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The map label for this gene is gatB

Identifier: 86749560

GI number: 86749560

Start: 2796121

End: 2797605

Strand: Direct

Name: gatB

Synonym: RPB_2440

Alternate gene names: 86749560

Gene position: 2796121-2797605 (Clockwise)

Preceding gene: 86749559

Following gene: 86749561

Centisome position: 52.44

GC content: 64.31

Gene sequence:

>1485_bases
ATGAACGCACCCGTCAAACCGTCCCAGCTCATCAAGGGCGCCACCGGCGATTGGGAAGTCGTGATCGGGCTGGAGATCCA
CGCCCAGGTCTCGTCCAACGCCAAGCTGTTCTCCGGTGCGGCGACCGAATTCGGCGGCGATCCCAACAGCCACGTCTCGC
TGGTCGACGCCGCGATGCCCGGCATGCTGCCGGTGATCAACGAGGAGTGCGTGAAGCAGGCGATCCGTTCGGGACTGGGA
CTGAACGCCCAGATCAACCTGCGCTCGGTGTTCGACCGCAAGAACTATTTCTATCCGGATCTGCCGCAGGGCTACCAGAT
CAGCCAGTACAAGTCGCCGATCGTGGGCGAGGGCGAGGTCGTGGTCGATCTGCCGGACGGCGACAGCACCACCGTCGGCA
TCGAGCGGCTGCATCTCGAACAGGACGCCGGCAAATTGCTGCACGATCAGGACCCGACTTCGACCTTCGTCGATCTCAAT
CGCTCCGGCGTTGCGCTGATGGAGATCGTCTCCAAGCCGGATCTGCGGTCGTCCGAACAGGCCAAGGCCTATGTGTCGAA
GCTGCGCACCATCCTGCGCTATCTCGGCACCTGCGACGGCGACATGGAAAAGGGCAGCCTGCGCGCCGACGTCAACGTCT
CGGTGCGCAAGCCGGGCGAGCCGTACGGCACGCGGTGCGAGATCAAGAACGTCAACTCGATCCGCTTTATCGGTCAGGCG
ATCGAATACGAGGCTCGCCGCCAGATCGGAGTTCTCGAAGACGGCGGCGCGATCGTGCAGGAGACACGGCTGTTCGATGC
GGGCAAGGGCGAGACGCGGTCGATGCGTTCCAAGGAGGAGGCGCACGACTATCGCTACTTCCCCGATCCAGACCTGCTGC
CGCTGGAATTCTCCCAGGCCTATGTCGACGAGCTGAAGGCCGGGTTGCCGGAACTGCCGGACCAGAAGAAGGCCCGGTTC
ATCGGCAGTTTCGGGCTCTCGCCGGACGACGCGGGCGTGCTGGTGAGCGAGCGGGAGAGCGCCGATTTCTACGAGGCGGT
GCTCGCCGCACTTGCGGACGCCGCGCGCGACGGCAAGCTCGCGGCCAACTGGGTGATCAACGAACTGTTCGGCCGCCTCA
ACAAGGACGGCCAGAGCATCGAGGTGTCGCCGGTCTCCGCCGCGCAGCTGGCTGCGATCGTCGACCTGATCGGCGAAGGC
ACCATCTCCGGCAAGATCGCCAAAGAGCTGTTCGAAATCGTCTGGACCGAAGGCGGCGATCCGCGCGTACTGGTCGAAGC
GCGCGGCATGAAGCAGGTCACCGACCTCTCCGCGATCGAGAAGGTGGTCGACGAGATCATCGCCGGCAATCCCGACAAGG
TCGCGCAGGCGATCGCCAAGCCGGCGATGCTCGGCTGGTTCGTCGGACAGGTGATGAAATCGTCCGGCGGCAAGGCGAAC
CCGCAGGCCGTGAACGACCTCTTGAAGCGTAAGCTCGGTCTCTGA

Upstream 100 bases:

>100_bases
CGGTCCCCGCATCCATGCTGTTTCGGCTTCGTACTGTTGGAAAATGACGCAGATCGGCCTATTCAGGCCCTCATTGCTGA
ATTCGTCAGAGAGTTTCTCC

Downstream 100 bases:

>100_bases
CGGCGCGCCGCGTGTGCCGTCTTCGCCGTCGTCGCGACGCGCGGAGGCTGCGCGGCGCCGACGATCGCTCTCGAATCATC
GGTTTCGATTCGCGCCGAAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MNAPVKPSQLIKGATGDWEVVIGLEIHAQVSSNAKLFSGAATEFGGDPNSHVSLVDAAMPGMLPVINEECVKQAIRSGLG
LNAQINLRSVFDRKNYFYPDLPQGYQISQYKSPIVGEGEVVVDLPDGDSTTVGIERLHLEQDAGKLLHDQDPTSTFVDLN
RSGVALMEIVSKPDLRSSEQAKAYVSKLRTILRYLGTCDGDMEKGSLRADVNVSVRKPGEPYGTRCEIKNVNSIRFIGQA
IEYEARRQIGVLEDGGAIVQETRLFDAGKGETRSMRSKEEAHDYRYFPDPDLLPLEFSQAYVDELKAGLPELPDQKKARF
IGSFGLSPDDAGVLVSERESADFYEAVLAALADAARDGKLAANWVINELFGRLNKDGQSIEVSPVSAAQLAAIVDLIGEG
TISGKIAKELFEIVWTEGGDPRVLVEARGMKQVTDLSAIEKVVDEIIAGNPDKVAQAIAKPAMLGWFVGQVMKSSGGKAN
PQAVNDLLKRKLGL

Sequences:

>Translated_494_residues
MNAPVKPSQLIKGATGDWEVVIGLEIHAQVSSNAKLFSGAATEFGGDPNSHVSLVDAAMPGMLPVINEECVKQAIRSGLG
LNAQINLRSVFDRKNYFYPDLPQGYQISQYKSPIVGEGEVVVDLPDGDSTTVGIERLHLEQDAGKLLHDQDPTSTFVDLN
RSGVALMEIVSKPDLRSSEQAKAYVSKLRTILRYLGTCDGDMEKGSLRADVNVSVRKPGEPYGTRCEIKNVNSIRFIGQA
IEYEARRQIGVLEDGGAIVQETRLFDAGKGETRSMRSKEEAHDYRYFPDPDLLPLEFSQAYVDELKAGLPELPDQKKARF
IGSFGLSPDDAGVLVSERESADFYEAVLAALADAARDGKLAANWVINELFGRLNKDGQSIEVSPVSAAQLAAIVDLIGEG
TISGKIAKELFEIVWTEGGDPRVLVEARGMKQVTDLSAIEKVVDEIIAGNPDKVAQAIAKPAMLGWFVGQVMKSSGGKAN
PQAVNDLLKRKLGL
>Mature_494_residues
MNAPVKPSQLIKGATGDWEVVIGLEIHAQVSSNAKLFSGAATEFGGDPNSHVSLVDAAMPGMLPVINEECVKQAIRSGLG
LNAQINLRSVFDRKNYFYPDLPQGYQISQYKSPIVGEGEVVVDLPDGDSTTVGIERLHLEQDAGKLLHDQDPTSTFVDLN
RSGVALMEIVSKPDLRSSEQAKAYVSKLRTILRYLGTCDGDMEKGSLRADVNVSVRKPGEPYGTRCEIKNVNSIRFIGQA
IEYEARRQIGVLEDGGAIVQETRLFDAGKGETRSMRSKEEAHDYRYFPDPDLLPLEFSQAYVDELKAGLPELPDQKKARF
IGSFGLSPDDAGVLVSERESADFYEAVLAALADAARDGKLAANWVINELFGRLNKDGQSIEVSPVSAAQLAAIVDLIGEG
TISGKIAKELFEIVWTEGGDPRVLVEARGMKQVTDLSAIEKVVDEIIAGNPDKVAQAIAKPAMLGWFVGQVMKSSGGKAN
PQAVNDLLKRKLGL

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=512, Percent_Identity=39.0625, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI115534094, Length=311, Percent_Identity=41.4790996784566, Blast_Score=222, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6319391, Length=525, Percent_Identity=32.5714285714286, Blast_Score=233, Evalue=4e-62,
Organism=Drosophila melanogaster, GI45550808, Length=494, Percent_Identity=37.0445344129555, Blast_Score=321, Evalue=9e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_RHOP2 (Q2IXB5)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_486056.1
- ProteinModelPortal:   Q2IXB5
- SMR:   Q2IXB5
- STRING:   Q2IXB5
- GeneID:   3910229
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_2440
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   RPAL316058:RPB_2440-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53484; Mature: 53484

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPVKPSQLIKGATGDWEVVIGLEIHAQVSSNAKLFSGAATEFGGDPNSHVSLVDAAMP
CCCCCCHHHHHCCCCCCEEEEEEEEEEEEECCCCEEEECCCHHCCCCCCCCEEEHHHHCC
GMLPVINEECVKQAIRSGLGLNAQINLRSVFDRKNYFYPDLPQGYQISQYKSPIVGEGEV
CCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCEE
VVDLPDGDSTTVGIERLHLEQDAGKLLHDQDPTSTFVDLNRSGVALMEIVSKPDLRSSEQ
EEECCCCCCCEEEHHHEEEHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHCCCCCCCHHH
AKAYVSKLRTILRYLGTCDGDMEKGSLRADVNVSVRKPGEPYGTRCEIKNVNSIRFIGQA
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEECCCCHHHHHHHH
IEYEARRQIGVLEDGGAIVQETRLFDAGKGETRSMRSKEEAHDYRYFPDPDLLPLEFSQA
HHHHHHHCCCEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
YVDELKAGLPELPDQKKARFIGSFGLSPDDAGVLVSERESADFYEAVLAALADAARDGKL
HHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCH
AANWVINELFGRLNKDGQSIEVSPVSAAQLAAIVDLIGEGTISGKIAKELFEIVWTEGGD
HHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
PRVLVEARGMKQVTDLSAIEKVVDEIIAGNPDKVAQAIAKPAMLGWFVGQVMKSSGGKAN
CEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PQAVNDLLKRKLGL
HHHHHHHHHHHHCC
>Mature Secondary Structure
MNAPVKPSQLIKGATGDWEVVIGLEIHAQVSSNAKLFSGAATEFGGDPNSHVSLVDAAMP
CCCCCCHHHHHCCCCCCEEEEEEEEEEEEECCCCEEEECCCHHCCCCCCCCEEEHHHHCC
GMLPVINEECVKQAIRSGLGLNAQINLRSVFDRKNYFYPDLPQGYQISQYKSPIVGEGEV
CCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCEE
VVDLPDGDSTTVGIERLHLEQDAGKLLHDQDPTSTFVDLNRSGVALMEIVSKPDLRSSEQ
EEECCCCCCCEEEHHHEEEHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHCCCCCCCHHH
AKAYVSKLRTILRYLGTCDGDMEKGSLRADVNVSVRKPGEPYGTRCEIKNVNSIRFIGQA
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEECCCCHHHHHHHH
IEYEARRQIGVLEDGGAIVQETRLFDAGKGETRSMRSKEEAHDYRYFPDPDLLPLEFSQA
HHHHHHHCCCEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
YVDELKAGLPELPDQKKARFIGSFGLSPDDAGVLVSERESADFYEAVLAALADAARDGKL
HHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCH
AANWVINELFGRLNKDGQSIEVSPVSAAQLAAIVDLIGEGTISGKIAKELFEIVWTEGGD
HHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
PRVLVEARGMKQVTDLSAIEKVVDEIIAGNPDKVAQAIAKPAMLGWFVGQVMKSSGGKAN
CEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PQAVNDLLKRKLGL
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA