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The map label for this gene is dacF [H]

Identifier: 86749513

GI number: 86749513

Start: 2744538

End: 2746259

Strand: Reverse

Name: dacF [H]

Synonym: RPB_2393

Alternate gene names: 86749513

Gene position: 2746259-2744538 (Counterclockwise)

Preceding gene: 86749514

Following gene: 86749508

Centisome position: 51.51

GC content: 68.06

Gene sequence:

>1722_bases
ATGCACCGCAACACCTATGTCAGCTCGCGGGTCTTGCGAGTATGCGTACTCGGTCTGGCCACCATCGCGGGCGCGGTCAT
CGCCACCACCGACACGGCCGACGCACGGCGAAAACACCGTCGACATCACGTCGAGCGCAACAGCTACAATCCGGCCTTCG
CCTCGATCATCGTCGACGCCAATACCGGCGCCACGCTGAGCGCGACCAATCCCGACGCCGTGCGCCATCCCGCGTCGCTG
ACCAAGATCATGACGCTGTATCTGCTGTTCGAGCGGCTGGAGGCGGGCAAGCTGTCGCTCGACAGCGAGATGGAGGTGTC
GGCGCATGCGTCGTCGCAAGCGCCGACCAAGCTCGGTCTGCGCCCCGGCCAGACGCTGCGCGTCGAAGACGCGATCAAGG
CCCTCGTCACCCGTTCCGCCAACGACGCTGCCGTGGTGATCGCCGAAACCGTCGGCGGCAGCGAAGCCGAGTTTGCCAGG
ATGATGACGCGGAAGGCGCGCGCCCTCGGAATGTCCCGGACCGTCTACCGCAACTCCAACGGCCTGCCGAACGACGACCA
GGTGACTACCGCGCGGGACCAGTCGATCCTCGGCCGCGCGATCCAGGACCGCTTCCCGCGCTATTACCGCTACTTCGCCA
CCACCACGTTCAACTACCGTGGCGCGTCGATCCGAAATCACAATCGCCTGCTCGGCAATGTCGAAGGCGTCGACGGCATC
AAGACCGGCTACACCCGCGCGTCAGGCTTCAACCTCGTCACCTCGATGCACCGCGGCAACCGCTTCCTCGTCGGCGTTGT
GCTGGGCGGTCGCAGCGGTGGTTCGCGCGACGCGATCATGCGCAACCTGCTCGCTGAAAATCTGGAGAAGGGCGCGACAA
GGCGCACCGTCGCTGCGATCGTCGAACGCTCGCCGTCGTCGACGCAGGTCGCAGAAGACATGCCGGACAGCCGCCCATCG
CCGACCGTCCAGGTCCAGGGCGCCGTTCAGGTCGCATCGGCCGCGCCGGAGCCGATCGAGACGGTCGCCCGCCCGGCCCC
GCCCGCGCCGATCACCCGCGCGATCGCAACGCGGCCCGAGAAGCCCGAGCCCGGCCCGTTTAACAGCGGCACGATCGAGA
CCAAGCCGCTGTCGCTGATCCCGGGGTCGTCGGAGCCGATGAAGCCGGTCCGGGTGAAGACCGTTCAGGTCAAATCGGCG
CCGATCAAGCTCGCCTCCGCCGTCGCCGCGCCGCCCGTGACCAACACCATCCCGCCGGCCCGCCGCGAGATCGCCGAAAC
CTCCACCGCCGCCATCACCAGGGCCGAGCTGGTGCAGCAGCCGGCCAATCACGGCACCGGCAATGGCATCCTCGGCGTCC
TGCCGGCATCCCGAGTGGCGCCGTCGAACGCCCAGGCGATGGCCGCGGTCGAGCCGACGCCCGCGCCGACGTCCAGCGCG
GTTCAGCAGAACGGAGCGATCAAGTCGGTGACCCACACCGGCTGGATCATCCAGGTCGGCGCGCTGGAGAGCGAGAGCGA
AGCCAAGGCCCGGCTCGAAGCCGCGCGCGATCAGGCCCGCGGCATGCTCGGCAAGGCCGATCCGTTCACCGAGACGGTGG
TGTCCAAGGGCGATCGCAAGCTGTATCGCGCCCGCTTCGCCGGGCTCGGTCGCGATGAAGCCGAAGCGGTGTGCCGCAAG
CTGAAGCGCTCGGACATCTCCTGCTTCACCATCAAGAACTGA

Upstream 100 bases:

>100_bases
GCGGCTGAACTCGAGGCCGAACCGGGAGGCGTCGCCGCCGTGATGGGCATAATTCTGCCTCACGGTCCGGTGATTGATGA
AAGATCGGACGGGGACGTTC

Downstream 100 bases:

>100_bases
CGCACCTCCGACGCAACGTGATCTACGGCCGGACACGCGTCCGGCCGTTTTCGTTAGTGCACCGTCCTTTGCGACGAAGG
GATCGCAGCCGTGCGCTGCG

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MHRNTYVSSRVLRVCVLGLATIAGAVIATTDTADARRKHRRHHVERNSYNPAFASIIVDANTGATLSATNPDAVRHPASL
TKIMTLYLLFERLEAGKLSLDSEMEVSAHASSQAPTKLGLRPGQTLRVEDAIKALVTRSANDAAVVIAETVGGSEAEFAR
MMTRKARALGMSRTVYRNSNGLPNDDQVTTARDQSILGRAIQDRFPRYYRYFATTTFNYRGASIRNHNRLLGNVEGVDGI
KTGYTRASGFNLVTSMHRGNRFLVGVVLGGRSGGSRDAIMRNLLAENLEKGATRRTVAAIVERSPSSTQVAEDMPDSRPS
PTVQVQGAVQVASAAPEPIETVARPAPPAPITRAIATRPEKPEPGPFNSGTIETKPLSLIPGSSEPMKPVRVKTVQVKSA
PIKLASAVAAPPVTNTIPPARREIAETSTAAITRAELVQQPANHGTGNGILGVLPASRVAPSNAQAMAAVEPTPAPTSSA
VQQNGAIKSVTHTGWIIQVGALESESEAKARLEAARDQARGMLGKADPFTETVVSKGDRKLYRARFAGLGRDEAEAVCRK
LKRSDISCFTIKN

Sequences:

>Translated_573_residues
MHRNTYVSSRVLRVCVLGLATIAGAVIATTDTADARRKHRRHHVERNSYNPAFASIIVDANTGATLSATNPDAVRHPASL
TKIMTLYLLFERLEAGKLSLDSEMEVSAHASSQAPTKLGLRPGQTLRVEDAIKALVTRSANDAAVVIAETVGGSEAEFAR
MMTRKARALGMSRTVYRNSNGLPNDDQVTTARDQSILGRAIQDRFPRYYRYFATTTFNYRGASIRNHNRLLGNVEGVDGI
KTGYTRASGFNLVTSMHRGNRFLVGVVLGGRSGGSRDAIMRNLLAENLEKGATRRTVAAIVERSPSSTQVAEDMPDSRPS
PTVQVQGAVQVASAAPEPIETVARPAPPAPITRAIATRPEKPEPGPFNSGTIETKPLSLIPGSSEPMKPVRVKTVQVKSA
PIKLASAVAAPPVTNTIPPARREIAETSTAAITRAELVQQPANHGTGNGILGVLPASRVAPSNAQAMAAVEPTPAPTSSA
VQQNGAIKSVTHTGWIIQVGALESESEAKARLEAARDQARGMLGKADPFTETVVSKGDRKLYRARFAGLGRDEAEAVCRK
LKRSDISCFTIKN
>Mature_573_residues
MHRNTYVSSRVLRVCVLGLATIAGAVIATTDTADARRKHRRHHVERNSYNPAFASIIVDANTGATLSATNPDAVRHPASL
TKIMTLYLLFERLEAGKLSLDSEMEVSAHASSQAPTKLGLRPGQTLRVEDAIKALVTRSANDAAVVIAETVGGSEAEFAR
MMTRKARALGMSRTVYRNSNGLPNDDQVTTARDQSILGRAIQDRFPRYYRYFATTTFNYRGASIRNHNRLLGNVEGVDGI
KTGYTRASGFNLVTSMHRGNRFLVGVVLGGRSGGSRDAIMRNLLAENLEKGATRRTVAAIVERSPSSTQVAEDMPDSRPS
PTVQVQGAVQVASAAPEPIETVARPAPPAPITRAIATRPEKPEPGPFNSGTIETKPLSLIPGSSEPMKPVRVKTVQVKSA
PIKLASAVAAPPVTNTIPPARREIAETSTAAITRAELVQQPANHGTGNGILGVLPASRVAPSNAQAMAAVEPTPAPTSSA
VQQNGAIKSVTHTGWIIQVGALESESEAKARLEAARDQARGMLGKADPFTETVVSKGDRKLYRARFAGLGRDEAEAVCRK
LKRSDISCFTIKN

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=291, Percent_Identity=31.6151202749141, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1787062, Length=226, Percent_Identity=34.9557522123894, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI87082022, Length=238, Percent_Identity=32.7731092436975, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI87082054, Length=211, Percent_Identity=29.8578199052133, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 61208; Mature: 61208

Theoretical pI: Translated: 10.78; Mature: 10.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRNTYVSSRVLRVCVLGLATIAGAVIATTDTADARRKHRRHHVERNSYNPAFASIIVDA
CCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHHHHCCCCCCCCEEEEEEEEC
NTGATLSATNPDAVRHPASLTKIMTLYLLFERLEAGKLSLDSEMEVSAHASSQAPTKLGL
CCCCEEECCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHCCCCCCCCCCCC
RPGQTLRVEDAIKALVTRSANDAAVVIAETVGGSEAEFARMMTRKARALGMSRTVYRNSN
CCCCEEEHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCHHEEEECCC
GLPNDDQVTTARDQSILGRAIQDRFPRYYRYFATTTFNYRGASIRNHNRLLGNVEGVDGI
CCCCCCCEECHHHHHHHHHHHHHHCHHHHHHHHEEEECCCCCCCCCCCHHCCCCCCCCCC
KTGYTRASGFNLVTSMHRGNRFLVGVVLGGRSGGSRDAIMRNLLAENLEKGATRRTVAAI
CCCCCCCCCCHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHH
VERSPSSTQVAEDMPDSRPSPTVQVQGAVQVASAAPEPIETVARPAPPAPITRAIATRPE
HHCCCCCCHHHHCCCCCCCCCEEEEECEEEECCCCCCHHHHHCCCCCCCHHHHHHHCCCC
KPEPGPFNSGTIETKPLSLIPGSSEPMKPVRVKTVQVKSAPIKLASAVAAPPVTNTIPPA
CCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEEEEEEECCCHHHHHHHCCCCCCCCCCHH
RREIAETSTAAITRAELVQQPANHGTGNGILGVLPASRVAPSNAQAMAAVEPTPAPTSSA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCCHHH
VQQNGAIKSVTHTGWIIQVGALESESEAKARLEAARDQARGMLGKADPFTETVVSKGDRK
HHHCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHH
LYRARFAGLGRDEAEAVCRKLKRSDISCFTIKN
HHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECC
>Mature Secondary Structure
MHRNTYVSSRVLRVCVLGLATIAGAVIATTDTADARRKHRRHHVERNSYNPAFASIIVDA
CCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHHHHCCCCCCCCEEEEEEEEC
NTGATLSATNPDAVRHPASLTKIMTLYLLFERLEAGKLSLDSEMEVSAHASSQAPTKLGL
CCCCEEECCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHCCCCCCCCCCCC
RPGQTLRVEDAIKALVTRSANDAAVVIAETVGGSEAEFARMMTRKARALGMSRTVYRNSN
CCCCEEEHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCHHEEEECCC
GLPNDDQVTTARDQSILGRAIQDRFPRYYRYFATTTFNYRGASIRNHNRLLGNVEGVDGI
CCCCCCCEECHHHHHHHHHHHHHHCHHHHHHHHEEEECCCCCCCCCCCHHCCCCCCCCCC
KTGYTRASGFNLVTSMHRGNRFLVGVVLGGRSGGSRDAIMRNLLAENLEKGATRRTVAAI
CCCCCCCCCCHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHH
VERSPSSTQVAEDMPDSRPSPTVQVQGAVQVASAAPEPIETVARPAPPAPITRAIATRPE
HHCCCCCCHHHHCCCCCCCCCEEEEECEEEECCCCCCHHHHHCCCCCCCHHHHHHHCCCC
KPEPGPFNSGTIETKPLSLIPGSSEPMKPVRVKTVQVKSAPIKLASAVAAPPVTNTIPPA
CCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEEEEEEECCCHHHHHHHCCCCCCCCCCHH
RREIAETSTAAITRAELVQQPANHGTGNGILGVLPASRVAPSNAQAMAAVEPTPAPTSSA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCCHHH
VQQNGAIKSVTHTGWIIQVGALESESEAKARLEAARDQARGMLGKADPFTETVVSKGDRK
HHHCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHH
LYRARFAGLGRDEAEAVCRKLKRSDISCFTIKN
HHHHHHHCCCCHHHHHHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]