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The map label for this gene is radA [H]

Identifier: 86749574

GI number: 86749574

Start: 2810662

End: 2812113

Strand: Reverse

Name: radA [H]

Synonym: RPB_2454

Alternate gene names: 86749574

Gene position: 2812113-2810662 (Counterclockwise)

Preceding gene: 86749577

Following gene: 86749573

Centisome position: 52.74

GC content: 70.25

Gene sequence:

>1452_bases
ATGGCCAAAGCCTCGCCCTCCTTCGTCTGCCAGAACTGCGGCGCAGCCTTTAACCGCTGGCAGGGCAAGTGCGAGTCCTG
CGGCGAGTGGAATACGCTGGTCGAGGAAGCCGGCGACAGCGCCGTCCCGGTCAGCATCCGCGCCAAGCGGCGCGGGCGGC
CGTTCAAGCTGGAATCGCTCGCCGGCAAAAGCGTCGACGCGCCGCGCCTGCCCTCCGGCATGGCCGAACTCGACCGCGTC
ACCGGCGGCGGCTTCGTCCGCGGCTCGGTGCTGCTGGTCGGCGGCGATCCCGGCATCGGCAAGTCGACGCTGCTGACCCA
GGCCACCAGCCTGATGGCGCGCAGCGGCCATCGCGCGGTGTACATCTCCGGCGAAGAAGCGGTGGCGCAGGTGCGGCTGC
GCGCCGAGCGGCTCGGCCTCGCCTCCGCGCCGGTCGAACTCGCCGCCGAGACTTCGGTCGAAGACATCATCGCAACCCTT
TCTGAAGGCGCGACGCCGCGGCTGATCGTGATCGATTCGATCCAGACGATGTGGACCGACACGGTCGAGTCGGCGCCCGG
CACGGTGACGCAAGTGCGCGCCTCGGCGCAGGCGCTGATCCGCTTTGCCAAGAAGAGCGGCGCGGCGATCATCCTGGTCG
GCCATGTCACCAAGGACGGCCAGATCGCCGGTCCGCGCGTGGTCGAGCACATGGTCGACGCGGTGCTGTCGTTCGAGGGC
GAAGGCTCGCAGCAGTTCCGCATCCTGCGCGCGGTGAAGAACCGCTTCGGACCGACCGACGAGATCGGCGTGTTCGAGAT
GACCGGGCTCGGCCTGCGCGAAGTCGCCAACCCGTCGGAGCTGTTCCTGTCCGAGCGCGATCTCGGCTCGCCCGGCACCG
CGGTGTTCGCCGGCATCGAGGGCACCCGCCCGGTGCTGGTCGAATTGCAGGCGCTGGTGGCGCCGACCACGCTCGGCACC
CCGCGGCGCGCGGTGGTCGGCTGGGATTCGGCGCGGCTGTCGATGGTGCTGGCGGTGCTGGAGGCGCATTGCGGCGTCAA
GCTGTCGGGCTACGACGTCTATTTCAACGTTGCCGGCGGGCTGCGGATCAACGAGCCGGCGGCCGATCTCGCCGCAGCAG
CAGCGTTGGTGTCGTCGCTGGCGAATTCACCGTTGCCGACCGACGCGGTCTATTTCGGCGAAATCTCGCTGTCGGGCGCG
GTGCGGCCGGTGGCGCAGACCGCCGCTAGGCTAAAGGAAGCCGCCAAGCTCGGCTTTGCCCGCGCCATCTTGCCGGAGAC
CGCCCGCGGCGACGCGCCGTCCGACGCCGGGATGGCGCTGCAGACGGTCGGCGGCCTCACCAGCTTGGTCGCCGACATCG
CCGCGCGTGGAACGCCCCGCCCCGCCGGCCGCGACACTGCGTCTTCGGAGAAAAATGCCACACCGGCGCGATTCCGGCGG
CAGGAAGGCTGA

Upstream 100 bases:

>100_bases
CGCTCGCCAACTGCTGGCGTCAACCTCGCCTCCGAGGTTGACGCCAAGAACAAAGTATGAACAAAAATGTCGACACGCTC
TCGTCGCGACTGCATGAACA

Downstream 100 bases:

>100_bases
ACCGGGGTGACCTTTGCCGGTGGCGCCGCTATACAACGCGCGCGCTTTCCGGCAGCGGCGGCCACCAAGCTTTAACCCAG
CGGAACATTGCTGTGGCGAC

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MAKASPSFVCQNCGAAFNRWQGKCESCGEWNTLVEEAGDSAVPVSIRAKRRGRPFKLESLAGKSVDAPRLPSGMAELDRV
TGGGFVRGSVLLVGGDPGIGKSTLLTQATSLMARSGHRAVYISGEEAVAQVRLRAERLGLASAPVELAAETSVEDIIATL
SEGATPRLIVIDSIQTMWTDTVESAPGTVTQVRASAQALIRFAKKSGAAIILVGHVTKDGQIAGPRVVEHMVDAVLSFEG
EGSQQFRILRAVKNRFGPTDEIGVFEMTGLGLREVANPSELFLSERDLGSPGTAVFAGIEGTRPVLVELQALVAPTTLGT
PRRAVVGWDSARLSMVLAVLEAHCGVKLSGYDVYFNVAGGLRINEPAADLAAAAALVSSLANSPLPTDAVYFGEISLSGA
VRPVAQTAARLKEAAKLGFARAILPETARGDAPSDAGMALQTVGGLTSLVADIAARGTPRPAGRDTASSEKNATPARFRR
QEG

Sequences:

>Translated_483_residues
MAKASPSFVCQNCGAAFNRWQGKCESCGEWNTLVEEAGDSAVPVSIRAKRRGRPFKLESLAGKSVDAPRLPSGMAELDRV
TGGGFVRGSVLLVGGDPGIGKSTLLTQATSLMARSGHRAVYISGEEAVAQVRLRAERLGLASAPVELAAETSVEDIIATL
SEGATPRLIVIDSIQTMWTDTVESAPGTVTQVRASAQALIRFAKKSGAAIILVGHVTKDGQIAGPRVVEHMVDAVLSFEG
EGSQQFRILRAVKNRFGPTDEIGVFEMTGLGLREVANPSELFLSERDLGSPGTAVFAGIEGTRPVLVELQALVAPTTLGT
PRRAVVGWDSARLSMVLAVLEAHCGVKLSGYDVYFNVAGGLRINEPAADLAAAAALVSSLANSPLPTDAVYFGEISLSGA
VRPVAQTAARLKEAAKLGFARAILPETARGDAPSDAGMALQTVGGLTSLVADIAARGTPRPAGRDTASSEKNATPARFRR
QEG
>Mature_482_residues
AKASPSFVCQNCGAAFNRWQGKCESCGEWNTLVEEAGDSAVPVSIRAKRRGRPFKLESLAGKSVDAPRLPSGMAELDRVT
GGGFVRGSVLLVGGDPGIGKSTLLTQATSLMARSGHRAVYISGEEAVAQVRLRAERLGLASAPVELAAETSVEDIIATLS
EGATPRLIVIDSIQTMWTDTVESAPGTVTQVRASAQALIRFAKKSGAAIILVGHVTKDGQIAGPRVVEHMVDAVLSFEGE
GSQQFRILRAVKNRFGPTDEIGVFEMTGLGLREVANPSELFLSERDLGSPGTAVFAGIEGTRPVLVELQALVAPTTLGTP
RRAVVGWDSARLSMVLAVLEAHCGVKLSGYDVYFNVAGGLRINEPAADLAAAAALVSSLANSPLPTDAVYFGEISLSGAV
RPVAQTAARLKEAAKLGFARAILPETARGDAPSDAGMALQTVGGLTSLVADIAARGTPRPAGRDTASSEKNATPARFRRQ
EG

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=452, Percent_Identity=46.6814159292035, Blast_Score=399, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR020588
- InterPro:   IPR004504
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC [H]

EC number: NA

Molecular weight: Translated: 50335; Mature: 50204

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKASPSFVCQNCGAAFNRWQGKCESCGEWNTLVEEAGDSAVPVSIRAKRRGRPFKLESL
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCEEEEEHHCCCCCEEEHHH
AGKSVDAPRLPSGMAELDRVTGGGFVRGSVLLVGGDPGIGKSTLLTQATSLMARSGHRAV
CCCCCCCCCCCCCHHHHHHHCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEE
YISGEEAVAQVRLRAERLGLASAPVELAAETSVEDIIATLSEGATPRLIVIDSIQTMWTD
EECCHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHCCCCCEEEEECCHHHHHHH
TVESAPGTVTQVRASAQALIRFAKKSGAAIILVGHVTKDGQIAGPRVVEHMVDAVLSFEG
HHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCC
EGSQQFRILRAVKNRFGPTDEIGVFEMTGLGLREVANPSELFLSERDLGSPGTAVFAGIE
CCHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHCCHHHHHEECCCCCCCCCEEEECCC
GTRPVLVELQALVAPTTLGTPRRAVVGWDSARLSMVLAVLEAHCGVKLSGYDVYFNVAGG
CCCHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEECEEEEEEECCC
LRINEPAADLAAAAALVSSLANSPLPTDAVYFGEISLSGAVRPVAQTAARLKEAAKLGFA
CEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
RAILPETARGDAPSDAGMALQTVGGLTSLVADIAARGTPRPAGRDTASSEKNATPARFRR
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHC
QEG
CCC
>Mature Secondary Structure 
AKASPSFVCQNCGAAFNRWQGKCESCGEWNTLVEEAGDSAVPVSIRAKRRGRPFKLESL
CCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCEEEEEHHCCCCCEEEHHH
AGKSVDAPRLPSGMAELDRVTGGGFVRGSVLLVGGDPGIGKSTLLTQATSLMARSGHRAV
CCCCCCCCCCCCCHHHHHHHCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEE
YISGEEAVAQVRLRAERLGLASAPVELAAETSVEDIIATLSEGATPRLIVIDSIQTMWTD
EECCHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHCCCCCEEEEECCHHHHHHH
TVESAPGTVTQVRASAQALIRFAKKSGAAIILVGHVTKDGQIAGPRVVEHMVDAVLSFEG
HHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCC
EGSQQFRILRAVKNRFGPTDEIGVFEMTGLGLREVANPSELFLSERDLGSPGTAVFAGIE
CCHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHCCHHHHHEECCCCCCCCCEEEECCC
GTRPVLVELQALVAPTTLGTPRRAVVGWDSARLSMVLAVLEAHCGVKLSGYDVYFNVAGG
CCCHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEECEEEEEEECCC
LRINEPAADLAAAAALVSSLANSPLPTDAVYFGEISLSGAVRPVAQTAARLKEAAKLGFA
CEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
RAILPETARGDAPSDAGMALQTVGGLTSLVADIAARGTPRPAGRDTASSEKNATPARFRR
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHC
QEG
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA