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The map label for this gene is gyrB [H]

Identifier: 86750095

GI number: 86750095

Start: 3389820

End: 3391874

Strand: Direct

Name: gyrB [H]

Synonym: RPB_2978

Alternate gene names: 86750095

Gene position: 3389820-3391874 (Clockwise)

Preceding gene: 86750092

Following gene: 86750096

Centisome position: 63.58

GC content: 66.37

Gene sequence:

>2055_bases
ATGGCCAAGGCATTGAAGGCGAGCAGCAAATCGAAACCGGCCTCCGACCTGTTCGGAGCCGCGGCGAAATCCGGTGCGCC
GCGCGCGGCCGCCAAGCCGACCGGCGGCGCCGAGGCCGGTTACACCGCCGCCGACATCGAGGTGCTCGAAGGGCTGGAGC
CGGTGCGGCGCCGCCCCGGCATGTATATCGGCGGCACCGACGAGAAGGCGCTGCATCACCTGTTCGCCGAAGTGATCGAC
AACTCGATGGACGAGGCGCTGGCCGGCCACGCCACCTTCATCGAGGTCGAGCTCGGCGCCGACGGCTACCTCACCGTCTC
CGACAACGGCCGCGGCATCCCCGTTGATCCGCATCCGAAATTTCCGAAAAAGTCGGCGCTCGAAGTGATCATGTGCACGC
TGCATGCCGGCGGCAAATTCGACAGCAAGGTGTACGAGACCTCGGGCGGTCTGCACGGCGTCGGCGTCAGCGTCGTCAAT
GCGTTGTCGTCGCTGCTTGAAGTGGAAGTCGCGCGCGGCCAGCAACTCTACCGCATGGTGTTCGCGCGCGGCATCCCGCA
GGGCAAGCTGGAGAGCCTGGGCAAGATCAACAACCGCCGCGGCACCCGCGTCCGCTTCAAGCCCGACACCGACATCTTCG
GCGCCAAAGCGGCGTTCAAGCCGCAGCGGCTGTTCAAGATGGCGCGGTCGAAGGCGTATCTGTTCGGCGGCGTCGAGATC
CGCTGGAAGTGCGACCCCGAATTGCTGAAGGGTGTCGAGGATGTTCCGCCCGAGGACACCTTCCACTTCCCCGGCGGCCT
GAAGGACTATCTCGGCGCGGCGATCCACGCCGACACGCTGGTGCATCCCGACATTTTCTCCGGCAAGTCCGGCCGCAACG
GCGCCCACGGCGCCTGCGAATGGGCCGTCGCCTGGACCGCGGACGCCGACGGCTTCCTGTCGTCCTACTGCAACACGGTG
CCGACGCCCGACGGCGGCACCCATGAATCCGGCATGCGCTCCGCTTTGCTGCGCGGCCTCAAGGATCACGCCGAGCGCGT
CGGCCAGGGCAAGCGCGCCTCGTCGATCACCTCCGAAGACGTCATGGTCGGCGCCGCGGTGATGCTCAGCGTGTTCGTGC
GCGAGCCGGAATTCCAGGGCCAGACCAAGGACCGCCTCGCCACCGCCGAAGCGCAGAAGATCGTCGAACAGGCGATCAAG
GACCCGTTCGACCATTGGCTGTCCGGCAACCCGGTGCAGGCCAACAAGCTCCTGGACTTCGTCGTCGAGCGCGCCGAGGA
GCGGCTGCGCCGCCGCCAGGAAAAGGAAATCTCGCGCAAGACCGCGGTGAAGAAGCTGCGGCTGCCCGGCAAGCTCGCCG
ACTGCACCAACACCGCCGCCGAAGGCTCCGAACTGTTCATCGTCGAGGGCGATTCGGCCGGCGGCAGCGCCAAGCAGGCG
CGCGACCGCAAGACGCAAGCTGTGCTCCCCTTGCGCGGCAAAATCCTCAACGTCGCCTCGGCGACCAAGGACAAGGTGGT
GGCGAACGCCCAGCTCTCCGACCTGATGCAGGCGATCGGCTGCGGCACCGGCGTGAACTACCGCGAGGAAGATCTGCGCT
ACGCGCGCATCATCATCATGACCGACGCCGACGTCGACGGCGCGCATATCGCCTCGCTGCTGATCACCTTCTTCTATCGG
CAGATGCCGAAGCTGATCGACGAAGGCCACCTCTATCTCGCGGTGCCGCCGCTGTACCGCCTGACCCATGGCGCCAAGAC
CGTCTACGCCCGCGACGACGCCCACAAGGACGAGCTGCTGAAGAAGGAATTCAACGCCAACGCCAAGGTCGATGTCGGCC
GCTTCAAAGGCCTCGGCGAGATGATGCCCGCCCAGCTCAAGGAAACCACCATGGACCCGGCGCGCCGCACGTTGCTGCGC
GTCAGCCTGCTGGACATCGACCGCGACGGCACCGCCGATTCCGTCGAGCGCCTGATGGGCACCAAGGCCGAAGCAAGGTT
CGCCTTCATCAGCGACAAGGCGGAATTCGCGAGCGAGGATCTGCTGGACGTGTGA

Upstream 100 bases:

>100_bases
GCGCTTCCATGGCATAGTTGACCGCACCGATTCGGCGGGCGCGCGGCGCCGGCGGCAGGCAATCCGATCTCGAGACGACT
CCAGCACAGCTTCAGGACTC

Downstream 100 bases:

>100_bases
ACCCGATACGAAAGCGCTGCGCATGCTTACGCATTTAAAACTACAAAACTTCAAATCTTGGAGAAATGCCAATATCGCAC
TCGCGCCACTTACAGCCCTC

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 684; Mature: 683

Protein sequence:

>684_residues
MAKALKASSKSKPASDLFGAAAKSGAPRAAAKPTGGAEAGYTAADIEVLEGLEPVRRRPGMYIGGTDEKALHHLFAEVID
NSMDEALAGHATFIEVELGADGYLTVSDNGRGIPVDPHPKFPKKSALEVIMCTLHAGGKFDSKVYETSGGLHGVGVSVVN
ALSSLLEVEVARGQQLYRMVFARGIPQGKLESLGKINNRRGTRVRFKPDTDIFGAKAAFKPQRLFKMARSKAYLFGGVEI
RWKCDPELLKGVEDVPPEDTFHFPGGLKDYLGAAIHADTLVHPDIFSGKSGRNGAHGACEWAVAWTADADGFLSSYCNTV
PTPDGGTHESGMRSALLRGLKDHAERVGQGKRASSITSEDVMVGAAVMLSVFVREPEFQGQTKDRLATAEAQKIVEQAIK
DPFDHWLSGNPVQANKLLDFVVERAEERLRRRQEKEISRKTAVKKLRLPGKLADCTNTAAEGSELFIVEGDSAGGSAKQA
RDRKTQAVLPLRGKILNVASATKDKVVANAQLSDLMQAIGCGTGVNYREEDLRYARIIIMTDADVDGAHIASLLITFFYR
QMPKLIDEGHLYLAVPPLYRLTHGAKTVYARDDAHKDELLKKEFNANAKVDVGRFKGLGEMMPAQLKETTMDPARRTLLR
VSLLDIDRDGTADSVERLMGTKAEARFAFISDKAEFASEDLLDV

Sequences:

>Translated_684_residues
MAKALKASSKSKPASDLFGAAAKSGAPRAAAKPTGGAEAGYTAADIEVLEGLEPVRRRPGMYIGGTDEKALHHLFAEVID
NSMDEALAGHATFIEVELGADGYLTVSDNGRGIPVDPHPKFPKKSALEVIMCTLHAGGKFDSKVYETSGGLHGVGVSVVN
ALSSLLEVEVARGQQLYRMVFARGIPQGKLESLGKINNRRGTRVRFKPDTDIFGAKAAFKPQRLFKMARSKAYLFGGVEI
RWKCDPELLKGVEDVPPEDTFHFPGGLKDYLGAAIHADTLVHPDIFSGKSGRNGAHGACEWAVAWTADADGFLSSYCNTV
PTPDGGTHESGMRSALLRGLKDHAERVGQGKRASSITSEDVMVGAAVMLSVFVREPEFQGQTKDRLATAEAQKIVEQAIK
DPFDHWLSGNPVQANKLLDFVVERAEERLRRRQEKEISRKTAVKKLRLPGKLADCTNTAAEGSELFIVEGDSAGGSAKQA
RDRKTQAVLPLRGKILNVASATKDKVVANAQLSDLMQAIGCGTGVNYREEDLRYARIIIMTDADVDGAHIASLLITFFYR
QMPKLIDEGHLYLAVPPLYRLTHGAKTVYARDDAHKDELLKKEFNANAKVDVGRFKGLGEMMPAQLKETTMDPARRTLLR
VSLLDIDRDGTADSVERLMGTKAEARFAFISDKAEFASEDLLDV
>Mature_683_residues
AKALKASSKSKPASDLFGAAAKSGAPRAAAKPTGGAEAGYTAADIEVLEGLEPVRRRPGMYIGGTDEKALHHLFAEVIDN
SMDEALAGHATFIEVELGADGYLTVSDNGRGIPVDPHPKFPKKSALEVIMCTLHAGGKFDSKVYETSGGLHGVGVSVVNA
LSSLLEVEVARGQQLYRMVFARGIPQGKLESLGKINNRRGTRVRFKPDTDIFGAKAAFKPQRLFKMARSKAYLFGGVEIR
WKCDPELLKGVEDVPPEDTFHFPGGLKDYLGAAIHADTLVHPDIFSGKSGRNGAHGACEWAVAWTADADGFLSSYCNTVP
TPDGGTHESGMRSALLRGLKDHAERVGQGKRASSITSEDVMVGAAVMLSVFVREPEFQGQTKDRLATAEAQKIVEQAIKD
PFDHWLSGNPVQANKLLDFVVERAEERLRRRQEKEISRKTAVKKLRLPGKLADCTNTAAEGSELFIVEGDSAGGSAKQAR
DRKTQAVLPLRGKILNVASATKDKVVANAQLSDLMQAIGCGTGVNYREEDLRYARIIIMTDADVDGAHIASLLITFFYRQ
MPKLIDEGHLYLAVPPLYRLTHGAKTVYARDDAHKDELLKKEFNANAKVDVGRFKGLGEMMPAQLKETTMDPARRTLLRV
SLLDIDRDGTADSVERLMGTKAEARFAFISDKAEFASEDLLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=632, Percent_Identity=24.2088607594937, Blast_Score=143, Evalue=6e-34,
Organism=Homo sapiens, GI19913408, Length=637, Percent_Identity=24.8037676609105, Blast_Score=135, Evalue=1e-31,
Organism=Escherichia coli, GI1789408, Length=640, Percent_Identity=42.5, Blast_Score=462, Evalue=1e-131,
Organism=Escherichia coli, GI48994957, Length=565, Percent_Identity=44.6017699115044, Blast_Score=449, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI17535065, Length=640, Percent_Identity=22.8125, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI212645845, Length=577, Percent_Identity=22.5303292894281, Blast_Score=111, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI212645657, Length=209, Percent_Identity=27.2727272727273, Blast_Score=73, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=670, Percent_Identity=22.9850746268657, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI17136538, Length=613, Percent_Identity=24.1435562805873, Blast_Score=123, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 74245; Mature: 74114

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKALKASSKSKPASDLFGAAAKSGAPRAAAKPTGGAEAGYTAADIEVLEGLEPVRRRPG
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHCCC
MYIGGTDEKALHHLFAEVIDNSMDEALAGHATFIEVELGADGYLTVSDNGRGIPVDPHPK
EEECCCCHHHHHHHHHHHHHCHHHHHHCCCEEEEEEEECCCEEEEECCCCCCCCCCCCCC
FPKKSALEVIMCTLHAGGKFDSKVYETSGGLHGVGVSVVNALSSLLEVEVARGQQLYRMV
CCCHHHHHHHHEECCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FARGIPQGKLESLGKINNRRGTRVRFKPDTDIFGAKAAFKPQRLFKMARSKAYLFGGVEI
HHCCCCCHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCEEEEECEEE
RWKCDPELLKGVEDVPPEDTFHFPGGLKDYLGAAIHADTLVHPDIFSGKSGRNGAHGACE
EEECCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEE
WAVAWTADADGFLSSYCNTVPTPDGGTHESGMRSALLRGLKDHAERVGQGKRASSITSED
EEEEEECCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
VMVGAAVMLSVFVREPEFQGQTKDRLATAEAQKIVEQAIKDPFDHWLSGNPVQANKLLDF
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCHHHHHHH
VVERAEERLRRRQEKEISRKTAVKKLRLPGKLADCTNTAAEGSELFIVEGDSAGGSAKQA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCEEEEEECCCCCCCHHHH
RDRKTQAVLPLRGKILNVASATKDKVVANAQLSDLMQAIGCGTGVNYREEDLRYARIIIM
HHHHHHEECCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCEEEEEEE
TDADVDGAHIASLLITFFYRQMPKLIDEGHLYLAVPPLYRLTHGAKTVYARDDAHKDELL
ECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHCCCCEEEECCCCHHHHHH
KKEFNANAKVDVGRFKGLGEMMPAQLKETTMDPARRTLLRVSLLDIDRDGTADSVERLMG
HHHHCCCCEEEHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHEECCCCCCHHHHHHHHC
TKAEARFAFISDKAEFASEDLLDV
CCCCCEEEEECCHHHHHHHHHCCC
>Mature Secondary Structure 
AKALKASSKSKPASDLFGAAAKSGAPRAAAKPTGGAEAGYTAADIEVLEGLEPVRRRPG
CCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHCCC
MYIGGTDEKALHHLFAEVIDNSMDEALAGHATFIEVELGADGYLTVSDNGRGIPVDPHPK
EEECCCCHHHHHHHHHHHHHCHHHHHHCCCEEEEEEEECCCEEEEECCCCCCCCCCCCCC
FPKKSALEVIMCTLHAGGKFDSKVYETSGGLHGVGVSVVNALSSLLEVEVARGQQLYRMV
CCCHHHHHHHHEECCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FARGIPQGKLESLGKINNRRGTRVRFKPDTDIFGAKAAFKPQRLFKMARSKAYLFGGVEI
HHCCCCCHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCEEEEECEEE
RWKCDPELLKGVEDVPPEDTFHFPGGLKDYLGAAIHADTLVHPDIFSGKSGRNGAHGACE
EEECCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEE
WAVAWTADADGFLSSYCNTVPTPDGGTHESGMRSALLRGLKDHAERVGQGKRASSITSED
EEEEEECCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
VMVGAAVMLSVFVREPEFQGQTKDRLATAEAQKIVEQAIKDPFDHWLSGNPVQANKLLDF
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCHHHHHHH
VVERAEERLRRRQEKEISRKTAVKKLRLPGKLADCTNTAAEGSELFIVEGDSAGGSAKQA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCEEEEEECCCCCCCHHHH
RDRKTQAVLPLRGKILNVASATKDKVVANAQLSDLMQAIGCGTGVNYREEDLRYARIIIM
HHHHHHEECCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCEEEEEEE
TDADVDGAHIASLLITFFYRQMPKLIDEGHLYLAVPPLYRLTHGAKTVYARDDAHKDELL
ECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHCCCCEEEECCCCHHHHHH
KKEFNANAKVDVGRFKGLGEMMPAQLKETTMDPARRTLLRVSLLDIDRDGTADSVERLMG
HHHHCCCCEEEHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHEECCCCCCHHHHHHHHC
TKAEARFAFISDKAEFASEDLLDV
CCCCCEEEEECCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9797224 [H]