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The map label for this gene is lptD [H]

Identifier: 86749598

GI number: 86749598

Start: 2834360

End: 2836861

Strand: Reverse

Name: lptD [H]

Synonym: RPB_2478

Alternate gene names: 86749598

Gene position: 2836861-2834360 (Counterclockwise)

Preceding gene: 86749599

Following gene: 86749597

Centisome position: 53.21

GC content: 62.15

Gene sequence:

>2502_bases
ATGATCGCCGCCCTCCGAGACGAGCTGTTCGCCTTGCCGCGCCGCACCATCGTGCGCAGCAAGCGCTCCCTCATGGCCAG
TTGTGCGATTCCGTTGGCTGCCCTGCTGCTGGCAGGCGCGGCCGACCTCGCGTCGATCTCGTCGGCGGAAGCGCAGAGCT
ACACCTATAATCCGCGCCCGGCCCGGCCGCGTCCGCCCCAGACGGGGACGGACGGGCAGATGCTCGTCCAGGCGACAGAG
GTCAACTACGACTACAACAACCAGCGGGTGTCCGCCGTCGGCAACGTCCAGATGTTCTTCAACGGAACCAGTGTCGAAGC
CGACCGCCTGGTCTACGACCAGAACACCAAGCGTCTGCGCGCCGAAGGCAACGTCCGAATGACGGACGCGACGGGCAAGA
TCACCTACGCCAATATGCTCGATCTGAGCGACGACTACCGCGACGGTTTCGTCGATTCGCTGCGCGTCGACACCGCCGAA
GACACCCGTATCGCAGCGTCGCGCGCCGATCGCACCGACGGCGACTACAACGTGTTTCAGAACGGCGTGTACACCGCCTG
CGCGCCGTGCCGGGACGATCCGAAGAAGCCGCCGCTGTGGCAGGTCAAGGGCGCCCGGATCATCCACGATCAAGTCGACA
AAATGCTGTATTTCGAGAACGCCCAGCTCGAATTCTTCGGCGTTCCGATGGCCTATCTGCCGTATTTCTCGACGCCCGAT
CCGACGGTGAAGCGTAAGACCGGCTTCCTGATGCCGTTCTACACCACCAACACGACCTTCGGGATGGGCTTCGAAATCCC
GTTCTATTGGGCGCTCGCACCGGATTACGACGTCACGCTGACGCCCCGGATCACGACCAAGCAGGGCGTGCTGATGCAAG
GCGAGTTCCGGCAGCGATTGATGGACGGCGCCTATCAGATCCGCGCCTATGGCATCAGCCAGTCCGACCCCGCGGCGTTC
GGAAGCGCGCCGGGCAATCGCTCGTTGCGTGGCGGCGTCGACACCAAAGGCGAATTCGCGCTGAACGACAAATGGGTGTT
CGGCTGGGAAGGCGTGTTGCTGTCCGACCGTGCGTTCTTCCTCGACTACAGGCTCGCGCAGTATCGCGACAGTTGGGGCA
GCTTCCTCAACCAGAGCACCGAAGCGACCTCGCAGATCTATCTGAGCGGCGTCGGCAACCGCTCCTATTTCGATTTGCGG
GCGATTCACTATCTCGGCTGGGCATCGGCTGACATTCAGGGCCAGATTCCGGTCATCCACCCGGTGCTGGACTATTCGAA
GACTCTGGATCGCAACATCTTCGGCGGCGAAGTCAGCTTCAAGACCAACTTCACCAGCCTCTCCCGCCAGACGGCGCAGT
TCGACCCGATCACGACGATCGCGAATACGACGAGCCTTTGTCTGAACACATCGGCCGATCCGGCTGCGCGCATGCCGTCG
AGCTGCCTGTTGCGCGGCATTCCGGGCACCTACACGCGTGCAACGGCCGAGGCGCAATGGCGCCGGTCGTTCACCGACCC
CTACGGCCAGATCTGGACCCCATTCGCATCGATCCGCATGGACGCGATCGATTCCTCGGTCTCGAATCAGCCCGGCGTGT
CGAACTATCTCCCTGTCGGAGACACCCAGGCGTTCCGGGTGATGCCGACCATCGGTCTCGAATATCGCTACCCCTTCATC
AACGTGCAGCCCTGGGGCACCACGACGATCGAACCGATCGCGCAGGTCATCGTTCGTCCGAACGAAACCTACGCGGGCAA
ACTGCCGAACGAGGACGCGCAGAGCATGGTGTTCGACACCAGCAATCTGTTCAGCGTCGACAAATTCTCCGGCTACGATC
GCGTCGAAGGCGGCGGCCGCGCCAATGTCGGCGTCCAGGCGACCACGCAATTCGATCGCGGTGGCGCGATCAACGTGCTG
TTCGGCCAGTCCTATCAGTTGTTTGGGCAGAACTCCTACGCGGTGAGGGACACCACCAATACCGGTCTCGATTCGGGGCT
CGCGACAGCCCGTTCCGACTATGTCGGCCGGGTCTCATATTCGCCGAATTCGACCTACAAGTTCACCACACGTGCGCGAC
TCGACGAGGCGACGCTCGACGTCAACCGCTTCGAGGCCGAAGCCAGCGCGTCGTTCAATCGCTGGTCCGTCAGCGTGATC
TACGGGAACTACGCGGCGCAACCGGACCTCGGATTTCTGACGCGACGTCAGGGCATCCTCACCACCGGTTCCATCAAGGT
GGCGTCGAATTGGGTCGTCTCGGGTGGCGCCCGCTGGGACCTTGAGGCAAACCGCATCAATCAATATATTGTTGGTGCCG
GCTACGTGGACGACTGTTTCGTGATGGCCGTGAACTATGTCACCGGCTATTCGTACGCCAACTACGGAACGACGCCGACG
CTGAATCATTCGGTCATGCTGCAGATCGGACTTCGGACCATCGGCATGGGCGCGATGCAACAGAGCGTTTCGGGCGCGTC
GAGCGGCGTTTTCGGCCAATAG

Upstream 100 bases:

>100_bases
CTGAGTTGATGCATCCGCTTGCTGCAGCGTGGTTGCCCGTCTGTGTGGGCGGCCTCGCTGGTTTTTTGGCTTTGCTGTAC
CAGGAGGACGGGTAGTGGCC

Downstream 100 bases:

>100_bases
CGCCGAAACACATACGGGCAGCCCCCGTTTCTGATCAATCACATCGGCTGGGAGGCATCTGCGACCCATGACGACCAAGT
TCTCCTTTCGTATTTTCGCT

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 833; Mature: 833

Protein sequence:

>833_residues
MIAALRDELFALPRRTIVRSKRSLMASCAIPLAALLLAGAADLASISSAEAQSYTYNPRPARPRPPQTGTDGQMLVQATE
VNYDYNNQRVSAVGNVQMFFNGTSVEADRLVYDQNTKRLRAEGNVRMTDATGKITYANMLDLSDDYRDGFVDSLRVDTAE
DTRIAASRADRTDGDYNVFQNGVYTACAPCRDDPKKPPLWQVKGARIIHDQVDKMLYFENAQLEFFGVPMAYLPYFSTPD
PTVKRKTGFLMPFYTTNTTFGMGFEIPFYWALAPDYDVTLTPRITTKQGVLMQGEFRQRLMDGAYQIRAYGISQSDPAAF
GSAPGNRSLRGGVDTKGEFALNDKWVFGWEGVLLSDRAFFLDYRLAQYRDSWGSFLNQSTEATSQIYLSGVGNRSYFDLR
AIHYLGWASADIQGQIPVIHPVLDYSKTLDRNIFGGEVSFKTNFTSLSRQTAQFDPITTIANTTSLCLNTSADPAARMPS
SCLLRGIPGTYTRATAEAQWRRSFTDPYGQIWTPFASIRMDAIDSSVSNQPGVSNYLPVGDTQAFRVMPTIGLEYRYPFI
NVQPWGTTTIEPIAQVIVRPNETYAGKLPNEDAQSMVFDTSNLFSVDKFSGYDRVEGGGRANVGVQATTQFDRGGAINVL
FGQSYQLFGQNSYAVRDTTNTGLDSGLATARSDYVGRVSYSPNSTYKFTTRARLDEATLDVNRFEAEASASFNRWSVSVI
YGNYAAQPDLGFLTRRQGILTTGSIKVASNWVVSGGARWDLEANRINQYIVGAGYVDDCFVMAVNYVTGYSYANYGTTPT
LNHSVMLQIGLRTIGMGAMQQSVSGASSGVFGQ

Sequences:

>Translated_833_residues
MIAALRDELFALPRRTIVRSKRSLMASCAIPLAALLLAGAADLASISSAEAQSYTYNPRPARPRPPQTGTDGQMLVQATE
VNYDYNNQRVSAVGNVQMFFNGTSVEADRLVYDQNTKRLRAEGNVRMTDATGKITYANMLDLSDDYRDGFVDSLRVDTAE
DTRIAASRADRTDGDYNVFQNGVYTACAPCRDDPKKPPLWQVKGARIIHDQVDKMLYFENAQLEFFGVPMAYLPYFSTPD
PTVKRKTGFLMPFYTTNTTFGMGFEIPFYWALAPDYDVTLTPRITTKQGVLMQGEFRQRLMDGAYQIRAYGISQSDPAAF
GSAPGNRSLRGGVDTKGEFALNDKWVFGWEGVLLSDRAFFLDYRLAQYRDSWGSFLNQSTEATSQIYLSGVGNRSYFDLR
AIHYLGWASADIQGQIPVIHPVLDYSKTLDRNIFGGEVSFKTNFTSLSRQTAQFDPITTIANTTSLCLNTSADPAARMPS
SCLLRGIPGTYTRATAEAQWRRSFTDPYGQIWTPFASIRMDAIDSSVSNQPGVSNYLPVGDTQAFRVMPTIGLEYRYPFI
NVQPWGTTTIEPIAQVIVRPNETYAGKLPNEDAQSMVFDTSNLFSVDKFSGYDRVEGGGRANVGVQATTQFDRGGAINVL
FGQSYQLFGQNSYAVRDTTNTGLDSGLATARSDYVGRVSYSPNSTYKFTTRARLDEATLDVNRFEAEASASFNRWSVSVI
YGNYAAQPDLGFLTRRQGILTTGSIKVASNWVVSGGARWDLEANRINQYIVGAGYVDDCFVMAVNYVTGYSYANYGTTPT
LNHSVMLQIGLRTIGMGAMQQSVSGASSGVFGQ
>Mature_833_residues
MIAALRDELFALPRRTIVRSKRSLMASCAIPLAALLLAGAADLASISSAEAQSYTYNPRPARPRPPQTGTDGQMLVQATE
VNYDYNNQRVSAVGNVQMFFNGTSVEADRLVYDQNTKRLRAEGNVRMTDATGKITYANMLDLSDDYRDGFVDSLRVDTAE
DTRIAASRADRTDGDYNVFQNGVYTACAPCRDDPKKPPLWQVKGARIIHDQVDKMLYFENAQLEFFGVPMAYLPYFSTPD
PTVKRKTGFLMPFYTTNTTFGMGFEIPFYWALAPDYDVTLTPRITTKQGVLMQGEFRQRLMDGAYQIRAYGISQSDPAAF
GSAPGNRSLRGGVDTKGEFALNDKWVFGWEGVLLSDRAFFLDYRLAQYRDSWGSFLNQSTEATSQIYLSGVGNRSYFDLR
AIHYLGWASADIQGQIPVIHPVLDYSKTLDRNIFGGEVSFKTNFTSLSRQTAQFDPITTIANTTSLCLNTSADPAARMPS
SCLLRGIPGTYTRATAEAQWRRSFTDPYGQIWTPFASIRMDAIDSSVSNQPGVSNYLPVGDTQAFRVMPTIGLEYRYPFI
NVQPWGTTTIEPIAQVIVRPNETYAGKLPNEDAQSMVFDTSNLFSVDKFSGYDRVEGGGRANVGVQATTQFDRGGAINVL
FGQSYQLFGQNSYAVRDTTNTGLDSGLATARSDYVGRVSYSPNSTYKFTTRARLDEATLDVNRFEAEASASFNRWSVSVI
YGNYAAQPDLGFLTRRQGILTTGSIKVASNWVVSGGARWDLEANRINQYIVGAGYVDDCFVMAVNYVTGYSYANYGTTPT
LNHSVMLQIGLRTIGMGAMQQSVSGASSGVFGQ

Specific function: Together with lptE, is involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=336, Percent_Identity=27.3809523809524, Blast_Score=93, Evalue=9e-20,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 92024; Mature: 92024

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAALRDELFALPRRTIVRSKRSLMASCAIPLAALLLAGAADLASISSAEAQSYTYNPRP
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCEECCCCC
ARPRPPQTGTDGQMLVQATEVNYDYNNQRVSAVGNVQMFFNGTSVEADRLVYDQNTKRLR
CCCCCCCCCCCCEEEEEEEEECCCCCCCEEEEEEEEEEEEECCCCCCCEEEECCCCCEEE
AEGNVRMTDATGKITYANMLDLSDDYRDGFVDSLRVDTAEDTRIAASRADRTDGDYNVFQ
CCCCEEEECCCCEEEEEEEECCCCCHHCCCHHHEEECCCCCCEEEHHCCCCCCCCHHHHH
NGVYTACAPCRDDPKKPPLWQVKGARIIHDQVDKMLYFENAQLEFFGVPMAYLPYFSTPD
CCCEEEECCCCCCCCCCCEEEECCCEEEHHHHHHEEEEECCCEEEEECCHHHCCCCCCCC
PTVKRKTGFLMPFYTTNTTFGMGFEIPFYWALAPDYDVTLTPRITTKQGVLMQGEFRQRL
CCCCCCCCEEEEEEECCCEECCCCCCCEEEEECCCCCEEECCCEECCCCEEECCHHHHHH
MDGAYQIRAYGISQSDPAAFGSAPGNRSLRGGVDTKGEFALNDKWVFGWEGVLLSDRAFF
HCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCEEEEEECCEEECCCEEE
LDYRLAQYRDSWGSFLNQSTEATSQIYLSGVGNRSYFDLRAIHYLGWASADIQGQIPVIH
EHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEEEEECCCCCCCCCCCCEEE
PVLDYSKTLDRNIFGGEVSFKTNFTSLSRQTAQFDPITTIANTTSLCLNTSADPAARMPS
HHHHHHHHHCCCCCCCEEEEEECHHHHHCCCCCCCCEEECCCCEEEEEECCCCHHHHCCH
SCLLRGIPGTYTRATAEAQWRRSFTDPYGQIWTPFASIRMDAIDSSVSNQPGVSNYLPVG
HHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHCCHHHEEHHHHHCCCCCCCCCCCCCCCC
DTQAFRVMPTIGLEYRYPFINVQPWGTTTIEPIAQVIVRPNETYAGKLPNEDAQSMVFDT
CCCEEEEEEECCCEEECCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHEEEEC
SNLFSVDKFSGYDRVEGGGRANVGVQATTQFDRGGAINVLFGQSYQLFGQNSYAVRDTTN
CCCEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCCEEEECCCCEEEEECCC
TGLDSGLATARSDYVGRVSYSPNSTYKFTTRARLDEATLDVNRFEAEASASFNRWSVSVI
CCCCCCHHHHCCCCEEEEEECCCCCEEEEECCCCCHHHCCHHHHHHHHCCCCCEEEEEEE
YGNYAAQPDLGFLTRRQGILTTGSIKVASNWVVSGGARWDLEANRINQYIVGAGYVDDCF
ECCCCCCCCCCHHHHCCCEEEECCEEEECCEEEECCEEEEEEHHCCCEEEEECCCHHHHH
VMAVNYVTGYSYANYGTTPTLNHSVMLQIGLRTIGMGAMQQSVSGASSGVFGQ
HHHHHHHHCCCCCCCCCCCCCCCEEEEEECCHHHCCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MIAALRDELFALPRRTIVRSKRSLMASCAIPLAALLLAGAADLASISSAEAQSYTYNPRP
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCEECCCCC
ARPRPPQTGTDGQMLVQATEVNYDYNNQRVSAVGNVQMFFNGTSVEADRLVYDQNTKRLR
CCCCCCCCCCCCEEEEEEEEECCCCCCCEEEEEEEEEEEEECCCCCCCEEEECCCCCEEE
AEGNVRMTDATGKITYANMLDLSDDYRDGFVDSLRVDTAEDTRIAASRADRTDGDYNVFQ
CCCCEEEECCCCEEEEEEEECCCCCHHCCCHHHEEECCCCCCEEEHHCCCCCCCCHHHHH
NGVYTACAPCRDDPKKPPLWQVKGARIIHDQVDKMLYFENAQLEFFGVPMAYLPYFSTPD
CCCEEEECCCCCCCCCCCEEEECCCEEEHHHHHHEEEEECCCEEEEECCHHHCCCCCCCC
PTVKRKTGFLMPFYTTNTTFGMGFEIPFYWALAPDYDVTLTPRITTKQGVLMQGEFRQRL
CCCCCCCCEEEEEEECCCEECCCCCCCEEEEECCCCCEEECCCEECCCCEEECCHHHHHH
MDGAYQIRAYGISQSDPAAFGSAPGNRSLRGGVDTKGEFALNDKWVFGWEGVLLSDRAFF
HCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCEEEEEECCEEECCCEEE
LDYRLAQYRDSWGSFLNQSTEATSQIYLSGVGNRSYFDLRAIHYLGWASADIQGQIPVIH
EHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEEEEECCCCCCCCCCCCEEE
PVLDYSKTLDRNIFGGEVSFKTNFTSLSRQTAQFDPITTIANTTSLCLNTSADPAARMPS
HHHHHHHHHCCCCCCCEEEEEECHHHHHCCCCCCCCEEECCCCEEEEEECCCCHHHHCCH
SCLLRGIPGTYTRATAEAQWRRSFTDPYGQIWTPFASIRMDAIDSSVSNQPGVSNYLPVG
HHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHCCHHHEEHHHHHCCCCCCCCCCCCCCCC
DTQAFRVMPTIGLEYRYPFINVQPWGTTTIEPIAQVIVRPNETYAGKLPNEDAQSMVFDT
CCCEEEEEEECCCEEECCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHEEEEC
SNLFSVDKFSGYDRVEGGGRANVGVQATTQFDRGGAINVLFGQSYQLFGQNSYAVRDTTN
CCCEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCCEEEECCCCEEEEECCC
TGLDSGLATARSDYVGRVSYSPNSTYKFTTRARLDEATLDVNRFEAEASASFNRWSVSVI
CCCCCCHHHHCCCCEEEEEECCCCCEEEEECCCCCHHHCCHHHHHHHHCCCCCEEEEEEE
YGNYAAQPDLGFLTRRQGILTTGSIKVASNWVVSGGARWDLEANRINQYIVGAGYVDDCF
ECCCCCCCCCCHHHHCCCEEEECCEEEECCEEEECCEEEEEEHHCCCEEEEECCCHHHHH
VMAVNYVTGYSYANYGTTPTLNHSVMLQIGLRTIGMGAMQQSVSGASSGVFGQ
HHHHHHHHCCCCCCCCCCCCCCCEEEEEECCHHHCCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA