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The map label for this gene is nqo12 [H]

Identifier: 86749703

GI number: 86749703

Start: 2965272

End: 2967377

Strand: Direct

Name: nqo12 [H]

Synonym: RPB_2585

Alternate gene names: 86749703

Gene position: 2965272-2967377 (Clockwise)

Preceding gene: 86749702

Following gene: 86749704

Centisome position: 55.62

GC content: 64.48

Gene sequence:

>2106_bases
ATGATCCAGGCTATCGTATTCCTGCCGCTGATCGGCGCGCTGATCGCCGCAATCATCGCGCTCGCTGGCGCCCATGCCCG
GCACCCGAGCGGCGACACCATGGACCACGGCGATCATGGTCACGGCCACGCCGCGCATGGTCATGCTGCGCACGGCCATG
CCGCCGATGCGCACGCCCACGATGCGCACGCCCACGATGATCACGGCCACGACGATCACGGTCACGGTCCGGTCGAGCCC
GCGGCGTTCGGTTCGCGCACGACCGAGGTGATCACCACCGCGCTGCTGATGATCTCCTGCGCGCTGTCCTGGGTGACGCT
GGTCGACGTCGGCTTCATGCATCACGATGCCCGCGTCGCGCTGTTCCCGTGGATCAATTCCGGCGACCTGCAGGTCGCCT
GGGCGCTGCGGGTCGACACCCTGACCGCGGTGATGCTCGTCGTGGTGACGACGGTGTCGTGCCTCGTCCATCTGTATTCG
ATCGGCTACATGCACGAGGACCCGCACCGGCCGCGGTTCTTCGGCTATCTGTCGCTGTTCACCTTCGCGATGCTGATGCT
GGTGACCGCCGACAATCTGGTGCAGCTGTTCTTCGGCTGGGAAGGCGTCGGTCTGGCGAGCTACCTGCTGATCGGCTTCT
GGTACCAGAAGCCGTCCGCCAATGCCGCCGCGATCAAGGCCTTCGTCGTCAACCGCGTCGGCGATTTCGGCTTCGCGCTG
GGTATCTTCGCGATATTCATGCTGGTCGGCTCGACCGATTTCGACACCATCTTCGCGGCAGCGCCCGGGCTGACCGGCAA
GACCATCAACTTCTTCGGGTGGAACGCCGACGCGCTGACGCTGACCTGCCTGTTGCTGTTCATGGGCGCGATGGGCAAGT
CAGCGCAGTTCCTGCTGCACACCTGGCTGCCGGACGCGATGGAAGGCCCGACGCCGGTGTCGGCGCTGATCCACGCCGCC
ACCATGGTCACCGCCGGCGTGTTCATGGTGGCGCGGCTGTCGCCGCTGTTCGAACTGGCTCCGAACGCGCAGGCCTTCGT
GATGCTGATCGGCGGCACCACGGCGTTGTTCGCCGCGACGATCGGTCTGGTGCAGAACGACATCAAGCGGATCGTGGCGT
ATTCGACTTGTTCGCAGCTCGGCTACATGTTCGTGGCGATGGGCGCGGGCGCCTATTCGGTCGGCATGTTCCACCTGTTC
ACCCACGCCTTCTTCAAGGCGCTGCTTTTCCTGGGCGCCGGCTCGGTGATCCACGCGATGCACCACGAGCAGGACATCCG
CCACATGGGCGGGCTGAAGGACAAGATCCCCTTCACCTACATCGTGATGGTGATCGGCACGCTGGCGCTGACCGGCTTCC
CGCTGACCGCCGGCTACTTCTCCAAGGACGCGATCATCGAGTCCGCCTACGTCTCGCACAATCCGATGTCGTTCTACGGC
TTCCTGATGACCGTCGTCGCCGCGCTGCTGACCTCGTTCTATTCGTGGCGGCTGATCTTCAAGACCTTCCACGGCGAGCC
GCACGACCGGAAGCACTACGAGGCCGCGCACGAAAGCCCGCTGACGATGACGATCCCGCTGTTCGTGCTCGCGGTCGGCG
CGCTGGCGGCCGGCTATCCGTTCAAGGAGCTGTTCGCCGGCCACGGCGTCGAGGAGTTCTTCCGCAATTCGCTGAAGATG
CATCCCGGCATCATCGAGGAGATGCATCACATCCCGGCCGGGATCGCGTATCTGCCGACGGTGATGATGGCGGTGGGCTT
CCTGGTGTCGTGGATGTTCTACATCCGCCGGCCGTATCTGCCGGTCGAGCTCGCGACCCAGCATCCGCTGCTGTACAATT
TCCTGCTCAACAAATGGTACTTCGACGAATTGTACGAGGTGATCTTCGTGCGGCCGGCGAAGTGGATCGGCCGGCAACTC
TGGAAGAAGGGTGACGGCATGCTGATCGACGGCCTCGGCCCCGACGGCGTCTCGGCCCGGGTGCTCGACGTCACCCGCGG
TGTCGTCAAGCTGCAGTCCGGCTATCTCTACCACTACGCATTCGCGATGCTGATCGGCGTGGCCGGTCTGATCACCTGGT
TCATGCTCGGCGGGGGAGGCCGCTAA

Upstream 100 bases:

>100_bases
TCGCGGTTGAAGATATCAACCTGATGAAGGGCTAGGGCATGATCGTCCATCAAGCGTCGCGGACCCGCGGCGCGATCCGG
CTCCCGGCGAAGGCGCGCGC

Downstream 100 bases:

>100_bases
TGTCGGCTTCAATCATGACCTGGCCGGTGCTGTCGGTCGTCACCTTCCTGCCGCTGGTCGGCGCGGCGCTGGTCTATCTC
GCTCGCGGCGATGACGAGGC

Product: NADH dehydrogenase subunit L

Products: NA

Alternate protein names: NADH dehydrogenase I, chain 12; NDH-1, chain 12 [H]

Number of amino acids: Translated: 701; Mature: 701

Protein sequence:

>701_residues
MIQAIVFLPLIGALIAAIIALAGAHARHPSGDTMDHGDHGHGHAAHGHAAHGHAADAHAHDAHAHDDHGHDDHGHGPVEP
AAFGSRTTEVITTALLMISCALSWVTLVDVGFMHHDARVALFPWINSGDLQVAWALRVDTLTAVMLVVVTTVSCLVHLYS
IGYMHEDPHRPRFFGYLSLFTFAMLMLVTADNLVQLFFGWEGVGLASYLLIGFWYQKPSANAAAIKAFVVNRVGDFGFAL
GIFAIFMLVGSTDFDTIFAAAPGLTGKTINFFGWNADALTLTCLLLFMGAMGKSAQFLLHTWLPDAMEGPTPVSALIHAA
TMVTAGVFMVARLSPLFELAPNAQAFVMLIGGTTALFAATIGLVQNDIKRIVAYSTCSQLGYMFVAMGAGAYSVGMFHLF
THAFFKALLFLGAGSVIHAMHHEQDIRHMGGLKDKIPFTYIVMVIGTLALTGFPLTAGYFSKDAIIESAYVSHNPMSFYG
FLMTVVAALLTSFYSWRLIFKTFHGEPHDRKHYEAAHESPLTMTIPLFVLAVGALAAGYPFKELFAGHGVEEFFRNSLKM
HPGIIEEMHHIPAGIAYLPTVMMAVGFLVSWMFYIRRPYLPVELATQHPLLYNFLLNKWYFDELYEVIFVRPAKWIGRQL
WKKGDGMLIDGLGPDGVSARVLDVTRGVVKLQSGYLYHYAFAMLIGVAGLITWFMLGGGGR

Sequences:

>Translated_701_residues
MIQAIVFLPLIGALIAAIIALAGAHARHPSGDTMDHGDHGHGHAAHGHAAHGHAADAHAHDAHAHDDHGHDDHGHGPVEP
AAFGSRTTEVITTALLMISCALSWVTLVDVGFMHHDARVALFPWINSGDLQVAWALRVDTLTAVMLVVVTTVSCLVHLYS
IGYMHEDPHRPRFFGYLSLFTFAMLMLVTADNLVQLFFGWEGVGLASYLLIGFWYQKPSANAAAIKAFVVNRVGDFGFAL
GIFAIFMLVGSTDFDTIFAAAPGLTGKTINFFGWNADALTLTCLLLFMGAMGKSAQFLLHTWLPDAMEGPTPVSALIHAA
TMVTAGVFMVARLSPLFELAPNAQAFVMLIGGTTALFAATIGLVQNDIKRIVAYSTCSQLGYMFVAMGAGAYSVGMFHLF
THAFFKALLFLGAGSVIHAMHHEQDIRHMGGLKDKIPFTYIVMVIGTLALTGFPLTAGYFSKDAIIESAYVSHNPMSFYG
FLMTVVAALLTSFYSWRLIFKTFHGEPHDRKHYEAAHESPLTMTIPLFVLAVGALAAGYPFKELFAGHGVEEFFRNSLKM
HPGIIEEMHHIPAGIAYLPTVMMAVGFLVSWMFYIRRPYLPVELATQHPLLYNFLLNKWYFDELYEVIFVRPAKWIGRQL
WKKGDGMLIDGLGPDGVSARVLDVTRGVVKLQSGYLYHYAFAMLIGVAGLITWFMLGGGGR
>Mature_701_residues
MIQAIVFLPLIGALIAAIIALAGAHARHPSGDTMDHGDHGHGHAAHGHAAHGHAADAHAHDAHAHDDHGHDDHGHGPVEP
AAFGSRTTEVITTALLMISCALSWVTLVDVGFMHHDARVALFPWINSGDLQVAWALRVDTLTAVMLVVVTTVSCLVHLYS
IGYMHEDPHRPRFFGYLSLFTFAMLMLVTADNLVQLFFGWEGVGLASYLLIGFWYQKPSANAAAIKAFVVNRVGDFGFAL
GIFAIFMLVGSTDFDTIFAAAPGLTGKTINFFGWNADALTLTCLLLFMGAMGKSAQFLLHTWLPDAMEGPTPVSALIHAA
TMVTAGVFMVARLSPLFELAPNAQAFVMLIGGTTALFAATIGLVQNDIKRIVAYSTCSQLGYMFVAMGAGAYSVGMFHLF
THAFFKALLFLGAGSVIHAMHHEQDIRHMGGLKDKIPFTYIVMVIGTLALTGFPLTAGYFSKDAIIESAYVSHNPMSFYG
FLMTVVAALLTSFYSWRLIFKTFHGEPHDRKHYEAAHESPLTMTIPLFVLAVGALAAGYPFKELFAGHGVEEFFRNSLKM
HPGIIEEMHHIPAGIAYLPTVMMAVGFLVSWMFYIRRPYLPVELATQHPLLYNFLLNKWYFDELYEVIFVRPAKWIGRQL
WKKGDGMLIDGLGPDGVSARVLDVTRGVVKLQSGYLYHYAFAMLIGVAGLITWFMLGGGGR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=434, Percent_Identity=42.8571428571429, Blast_Score=320, Evalue=2e-87,
Organism=Escherichia coli, GI1788614, Length=570, Percent_Identity=40.8771929824561, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI1788829, Length=451, Percent_Identity=29.0465631929047, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1788827, Length=430, Percent_Identity=28.3720930232558, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1788831, Length=348, Percent_Identity=30.4597701149425, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI2367154, Length=264, Percent_Identity=27.6515151515151, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1788613, Length=418, Percent_Identity=23.6842105263158, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI145693160, Length=366, Percent_Identity=23.7704918032787, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 76622; Mature: 76622

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQAIVFLPLIGALIAAIIALAGAHARHPSGDTMDHGDHGHGHAAHGHAAHGHAADAHAH
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DAHAHDDHGHDDHGHGPVEPAAFGSRTTEVITTALLMISCALSWVTLVDVGFMHHDARVA
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LFPWINSGDLQVAWALRVDTLTAVMLVVVTTVSCLVHLYSIGYMHEDPHRPRFFGYLSLF
EEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
TFAMLMLVTADNLVQLFFGWEGVGLASYLLIGFWYQKPSANAAAIKAFVVNRVGDFGFAL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
GIFAIFMLVGSTDFDTIFAAAPGLTGKTINFFGWNADALTLTCLLLFMGAMGKSAQFLLH
HHHHHHHHHCCCCHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHH
TWLPDAMEGPTPVSALIHAATMVTAGVFMVARLSPLFELAPNAQAFVMLIGGTTALFAAT
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHH
IGLVQNDIKRIVAYSTCSQLGYMFVAMGAGAYSVGMFHLFTHAFFKALLFLGAGSVIHAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
HHEQDIRHMGGLKDKIPFTYIVMVIGTLALTGFPLTAGYFSKDAIIESAYVSHNPMSFYG
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHH
FLMTVVAALLTSFYSWRLIFKTFHGEPHDRKHYEAAHESPLTMTIPLFVLAVGALAAGYP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEHHHHHHHHHHHHHCCCC
FKELFAGHGVEEFFRNSLKMHPGIIEEMHHIPAGIAYLPTVMMAVGFLVSWMFYIRRPYL
HHHHHHCCCHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
PVELATQHPLLYNFLLNKWYFDELYEVIFVRPAKWIGRQLWKKGDGMLIDGLGPDGVSAR
CEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEECCCCCCCCHH
VLDVTRGVVKLQSGYLYHYAFAMLIGVAGLITWFMLGGGGR
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIQAIVFLPLIGALIAAIIALAGAHARHPSGDTMDHGDHGHGHAAHGHAAHGHAADAHAH
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DAHAHDDHGHDDHGHGPVEPAAFGSRTTEVITTALLMISCALSWVTLVDVGFMHHDARVA
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LFPWINSGDLQVAWALRVDTLTAVMLVVVTTVSCLVHLYSIGYMHEDPHRPRFFGYLSLF
EEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
TFAMLMLVTADNLVQLFFGWEGVGLASYLLIGFWYQKPSANAAAIKAFVVNRVGDFGFAL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
GIFAIFMLVGSTDFDTIFAAAPGLTGKTINFFGWNADALTLTCLLLFMGAMGKSAQFLLH
HHHHHHHHHCCCCHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHH
TWLPDAMEGPTPVSALIHAATMVTAGVFMVARLSPLFELAPNAQAFVMLIGGTTALFAAT
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHH
IGLVQNDIKRIVAYSTCSQLGYMFVAMGAGAYSVGMFHLFTHAFFKALLFLGAGSVIHAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
HHEQDIRHMGGLKDKIPFTYIVMVIGTLALTGFPLTAGYFSKDAIIESAYVSHNPMSFYG
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHH
FLMTVVAALLTSFYSWRLIFKTFHGEPHDRKHYEAAHESPLTMTIPLFVLAVGALAAGYP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEHHHHHHHHHHHHHCCCC
FKELFAGHGVEEFFRNSLKMHPGIIEEMHHIPAGIAYLPTVMMAVGFLVSWMFYIRRPYL
HHHHHHCCCHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
PVELATQHPLLYNFLLNKWYFDELYEVIFVRPAKWIGRQLWKKGDGMLIDGLGPDGVSAR
CEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEECCCCCCCCHH
VLDVTRGVVKLQSGYLYHYAFAMLIGVAGLITWFMLGGGGR
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]