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The map label for this gene is sseA [H]

Identifier: 86749038

GI number: 86749038

Start: 2188485

End: 2189342

Strand: Direct

Name: sseA [H]

Synonym: RPB_1915

Alternate gene names: 86749038

Gene position: 2188485-2189342 (Clockwise)

Preceding gene: 86749037

Following gene: 86749040

Centisome position: 41.05

GC content: 67.83

Gene sequence:

>858_bases
ATGACTGCCATTGAAGATCCGTTGGTCTCCACCGATTGGCTTGCCGCGCATCTCGGCGATCCCCAGGTCAAGGTGCTCGA
CGCCTCGTTCAAGATGCCGGGCGTGCTGCCGCGGCCCGGCGACGATTATCTCGCGGCGCACATTCCCGGCGCGGTGTATT
TCGACGTCGAGGAAGTCTGCGACCGCGCCGATGCGCGGCCGCATATGTATCCGGACGCGGCGCAGTTCGCGCGCGACGTC
GCGGCGATGGGCGTCTCGTCTGGCGATACCGTAGTCGTCTACGATGCCGGCGGCTGGGTCGCAGCGCCCCGGGCGTGGTG
GATGTTCCTGTCTTTCGGCCACGCCGACGTTCGCATCCTCGAGGGCGGCCTGAAGAAGTGGCAGGCGGAAGACCGGCCGA
CCGAGTCAGGAAAGCCGTCGCCGAAGCCGGGCGTCTTCACCGCAACGCTCGATCCGACCTATCTACGCAGCAAGGAGCAG
TTGGTCGCCAATTTCGACAGCGGCGCCGAGCAGGTGATCGACGCTCGTGCCGCCGATCGGTTCGAGGGCCGCGTGCCGGA
GCCGCGCCCCGGCCTGCGCGCGGGCCATATCCCGGGCAGCCGCAATCTGCCTTACAACGAACTGTTCGACGCCGCGACCG
GGCTGATGAAGTCACGCGATGAGTTGCGGAAGGCTTTCGTCCGGGCCGGCGTCGATCTCGACGGCCCGATCGTCACCAGT
TGCGGCTCGGGCGTGTCGGCGCTGGTGTTGACGCTGGCATTGTACCGGCTCGGCGTCCGCGGCACCGCGCTGTACGACGG
CTCTTGGTCGGAGTGGGGGATGCCGGATGGTCCGCCGATCGCGACGGGGCCGGCTTAG

Upstream 100 bases:

>100_bases
GCGCGGACTTGACCGGCGTCGCTGTTTTCTCAATGACGATGCCGCGCCGCAGAGTTTCCGGCGATGTTGTGTTTCATTCG
CCTGCGGCGGAGACTTCCCG

Downstream 100 bases:

>100_bases
GCCTAGCCCGCCTCAGGTCGGCGGCAGGCCGAACGGCGATTCCTCGGAAGACGGCCGGGCGACGACCGGACGCCGGCGCC
GCTCCTCGGCCTGACGGCGC

Product: 3-mercaptopyruvate sulfurtransferase

Products: NA

Alternate protein names: MST; Rhodanese-like protein [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTAIEDPLVSTDWLAAHLGDPQVKVLDASFKMPGVLPRPGDDYLAAHIPGAVYFDVEEVCDRADARPHMYPDAAQFARDV
AAMGVSSGDTVVVYDAGGWVAAPRAWWMFLSFGHADVRILEGGLKKWQAEDRPTESGKPSPKPGVFTATLDPTYLRSKEQ
LVANFDSGAEQVIDARAADRFEGRVPEPRPGLRAGHIPGSRNLPYNELFDAATGLMKSRDELRKAFVRAGVDLDGPIVTS
CGSGVSALVLTLALYRLGVRGTALYDGSWSEWGMPDGPPIATGPA

Sequences:

>Translated_285_residues
MTAIEDPLVSTDWLAAHLGDPQVKVLDASFKMPGVLPRPGDDYLAAHIPGAVYFDVEEVCDRADARPHMYPDAAQFARDV
AAMGVSSGDTVVVYDAGGWVAAPRAWWMFLSFGHADVRILEGGLKKWQAEDRPTESGKPSPKPGVFTATLDPTYLRSKEQ
LVANFDSGAEQVIDARAADRFEGRVPEPRPGLRAGHIPGSRNLPYNELFDAATGLMKSRDELRKAFVRAGVDLDGPIVTS
CGSGVSALVLTLALYRLGVRGTALYDGSWSEWGMPDGPPIATGPA
>Mature_284_residues
TAIEDPLVSTDWLAAHLGDPQVKVLDASFKMPGVLPRPGDDYLAAHIPGAVYFDVEEVCDRADARPHMYPDAAQFARDVA
AMGVSSGDTVVVYDAGGWVAAPRAWWMFLSFGHADVRILEGGLKKWQAEDRPTESGKPSPKPGVFTATLDPTYLRSKEQL
VANFDSGAEQVIDARAADRFEGRVPEPRPGLRAGHIPGSRNLPYNELFDAATGLMKSRDELRKAFVRAGVDLDGPIVTSC
GSGVSALVLTLALYRLGVRGTALYDGSWSEWGMPDGPPIATGPA

Specific function: Transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI194473668, Length=273, Percent_Identity=42.1245421245421, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI61835204, Length=273, Percent_Identity=42.1245421245421, Blast_Score=222, Evalue=2e-58,
Organism=Homo sapiens, GI194473681, Length=273, Percent_Identity=42.1245421245421, Blast_Score=222, Evalue=2e-58,
Organism=Homo sapiens, GI17402865, Length=272, Percent_Identity=43.0147058823529, Blast_Score=205, Evalue=3e-53,
Organism=Escherichia coli, GI87082121, Length=268, Percent_Identity=45.5223880597015, Blast_Score=228, Evalue=4e-61,
Organism=Escherichia coli, GI87081967, Length=273, Percent_Identity=26.3736263736264, Blast_Score=74, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17561888, Length=292, Percent_Identity=28.4246575342466, Blast_Score=104, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI115534702, Length=212, Percent_Identity=30.188679245283, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17543836, Length=291, Percent_Identity=27.4914089347079, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17543838, Length=291, Percent_Identity=27.4914089347079, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71997283, Length=294, Percent_Identity=25.8503401360544, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17559150, Length=278, Percent_Identity=25.8992805755396, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324825, Length=271, Percent_Identity=32.1033210332103, Blast_Score=138, Evalue=1e-33,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.2 [H]

Molecular weight: Translated: 30607; Mature: 30476

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00380 RHODANESE_1 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIEDPLVSTDWLAAHLGDPQVKVLDASFKMPGVLPRPGDDYLAAHIPGAVYFDVEEVC
CCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCEEEECCCEEEECHHHHH
DRADARPHMYPDAAQFARDVAAMGVSSGDTVVVYDAGGWVAAPRAWWMFLSFGHADVRIL
HHCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCEECCHHEEHHHHCCCCCEEEH
EGGLKKWQAEDRPTESGKPSPKPGVFTATLDPTYLRSKEQLVANFDSGAEQVIDARAADR
HHHHHHHCCCCCCCCCCCCCCCCCEEEEECCHHHHHCHHHHHHHCCCHHHHHHHHHHHHH
FEGRVPEPRPGLRAGHIPGSRNLPYNELFDAATGLMKSRDELRKAFVRAGVDLDGPIVTS
HCCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEC
CGSGVSALVLTLALYRLGVRGTALYDGSWSEWGMPDGPPIATGPA
CCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TAIEDPLVSTDWLAAHLGDPQVKVLDASFKMPGVLPRPGDDYLAAHIPGAVYFDVEEVC
CCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCEEEECCCEEEECHHHHH
DRADARPHMYPDAAQFARDVAAMGVSSGDTVVVYDAGGWVAAPRAWWMFLSFGHADVRIL
HHCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCEECCHHEEHHHHCCCCCEEEH
EGGLKKWQAEDRPTESGKPSPKPGVFTATLDPTYLRSKEQLVANFDSGAEQVIDARAADR
HHHHHHHCCCCCCCCCCCCCCCCCEEEEECCHHHHHCHHHHHHHCCCHHHHHHHHHHHHH
FEGRVPEPRPGLRAGHIPGSRNLPYNELFDAATGLMKSRDELRKAFVRAGVDLDGPIVTS
HCCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEC
CGSGVSALVLTLALYRLGVRGTALYDGSWSEWGMPDGPPIATGPA
CCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]