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The map label for this gene is gatA [H]

Identifier: 86749246

GI number: 86749246

Start: 2417164

End: 2418579

Strand: Reverse

Name: gatA [H]

Synonym: RPB_2125

Alternate gene names: 86749246

Gene position: 2418579-2417164 (Counterclockwise)

Preceding gene: 86749247

Following gene: 86749245

Centisome position: 45.36

GC content: 68.79

Gene sequence:

>1416_bases
ATGTCTGACGGTTTGACGAGCAAGACCACCGCGCTGCACGACCTCAGCGCCACCGAACTGCTGGCCGGCTACTACGCCAA
ACAGTTCTCGCCGTCGGAGGTGATCGACGACGTCATCGCCCAGGCGCAGCGCTGGGAGCCGCATCTCAACGCGCTGTATG
CGTTCGATCCGGACGGCGCCCGCGCCGCGGCGAAGCAATCGACCGAGCGCTGGCACAAGGGCGAACCGCAGGGCGTGCTC
GACGGCGTGCCGGTGACGATCAAGGAGAACGTCGCGACCAAGGGCGTGCCGATTCCGCTCGGCGTGCCGGTCACCGCGCC
GACGCCGGCGTCGGTCGATGCGCCACCGGCGGCGCGGCTGCGCGAGGCCGGTGCGATCCTCTGGACCAAGACGACGATGC
CGGATTTCGGCATGCTGTCGTCCGGCCTGTCGAGCTTCCACGGCGTCACCCGCAATCCGTGGGACACCACGCAAAACACC
GGCGGCTCGTCCTCCGGCGCGGGCGCAGCGGGGGCTGCGGGCTACGGGCCGCTGCATCTCGGCACCGACATCGGCGGCTC
GGTGCGGCTGCCGGCGAACTGGTGCGGCCTGTTCGCGCTGAAGCCGTCGCTCGGCCGCGTGCCGATCGATCCGCCTTATG
TCGGCCGCTGCGCCGGGCCGATGACACGCACCGTCGACGATTCCGCGCTGATGATGAGCGTGCTGTCGCTGCCCGATGCG
CGCGACGGCATGAACCTGCCGCCGAACGATTTCGCCTGGCACGATCTGGCGACCGATCTGCGCGGCAAGACCATCGGCCT
CGTCACCGATCTCGGCTTCGGCATGAAGCTCGACGACGAGGTGCGCCGCGTCGTCACCGATGCCGCGCGAACCTTCGAGG
CGCAGGGCGCGATCGTGCGCGAGGTGTCGCTCGGCTTCACCCGCGAGATGATGGACGGGCTCGACAATTTCTGGCGGGCG
CGGATGTGGGACGACGTCTCCAAGCTCAGCGAGGCGCAGCGCGCGCAGGTGCTGCCCTACATCGTGCGCTGGGCCGAGCG
CGGCGCGACGCTCAGCGGCGTCGAGGTGATGCGCGGCTTCAACCAGACCATGGCGGTGCGGGCGATGGCGGCGAAACTGT
TCGCCGAGATCGACTATTTGATCTCGCCGGTGGCGCCGGTGGTGAGCTTCCCTGCCGAGCTGCCCTCGCCGCTCGACGAT
CCGGAGATTCCGTTCGACCACATCCTCTATACCGTGCCGTGGAATATGTCGGAGAATCCGGCGGCGTCGATCAATGGCGG
CTACAGTTCGAACGGCTTTCCGATCGGCGTGCAGATCGTCGGCCGACGCTTCGACGATCTCGGCGTGCTGCGGATGGCGA
AGGCGTTCGAGGGCCTGCGCGAGGCGCAGAAGCCGTGGCCGACCGTGCCGCGCTAG

Upstream 100 bases:

>100_bases
CCGGTGCACAATCCCGGCTACGTGCTCGACGACAAGATCCTGCCGATCGGCGCATCGATGTTCGCGCGGATCATCGAAAC
CCGTCTGCCGGTGGCCGCCA

Downstream 100 bases:

>100_bases
GCACGCGTTTCGCCGCACGCACGGTGTCATGCGCGGGCTCGACCCGCGCATCCATCTCCTTCATAAAGGATGGATGGCCG
GGTCAAGCCCGGCCATGACG

Product: amidase

Products: monocarboxylate; NH3

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MSDGLTSKTTALHDLSATELLAGYYAKQFSPSEVIDDVIAQAQRWEPHLNALYAFDPDGARAAAKQSTERWHKGEPQGVL
DGVPVTIKENVATKGVPIPLGVPVTAPTPASVDAPPAARLREAGAILWTKTTMPDFGMLSSGLSSFHGVTRNPWDTTQNT
GGSSSGAGAAGAAGYGPLHLGTDIGGSVRLPANWCGLFALKPSLGRVPIDPPYVGRCAGPMTRTVDDSALMMSVLSLPDA
RDGMNLPPNDFAWHDLATDLRGKTIGLVTDLGFGMKLDDEVRRVVTDAARTFEAQGAIVREVSLGFTREMMDGLDNFWRA
RMWDDVSKLSEAQRAQVLPYIVRWAERGATLSGVEVMRGFNQTMAVRAMAAKLFAEIDYLISPVAPVVSFPAELPSPLDD
PEIPFDHILYTVPWNMSENPAASINGGYSSNGFPIGVQIVGRRFDDLGVLRMAKAFEGLREAQKPWPTVPR

Sequences:

>Translated_471_residues
MSDGLTSKTTALHDLSATELLAGYYAKQFSPSEVIDDVIAQAQRWEPHLNALYAFDPDGARAAAKQSTERWHKGEPQGVL
DGVPVTIKENVATKGVPIPLGVPVTAPTPASVDAPPAARLREAGAILWTKTTMPDFGMLSSGLSSFHGVTRNPWDTTQNT
GGSSSGAGAAGAAGYGPLHLGTDIGGSVRLPANWCGLFALKPSLGRVPIDPPYVGRCAGPMTRTVDDSALMMSVLSLPDA
RDGMNLPPNDFAWHDLATDLRGKTIGLVTDLGFGMKLDDEVRRVVTDAARTFEAQGAIVREVSLGFTREMMDGLDNFWRA
RMWDDVSKLSEAQRAQVLPYIVRWAERGATLSGVEVMRGFNQTMAVRAMAAKLFAEIDYLISPVAPVVSFPAELPSPLDD
PEIPFDHILYTVPWNMSENPAASINGGYSSNGFPIGVQIVGRRFDDLGVLRMAKAFEGLREAQKPWPTVPR
>Mature_470_residues
SDGLTSKTTALHDLSATELLAGYYAKQFSPSEVIDDVIAQAQRWEPHLNALYAFDPDGARAAAKQSTERWHKGEPQGVLD
GVPVTIKENVATKGVPIPLGVPVTAPTPASVDAPPAARLREAGAILWTKTTMPDFGMLSSGLSSFHGVTRNPWDTTQNTG
GSSSGAGAAGAAGYGPLHLGTDIGGSVRLPANWCGLFALKPSLGRVPIDPPYVGRCAGPMTRTVDDSALMMSVLSLPDAR
DGMNLPPNDFAWHDLATDLRGKTIGLVTDLGFGMKLDDEVRRVVTDAARTFEAQGAIVREVSLGFTREMMDGLDNFWRAR
MWDDVSKLSEAQRAQVLPYIVRWAERGATLSGVEVMRGFNQTMAVRAMAAKLFAEIDYLISPVAPVVSFPAELPSPLDDP
EIPFDHILYTVPWNMSENPAASINGGYSSNGFPIGVQIVGRRFDDLGVLRMAKAFEGLREAQKPWPTVPR

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=517, Percent_Identity=28.6266924564797, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI195972892, Length=278, Percent_Identity=29.8561151079137, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI166795287, Length=282, Percent_Identity=29.4326241134752, Blast_Score=72, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17537465, Length=495, Percent_Identity=25.2525252525253, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17556264, Length=219, Percent_Identity=28.310502283105, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17538252, Length=161, Percent_Identity=35.4037267080745, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=130, Percent_Identity=37.6923076923077, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=271, Percent_Identity=28.0442804428044, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=271, Percent_Identity=28.0442804428044, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17538254, Length=529, Percent_Identity=24.7637051039698, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=395, Percent_Identity=24.0506329113924, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6320448, Length=166, Percent_Identity=36.144578313253, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323950, Length=176, Percent_Identity=31.25, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24648113, Length=508, Percent_Identity=26.5748031496063, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI21356731, Length=424, Percent_Identity=26.4150943396226, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI161078093, Length=490, Percent_Identity=25.1020408163265, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24644968, Length=490, Percent_Identity=23.0612244897959, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI45550774, Length=248, Percent_Identity=30.241935483871, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24648435, Length=248, Percent_Identity=30.241935483871, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24648437, Length=248, Percent_Identity=30.241935483871, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24652985, Length=499, Percent_Identity=25.8517034068136, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI19922090, Length=499, Percent_Identity=25.8517034068136, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24652981, Length=499, Percent_Identity=25.8517034068136, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24652983, Length=499, Percent_Identity=25.8517034068136, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24648441, Length=168, Percent_Identity=35.7142857142857, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24648439, Length=168, Percent_Identity=35.7142857142857, Blast_Score=90, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 3.5.1.4

Molecular weight: Translated: 50672; Mature: 50541

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDGLTSKTTALHDLSATELLAGYYAKQFSPSEVIDDVIAQAQRWEPHLNALYAFDPDGA
CCCCCCCCHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCH
RAAAKQSTERWHKGEPQGVLDGVPVTIKENVATKGVPIPLGVPVTAPTPASVDAPPAARL
HHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
REAGAILWTKTTMPDFGMLSSGLSSFHGVTRNPWDTTQNTGGSSSGAGAAGAAGYGPLHL
HHCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
GTDIGGSVRLPANWCGLFALKPSLGRVPIDPPYVGRCAGPMTRTVDDSALMMSVLSLPDA
CCCCCCCEECCHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
RDGMNLPPNDFAWHDLATDLRGKTIGLVTDLGFGMKLDDEVRRVVTDAARTFEAQGAIVR
CCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEE
EVSLGFTREMMDGLDNFWRARMWDDVSKLSEAQRAQVLPYIVRWAERGATLSGVEVMRGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCC
NQTMAVRAMAAKLFAEIDYLISPVAPVVSFPAELPSPLDDPEIPFDHILYTVPWNMSENP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCHHHEEEEECCCCCCCC
AASINGGYSSNGFPIGVQIVGRRFDDLGVLRMAKAFEGLREAQKPWPTVPR
CCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SDGLTSKTTALHDLSATELLAGYYAKQFSPSEVIDDVIAQAQRWEPHLNALYAFDPDGA
CCCCCCCHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCH
RAAAKQSTERWHKGEPQGVLDGVPVTIKENVATKGVPIPLGVPVTAPTPASVDAPPAARL
HHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
REAGAILWTKTTMPDFGMLSSGLSSFHGVTRNPWDTTQNTGGSSSGAGAAGAAGYGPLHL
HHCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
GTDIGGSVRLPANWCGLFALKPSLGRVPIDPPYVGRCAGPMTRTVDDSALMMSVLSLPDA
CCCCCCCEECCHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
RDGMNLPPNDFAWHDLATDLRGKTIGLVTDLGFGMKLDDEVRRVVTDAARTFEAQGAIVR
CCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEE
EVSLGFTREMMDGLDNFWRARMWDDVSKLSEAQRAQVLPYIVRWAERGATLSGVEVMRGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCC
NQTMAVRAMAAKLFAEIDYLISPVAPVVSFPAELPSPLDDPEIPFDHILYTVPWNMSENP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCHHHEEEEECCCCCCCC
AASINGGYSSNGFPIGVQIVGRRFDDLGVLRMAKAFEGLREAQKPWPTVPR
CCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: monocarboxylic acid amide; H2O

Specific reaction: A monocarboxylic acid amide + H2O = a monocarboxylate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA