Click here to switch to the map view.

The map label for this gene is gltX

Identifier: 86749929

GI number: 86749929

Start: 3204605

End: 3206026

Strand: Direct

Name: gltX

Synonym: RPB_2812

Alternate gene names: 86749929

Gene position: 3204605-3206026 (Clockwise)

Preceding gene: 86749926

Following gene: 86749930

Centisome position: 60.11

GC content: 67.79

Gene sequence:

>1422_bases
ATGACCCGACCCGTCGTCACCCGCTTTGCGCCCTCGCCCACCGGCTTCCTCCATATCGGCGGCGGCCGCACCGCTTTGTT
CAACTGGCTTTACGCCCGCAAGCTCGGCGGCAAGATGCTGCTGCGAATCGAAGACACCGATCGCCAGCGCTCCACCCAGC
CGGCGATCGACGCCATCCTCGACGGGCTGAAATGGCTCGGCATCGAATGGGACGGCGACACCGTCTACCAGTTCGCCCGG
GCCGCGCGGCATCGCGAGGTCGCCGAGCAATTGCTGGCGGCCGGCAAGGCCTATCGCTGCTACGCGACCGCCGAGGAACT
GACCGCGATGCGCGACAAGGCCCGCGCCGAGGGCCGCTCGAAACTCTACGACGGCAGCTGGCGCGACCGCGATCCGTCGG
AGGCGCCGGCGGACATGAAGCCGACGATCCGGCTCAAGGCGCCGCTCGACGGCGAGACCGTGATCGAGGATCAGGTCCAG
GGACGCGTCGTCTGGCAGAACGAAAATCTCGACGACCTGGTGCTGCTCCGCGGCGACGGCACGCCGACCTACATGCTCGC
TGTGGTGGTCGACGATCACGACATGGACGTCACCCACGTCATCCGCGGCGACGACCATCTCATCAATGCGGCGCGGCAGA
AGCAGATCTACGACGCGATGGGATGGGAGCTGCCGGTAATGGCACACATCCCGCTCATCCACGGCCCCGACGGTTCGAAG
CTCTCCAAGCGCCACGGCGCGCTCGGTGTCGACGCCTATCGCGCGATGGGCTACCTGCCGGCGGCGCTGCGCAACTATCT
GGTCCGGCTCGGCTGGAGCCACGGCGATCAGGAAATCTTTACCACGCAGGAAATGATCGATGCGTTCGACCTGGCCGCGA
TCGGCCGCTCCGCGGCGCGGTTCGATTTCGCCAAGCTGGAAAGCCTCAATGGCCACTACATCCGCCAGAGCGACGATCAA
TCTCTCGTGACCCTGCTGGAGGATCTGCTGCATTACGTCCCGCAGGGACCGGCGATCGCCGCCCGGCTCACCGACACCAC
CCGTGCCCAGCTGGTGCAGGCGATGCCGGGGCTGAAAGAGCGGGCCAAGACGCTGCTCGAACTGCTCGACAATGCCGGCT
TCATCTTCGCCGACCGTCCGCTCGCGATCGACGCCAAGGGCCAGGCCGTGCTGACGCCCGACATTCGCGCCCTGATCGGG
CGCCTGCGCACCGCGCTGGAGGACGTCTCGCCGTGGACCGCGGCGACCACCGAAGCGGCGATGCGGACGTTCGCGGAACA
GGCCGGGCTCAAGCTCGGCGCGGTTGCGCAACCGCTGCGCGTCGCGCTGACCGGACGGACCACGTCACCGGGGATTTTCG
ACGTTCTGGCCGTGCTGGGCCGCGAGGAGTGCCTGTCCCGCCTCGCCGATCAGGCCGCGTAG

Upstream 100 bases:

>100_bases
CGCGCCAACCCCCGAAGGCTGCGCGGACCACTTTAGTTATGCGCCGATCCTGCTATACACCCTCCGCGCCGCTCCGGCGC
GCTTCCAATCCCAGTTGAGA

Downstream 100 bases:

>100_bases
CCCGCCCCGGGATCGGCCCGGGACAGGCCGTCTTGCGGTGCACACTGCAATAAGCTACGCCTTTCCAGTGTGCTTTTAGG
ACAACCAGTCTCGCTCGCAT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MTRPVVTRFAPSPTGFLHIGGGRTALFNWLYARKLGGKMLLRIEDTDRQRSTQPAIDAILDGLKWLGIEWDGDTVYQFAR
AARHREVAEQLLAAGKAYRCYATAEELTAMRDKARAEGRSKLYDGSWRDRDPSEAPADMKPTIRLKAPLDGETVIEDQVQ
GRVVWQNENLDDLVLLRGDGTPTYMLAVVVDDHDMDVTHVIRGDDHLINAARQKQIYDAMGWELPVMAHIPLIHGPDGSK
LSKRHGALGVDAYRAMGYLPAALRNYLVRLGWSHGDQEIFTTQEMIDAFDLAAIGRSAARFDFAKLESLNGHYIRQSDDQ
SLVTLLEDLLHYVPQGPAIAARLTDTTRAQLVQAMPGLKERAKTLLELLDNAGFIFADRPLAIDAKGQAVLTPDIRALIG
RLRTALEDVSPWTAATTEAAMRTFAEQAGLKLGAVAQPLRVALTGRTTSPGIFDVLAVLGREECLSRLADQAA

Sequences:

>Translated_473_residues
MTRPVVTRFAPSPTGFLHIGGGRTALFNWLYARKLGGKMLLRIEDTDRQRSTQPAIDAILDGLKWLGIEWDGDTVYQFAR
AARHREVAEQLLAAGKAYRCYATAEELTAMRDKARAEGRSKLYDGSWRDRDPSEAPADMKPTIRLKAPLDGETVIEDQVQ
GRVVWQNENLDDLVLLRGDGTPTYMLAVVVDDHDMDVTHVIRGDDHLINAARQKQIYDAMGWELPVMAHIPLIHGPDGSK
LSKRHGALGVDAYRAMGYLPAALRNYLVRLGWSHGDQEIFTTQEMIDAFDLAAIGRSAARFDFAKLESLNGHYIRQSDDQ
SLVTLLEDLLHYVPQGPAIAARLTDTTRAQLVQAMPGLKERAKTLLELLDNAGFIFADRPLAIDAKGQAVLTPDIRALIG
RLRTALEDVSPWTAATTEAAMRTFAEQAGLKLGAVAQPLRVALTGRTTSPGIFDVLAVLGREECLSRLADQAA
>Mature_472_residues
TRPVVTRFAPSPTGFLHIGGGRTALFNWLYARKLGGKMLLRIEDTDRQRSTQPAIDAILDGLKWLGIEWDGDTVYQFARA
ARHREVAEQLLAAGKAYRCYATAEELTAMRDKARAEGRSKLYDGSWRDRDPSEAPADMKPTIRLKAPLDGETVIEDQVQG
RVVWQNENLDDLVLLRGDGTPTYMLAVVVDDHDMDVTHVIRGDDHLINAARQKQIYDAMGWELPVMAHIPLIHGPDGSKL
SKRHGALGVDAYRAMGYLPAALRNYLVRLGWSHGDQEIFTTQEMIDAFDLAAIGRSAARFDFAKLESLNGHYIRQSDDQS
LVTLLEDLLHYVPQGPAIAARLTDTTRAQLVQAMPGLKERAKTLLELLDNAGFIFADRPLAIDAKGQAVLTPDIRALIGR
LRTALEDVSPWTAATTEAAMRTFAEQAGLKLGAVAQPLRVALTGRTTSPGIFDVLAVLGREECLSRLADQAA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=494, Percent_Identity=30.7692307692308, Blast_Score=207, Evalue=1e-53,
Organism=Escherichia coli, GI1788743, Length=466, Percent_Identity=44.206008583691, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI226510925, Length=244, Percent_Identity=37.2950819672131, Blast_Score=141, Evalue=8e-35,
Organism=Escherichia coli, GI1786895, Length=130, Percent_Identity=30, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71993394, Length=490, Percent_Identity=31.2244897959184, Blast_Score=199, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6324540, Length=489, Percent_Identity=30.4703476482618, Blast_Score=228, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6321192, Length=212, Percent_Identity=30.188679245283, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6324742, Length=112, Percent_Identity=34.8214285714286, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24665321, Length=486, Percent_Identity=32.3045267489712, Blast_Score=238, Evalue=8e-63,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_RHOP2 (Q2IW96)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_486425.1
- ProteinModelPortal:   Q2IW96
- SMR:   Q2IW96
- STRING:   Q2IW96
- GeneID:   3910605
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_2812
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- ProtClustDB:   PRK01406
- BioCyc:   RPAL316058:RPB_2812-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 52168; Mature: 52037

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 243-243

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPVVTRFAPSPTGFLHIGGGRTALFNWLYARKLGGKMLLRIEDTDRQRSTQPAIDAIL
CCCCCEEECCCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHH
DGLKWLGIEWDGDTVYQFARAARHREVAEQLLAAGKAYRCYATAEELTAMRDKARAEGRS
CCHHHEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHCCCH
KLYDGSWRDRDPSEAPADMKPTIRLKAPLDGETVIEDQVQGRVVWQNENLDDLVLLRGDG
HCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHCCCCEEEEECCCCCEEEEEECCC
TPTYMLAVVVDDHDMDVTHVIRGDDHLINAARQKQIYDAMGWELPVMAHIPLIHGPDGSK
CCEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCEEECCCCCH
LSKRHGALGVDAYRAMGYLPAALRNYLVRLGWSHGDQEIFTTQEMIDAFDLAAIGRSAAR
HHHHCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHH
FDFAKLESLNGHYIRQSDDQSLVTLLEDLLHYVPQGPAIAARLTDTTRAQLVQAMPGLKE
HHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCHHH
RAKTLLELLDNAGFIFADRPLAIDAKGQAVLTPDIRALIGRLRTALEDVSPWTAATTEAA
HHHHHHHHHHCCCEEEECCCEEECCCCCEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHH
MRTFAEQAGLKLGAVAQPLRVALTGRTTSPGIFDVLAVLGREECLSRLADQAA
HHHHHHHHCCEECCCCCCEEEEEECCCCCCHHHHHHHHHCHHHHHHHHHHHCC
>Mature Secondary Structure 
TRPVVTRFAPSPTGFLHIGGGRTALFNWLYARKLGGKMLLRIEDTDRQRSTQPAIDAIL
CCCCEEECCCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHH
DGLKWLGIEWDGDTVYQFARAARHREVAEQLLAAGKAYRCYATAEELTAMRDKARAEGRS
CCHHHEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHCCCH
KLYDGSWRDRDPSEAPADMKPTIRLKAPLDGETVIEDQVQGRVVWQNENLDDLVLLRGDG
HCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHCCCCEEEEECCCCCEEEEEECCC
TPTYMLAVVVDDHDMDVTHVIRGDDHLINAARQKQIYDAMGWELPVMAHIPLIHGPDGSK
CCEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCEEECCCCCH
LSKRHGALGVDAYRAMGYLPAALRNYLVRLGWSHGDQEIFTTQEMIDAFDLAAIGRSAAR
HHHHCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHH
FDFAKLESLNGHYIRQSDDQSLVTLLEDLLHYVPQGPAIAARLTDTTRAQLVQAMPGLKE
HHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCHHH
RAKTLLELLDNAGFIFADRPLAIDAKGQAVLTPDIRALIGRLRTALEDVSPWTAATTEAA
HHHHHHHHHHCCCEEEECCCEEECCCCCEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHH
MRTFAEQAGLKLGAVAQPLRVALTGRTTSPGIFDVLAVLGREECLSRLADQAA
HHHHHHHHCCEECCCCCCEEEEEECCCCCCHHHHHHHHHCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA