGAAGAGATTCTGTGGCTCGGCGAGGCCGCGCACATCCCGGTGATCTGGGCGACGCAGGTGCTGGA
GCAACTCGTCAAGAAGGGCACGCCGTCGCGCGGCGAGATGACCGACGCCGCGATGGCGTCGCGCGCCGAATGCGTGATGC
TCAACAAGGGCCCCTATCTGTTCAAGGCGATCACCGAGCTCGACACCCTGCTCGGCCGGATGGCCGATCACCAGCACAAG
AAGACGCCGCAGCTCCGCAGGCTGCGGAGCTGGGGGTGA

Upstream 100 bases:

>100_bases
CCGGTGACGACAACTCTGTCACAGGAGTTACATCGACGGCCCCTAGACGAACAGATTGGCGGTCGATGGTTCCACCGCCC
GCGATTCAAGAGCCCCCGCC

Downstream 100 bases:

>100_bases
GGACAAGCGGTATCAACCTCAGGTTGACGATCTTGTTCTTATAATGTTCCATCGCCGCCAATTGAGCCCGTTCGCTGCGT
GGGGCGTTCGATGGGATTTC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK 1 [H]

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MPTEVARLKQKLDRLIAEIEADSRPRVAAWGDRIERPGFAASAGNLAHYLALRRHDLRPLQRALMTLGLSSLGRLESRVM
PTLVAVRATLAALCGEPEALRPSPRQFFAGEHALAERSEELFGLPSSGRATALLVTCPSEAADDPSFMLRLAERKVEAVR
INCAHDDADAWQRMINHLHVAEEATGHRMKVLMDIAGPKIRTGAARAPEGRERILTGDLIAIVAAGALHRIDLADDHFAV
ECTLSDPLINSAVGARVFVDDGKLCIRIERKTPWGLVGRVTSTSDKGLRLKPEKGLNFPDTLLDIPALTAKDRTDLDFVA
EHADGIEFSFVQSVADVETLQTALAERRPDDWRKLSLVLKIETPEAVANLPDILVQAAGQQPTAVMIARGDLAVEIGFAR
LAEMQEEILWLGEAAHIPVIWATQVLEQLVKKGTPSRGEMTDAAMASRAECVMLNKGPYLFKAITELDTLLGRMADHQHK
KTPQLRRLRSWG

Sequences:

>Translated_492_residues
MPTEVARLKQKLDRLIAEIEADSRPRVAAWGDRIERPGFAASAGNLAHYLALRRHDLRPLQRALMTLGLSSLGRLESRVM
PTLVAVRATLAALCGEPEALRPSPRQFFAGEHALAERSEELFGLPSSGRATALLVTCPSEAADDPSFMLRLAERKVEAVR
INCAHDDADAWQRMINHLHVAEEATGHRMKVLMDIAGPKIRTGAARAPEGRERILTGDLIAIVAAGALHRIDLADDHFAV
ECTLSDPLINSAVGARVFVDDGKLCIRIERKTPWGLVGRVTSTSDKGLRLKPEKGLNFPDTLLDIPALTAKDRTDLDFVA
EHADGIEFSFVQSVADVETLQTALAERRPDDWRKLSLVLKIETPEAVANLPDILVQAAGQQPTAVMIARGDLAVEIGFAR
LAEMQEEILWLGEAAHIPVIWATQVLEQLVKKGTPSRGEMTDAAMASRAECVMLNKGPYLFKAITELDTLLGRMADHQHK
KTPQLRRLRSWG
>Mature_491_residues
PTEVARLKQKLDRLIAEIEADSRPRVAAWGDRIERPGFAASAGNLAHYLALRRHDLRPLQRALMTLGLSSLGRLESRVMP
TLVAVRATLAALCGEPEALRPSPRQFFAGEHALAERSEELFGLPSSGRATALLVTCPSEAADDPSFMLRLAERKVEAVRI
NCAHDDADAWQRMINHLHVAEEATGHRMKVLMDIAGPKIRTGAARAPEGRERILTGDLIAIVAAGALHRIDLADDHFAVE
CTLSDPLINSAVGARVFVDDGKLCIRIERKTPWGLVGRVTSTSDKGLRLKPEKGLNFPDTLLDIPALTAKDRTDLDFVAE
HADGIEFSFVQSVADVETLQTALAERRPDDWRKLSLVLKIETPEAVANLPDILVQAAGQQPTAVMIARGDLAVEIGFARL
AEMQEEILWLGEAAHIPVIWATQVLEQLVKKGTPSRGEMTDAAMASRAECVMLNKGPYLFKAITELDTLLGRMADHQHKK
TPQLRRLRSWG

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=387, Percent_Identity=27.6485788113695, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI33286422, Length=372, Percent_Identity=27.9569892473118, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI33286420, Length=372, Percent_Identity=27.9569892473118, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI32967597, Length=354, Percent_Identity=27.683615819209, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI10835121, Length=354, Percent_Identity=27.683615819209, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI310128732, Length=313, Percent_Identity=25.5591054313099, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI310128730, Length=313, Percent_Identity=25.5591054313099, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI310128736, Length=257, Percent_Identity=24.5136186770428, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI310128734, Length=257, Percent_Identity=24.5136186770428, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI310128738, Length=222, Percent_Identity=26.5765765765766, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1787965, Length=342, Percent_Identity=26.9005847953216, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788160, Length=331, Percent_Identity=25.9818731117825, Blast_Score=69, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17544584, Length=370, Percent_Identity=27.027027027027, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17506829, Length=365, Percent_Identity=28.7671232876712, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71984413, Length=365, Percent_Identity=28.7671232876712, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17506831, Length=365, Percent_Identity=28.7671232876712, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71984406, Length=365, Percent_Identity=28.7671232876712, Blast_Score=112, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6319279, Length=355, Percent_Identity=28.169014084507, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6324923, Length=360, Percent_Identity=28.6111111111111, Blast_Score=111, Evalue=2e-25,
Organism=Drosophila melanogaster, GI28571814, Length=289, Percent_Identity=30.4498269896194, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24648964, Length=289, Percent_Identity=30.4498269896194, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24648966, Length=325, Percent_Identity=28, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24581235, Length=356, Percent_Identity=25.8426966292135, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 54006; Mature: 53875

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTEVARLKQKLDRLIAEIEADSRPRVAAWGDRIERPGFAASAGNLAHYLALRRHDLRPL
CCHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHH
QRALMTLGLSSLGRLESRVMPTLVAVRATLAALCGEPEALRPSPRQFFAGEHALAERSEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHH
LFGLPSSGRATALLVTCPSEAADDPSFMLRLAERKVEAVRINCAHDDADAWQRMINHLHV
HCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHHHH
AEEATGHRMKVLMDIAGPKIRTGAARAPEGRERILTGDLIAIVAAGALHRIDLADDHFAV
HHHCCCCEEEEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCHHHEEECCCCCEEE
ECTLSDPLINSAVGARVFVDDGKLCIRIERKTPWGLVGRVTSTSDKGLRLKPEKGLNFPD
EEEECCCHHHHHCCCEEEEECCEEEEEEECCCCCCEEEEEECCCCCCEEECCCCCCCCCH
TLLDIPALTAKDRTDLDFVAEHADGIEFSFVQSVADVETLQTALAERRPDDWRKLSLVLK
HHHHCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEE
IETPEAVANLPDILVQAAGQQPTAVMIARGDLAVEIGFARLAEMQEEILWLGEAAHIPVI
EECCHHHHCCHHHHHHHCCCCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCH
WATQVLEQLVKKGTPSRGEMTDAAMASRAECVMLNKGPYLFKAITELDTLLGRMADHQHK
HHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHC
KTPQLRRLRSWG
CCHHHHHHHCCC
>Mature Secondary Structure 
PTEVARLKQKLDRLIAEIEADSRPRVAAWGDRIERPGFAASAGNLAHYLALRRHDLRPL
CHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHH
QRALMTLGLSSLGRLESRVMPTLVAVRATLAALCGEPEALRPSPRQFFAGEHALAERSEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHH
LFGLPSSGRATALLVTCPSEAADDPSFMLRLAERKVEAVRINCAHDDADAWQRMINHLHV
HCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHHHH
AEEATGHRMKVLMDIAGPKIRTGAARAPEGRERILTGDLIAIVAAGALHRIDLADDHFAV
HHHCCCCEEEEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCHHHEEECCCCCEEE
ECTLSDPLINSAVGARVFVDDGKLCIRIERKTPWGLVGRVTSTSDKGLRLKPEKGLNFPD
EEEECCCHHHHHCCCEEEEECCEEEEEEECCCCCCEEEEEECCCCCCEEECCCCCCCCCH
TLLDIPALTAKDRTDLDFVAEHADGIEFSFVQSVADVETLQTALAERRPDDWRKLSLVLK
HHHHCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEE
IETPEAVANLPDILVQAAGQQPTAVMIARGDLAVEIGFARLAEMQEEILWLGEAAHIPVI
EECCHHHHCCHHHHHHHCCCCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCH
WATQVLEQLVKKGTPSRGEMTDAAMASRAECVMLNKGPYLFKAITELDTLLGRMADHQHK
HHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHC
KTPQLRRLRSWG
CCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]