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The map label for this gene is ilvB [H]

Identifier: 86748826

GI number: 86748826

Start: 1937547

End: 1939223

Strand: Reverse

Name: ilvB [H]

Synonym: RPB_1703

Alternate gene names: 86748826

Gene position: 1939223-1937547 (Counterclockwise)

Preceding gene: 86748827

Following gene: 86748822

Centisome position: 36.37

GC content: 68.1

Gene sequence:

>1677_bases
ATGGCGTCGTCGACGAAGCCGACTAGCTCCAATCCAACAACGAGACCCAAAATGAGCATCATGACCGGCGGCGAAGCGAT
CGTGCAGACCCTCGTCGCGCACGGCGTCGACACCGTGTTCGGCCTGCCCGGCGCGCAGATCTACGGGTTGTTCGACGGCT
TCGCAAAGGCGCAACTCCGCGTCGTCGGTGCGCGCCATGAGCAGGCCTGCGGCTACATGGCGTTCGGCTATGCCCGCGCC
TCGGGCCGCCCCGGCGTGTTCAGCGTCGTGCCCGGCCCCGGCGTGCTGAATGCCGGCGCGGCGCTGCTCACCGCCTTCGG
CTGCAACGAGCCGGTGCTGTGCCTCACCGGTCAGGTGCCGAGCGCCTATCTCGGCAAGGGCCGTGGTCACCTGCATGAGA
TGCCGGATCAGCTCGCGACGTTGCGCAGCTTCATCAAATGGGCCGAGCGCATGGAGTATCCCGGCAACGCGCCGGCGCTG
GTCGCGCGCGCGTTCCAGGAGATGATGAGCGGCCGGCGCGGCCCGGTGGCGCTGGAAATGCCCTGGGAGGTGTTCACCCA
GCGCGCCGAGACCGCAGCGGCGATCAAGCTCGATCCGGTCGTACCGCCGCAGCCCGACCCGGACCGCGTCGCCGCCGCCG
CGAAGCTGATCGCCGCGAGCAAGACGCCGATGATCTTCGTCGGCTCCGGTGCGCTCGACGCTGGCGACGAGATCCTCGAA
CTGGCCGAAGCGATCGACGCGCCGGTCGTCGCATTTCGCAGCGGCCGCGGCATTGTCAGTAACCGGCACGACCTCGGCCT
GACCTTCGCCGCCGCCTATCGGCTGTGGCCGCAGACCGATCTGATCATCGGCATCGGCTCGCGGATGGAACTGCCGACGA
CGTTCCGCTGGCCGTTCCGGCCGGACGGCCAGAAGTCGGTGCGGATCGACATCGATCCCGCCGAGATGCGCCGCTTTTCG
CCGGACGCTTCGATCGTCGCCGATGCCAAGGCCGGCACTCGTGCGCTGGTCGACGCGGTGAGCAAGCGTGGCTACAACAA
GACCCAAGGGCGGCGCGCGACCATTCGCGAGGCGACCGCGCTCACGCTGGAAGCGATCCAGTCGGTGCAGCCGCAGATGG
CCTATTTGAAGATCCTGCGCGAGGTGCTGCCGGACGACGCCATCGTCACCGACGAGCTGTCGCAGGTTGGATTCGCCTCG
TGGTACGGCTTCCCGATCTACCAGCCCCGCACCTTTCTCACATCGGGCTATCAGGGCACGCTCGGCTCCGGCTTCCCGAC
CGCGCTCGGCGCCAAGGTCGCCTGCCCCGACAAGCCGGTCGTCGCCATCACCGGCGACGGCGGTTTCATGTTCGCTGTGC
AGGAGCTCGCCACCGCGGTGCAGTTCAACATCGGCGTGGTGACGCTGGTGTTCGACAATTCGGCCTATGGCAACGTCCGG
CGCGACCAGGTCACCCAGTTCGAAGGCCGCGTGGTGGCGTCCGATCTGGTCAATCCGGATTTCGTCAAGCTCGCGGAATC
CTTCGGCGTCGCGGCGTCGCGGGTCGGCTCGCCCGATCACTTCCGCGCCGCGCTGGAGAAGGCGCTGGCGCATGGCGGGC
CGTATCTGATCGCGATCGACGTTCCGCGCGACAGCGAAGCCAGCCCCTGGCCGTTCATCCATCCGGCGAAGCCGTGA

Upstream 100 bases:

>100_bases
GGCACGCCGGCCGGCGCCGGCGCACGCTCCGACCCGCCGGTGTACTTGAAGCCCGGCGATATCATCGAGGTCGAGGCCGA
CGGCATCGGCGTGCTCCGCA

Downstream 100 bases:

>100_bases
GCACGGCCCATCATGGTGTGAGGCCCCGGGAGAATTCCGAGGTCGCCGCTGCGCGGCGCTCCGGAATGACGAGCTCTCCA
ATCAGCTCGTCGCTGTGACT

Product: hypothetical protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 558; Mature: 557

Protein sequence:

>558_residues
MASSTKPTSSNPTTRPKMSIMTGGEAIVQTLVAHGVDTVFGLPGAQIYGLFDGFAKAQLRVVGARHEQACGYMAFGYARA
SGRPGVFSVVPGPGVLNAGAALLTAFGCNEPVLCLTGQVPSAYLGKGRGHLHEMPDQLATLRSFIKWAERMEYPGNAPAL
VARAFQEMMSGRRGPVALEMPWEVFTQRAETAAAIKLDPVVPPQPDPDRVAAAAKLIAASKTPMIFVGSGALDAGDEILE
LAEAIDAPVVAFRSGRGIVSNRHDLGLTFAAAYRLWPQTDLIIGIGSRMELPTTFRWPFRPDGQKSVRIDIDPAEMRRFS
PDASIVADAKAGTRALVDAVSKRGYNKTQGRRATIREATALTLEAIQSVQPQMAYLKILREVLPDDAIVTDELSQVGFAS
WYGFPIYQPRTFLTSGYQGTLGSGFPTALGAKVACPDKPVVAITGDGGFMFAVQELATAVQFNIGVVTLVFDNSAYGNVR
RDQVTQFEGRVVASDLVNPDFVKLAESFGVAASRVGSPDHFRAALEKALAHGGPYLIAIDVPRDSEASPWPFIHPAKP

Sequences:

>Translated_558_residues
MASSTKPTSSNPTTRPKMSIMTGGEAIVQTLVAHGVDTVFGLPGAQIYGLFDGFAKAQLRVVGARHEQACGYMAFGYARA
SGRPGVFSVVPGPGVLNAGAALLTAFGCNEPVLCLTGQVPSAYLGKGRGHLHEMPDQLATLRSFIKWAERMEYPGNAPAL
VARAFQEMMSGRRGPVALEMPWEVFTQRAETAAAIKLDPVVPPQPDPDRVAAAAKLIAASKTPMIFVGSGALDAGDEILE
LAEAIDAPVVAFRSGRGIVSNRHDLGLTFAAAYRLWPQTDLIIGIGSRMELPTTFRWPFRPDGQKSVRIDIDPAEMRRFS
PDASIVADAKAGTRALVDAVSKRGYNKTQGRRATIREATALTLEAIQSVQPQMAYLKILREVLPDDAIVTDELSQVGFAS
WYGFPIYQPRTFLTSGYQGTLGSGFPTALGAKVACPDKPVVAITGDGGFMFAVQELATAVQFNIGVVTLVFDNSAYGNVR
RDQVTQFEGRVVASDLVNPDFVKLAESFGVAASRVGSPDHFRAALEKALAHGGPYLIAIDVPRDSEASPWPFIHPAKP
>Mature_557_residues
ASSTKPTSSNPTTRPKMSIMTGGEAIVQTLVAHGVDTVFGLPGAQIYGLFDGFAKAQLRVVGARHEQACGYMAFGYARAS
GRPGVFSVVPGPGVLNAGAALLTAFGCNEPVLCLTGQVPSAYLGKGRGHLHEMPDQLATLRSFIKWAERMEYPGNAPALV
ARAFQEMMSGRRGPVALEMPWEVFTQRAETAAAIKLDPVVPPQPDPDRVAAAAKLIAASKTPMIFVGSGALDAGDEILEL
AEAIDAPVVAFRSGRGIVSNRHDLGLTFAAAYRLWPQTDLIIGIGSRMELPTTFRWPFRPDGQKSVRIDIDPAEMRRFSP
DASIVADAKAGTRALVDAVSKRGYNKTQGRRATIREATALTLEAIQSVQPQMAYLKILREVLPDDAIVTDELSQVGFASW
YGFPIYQPRTFLTSGYQGTLGSGFPTALGAKVACPDKPVVAITGDGGFMFAVQELATAVQFNIGVVTLVFDNSAYGNVRR
DQVTQFEGRVVASDLVNPDFVKLAESFGVAASRVGSPDHFRAALEKALAHGGPYLIAIDVPRDSEASPWPFIHPAKP

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=553, Percent_Identity=24.4122965641953, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI21361361, Length=549, Percent_Identity=27.1402550091075, Blast_Score=137, Evalue=3e-32,
Organism=Escherichia coli, GI87081685, Length=565, Percent_Identity=29.0265486725664, Blast_Score=222, Evalue=4e-59,
Organism=Escherichia coli, GI1790104, Length=563, Percent_Identity=27.886323268206, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI1786717, Length=569, Percent_Identity=30.5799648506151, Blast_Score=195, Evalue=7e-51,
Organism=Escherichia coli, GI1787096, Length=546, Percent_Identity=26.9230769230769, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1788716, Length=546, Percent_Identity=25.2747252747253, Blast_Score=139, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17542570, Length=580, Percent_Identity=26.551724137931, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17531299, Length=563, Percent_Identity=25.9325044404973, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17531301, Length=563, Percent_Identity=25.9325044404973, Blast_Score=124, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6323755, Length=567, Percent_Identity=29.4532627865961, Blast_Score=199, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6320816, Length=485, Percent_Identity=26.3917525773196, Blast_Score=138, Evalue=3e-33,
Organism=Drosophila melanogaster, GI19922626, Length=571, Percent_Identity=26.7950963222417, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59615; Mature: 59484

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSTKPTSSNPTTRPKMSIMTGGEAIVQTLVAHGVDTVFGLPGAQIYGLFDGFAKAQLR
CCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHEEEE
VVGARHEQACGYMAFGYARASGRPGVFSVVPGPGVLNAGAALLTAFGCNEPVLCLTGQVP
EEECCCHHHCCEEEEEEEECCCCCCEEEECCCCCCHHCCHHHHHHHCCCCCEEEEECCCC
SAYLGKGRGHLHEMPDQLATLRSFIKWAERMEYPGNAPALVARAFQEMMSGRRGPVALEM
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEC
PWEVFTQRAETAAAIKLDPVVPPQPDPDRVAAAAKLIAASKTPMIFVGSGALDAGDEILE
CHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHH
LAEAIDAPVVAFRSGRGIVSNRHDLGLTFAAAYRLWPQTDLIIGIGSRMELPTTFRWPFR
HHHHHCCCEEEEECCCCCCCCCCCCCEEEEHHHHHCCCCCEEEECCCCCCCCCEEECCCC
PDGQKSVRIDIDPAEMRRFSPDASIVADAKAGTRALVDAVSKRGYNKTQGRRATIREATA
CCCCCEEEEECCHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHH
LTLEAIQSVQPQMAYLKILREVLPDDAIVTDELSQVGFASWYGFPIYQPRTFLTSGYQGT
HHHHHHHHCCHHHHHHHHHHHHCCCCCEEHHHHHHCCHHHHCCCCCCCCHHHHCCCCCCC
LGSGFPTALGAKVACPDKPVVAITGDGGFMFAVQELATAVQFNIGVVTLVFDNSAYGNVR
CCCCCCHHHCCEEECCCCCEEEEECCCCHHHHHHHHHHHHEECCEEEEEEECCCCCCCCC
RDQVTQFEGRVVASDLVNPDFVKLAESFGVAASRVGSPDHFRAALEKALAHGGPYLIAID
HHHHHHHCCEEEEHHCCCHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEE
VPRDSEASPWPFIHPAKP
CCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ASSTKPTSSNPTTRPKMSIMTGGEAIVQTLVAHGVDTVFGLPGAQIYGLFDGFAKAQLR
CCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHEEEE
VVGARHEQACGYMAFGYARASGRPGVFSVVPGPGVLNAGAALLTAFGCNEPVLCLTGQVP
EEECCCHHHCCEEEEEEEECCCCCCEEEECCCCCCHHCCHHHHHHHCCCCCEEEEECCCC
SAYLGKGRGHLHEMPDQLATLRSFIKWAERMEYPGNAPALVARAFQEMMSGRRGPVALEM
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEC
PWEVFTQRAETAAAIKLDPVVPPQPDPDRVAAAAKLIAASKTPMIFVGSGALDAGDEILE
CHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHH
LAEAIDAPVVAFRSGRGIVSNRHDLGLTFAAAYRLWPQTDLIIGIGSRMELPTTFRWPFR
HHHHHCCCEEEEECCCCCCCCCCCCCEEEEHHHHHCCCCCEEEECCCCCCCCCEEECCCC
PDGQKSVRIDIDPAEMRRFSPDASIVADAKAGTRALVDAVSKRGYNKTQGRRATIREATA
CCCCCEEEEECCHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHH
LTLEAIQSVQPQMAYLKILREVLPDDAIVTDELSQVGFASWYGFPIYQPRTFLTSGYQGT
HHHHHHHHCCHHHHHHHHHHHHCCCCCEEHHHHHHCCHHHHCCCCCCCCHHHHCCCCCCC
LGSGFPTALGAKVACPDKPVVAITGDGGFMFAVQELATAVQFNIGVVTLVFDNSAYGNVR
CCCCCCHHHCCEEECCCCCEEEEECCCCHHHHHHHHHHHHEECCEEEEEEECCCCCCCCC
RDQVTQFEGRVVASDLVNPDFVKLAESFGVAASRVGSPDHFRAALEKALAHGGPYLIAID
HHHHHHHCCEEEEHHCCCHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEE
VPRDSEASPWPFIHPAKP
CCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]