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The map label for this gene is bphC [H]

Identifier: 86748828

GI number: 86748828

Start: 1940200

End: 1941177

Strand: Reverse

Name: bphC [H]

Synonym: RPB_1705

Alternate gene names: 86748828

Gene position: 1941177-1940200 (Counterclockwise)

Preceding gene: 86748829

Following gene: 86748827

Centisome position: 36.41

GC content: 65.34

Gene sequence:

>978_bases
ATGCCTGTGCCGCAGCACGTATTCGACCCGCCGTTCAACATCATCCGTTGCAGTCACGTCGTGCTCGACGTCACCGACCT
TTCCGCCAGCGTCGATTTCTATGCCGACATCATCGGCCTGCATGTCGAGGATCGCGACGACGCCACCGCCTATCTACGCG
GCAGCGAGGAGCATCAGCATCACTCGGTGGTACTGCGCCAGGCGGACAAGCCGGCCGCGGCGCGGCTCGGCTTTCGCGTC
GGCACCGAAGCCGATCTCGACAAGGCCGGCAGCTTCTTCGCCGAGAATGGGCTGATCTACAGCTTCGTCGATCGCCCATT
CCAGGGCCGCACGCTGCACGTCACCGACCCGTTCGGCTTCCGGCTCGAGTTCTGCGCCAGCATGGAGAAGCGGCCGCATC
TTCTGCGCCGCTACGAATTGTACAAAGGCTGTCACCCGCAGCGGCTCGACCATTTCAACGTCTTCGCGGCCGAGACTCAG
GAGACCATCGACTTCTACGCCCGGCTCGGCTTTCGCCTCACCGAATACGCCGAAGAGGACGGCGACAACGGCCGCATCGC
CGCGGCCTGGATGCATCGCAAAGGCAACGTCCACGACTTCGCCGTCACCAACGGCCGCGGCCCGCGGCTGCATCATTTCG
CCTATTGGGTGCCCGGTCCGCTCAACATCATCCATCTCTGCGACGTGATGGCGTCGCGGGGGCTCGGCCTCGAGCGCGGC
CCGGGCCGCCACGGCATCTCGAACGCCTTCTTTCTCTACGTCCGCGATCCCGACGGCCATCGTATCGAGCTGTATTGCAG
CGACTATCAGACCATGGACCACGACCACGCCCCGCTGCGCTGGTCGCTACGCGACCCGCGCCGGCAGACGCTGTGGGGCG
CGCCGGCGCCGCGCTCCTGGTTCGAGCAGGGCTCGGATTTCCTCGGCGAGACGGTTCGCGAGCCGGCATTCGTGGCCGAT
GTGATGATTGCGGATTGA

Upstream 100 bases:

>100_bases
GCACCGCTCGTGTCCATCATCTCGGGATTCCGGGTTCGGGCCGCTCGCGCCCCGGAATGACGTTGCTGAATTTACACCAA
GATCACTGGGGACCTCGCCA

Downstream 100 bases:

>100_bases
CCGCCGTCATCGCCCGGCCTGTCCGGGCGAGCCAGTATGGCAGAGCGCTGGTTCGAACCATGACCGCTCTGGAATACTGG
GTCACCCGCTGTCGCGGGTG

Product: 3,4-dihydroxyphenylacetate 2,3-dioxygenase HpaD

Products: NA

Alternate protein names: Mn(II)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase; Mn(II)-dependent 23OHBP oxygenase [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MPVPQHVFDPPFNIIRCSHVVLDVTDLSASVDFYADIIGLHVEDRDDATAYLRGSEEHQHHSVVLRQADKPAAARLGFRV
GTEADLDKAGSFFAENGLIYSFVDRPFQGRTLHVTDPFGFRLEFCASMEKRPHLLRRYELYKGCHPQRLDHFNVFAAETQ
ETIDFYARLGFRLTEYAEEDGDNGRIAAAWMHRKGNVHDFAVTNGRGPRLHHFAYWVPGPLNIIHLCDVMASRGLGLERG
PGRHGISNAFFLYVRDPDGHRIELYCSDYQTMDHDHAPLRWSLRDPRRQTLWGAPAPRSWFEQGSDFLGETVREPAFVAD
VMIAD

Sequences:

>Translated_325_residues
MPVPQHVFDPPFNIIRCSHVVLDVTDLSASVDFYADIIGLHVEDRDDATAYLRGSEEHQHHSVVLRQADKPAAARLGFRV
GTEADLDKAGSFFAENGLIYSFVDRPFQGRTLHVTDPFGFRLEFCASMEKRPHLLRRYELYKGCHPQRLDHFNVFAAETQ
ETIDFYARLGFRLTEYAEEDGDNGRIAAAWMHRKGNVHDFAVTNGRGPRLHHFAYWVPGPLNIIHLCDVMASRGLGLERG
PGRHGISNAFFLYVRDPDGHRIELYCSDYQTMDHDHAPLRWSLRDPRRQTLWGAPAPRSWFEQGSDFLGETVREPAFVAD
VMIAD
>Mature_324_residues
PVPQHVFDPPFNIIRCSHVVLDVTDLSASVDFYADIIGLHVEDRDDATAYLRGSEEHQHHSVVLRQADKPAAARLGFRVG
TEADLDKAGSFFAENGLIYSFVDRPFQGRTLHVTDPFGFRLEFCASMEKRPHLLRRYELYKGCHPQRLDHFNVFAAETQE
TIDFYARLGFRLTEYAEEDGDNGRIAAAWMHRKGNVHDFAVTNGRGPRLHHFAYWVPGPLNIIHLCDVMASRGLGLERGP
GRHGISNAFFLYVRDPDGHRIELYCSDYQTMDHDHAPLRWSLRDPRRQTLWGAPAPRSWFEQGSDFLGETVREPAFVADV
MIAD

Specific function: Catalyzes the meta-cleavage of the hydroxylated biphenyl ring. The enzyme can oxidize a wide range of substrates, and the substrate preference order is 2,3-dihydroxybiphenyl > 3- methylcatechol > catechol > 4-methylcatechol > 4-chlorocatechol [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the extradiol ring-cleavage dioxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =1.13.11.39 [H]

Molecular weight: Translated: 37090; Mature: 36959

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00082 EXTRADIOL_DIOXYGENAS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVPQHVFDPPFNIIRCSHVVLDVTDLSASVDFYADIIGLHVEDRDDATAYLRGSEEHQH
CCCCCCCCCCCHHHEEEEEEEEEEECCCCCHHHEEEEEEEEEECCCCCEEEECCCCCCCH
HSVVLRQADKPAAARLGFRVGTEADLDKAGSFFAENGLIYSFVDRPFQGRTLHVTDPFGF
HHHEEECCCCCHHHHHCEECCCCCCHHHHCCEECCCCEEEEECCCCCCCCEEEEECCCCC
RLEFCASMEKRPHLLRRYELYKGCHPQRLDHFNVFAAETQETIDFYARLGFRLTEYAEED
HHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHCC
GDNGRIAAAWMHRKGNVHDFAVTNGRGPRLHHFAYWVPGPLNIIHLCDVMASRGLGLERG
CCCCEEEEEEEECCCCEEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCC
PGRHGISNAFFLYVRDPDGHRIELYCSDYQTMDHDHAPLRWSLRDPRRQTLWGAPAPRSW
CCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCEEECCCCCHHHHCCCCCCCHHH
FEQGSDFLGETVREPAFVADVMIAD
HHCCHHHHHHHHCCCCEEEEEEECC
>Mature Secondary Structure 
PVPQHVFDPPFNIIRCSHVVLDVTDLSASVDFYADIIGLHVEDRDDATAYLRGSEEHQH
CCCCCCCCCCHHHEEEEEEEEEEECCCCCHHHEEEEEEEEEECCCCCEEEECCCCCCCH
HSVVLRQADKPAAARLGFRVGTEADLDKAGSFFAENGLIYSFVDRPFQGRTLHVTDPFGF
HHHEEECCCCCHHHHHCEECCCCCCHHHHCCEECCCCEEEEECCCCCCCCEEEEECCCCC
RLEFCASMEKRPHLLRRYELYKGCHPQRLDHFNVFAAETQETIDFYARLGFRLTEYAEED
HHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHCC
GDNGRIAAAWMHRKGNVHDFAVTNGRGPRLHHFAYWVPGPLNIIHLCDVMASRGLGLERG
CCCCEEEEEEEECCCCEEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCC
PGRHGISNAFFLYVRDPDGHRIELYCSDYQTMDHDHAPLRWSLRDPRRQTLWGAPAPRSW
CCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCEEECCCCCHHHHCCCCCCCHHH
FEQGSDFLGETVREPAFVADVMIAD
HHCCHHHHHHHHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12672826 [H]