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The map label for this gene is mscL

Identifier: 86749721

GI number: 86749721

Start: 2988060

End: 2988491

Strand: Direct

Name: mscL

Synonym: RPB_2603

Alternate gene names: 86749721

Gene position: 2988060-2988491 (Clockwise)

Preceding gene: 86749720

Following gene: 86749722

Centisome position: 56.04

GC content: 64.81

Gene sequence:

>432_bases
ATGTTCAAGGAATTCCGTGAATTCGCGATGAAGGGCAACGTCGTCGACCTCGCGGTCGGCGTCATCATCGGTGCGGCGTT
CGGCGGCATCGTCAGTTCGCTGGTGGCCGACGTCATCATGCCGATCATCGGCGCGATCACCGGCGGGCTCGATTTCTCGA
ACTACTTCACGCCGCTGTCGAAGGCGGTGACGGCGAACACGCTGGTGGAAGCGAAGAAGCAGGGCGCGGTGCTGGCCTGG
GGCAGCTTTCTCACGCTGACGCTGAACTTCCTGATCGTCGCCTTCGTGCTGTTCCTGGTGGTGCGGGCGATGAACCGGCT
GAAGCGCAAGGACGAGGCCGCCCCCGCGCCGCCGGCGAAGCCGACCCCGGAGCAGGAACTGCTCGCCGAGATACGCGATA
TCCTGAAGAGCGGCAGCCGCCCGCAGGCTTGA

Upstream 100 bases:

>100_bases
CGGCGCCACGATCCGCGCCGCCATGATGCATGCCGGACTGCTGAACTGATTCACGCGGAATTGCAGGACAGGCTGCGCAA
CGACCAGGGCCGAGGGCGGT

Downstream 100 bases:

>100_bases
GCCTGCAGCGGAGCGGCGACAACGAGGTTCAGCCGCAATGGCCGAGAAGAACGAACGCGCGATCAAGGTGGTCGCGGAGA
ACCGCAAGGCGCGGTTCAAC

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 143; Mature: 143

Protein sequence:

>143_residues
MFKEFREFAMKGNVVDLAVGVIIGAAFGGIVSSLVADVIMPIIGAITGGLDFSNYFTPLSKAVTANTLVEAKKQGAVLAW
GSFLTLTLNFLIVAFVLFLVVRAMNRLKRKDEAAPAPPAKPTPEQELLAEIRDILKSGSRPQA

Sequences:

>Translated_143_residues
MFKEFREFAMKGNVVDLAVGVIIGAAFGGIVSSLVADVIMPIIGAITGGLDFSNYFTPLSKAVTANTLVEAKKQGAVLAW
GSFLTLTLNFLIVAFVLFLVVRAMNRLKRKDEAAPAPPAKPTPEQELLAEIRDILKSGSRPQA
>Mature_143_residues
MFKEFREFAMKGNVVDLAVGVIIGAAFGGIVSSLVADVIMPIIGAITGGLDFSNYFTPLSKAVTANTLVEAKKQGAVLAW
GSFLTLTLNFLIVAFVLFLVVRAMNRLKRKDEAAPAPPAKPTPEQELLAEIRDILKSGSRPQA

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=137, Percent_Identity=52.5547445255474, Blast_Score=134, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_RHOP2 (Q2IWV4)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_486217.1
- ProteinModelPortal:   Q2IWV4
- SMR:   Q2IWV4
- STRING:   Q2IWV4
- GeneID:   3910395
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_2603
- eggNOG:   COG1970
- HOGENOM:   HBG727604
- OMA:   GANVLAW
- ProtClustDB:   PRK13952
- BioCyc:   RPAL316058:RPB_2603-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 15291; Mature: 15291

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1c10e988)-; HASH(0x1e6e5300)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKEFREFAMKGNVVDLAVGVIIGAAFGGIVSSLVADVIMPIIGAITGGLDFSNYFTPLS
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
KAVTANTLVEAKKQGAVLAWGSFLTLTLNFLIVAFVLFLVVRAMNRLKRKDEAAPAPPAK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PTPEQELLAEIRDILKSGSRPQA
CCCHHHHHHHHHHHHHCCCCCCH
>Mature Secondary Structure
MFKEFREFAMKGNVVDLAVGVIIGAAFGGIVSSLVADVIMPIIGAITGGLDFSNYFTPLS
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
KAVTANTLVEAKKQGAVLAWGSFLTLTLNFLIVAFVLFLVVRAMNRLKRKDEAAPAPPAK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PTPEQELLAEIRDILKSGSRPQA
CCCHHHHHHHHHHHHHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA