Click here to switch to the map view.

The map label for this gene is purN [H]

Identifier: 86749608

GI number: 86749608

Start: 2846400

End: 2847056

Strand: Reverse

Name: purN [H]

Synonym: RPB_2488

Alternate gene names: 86749608

Gene position: 2847056-2846400 (Counterclockwise)

Preceding gene: 86749609

Following gene: 86749606

Centisome position: 53.4

GC content: 65.14

Gene sequence:

>657_bases
GTGAGCAAGCGTCGTGTCGCCATCCTGATTTCCGGGCGCGGGTCGAACATGGCCGCGCTGATCGAAGCCGCGGCCGAAGA
CGGCTTCCCTGCCGACATTGCGGTCGTGATCGCCAACACCGCGAGCGCCGGCGGCCTTGCGATCGCTCAACGCAGCGGGA
TTGAAACGCTGGTGATCGAAAGCAAGCCGTTCGGCAAGGATCGCGCCGGCTTCGAAGCCGTGTTGCAGGCGGCGCTTGAC
GCACGCGGCATCGAGCTGATCTGCCTCGGCGGCTTCATGCGGCTGTTCACGGCGGATTTCGTCAATCATTGGTACGGCCG
GATGCTCAACATTCATCCGTCGCTGCTGCCGTCCTTCCCGGGCCTCGATCCGCACGGTCAGGCCCTGCGCGCCGGCGTGA
AGATTTCCGGCGCCACCGTGCATTTCGTCATCGCGGAAACCGACGCCGGACCGATCGTGATTCAAGGCGCGGTGCCGGTG
CACGACGACGACACCGCCGACACGCTGGCCGACCGCGTCCTGGCGATCGAGCACCGGATCTATCCGAGAGCCCTGCAGAT
GGTGGCATCCGGCCAAACGCGATTCGAGGGCGATCTGTGCAGATCGACCTCGAGTGGCCCGCATGATCAGGCGCTGATCG
TACCGTCGGAGAACTGA

Upstream 100 bases:

>100_bases
GCGGGCGGCGAGAGCGTGCATCTGCTCGGCGAAGTGATCGCGGCGAAGGGCGAACATCGCGTCGTCTACGACGGTCACCT
CGACCTGTCCTGGTGATCGC

Downstream 100 bases:

>100_bases
TCGAGACGATCGAACCTGCTCACGTGAGCTTGAGGTTCTCCGCCGAGGTCTTGCCGCGATTTTCGACCAGATCGTATTCG
ATCGCCTGGTTTTCATTCAG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MSKRRVAILISGRGSNMAALIEAAAEDGFPADIAVVIANTASAGGLAIAQRSGIETLVIESKPFGKDRAGFEAVLQAALD
ARGIELICLGGFMRLFTADFVNHWYGRMLNIHPSLLPSFPGLDPHGQALRAGVKISGATVHFVIAETDAGPIVIQGAVPV
HDDDTADTLADRVLAIEHRIYPRALQMVASGQTRFEGDLCRSTSSGPHDQALIVPSEN

Sequences:

>Translated_218_residues
MSKRRVAILISGRGSNMAALIEAAAEDGFPADIAVVIANTASAGGLAIAQRSGIETLVIESKPFGKDRAGFEAVLQAALD
ARGIELICLGGFMRLFTADFVNHWYGRMLNIHPSLLPSFPGLDPHGQALRAGVKISGATVHFVIAETDAGPIVIQGAVPV
HDDDTADTLADRVLAIEHRIYPRALQMVASGQTRFEGDLCRSTSSGPHDQALIVPSEN
>Mature_217_residues
SKRRVAILISGRGSNMAALIEAAAEDGFPADIAVVIANTASAGGLAIAQRSGIETLVIESKPFGKDRAGFEAVLQAALDA
RGIELICLGGFMRLFTADFVNHWYGRMLNIHPSLLPSFPGLDPHGQALRAGVKISGATVHFVIAETDAGPIVIQGAVPVH
DDDTADTLADRVLAIEHRIYPRALQMVASGQTRFEGDLCRSTSSGPHDQALIVPSEN

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=201, Percent_Identity=49.2537313432836, Blast_Score=195, Evalue=3e-50,
Organism=Homo sapiens, GI209869995, Length=201, Percent_Identity=49.2537313432836, Blast_Score=195, Evalue=3e-50,
Organism=Homo sapiens, GI209869993, Length=201, Percent_Identity=49.2537313432836, Blast_Score=195, Evalue=3e-50,
Organism=Escherichia coli, GI1788846, Length=190, Percent_Identity=39.4736842105263, Blast_Score=146, Evalue=9e-37,
Organism=Escherichia coli, GI1787483, Length=196, Percent_Identity=30.6122448979592, Blast_Score=94, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17567511, Length=186, Percent_Identity=42.4731182795699, Blast_Score=143, Evalue=8e-35,
Organism=Drosophila melanogaster, GI24582400, Length=191, Percent_Identity=48.6910994764398, Blast_Score=175, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23020; Mature: 22888

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRRVAILISGRGSNMAALIEAAAEDGFPADIAVVIANTASAGGLAIAQRSGIETLVIE
CCCCEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEEEECCCCEEEEEE
SKPFGKDRAGFEAVLQAALDARGIELICLGGFMRLFTADFVNHWYGRMLNIHPSLLPSFP
CCCCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHCEEECCHHHCCCCC
GLDPHGQALRAGVKISGATVHFVIAETDAGPIVIQGAVPVHDDDTADTLADRVLAIEHRI
CCCCCCHHHHCCEEECCCEEEEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHH
YPRALQMVASGQTRFEGDLCRSTSSGPHDQALIVPSEN
HHHHHHHHHCCCCCCCCHHHCCCCCCCCCCEEEEECCC
>Mature Secondary Structure 
SKRRVAILISGRGSNMAALIEAAAEDGFPADIAVVIANTASAGGLAIAQRSGIETLVIE
CCCEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEEEECCCCEEEEEE
SKPFGKDRAGFEAVLQAALDARGIELICLGGFMRLFTADFVNHWYGRMLNIHPSLLPSFP
CCCCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHCEEECCHHHCCCCC
GLDPHGQALRAGVKISGATVHFVIAETDAGPIVIQGAVPVHDDDTADTLADRVLAIEHRI
CCCCCCHHHHCCEEECCCEEEEEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHH
YPRALQMVASGQTRFEGDLCRSTSSGPHDQALIVPSEN
HHHHHHHHHCCCCCCCCHHHCCCCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]