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The map label for this gene is apbE [H]

Identifier: 86750258

GI number: 86750258

Start: 3599915

End: 3600949

Strand: Direct

Name: apbE [H]

Synonym: RPB_3147

Alternate gene names: 86750258

Gene position: 3599915-3600949 (Clockwise)

Preceding gene: 86750257

Following gene: 86750261

Centisome position: 67.52

GC content: 69.86

Gene sequence:

>1035_bases
ATGATGCCCCGCTCCCTCACGCGCCGTCGTTTGATCACCATCGCCGCCGCGGCTGCCGCAGGATCGCTGTGGCCCGGCCG
ATCACGCGCGGCAGCAGGGCCCGAGCCGGTGCGCTGGCAAGGCGCCGCGCTCGGCGCGCAGGTGTCGATCGAGATTCACC
ATCCGGATCGCGCCGCCGCCGCGCGACTGGTTGAGCGCTCGATCGCCGAAGTGCGGCGGCTGGAGCGGCAGTTCAGCCTG
TATCAGCCGGACTCCGCGATCTGCGAACTTAACCGCAGCGGCGTGCTGATTGCGCCTGATCCCGACATGGTGACGCTGCT
GCAGGCCTCGCTCGGCTATGCCGATCTGACCGGCGGCGCGTTCGATCCGACGGTGCAGCCGTTGTGGCGCCTGTATCAGC
AGCACTTCTCATCCGACCGGACCGATCCCGCAGGCCCCTCCTCGGCATGGCTCGAACAGGCGCTGGAGAAGGTCGGATAT
GATGGACTGCGCGTCACGCCCGACCGCATCGTGTTGCTCAAGCGCGGCGCCGCGATCACGCTGAACGGCATCGCCCAAGG
TTATGCGACCGATCGCGTCGTCGAATTGCTCCGGAATGCGGGGCTGTCGACGACGCTGGTCGATATCGGCGAAGTCCGCG
CGCTCGGCGGGCGGCCGGACGGCACGCCCTGGCGCGTCGGCCTCGCCGATCCGGACCAGCCCGGCCGATCCGGCGAGATC
GTCGAAATCGCCGACCGGGCCGTGGCGACGTCTGCGGGCGCCGGCTTCCGGTTCGATCCGGCGGGCCGCTTCACCCATTT
GCTCGACCCGCGGACCGGCCGCAGCCCGCGCTCGTACAATTCGGTCAGCGTCATCGCGCCGACGGCGACCGCAGCGGACG
CGCTGTCGACCGGCTTCAGCCTGATGCCGCTGCCGATGATCCAGCGCATCGTCGATCAATCCCATGGCGTGGAGGCGCGC
ATTCTCGACCTCGCCGGTCAGAGGCTCCACCTCCAGGCGGCGTCCGGACGGAGGGGACACCGCTCCGCGTCGTGA

Upstream 100 bases:

>100_bases
CGACCAGATTCCCGGCGACTACGTCCTCGGCAGCGAGCCATCCGGCACCGCAGGTACCCCGGACGATTCCGGCGTGCGCA
CCAACTAACGAGGACCGCCG

Downstream 100 bases:

>100_bases
CGACGCCTCGATTCCGCGATCATCGCGGCCGCCACGGCGGCATCGGCCCATTCAGGACGACGAACAATGCGAAGGCCGCC
GCCGACGGTCTCCCTTACGA

Product: twin-arginine translocation pathway signal

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MMPRSLTRRRLITIAAAAAAGSLWPGRSRAAAGPEPVRWQGAALGAQVSIEIHHPDRAAAARLVERSIAEVRRLERQFSL
YQPDSAICELNRSGVLIAPDPDMVTLLQASLGYADLTGGAFDPTVQPLWRLYQQHFSSDRTDPAGPSSAWLEQALEKVGY
DGLRVTPDRIVLLKRGAAITLNGIAQGYATDRVVELLRNAGLSTTLVDIGEVRALGGRPDGTPWRVGLADPDQPGRSGEI
VEIADRAVATSAGAGFRFDPAGRFTHLLDPRTGRSPRSYNSVSVIAPTATAADALSTGFSLMPLPMIQRIVDQSHGVEAR
ILDLAGQRLHLQAASGRRGHRSAS

Sequences:

>Translated_344_residues
MMPRSLTRRRLITIAAAAAAGSLWPGRSRAAAGPEPVRWQGAALGAQVSIEIHHPDRAAAARLVERSIAEVRRLERQFSL
YQPDSAICELNRSGVLIAPDPDMVTLLQASLGYADLTGGAFDPTVQPLWRLYQQHFSSDRTDPAGPSSAWLEQALEKVGY
DGLRVTPDRIVLLKRGAAITLNGIAQGYATDRVVELLRNAGLSTTLVDIGEVRALGGRPDGTPWRVGLADPDQPGRSGEI
VEIADRAVATSAGAGFRFDPAGRFTHLLDPRTGRSPRSYNSVSVIAPTATAADALSTGFSLMPLPMIQRIVDQSHGVEAR
ILDLAGQRLHLQAASGRRGHRSAS
>Mature_344_residues
MMPRSLTRRRLITIAAAAAAGSLWPGRSRAAAGPEPVRWQGAALGAQVSIEIHHPDRAAAARLVERSIAEVRRLERQFSL
YQPDSAICELNRSGVLIAPDPDMVTLLQASLGYADLTGGAFDPTVQPLWRLYQQHFSSDRTDPAGPSSAWLEQALEKVGY
DGLRVTPDRIVLLKRGAAITLNGIAQGYATDRVVELLRNAGLSTTLVDIGEVRALGGRPDGTPWRVGLADPDQPGRSGEI
VEIADRAVATSAGAGFRFDPAGRFTHLLDPRTGRSPRSYNSVSVIAPTATAADALSTGFSLMPLPMIQRIVDQSHGVEAR
ILDLAGQRLHLQAASGRRGHRSAS

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=313, Percent_Identity=29.3929712460064, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 36821; Mature: 36821

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPRSLTRRRLITIAAAAAAGSLWPGRSRAAAGPEPVRWQGAALGAQVSIEIHHPDRAAA
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCEECCEEEEEEEECCCHHHH
ARLVERSIAEVRRLERQFSLYQPDSAICELNRSGVLIAPDPDMVTLLQASLGYADLTGGA
HHHHHHHHHHHHHHHHHHCCCCCCHHHHEECCCCEEECCCCCHHHHHHHHCCCEECCCCC
FDPTVQPLWRLYQQHFSSDRTDPAGPSSAWLEQALEKVGYDGLRVTPDRIVLLKRGAAIT
CCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCEEEEEECCCEEE
LNGIAQGYATDRVVELLRNAGLSTTLVDIGEVRALGGRPDGTPWRVGLADPDQPGRSGEI
ECCHHCCHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCE
VEIADRAVATSAGAGFRFDPAGRFTHLLDPRTGRSPRSYNSVSVIAPTATAADALSTGFS
EEHHHHHHHHCCCCCCEECCCCCEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHCCCC
LMPLPMIQRIVDQSHGVEARILDLAGQRLHLQAASGRRGHRSAS
CCCHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MMPRSLTRRRLITIAAAAAAGSLWPGRSRAAAGPEPVRWQGAALGAQVSIEIHHPDRAAA
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCEECCEEEEEEEECCCHHHH
ARLVERSIAEVRRLERQFSLYQPDSAICELNRSGVLIAPDPDMVTLLQASLGYADLTGGA
HHHHHHHHHHHHHHHHHHCCCCCCHHHHEECCCCEEECCCCCHHHHHHHHCCCEECCCCC
FDPTVQPLWRLYQQHFSSDRTDPAGPSSAWLEQALEKVGYDGLRVTPDRIVLLKRGAAIT
CCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCEEEEEECCCEEE
LNGIAQGYATDRVVELLRNAGLSTTLVDIGEVRALGGRPDGTPWRVGLADPDQPGRSGEI
ECCHHCCHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCE
VEIADRAVATSAGAGFRFDPAGRFTHLLDPRTGRSPRSYNSVSVIAPTATAADALSTGFS
EEHHHHHHHHCCCCCCEECCCCCEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHCCCC
LMPLPMIQRIVDQSHGVEARILDLAGQRLHLQAASGRRGHRSAS
CCCHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]