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The map label for this gene is rlmB [H]

Identifier: 86749323

GI number: 86749323

Start: 2495058

End: 2495879

Strand: Reverse

Name: rlmB [H]

Synonym: RPB_2203

Alternate gene names: 86749323

Gene position: 2495879-2495058 (Counterclockwise)

Preceding gene: 86749328

Following gene: 86749317

Centisome position: 46.81

GC content: 71.17

Gene sequence:

>822_bases
ATGAGTCCGAAAGAGCGAAATCCGCGGTTCCAGCGCCGCGACGACCGGGACCGTGGCCGACCGCAGGGATCAGGCGGGCG
CCATGGCGCGCGCGAGCGCGATCCCGACGGGCCGGCGATCCTGTATGGCTGGCACACCGTCACCGCCGCGCTGGCCAATC
CCGCGCGCCGCATCCGCAAGCTGCTCCTGACCGAGAACGCGGCGCGCAAACTCGCCGACGAGAACATCAACACCAGGATC
ACACCGGACATCGTGCGTCCCGGCGAGATCGACCAGCGGCTCGGGCCCGACGCGGTGCATCAGGGCATGCTGGCGGAAGT
CGACCCGCTGGAGTCGCCGCGGATCGACACCCTTCCCGAGGACGGCATCGTGCTGGTGCTCGACCAGATCACCGATCCGC
ACAATGTCGGCGCGATCATGCGCTCGGCCGCGGCGTTCGCCGTCAAGGCGATCGTCACCACCGCGCGGCACAGCCCGGAG
GCCACCGGCGTGCTCGCCAAATCCGCCTCGGGCGCGCTGGAGCTGGTGCCGCTGGTGCTGGTCACCAACCTCGCCCGCGC
GCTGACCGAACTCGAGGACCGCGGCTACCTCACCGTCGGGCTCGACAGCGAAGGCAGCGCCGACCTCGCCGCAGTCGAGC
TGCGGCAGCCGCTGGCGCTGGTGCTCGGCGCCGAGGGCAAGGGCCTGCGGCAGCTCACGCGCGAGACCTGCAACGTCGTG
GCGCGGCTCGACATGCCGGGCGCGATCAAGAGCCTCAACGTCTCCAACGCCGCGGCGCTGGCGCTGTATATCGGCGCCAG
CCGGCTCGGGCTGATGCGGTAG

Upstream 100 bases:

>100_bases
ATGCGCCCCCCTCAAGTCAACCAATCACATGACAATTTCTTGGGCCAATCGGCGGCATGACCAAATTGCCATGGGCGCGG
CCGCGTGGGACAACCGCGCG

Downstream 100 bases:

>100_bases
CCTGCAAGCGTCGACGCCCGCCCGCATCGCTGCGGACGGGCGCCCTGCCCTCGCCTGGACGGCGATCAGTAATAGCGGCG
CAGCGGCGCGTGGTGGCGAT

Product: RNA methyltransferase TrmH, group 3

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSPKERNPRFQRRDDRDRGRPQGSGGRHGARERDPDGPAILYGWHTVTAALANPARRIRKLLLTENAARKLADENINTRI
TPDIVRPGEIDQRLGPDAVHQGMLAEVDPLESPRIDTLPEDGIVLVLDQITDPHNVGAIMRSAAAFAVKAIVTTARHSPE
ATGVLAKSASGALELVPLVLVTNLARALTELEDRGYLTVGLDSEGSADLAAVELRQPLALVLGAEGKGLRQLTRETCNVV
ARLDMPGAIKSLNVSNAAALALYIGASRLGLMR

Sequences:

>Translated_273_residues
MSPKERNPRFQRRDDRDRGRPQGSGGRHGARERDPDGPAILYGWHTVTAALANPARRIRKLLLTENAARKLADENINTRI
TPDIVRPGEIDQRLGPDAVHQGMLAEVDPLESPRIDTLPEDGIVLVLDQITDPHNVGAIMRSAAAFAVKAIVTTARHSPE
ATGVLAKSASGALELVPLVLVTNLARALTELEDRGYLTVGLDSEGSADLAAVELRQPLALVLGAEGKGLRQLTRETCNVV
ARLDMPGAIKSLNVSNAAALALYIGASRLGLMR
>Mature_272_residues
SPKERNPRFQRRDDRDRGRPQGSGGRHGARERDPDGPAILYGWHTVTAALANPARRIRKLLLTENAARKLADENINTRIT
PDIVRPGEIDQRLGPDAVHQGMLAEVDPLESPRIDTLPEDGIVLVLDQITDPHNVGAIMRSAAAFAVKAIVTTARHSPEA
TGVLAKSASGALELVPLVLVTNLARALTELEDRGYLTVGLDSEGSADLAAVELRQPLALVLGAEGKGLRQLTRETCNVVA
RLDMPGAIKSLNVSNAAALALYIGASRLGLMR

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=197, Percent_Identity=35.0253807106599, Blast_Score=89, Evalue=5e-18,
Organism=Escherichia coli, GI1790623, Length=233, Percent_Identity=35.1931330472103, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1788935, Length=140, Percent_Identity=32.1428571428571, Blast_Score=70, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324775, Length=291, Percent_Identity=26.8041237113402, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 29278; Mature: 29147

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPKERNPRFQRRDDRDRGRPQGSGGRHGARERDPDGPAILYGWHTVTAALANPARRIRK
CCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHH
LLLTENAARKLADENINTRITPDIVRPGEIDQRLGPDAVHQGMLAEVDPLESPRIDTLPE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCHHHHHCCCCCCCCCCCCCCCCCCCC
DGIVLVLDQITDPHNVGAIMRSAAAFAVKAIVTTARHSPEATGVLAKSASGALELVPLVL
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEHHCCCCHHHHHHHHH
VTNLARALTELEDRGYLTVGLDSEGSADLAAVELRQPLALVLGAEGKGLRQLTRETCNVV
HHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHE
ARLDMPGAIKSLNVSNAAALALYIGASRLGLMR
EECCCCCCHHHCCCCCHHHHHEECCHHHHCCCC
>Mature Secondary Structure 
SPKERNPRFQRRDDRDRGRPQGSGGRHGARERDPDGPAILYGWHTVTAALANPARRIRK
CCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHH
LLLTENAARKLADENINTRITPDIVRPGEIDQRLGPDAVHQGMLAEVDPLESPRIDTLPE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCHHHHHCCCCCCCCCCCCCCCCCCCC
DGIVLVLDQITDPHNVGAIMRSAAAFAVKAIVTTARHSPEATGVLAKSASGALELVPLVL
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEHHCCCCHHHHHHHHH
VTNLARALTELEDRGYLTVGLDSEGSADLAAVELRQPLALVLGAEGKGLRQLTRETCNVV
HHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHE
ARLDMPGAIKSLNVSNAAALALYIGASRLGLMR
EECCCCCCHHHCCCCCHHHHHEECCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]