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The map label for this gene is tetA [H]

Identifier: 86749569

GI number: 86749569

Start: 2804705

End: 2805979

Strand: Direct

Name: tetA [H]

Synonym: RPB_2449

Alternate gene names: 86749569

Gene position: 2804705-2805979 (Clockwise)

Preceding gene: 86749568

Following gene: 86749575

Centisome position: 52.6

GC content: 67.61

Gene sequence:

>1275_bases
ATGACCGCGGCCGGCCACGCGAGCGGGACGAGCGCGCCGGCACCCGGCGCGCGCCCCGCCGCGGTCGGCTTCATCTTCGT
CACCATCCTGCTCGACATGCTGAGCGTCGGCATGATCCTGCCGATCCTGCCGAAGCTGATCGAGAGCTTCTCCGACAACG
ACACCGCGGCTGCGGCCAAGATCTACGGCCTGTTCGGCACGGCCTGGGCGCTGATGCAGTTCGTCGCGTCGCCGGTGCTG
GGCGCGCTGTCTGATCGTTTCGGCCGCCGCCGGGTGATCCTGCTGTCCAATCTCGGTCTCGGGCTCGATTACATCCTGAT
GGCGCTGGCGCCGACGCTGGCCTGGCTGTTCATCGGCCGGGTGATCTCCGGCATCACCTCGGCGAGTATCTCAACCTCGT
TCGCCTATATCGCCGACGTCACACCTGCGGAAAAACGCGCGGCCGTGTTCGGCAAGGTCGGCGCCGCCTTCGGCCTCGGC
TTCATCTTCGGCCCGGCGATCGGCGGATTGCTCGGCGGCGTCGATCCGCGGCTGCCGTTCTGGGTGGCGGCCGGCTTGAG
CCTGTGCAACGCGCTGTATGGCTTGTTCGTGCTGCCGGAATCGCTGCCGCCGGAGCGGCGGTCGCCGTTCCGCTGGCGGG
CCGCCAATCCGATCGGCGCGGTGCAGTTGCTGTCGTCGAATGCGATACTCGCCGGTATGGCGATCGTGGCGTTCTGCGCC
GAGGTCGCCCATGTGGCGCTGTCCGCGACCTTCGTCCTCTACGCCAGCTATCGCTACGCGTGGGATCAGACCACGGTCGG
TCTCGCCTTGGCCTTCGTCGGCTTCTGCACTACCGTGGTGCAGGGCTTTCTCGTCGGCCCCGCGGTCAAGCGGCTCGGTG
AGCGGCGCGCCCAAGTGATCGGCTATCTCGGCGGCGCAGCGGGCTTTCTGATCTATGCGCTGGCGCCGACCGGCGCGCTG
TTCTGGATCGGCATTCCGGTGATGACGCTGTGGGGCATCGCTAAGCCGGCGACCGCCGGGGTGATGACGCGCCTGGTGGC
GCCGGCGCAGCAAGGACAATTGCAGGGCGCAACCACCAGCATGAACAGCATCGCGGCGCTGATCGGGCCTTTCCTGTTCA
CGGGCATCTTCGCCTATTTCATCGAGCCCGATGCGCCGATTTGGTTTCCCGGCGCGCCGTTCCTGCTCGCCGGCGCGCTG
CTGATGGTTTCGATGCTACTCGCAGGCATCTCGACGCCGCCGCAAAGCAAATCAGGCGCCGCAAGCGGCGCCTGA

Upstream 100 bases:

>100_bases
TCAACAGTCTGCGCGGCGTGTTCAAACTGCCGGGCACGCCGGTCCGCATCACGCTGCGCGAAAAGGCCAATCCTTTCGCC
CACAAGCGCAAACGGAAATC

Downstream 100 bases:

>100_bases
AAGCTGAAACTCGTGGCGTTCGTCGTCATTGCGAGAAGCGAAGCGACGAAGCAATCCAGCACGGCACTCGGTTATCTGGA
TTGCTCGCAATGACGGCGTT

Product: major facilitator transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: TetA(A) [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MTAAGHASGTSAPAPGARPAAVGFIFVTILLDMLSVGMILPILPKLIESFSDNDTAAAAKIYGLFGTAWALMQFVASPVL
GALSDRFGRRRVILLSNLGLGLDYILMALAPTLAWLFIGRVISGITSASISTSFAYIADVTPAEKRAAVFGKVGAAFGLG
FIFGPAIGGLLGGVDPRLPFWVAAGLSLCNALYGLFVLPESLPPERRSPFRWRAANPIGAVQLLSSNAILAGMAIVAFCA
EVAHVALSATFVLYASYRYAWDQTTVGLALAFVGFCTTVVQGFLVGPAVKRLGERRAQVIGYLGGAAGFLIYALAPTGAL
FWIGIPVMTLWGIAKPATAGVMTRLVAPAQQGQLQGATTSMNSIAALIGPFLFTGIFAYFIEPDAPIWFPGAPFLLAGAL
LMVSMLLAGISTPPQSKSGAASGA

Sequences:

>Translated_424_residues
MTAAGHASGTSAPAPGARPAAVGFIFVTILLDMLSVGMILPILPKLIESFSDNDTAAAAKIYGLFGTAWALMQFVASPVL
GALSDRFGRRRVILLSNLGLGLDYILMALAPTLAWLFIGRVISGITSASISTSFAYIADVTPAEKRAAVFGKVGAAFGLG
FIFGPAIGGLLGGVDPRLPFWVAAGLSLCNALYGLFVLPESLPPERRSPFRWRAANPIGAVQLLSSNAILAGMAIVAFCA
EVAHVALSATFVLYASYRYAWDQTTVGLALAFVGFCTTVVQGFLVGPAVKRLGERRAQVIGYLGGAAGFLIYALAPTGAL
FWIGIPVMTLWGIAKPATAGVMTRLVAPAQQGQLQGATTSMNSIAALIGPFLFTGIFAYFIEPDAPIWFPGAPFLLAGAL
LMVSMLLAGISTPPQSKSGAASGA
>Mature_423_residues
TAAGHASGTSAPAPGARPAAVGFIFVTILLDMLSVGMILPILPKLIESFSDNDTAAAAKIYGLFGTAWALMQFVASPVLG
ALSDRFGRRRVILLSNLGLGLDYILMALAPTLAWLFIGRVISGITSASISTSFAYIADVTPAEKRAAVFGKVGAAFGLGF
IFGPAIGGLLGGVDPRLPFWVAAGLSLCNALYGLFVLPESLPPERRSPFRWRAANPIGAVQLLSSNAILAGMAIVAFCAE
VAHVALSATFVLYASYRYAWDQTTVGLALAFVGFCTTVVQGFLVGPAVKRLGERRAQVIGYLGGAAGFLIYALAPTGALF
WIGIPVMTLWGIAKPATAGVMTRLVAPAQQGQLQGATTSMNSIAALIGPFLFTGIFAYFIEPDAPIWFPGAPFLLAGALL
MVSMLLAGISTPPQSKSGAASGA

Specific function: Resistance to tetracycline by an active tetracycline efflux. This is an energy-dependent process that decreases the accumulation of the antibiotic in whole cells. This protein functions as a metal-tetracycline/H(+) antiporter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Homo sapiens, GI39753965, Length=366, Percent_Identity=27.0491803278689, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI94721311, Length=416, Percent_Identity=26.4423076923077, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI225703104, Length=278, Percent_Identity=27.3381294964029, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI225703102, Length=278, Percent_Identity=27.3381294964029, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI217416366, Length=177, Percent_Identity=32.7683615819209, Blast_Score=73, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71996331, Length=373, Percent_Identity=23.8605898123324, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17551570, Length=418, Percent_Identity=22.488038277512, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI221513297, Length=378, Percent_Identity=27.2486772486773, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21357569, Length=382, Percent_Identity=25.130890052356, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24656463, Length=367, Percent_Identity=25.0681198910082, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24656468, Length=367, Percent_Identity=25.0681198910082, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161080926, Length=367, Percent_Identity=25.0681198910082, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI161080924, Length=367, Percent_Identity=25.0681198910082, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI17738085, Length=414, Percent_Identity=26.8115942028986, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24667557, Length=228, Percent_Identity=28.9473684210526, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24667561, Length=198, Percent_Identity=29.2929292929293, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734
- InterPro:   IPR005829
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43908; Mature: 43777

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAGHASGTSAPAPGARPAAVGFIFVTILLDMLSVGMILPILPKLIESFSDNDTAAAAK
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
IYGLFGTAWALMQFVASPVLGALSDRFGRRRVILLSNLGLGLDYILMALAPTLAWLFIGR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
VISGITSASISTSFAYIADVTPAEKRAAVFGKVGAAFGLGFIFGPAIGGLLGGVDPRLPF
HHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
WVAAGLSLCNALYGLFVLPESLPPERRSPFRWRAANPIGAVQLLSSNAILAGMAIVAFCA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCHHHHHHCCCHHHHHHHHHHHHH
EVAHVALSATFVLYASYRYAWDQTTVGLALAFVGFCTTVVQGFLVGPAVKRLGERRAQVI
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYLGGAAGFLIYALAPTGALFWIGIPVMTLWGIAKPATAGVMTRLVAPAQQGQLQGATTS
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHCCCCCCCHHH
MNSIAALIGPFLFTGIFAYFIEPDAPIWFPGAPFLLAGALLMVSMLLAGISTPPQSKSGA
HHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
ASGA
CCCC
>Mature Secondary Structure 
TAAGHASGTSAPAPGARPAAVGFIFVTILLDMLSVGMILPILPKLIESFSDNDTAAAAK
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
IYGLFGTAWALMQFVASPVLGALSDRFGRRRVILLSNLGLGLDYILMALAPTLAWLFIGR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
VISGITSASISTSFAYIADVTPAEKRAAVFGKVGAAFGLGFIFGPAIGGLLGGVDPRLPF
HHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
WVAAGLSLCNALYGLFVLPESLPPERRSPFRWRAANPIGAVQLLSSNAILAGMAIVAFCA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCHHHHHHCCCHHHHHHHHHHHHH
EVAHVALSATFVLYASYRYAWDQTTVGLALAFVGFCTTVVQGFLVGPAVKRLGERRAQVI
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYLGGAAGFLIYALAPTGALFWIGIPVMTLWGIAKPATAGVMTRLVAPAQQGQLQGATTS
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHCCCCCCCHHH
MNSIAALIGPFLFTGIFAYFIEPDAPIWFPGAPFLLAGALLMVSMLLAGISTPPQSKSGA
HHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
ASGA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1312499; 6310527 [H]