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The map label for this gene is 86749822

Identifier: 86749822

GI number: 86749822

Start: 3090266

End: 3090925

Strand: Direct

Name: 86749822

Synonym: RPB_2705

Alternate gene names: NA

Gene position: 3090266-3090925 (Clockwise)

Preceding gene: 86749821

Following gene: 86749823

Centisome position: 57.96

GC content: 68.79

Gene sequence:

>660_bases
ATGGATGGCGCGCACGACATGGGCGGCATGGATGGTTTCGGCCCGGTGGTGCCGGAGGCGAACGAGCCGCTGTTTCACGC
CGCATGGGAGCGCCGCGCCTTCGCGCTGACGCTGGCGATGGCGCGGCCCGGCGGCTGGAATCTCGACATGACGCGGTTCG
CCCGCGAGAATCGATCGCCCTTGGACTATCTGTCGAAGAGCTACTACCAGATCTGGCTTGCCGGCCTCGAACGGCTGATG
GCGGAACGTGGACTGGTCACGTCCGACGAGATCGCCGCCGCCCGGCCGCTGCATCCGCGCCGCGACGTCGCAGCACTGTC
GGCGGACGATGCCGCGCCGATGCTGGCGCGCGGCGCGCCGACCGAACGTCCGGCGCCGGCGCCGGCTCATTTCGCGATCG
GCGACCGCGTCCGCGCCAGGAACATCCACCCGCACACCCATACGCGGCTGCCGCGCTATGTCCGCGGCCATGTCGGCGTG
GTCGAGCTGGTGCATGGCGCGCATATTTTTCCGGACAGCCACGCGCTGGGCGCCGGCGAACAGCCACAATGGCTCTACAC
CGTCACTTTCGACGGCCGCGAATTGTGGGGCGACGATTGCGATCCGTCGCTGCGGATCTCGGTCGACGCCTGGGAGAGCT
ATCTGGAGCCTGTGCGATGA

Upstream 100 bases:

>100_bases
ACGGCACCGAAGGCTTCACCGAAGACCAACTGGCCGATCTGGTCACCCGCGACAGCATGATCGGCACCGGCGTGGCACAG
CCACCGGCGGAGAATTCGTG

Downstream 100 bases:

>100_bases
CGGCCGATGCCGCCGCGGTCGCGGCGCGGAGCGTGCCGGGCCTGCCGCGCGACGATGACGGCCCGGTGTTCCGCGAGCCC
TGGGAAGCGCACGCCTTCGC

Product: nitrile hydratase

Products: NA

Alternate protein names: L-NHase; L-nitrilase [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MDGAHDMGGMDGFGPVVPEANEPLFHAAWERRAFALTLAMARPGGWNLDMTRFARENRSPLDYLSKSYYQIWLAGLERLM
AERGLVTSDEIAAARPLHPRRDVAALSADDAAPMLARGAPTERPAPAPAHFAIGDRVRARNIHPHTHTRLPRYVRGHVGV
VELVHGAHIFPDSHALGAGEQPQWLYTVTFDGRELWGDDCDPSLRISVDAWESYLEPVR

Sequences:

>Translated_219_residues
MDGAHDMGGMDGFGPVVPEANEPLFHAAWERRAFALTLAMARPGGWNLDMTRFARENRSPLDYLSKSYYQIWLAGLERLM
AERGLVTSDEIAAARPLHPRRDVAALSADDAAPMLARGAPTERPAPAPAHFAIGDRVRARNIHPHTHTRLPRYVRGHVGV
VELVHGAHIFPDSHALGAGEQPQWLYTVTFDGRELWGDDCDPSLRISVDAWESYLEPVR
>Mature_219_residues
MDGAHDMGGMDGFGPVVPEANEPLFHAAWERRAFALTLAMARPGGWNLDMTRFARENRSPLDYLSKSYYQIWLAGLERLM
AERGLVTSDEIAAARPLHPRRDVAALSADDAAPMLARGAPTERPAPAPAHFAIGDRVRARNIHPHTHTRLPRYVRGHVGV
VELVHGAHIFPDSHALGAGEQPQWLYTVTFDGRELWGDDCDPSLRISVDAWESYLEPVR

Specific function: NHase catalyzes the hydration of various nitrile compounds to the corresponding amides [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrile hydratase subunit beta family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008990
- InterPro:   IPR003168 [H]

Pfam domain/function: NA

EC number: =4.2.1.84 [H]

Molecular weight: Translated: 24331; Mature: 24331

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGAHDMGGMDGFGPVVPEANEPLFHAAWERRAFALTLAMARPGGWNLDMTRFARENRSP
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCEEEEEEEEECCCCCCCHHHHHHHCCCCH
LDYLSKSYYQIWLAGLERLMAERGLVTSDEIAAARPLHPRRDVAALSADDAAPMLARGAP
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCHHEECCCCCCCHHHCCCC
TERPAPAPAHFAIGDRVRARNIHPHTHTRLPRYVRGHVGVVELVHGAHIFPDSHALGAGE
CCCCCCCCCEEECCCCCCCCCCCCCHHHHCCHHHHCCCCHHHHHHCCEECCCCCCCCCCC
QPQWLYTVTFDGRELWGDDCDPSLRISVDAWESYLEPVR
CCCEEEEEEECCHHHCCCCCCCCEEEEHHHHHHHHCCCC
>Mature Secondary Structure
MDGAHDMGGMDGFGPVVPEANEPLFHAAWERRAFALTLAMARPGGWNLDMTRFARENRSP
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCEEEEEEEEECCCCCCCHHHHHHHCCCCH
LDYLSKSYYQIWLAGLERLMAERGLVTSDEIAAARPLHPRRDVAALSADDAAPMLARGAP
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCHHEECCCCCCCHHHCCCC
TERPAPAPAHFAIGDRVRARNIHPHTHTRLPRYVRGHVGVVELVHGAHIFPDSHALGAGE
CCCCCCCCCEEECCCCCCCCCCCCCHHHHCCHHHHCCCCHHHHHHCCEECCCCCCCCCCC
QPQWLYTVTFDGRELWGDDCDPSLRISVDAWESYLEPVR
CCCEEEEEEECCHHHCCCCCCCCEEEEHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1840499 [H]