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The map label for this gene is dnaB [H]

Identifier: 86749578

GI number: 86749578

Start: 2814512

End: 2816008

Strand: Reverse

Name: dnaB [H]

Synonym: RPB_2458

Alternate gene names: 86749578

Gene position: 2816008-2814512 (Counterclockwise)

Preceding gene: 86749581

Following gene: 86749577

Centisome position: 52.82

GC content: 64.13

Gene sequence:

>1497_bases
ATGGCCGCCATTGATTCGAACGTTCTGAAGCTCGCGCCCGACGCCACCGCGCCTGCCTATCGGACCGCGCCCCACAATAT
CGAGGCGGAACAGGCCCTGCTCGGCGCCATCCTTGTCAATAATGACGCGTTCTACCGGGTGTCGGACTTTCTCGAGGCGA
AGCACTTCTTCGAAACCATCCACCAGACCATCTTCGAAACGGCGACCAGCCTGATCCGCGCCGGCAAGGTCGCGACGCCG
ATCACGCTCAAAACCTTCCTCGCCGCCGACCTCGATCTCGGCGGCCTGACGGTCGCACAATATCTGGCGCGACTCGCCGC
CGAAGCCACCACCATCATCAACGCCCACGACTACGGCCGGACCATCTACGAGCTGTCGCTGCGGCGCGACCTGATCGGCA
TCGGCACCGATATGGTCAATGTCGCGTTCGACGCGCCGGTCGATTTTGCGCCGCGCAACCAGATCGAGGACGCCGAGCGC
AGGCTGTACGAGCTCGCGGAATCCGGCCGCTATGACGGCGGCTTCCAGCGTTTTTCGCAGGCGCTGACCACCGCGGTCGA
TATGGCCGCAAAGGCGTTCCAGCGCGACGGCAAGCTGTCCGGCATCGCCACCGGCCTGCGCGATCTCGACACCAGGATGG
GCGGACTGCAGCATTCCGACTTGATCGTCCTCGCCGGCCGCCCCGGCATGGGCAAGACCGCGCTCGCCACCAACATCGCC
TACAATGTCGCCAAGGCCTATCGCGGCGAGGTCCAGGCCGACGGCACGACGAAGGCCGTCAATGGCGGCATCGTCGGCTT
CTTCTCCTGCGAAATGTCGGCCGAACAGCTCGCCACCCGTATTCTCGCCGAGCAGGCGGAGATCGCGTCGAGCAAGATCC
GGCGCGGCGGCATTTCGGAATCCGATTTCGAGAGAATCCGCGACGTCTCGATCGAGTTGCAATCGCTGCCGCTTTACGTC
GACGAAACCGGCGGCCTGGCGATCTCTCAACTCACCGCGCGAGCGCGGCGGCTGAAGCGGCAGAAGGGCCTCGACCTGCT
GGTGGTCGACTACATCCAGCTGCTGCAGGGCTCGAGCAAGAAGGGCGACAACCGCGTTCAGGAAGTCACCGAGATCACCA
CCAATCTGAAGGCGCTGGCGAAGGAGCTCAACGTTCCGATCATCGCGCTGTCGCAGCTGTCGCGTCAGGTCGAATCCCGC
GACGACAAGCGTCCCCAGCTTTCCGACCTGCGTGAATCGGGCTCGATCGAACAGGACGCCGACGTGGTGATGTTCGTGTT
CCGCGAGGAGTACTACCTGCAGAACAAGGAGCCACGGCCCGGCACGCCGGAACACGAAAAATGGGCGACCGACATGGACC
TCGTCCACGGCAAGGCCGAAGTCATCATCGCCAAGCAGCGCCATGGCCCGACCGGCACGGTCGACCTGCAGTTCGAGGGC
CAGTTCACGCGCTTCAGCGATCTGGTCAGCGAAGGCCACCTGCCGGAACGGATCTGA

Upstream 100 bases:

>100_bases
GAGCTGGTGCATAACGCGCGATTGACGCTTCCGCTCGCGATTGAATCGGCGCTTTAGTCGCGCCCCGCATCCGACAAATT
CGAAAGAACAATCCAGAGCT

Downstream 100 bases:

>100_bases
GCTGCAGCAACTCCCGTTGAATGGAATTCGGGCAGCGATTATTGTGCGGCATGAACATCCTCCCCGATCCGAATGCGACC
GTCGCCAGCCTGTTGACACC

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MAAIDSNVLKLAPDATAPAYRTAPHNIEAEQALLGAILVNNDAFYRVSDFLEAKHFFETIHQTIFETATSLIRAGKVATP
ITLKTFLAADLDLGGLTVAQYLARLAAEATTIINAHDYGRTIYELSLRRDLIGIGTDMVNVAFDAPVDFAPRNQIEDAER
RLYELAESGRYDGGFQRFSQALTTAVDMAAKAFQRDGKLSGIATGLRDLDTRMGGLQHSDLIVLAGRPGMGKTALATNIA
YNVAKAYRGEVQADGTTKAVNGGIVGFFSCEMSAEQLATRILAEQAEIASSKIRRGGISESDFERIRDVSIELQSLPLYV
DETGGLAISQLTARARRLKRQKGLDLLVVDYIQLLQGSSKKGDNRVQEVTEITTNLKALAKELNVPIIALSQLSRQVESR
DDKRPQLSDLRESGSIEQDADVVMFVFREEYYLQNKEPRPGTPEHEKWATDMDLVHGKAEVIIAKQRHGPTGTVDLQFEG
QFTRFSDLVSEGHLPERI

Sequences:

>Translated_498_residues
MAAIDSNVLKLAPDATAPAYRTAPHNIEAEQALLGAILVNNDAFYRVSDFLEAKHFFETIHQTIFETATSLIRAGKVATP
ITLKTFLAADLDLGGLTVAQYLARLAAEATTIINAHDYGRTIYELSLRRDLIGIGTDMVNVAFDAPVDFAPRNQIEDAER
RLYELAESGRYDGGFQRFSQALTTAVDMAAKAFQRDGKLSGIATGLRDLDTRMGGLQHSDLIVLAGRPGMGKTALATNIA
YNVAKAYRGEVQADGTTKAVNGGIVGFFSCEMSAEQLATRILAEQAEIASSKIRRGGISESDFERIRDVSIELQSLPLYV
DETGGLAISQLTARARRLKRQKGLDLLVVDYIQLLQGSSKKGDNRVQEVTEITTNLKALAKELNVPIIALSQLSRQVESR
DDKRPQLSDLRESGSIEQDADVVMFVFREEYYLQNKEPRPGTPEHEKWATDMDLVHGKAEVIIAKQRHGPTGTVDLQFEG
QFTRFSDLVSEGHLPERI
>Mature_497_residues
AAIDSNVLKLAPDATAPAYRTAPHNIEAEQALLGAILVNNDAFYRVSDFLEAKHFFETIHQTIFETATSLIRAGKVATPI
TLKTFLAADLDLGGLTVAQYLARLAAEATTIINAHDYGRTIYELSLRRDLIGIGTDMVNVAFDAPVDFAPRNQIEDAERR
LYELAESGRYDGGFQRFSQALTTAVDMAAKAFQRDGKLSGIATGLRDLDTRMGGLQHSDLIVLAGRPGMGKTALATNIAY
NVAKAYRGEVQADGTTKAVNGGIVGFFSCEMSAEQLATRILAEQAEIASSKIRRGGISESDFERIRDVSIELQSLPLYVD
ETGGLAISQLTARARRLKRQKGLDLLVVDYIQLLQGSSKKGDNRVQEVTEITTNLKALAKELNVPIIALSQLSRQVESRD
DKRPQLSDLRESGSIEQDADVVMFVFREEYYLQNKEPRPGTPEHEKWATDMDLVHGKAEVIIAKQRHGPTGTVDLQFEGQ
FTRFSDLVSEGHLPERI

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=479, Percent_Identity=36.5344467640919, Blast_Score=288, Evalue=4e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 54744; Mature: 54612

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAIDSNVLKLAPDATAPAYRTAPHNIEAEQALLGAILVNNDAFYRVSDFLEAKHFFETI
CCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHH
HQTIFETATSLIRAGKVATPITLKTFLAADLDLGGLTVAQYLARLAAEATTIINAHDYGR
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEECHHHCC
TIYELSLRRDLIGIGTDMVNVAFDAPVDFAPRNQIEDAERRLYELAESGRYDGGFQRFSQ
HHHHHHHHHHHHHCCHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHH
ALTTAVDMAAKAFQRDGKLSGIATGLRDLDTRMGGLQHSDLIVLAGRPGMGKTALATNIA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHH
YNVAKAYRGEVQADGTTKAVNGGIVGFFSCEMSAEQLATRILAEQAEIASSKIRRGGISE
HHHHHHHCCCCCCCCCCEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCH
SDFERIRDVSIELQSLPLYVDETGGLAISQLTARARRLKRQKGLDLLVVDYIQLLQGSSK
HHHHHHHHHHEEEEECCEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCC
KGDNRVQEVTEITTNLKALAKELNVPIIALSQLSRQVESRDDKRPQLSDLRESGSIEQDA
CCCHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCC
DVVMFVFREEYYLQNKEPRPGTPEHEKWATDMDLVHGKAEVIIAKQRHGPTGTVDLQFEG
CEEEEEEHHHHHHCCCCCCCCCCCHHHHCCCHHHHCCCEEEEEEECCCCCCCEEEEEECC
QFTRFSDLVSEGHLPERI
CHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AAIDSNVLKLAPDATAPAYRTAPHNIEAEQALLGAILVNNDAFYRVSDFLEAKHFFETI
CCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHH
HQTIFETATSLIRAGKVATPITLKTFLAADLDLGGLTVAQYLARLAAEATTIINAHDYGR
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEECHHHCC
TIYELSLRRDLIGIGTDMVNVAFDAPVDFAPRNQIEDAERRLYELAESGRYDGGFQRFSQ
HHHHHHHHHHHHHCCHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHH
ALTTAVDMAAKAFQRDGKLSGIATGLRDLDTRMGGLQHSDLIVLAGRPGMGKTALATNIA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHH
YNVAKAYRGEVQADGTTKAVNGGIVGFFSCEMSAEQLATRILAEQAEIASSKIRRGGISE
HHHHHHHCCCCCCCCCCEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCH
SDFERIRDVSIELQSLPLYVDETGGLAISQLTARARRLKRQKGLDLLVVDYIQLLQGSSK
HHHHHHHHHHEEEEECCEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCC
KGDNRVQEVTEITTNLKALAKELNVPIIALSQLSRQVESRDDKRPQLSDLRESGSIEQDA
CCCHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCC
DVVMFVFREEYYLQNKEPRPGTPEHEKWATDMDLVHGKAEVIIAKQRHGPTGTVDLQFEG
CEEEEEEHHHHHHCCCCCCCCCCCHHHHCCCHHHHCCCEEEEEEECCCCCCCEEEEEECC
QFTRFSDLVSEGHLPERI
CHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA