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The map label for this gene is ppnK [H]

Identifier: 86749316

GI number: 86749316

Start: 2489806

End: 2490585

Strand: Reverse

Name: ppnK [H]

Synonym: RPB_2196

Alternate gene names: 86749316

Gene position: 2490585-2489806 (Counterclockwise)

Preceding gene: 86749317

Following gene: 86749315

Centisome position: 46.71

GC content: 67.95

Gene sequence:

>780_bases
ATGACTCCCTCCCCGCGCCGCGACCGCATCGCCTTCGTCGCGAGCCAGAGCGCGGAAGCGCAAGCCGCACAGGCACAACT
CGTGTCGCTCTACGGCGATCACGACGCCGCCGAGGCCGACGTCGTGGTCGCGCTCGGCGGCGACGGGCTGATGCTGCAGA
CGCTGCATCAGCAGATGCGCAGCGGCCTGCCGATCTACGGCATGCACCGCGGCACGGTCGGCTTCCTGATGAACGAATAT
TCGATCCACGATCTGCGCGGACGGCTCGAGGCCGCGCGCGAGACCGTGATCCATCCGCTGCTGATGCGCGCCACCGACAT
CTACGGCGAGGTGCACATCCACCACGCCATCAACGAGGTCTCGCTGTTTCGGCAGAGCCATCAGGCGGCGCGGCTGCGCA
TCATCATCGACGAGCGCGAGCGCATGAGCGAGCTCGTCGCCGACGGCATTCTGGTCGCGACGCCGGCGGGCTCGACCGCG
TATAATCTGTCGGCGCAGGGCCCGATCCTGCCGATCAACGCGCAGTTACTTGCGCTGACGCCGATCTCGGCGTTCCGGCC
GCGGCGCTGGCGCGGCGCGCTGTTGCCGGACAACGCCTTCGTGGTGATCGAAGTGCTGGAAGTCGACAAGCGCCCGGTGG
CGGCGGTGGCCGATCACGACGAGGCGCGCGACGTGCGACGGGTCGAGGTGATTTCCGACAAGACCATCGCGCTGCGGATG
CTGTTCGACCCCGGCCACAGCCTCGAGGAACGCATCCTCAGCGAGCAGTTCGGCACCTGA

Upstream 100 bases:

>100_bases
GGCGGCTCGACAAAACCGGCAAAGGGCGTATAGACCAGCCCGGCGAAGCCCGCCTCCCGGCGGGTTTCGCGCCGTCCACG
CCCGCGCAGAGCTTCGCCGC

Downstream 100 bases:

>100_bases
CGGCGAGCGCCGCCGCGACGGGCTTTTTCAGTCGACAGATCAAGCACTTGCGCCTGACCTAGTGGGTCTTGCGGCAAACC
TGTGGCAGCGCCGCCGGCCG

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MTPSPRRDRIAFVASQSAEAQAAQAQLVSLYGDHDAAEADVVVALGGDGLMLQTLHQQMRSGLPIYGMHRGTVGFLMNEY
SIHDLRGRLEAARETVIHPLLMRATDIYGEVHIHHAINEVSLFRQSHQAARLRIIIDERERMSELVADGILVATPAGSTA
YNLSAQGPILPINAQLLALTPISAFRPRRWRGALLPDNAFVVIEVLEVDKRPVAAVADHDEARDVRRVEVISDKTIALRM
LFDPGHSLEERILSEQFGT

Sequences:

>Translated_259_residues
MTPSPRRDRIAFVASQSAEAQAAQAQLVSLYGDHDAAEADVVVALGGDGLMLQTLHQQMRSGLPIYGMHRGTVGFLMNEY
SIHDLRGRLEAARETVIHPLLMRATDIYGEVHIHHAINEVSLFRQSHQAARLRIIIDERERMSELVADGILVATPAGSTA
YNLSAQGPILPINAQLLALTPISAFRPRRWRGALLPDNAFVVIEVLEVDKRPVAAVADHDEARDVRRVEVISDKTIALRM
LFDPGHSLEERILSEQFGT
>Mature_258_residues
TPSPRRDRIAFVASQSAEAQAAQAQLVSLYGDHDAAEADVVVALGGDGLMLQTLHQQMRSGLPIYGMHRGTVGFLMNEYS
IHDLRGRLEAARETVIHPLLMRATDIYGEVHIHHAINEVSLFRQSHQAARLRIIIDERERMSELVADGILVATPAGSTAY
NLSAQGPILPINAQLLALTPISAFRPRRWRGALLPDNAFVVIEVLEVDKRPVAAVADHDEARDVRRVEVISDKTIALRML
FDPGHSLEERILSEQFGT

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788968, Length=153, Percent_Identity=32.0261437908497, Blast_Score=62, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322509, Length=216, Percent_Identity=27.7777777777778, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320794, Length=191, Percent_Identity=31.413612565445, Blast_Score=69, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6325068, Length=188, Percent_Identity=29.2553191489362, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 28616; Mature: 28485

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPSPRRDRIAFVASQSAEAQAAQAQLVSLYGDHDAAEADVVVALGGDGLMLQTLHQQMR
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHH
SGLPIYGMHRGTVGFLMNEYSIHDLRGRLEAARETVIHPLLMRATDIYGEVHIHHAINEV
CCCCEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEHHHHHHH
SLFRQSHQAARLRIIIDERERMSELVADGILVATPAGSTAYNLSAQGPILPINAQLLALT
HHHHHHHCCEEEEEEECCHHHHHHHHHCCEEEEECCCCCEEECCCCCCEEEECCEEEEEC
PISAFRPRRWRGALLPDNAFVVIEVLEVDKRPVAAVADHDEARDVRRVEVISDKTIALRM
CHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEECCCCHHHHHHEEEEECCCEEEEEE
LFDPGHSLEERILSEQFGT
EECCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TPSPRRDRIAFVASQSAEAQAAQAQLVSLYGDHDAAEADVVVALGGDGLMLQTLHQQMR
CCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHH
SGLPIYGMHRGTVGFLMNEYSIHDLRGRLEAARETVIHPLLMRATDIYGEVHIHHAINEV
CCCCEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEHHHHHHH
SLFRQSHQAARLRIIIDERERMSELVADGILVATPAGSTAYNLSAQGPILPINAQLLALT
HHHHHHHCCEEEEEEECCHHHHHHHHHCCEEEEECCCCCEEECCCCCCEEEECCEEEEEC
PISAFRPRRWRGALLPDNAFVVIEVLEVDKRPVAAVADHDEARDVRRVEVISDKTIALRM
CHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEECCCCHHHHHHEEEEECCCEEEEEE
LFDPGHSLEERILSEQFGT
EECCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA