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The map label for this gene is nthA [H]

Identifier: 86749821

GI number: 86749821

Start: 3089616

End: 3090266

Strand: Direct

Name: nthA [H]

Synonym: RPB_2704

Alternate gene names: 86749821

Gene position: 3089616-3090266 (Clockwise)

Preceding gene: 86749818

Following gene: 86749822

Centisome position: 57.95

GC content: 66.67

Gene sequence:

>651_bases
ATGTTGCGTCGCATGAGCGAACATCAGCACGGCCATTCGCACGATCATGATCACGACCACTCCGAGCTATCGGAGACGGA
ACTGCGCGTGCGCGCGCTCGAAACCATCCTGACCGAGAAGGGCTATGTCGACCCGGCCGCGCTCGACATCCTGATCGAGA
CCTATGAAACCAAGGTCGGGCCGCGCAACGGCGCGCGCGTCGTGGCGAAGGCCTGGACCGACCCCGCCTACCGCGCGCGG
CTGCTGCAAGACGCGACCGCGGCGATCGCCGAACTCGGCTACACCGGCCGCCAGGGCGAGCATATCGTCGCCGTCGAGAA
CACGCCCGCGACGCACAATATGGTCGTGTGCACGCTGTGCTCCTGCTATCCGTGGCCGGTGCTCGGCCTGCCGCCGGTCT
GGTACAAATCCGCGCCGTATCGCTCGCGCGCGGTGAAGGAGCCGCGCGCGGTGCTGGCCGATTTCGGCGTGACCCTGCCC
GACGATACGGCGATCCGGGTGTGGGATTCGACCGCGGAGATCCGCTATCTGGTGATTCCGATGCGGCCCGACGGCACCGA
AGGCTTCACCGAAGACCAACTGGCCGATCTGGTCACCCGCGACAGCATGATCGGCACCGGCGTGGCACAGCCACCGGCGG
AGAATTCGTGA

Upstream 100 bases:

>100_bases
TCGATTGTCACGAACTGCCCCACGATGCCTCCCGAACTGCATTCTTGGTTGTCCGAAGGCTAGCAGCTTCCCGGCTCGAC
AGCGCAACATTTTCTAATCT

Downstream 100 bases:

>100_bases
TGGATGGCGCGCACGACATGGGCGGCATGGATGGTTTCGGCCCGGTGGTGCCGGAGGCGAACGAGCCGCTGTTTCACGCC
GCATGGGAGCGCCGCGCCTT

Product: nitrile hydratase, alpha subunit

Products: NA

Alternate protein names: NHase; Nitrilase [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MLRRMSEHQHGHSHDHDHDHSELSETELRVRALETILTEKGYVDPAALDILIETYETKVGPRNGARVVAKAWTDPAYRAR
LLQDATAAIAELGYTGRQGEHIVAVENTPATHNMVVCTLCSCYPWPVLGLPPVWYKSAPYRSRAVKEPRAVLADFGVTLP
DDTAIRVWDSTAEIRYLVIPMRPDGTEGFTEDQLADLVTRDSMIGTGVAQPPAENS

Sequences:

>Translated_216_residues
MLRRMSEHQHGHSHDHDHDHSELSETELRVRALETILTEKGYVDPAALDILIETYETKVGPRNGARVVAKAWTDPAYRAR
LLQDATAAIAELGYTGRQGEHIVAVENTPATHNMVVCTLCSCYPWPVLGLPPVWYKSAPYRSRAVKEPRAVLADFGVTLP
DDTAIRVWDSTAEIRYLVIPMRPDGTEGFTEDQLADLVTRDSMIGTGVAQPPAENS
>Mature_216_residues
MLRRMSEHQHGHSHDHDHDHSELSETELRVRALETILTEKGYVDPAALDILIETYETKVGPRNGARVVAKAWTDPAYRAR
LLQDATAAIAELGYTGRQGEHIVAVENTPATHNMVVCTLCSCYPWPVLGLPPVWYKSAPYRSRAVKEPRAVLADFGVTLP
DDTAIRVWDSTAEIRYLVIPMRPDGTEGFTEDQLADLVTRDSMIGTGVAQPPAENS

Specific function: NHase catalyzes the hydration of various nitrile compounds to the corresponding amides. Industrial production of acrylamide is now being developed using some of the enzymes of this class [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrile hydratase subunit alpha family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004232
- InterPro:   IPR018141
- ProDom:   PD007559 [H]

Pfam domain/function: PF02979 NHase_alpha [H]

EC number: =4.2.1.84 [H]

Molecular weight: Translated: 23955; Mature: 23955

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRRMSEHQHGHSHDHDHDHSELSETELRVRALETILTEKGYVDPAALDILIETYETKVG
CCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
PRNGARVVAKAWTDPAYRARLLQDATAAIAELGYTGRQGEHIVAVENTPATHNMVVCTLC
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEEC
SCYPWPVLGLPPVWYKSAPYRSRAVKEPRAVLADFGVTLPDDTAIRVWDSTAEIRYLVIP
CCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHCCCCCCCCCEEEEECCCCCEEEEEEE
MRPDGTEGFTEDQLADLVTRDSMIGTGVAQPPAENS
ECCCCCCCCCHHHHHHHHHHCCHHCCCCCCCCCCCC
>Mature Secondary Structure
MLRRMSEHQHGHSHDHDHDHSELSETELRVRALETILTEKGYVDPAALDILIETYETKVG
CCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
PRNGARVVAKAWTDPAYRARLLQDATAAIAELGYTGRQGEHIVAVENTPATHNMVVCTLC
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEEC
SCYPWPVLGLPPVWYKSAPYRSRAVKEPRAVLADFGVTLPDDTAIRVWDSTAEIRYLVIP
CCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHCCCCCCCCCEEEEECCCCCEEEEEEE
MRPDGTEGFTEDQLADLVTRDSMIGTGVAQPPAENS
ECCCCCCCCCHHHHHHHHHHCCHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9154927 [H]