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The map label for this gene is lcfB [H]

Identifier: 86748804

GI number: 86748804

Start: 1913283

End: 1914827

Strand: Reverse

Name: lcfB [H]

Synonym: RPB_1681

Alternate gene names: 86748804

Gene position: 1914827-1913283 (Counterclockwise)

Preceding gene: 86748805

Following gene: 86748803

Centisome position: 35.91

GC content: 64.79

Gene sequence:

>1545_bases
ATGACCCATCCGTCGATTCACGCCCGCACCCAGCCCGACAAGATCGCCTATCAGATGGCCTCGACCGGCAAGGCGATTAC
GTATCGCGAGCTCGACGAGCTCTCGAACCAGGGCGCGCAACTGTTCCGCACGCTCGGCCTGAAGGCCGGCGATCACGTCG
CGCTGCTGATGGAGAATCGCCTCGCCTTCATGGAGATCTGCTGGGCGGCGCAGCGCGCGGGCCTCTACTACACGGCGATC
AGCCGCTACCTGAAGCAGGACGAGATCGCCTATATCGTGCAGGATTGCGGCGCCCGTGTCGTGATTACCTCGCCGCAGGG
CGCGGACGCGATCGCGCCGCTGGTGAGCACCCTGCCCGGCGTCGCCTTCTTCATGGTCGATGAACCCGCTCCGGGTTTCC
GCTCCTGGGACAAGGAGGCCGGCGCGCAGCCGACGACGCCGATCGCCGACGAAGTCGCCGGCTACGACATGCTGTATTCG
TCCGGCACCACCGGCCGCCCCAAGGGCATCAAGCGCGAATTCGAAGGCAACGCCATCGACGTGCCGAGCCCGTTTCTGCG
TCTGCTGTGCGAGCGGATGTGCGGCATGAACCAGGACAGCATCTATCTGTCGCCGGCGCCGCTGTATCACGCCGCTCCGC
TGCGCTTCAACATGATGGCGACCACGCTCGGCGGCACCTCGGTGATCATGGAGCATTTCGACGCCGAGCAGTTCCTGGCG
CTGGTCGAGAAGTACAAGGTGACCCAGTCGCAACTCGTTCCGACGATGTTCGTGCGGATGCTGAAGCTGCCGGACGAGGT
CCGCACCCGCTACGACGTCTCGTCGTTGAAGGGCGCGATCCATGCGGCCGCTCCCTGCCCGGTCGACGTCAAGGCGAAGA
TGATCGAGTGGTGGGGACCGATCCTGATCGAGTACTACGCCGGCTCCGAGGGCAACGGCGTCACGGTGTCGACCTCGCAG
CAATGGCTCAGCCATCGCGGCACGGTCGGCAAGGCCGTGGTCGGAGTGATCAAGATCCTCGACGAGGCCGGCGAGGAGCT
GCCGACCGGCGAGATCGGCACCGTGTATTTCGCCGACGCGCCGGCGTTCAGCTATCACAACGATCCCGACAAGACAAAGA
ACGCTTACAACGACAAGGGCTGGTCCACGCTCGGCGACGTCGGCTATCTCGACGCCGAGGGCTTCCTCTATCTCACCGAC
CGCAAGAGCTACATGATCATCTCCGGCGGCGTGAACATCTATCCGCAGGAGACCGAGGACGTGCTGTTGACGCATCCCGA
CGTCGCCGACGTCGCGGTGTTCGGCGTGCCGAACGAGGAGATGGGCGAGGAGGTCAAGGCCGTAGTGCAGACCCGCGACG
GCGTCACGCCGAGCAAGGAGCTCGAGGCCAAACTGATCGGCTTCTGCCGCGACCGGCTCTCCGCGATCAAATGTCCGCGC
TCCATCGACTTCGAAGCAGAGCTGCCGCGCACGCCGACCGGCAAGCTGGTCAAGCGCCATCTGAAGGATCGCTACTGGCC
GAAGAAGCAGGCCGCGATGGCCTAA

Upstream 100 bases:

>100_bases
GGTTCGAGACGCGCGCCAACAGGCGCGCTCCTCACCATGAGGCGTCGCACCAAGTCAAAATGAGCGGAAAAATCCGCCGC
ATCACCACCGAGGACACGCC

Downstream 100 bases:

>100_bases
TCGGCGCGCTCCCTCGATGAATCGTCGCGGGAACGGCGCTGCCGCACCGGACCGCGCAAGGTCGGGGCTGCGACCAAGAT
AACGAGTCCCGCGGGCGCGC

Product: long-chain-fatty-acid--CoA ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MTHPSIHARTQPDKIAYQMASTGKAITYRELDELSNQGAQLFRTLGLKAGDHVALLMENRLAFMEICWAAQRAGLYYTAI
SRYLKQDEIAYIVQDCGARVVITSPQGADAIAPLVSTLPGVAFFMVDEPAPGFRSWDKEAGAQPTTPIADEVAGYDMLYS
SGTTGRPKGIKREFEGNAIDVPSPFLRLLCERMCGMNQDSIYLSPAPLYHAAPLRFNMMATTLGGTSVIMEHFDAEQFLA
LVEKYKVTQSQLVPTMFVRMLKLPDEVRTRYDVSSLKGAIHAAAPCPVDVKAKMIEWWGPILIEYYAGSEGNGVTVSTSQ
QWLSHRGTVGKAVVGVIKILDEAGEELPTGEIGTVYFADAPAFSYHNDPDKTKNAYNDKGWSTLGDVGYLDAEGFLYLTD
RKSYMIISGGVNIYPQETEDVLLTHPDVADVAVFGVPNEEMGEEVKAVVQTRDGVTPSKELEAKLIGFCRDRLSAIKCPR
SIDFEAELPRTPTGKLVKRHLKDRYWPKKQAAMA

Sequences:

>Translated_514_residues
MTHPSIHARTQPDKIAYQMASTGKAITYRELDELSNQGAQLFRTLGLKAGDHVALLMENRLAFMEICWAAQRAGLYYTAI
SRYLKQDEIAYIVQDCGARVVITSPQGADAIAPLVSTLPGVAFFMVDEPAPGFRSWDKEAGAQPTTPIADEVAGYDMLYS
SGTTGRPKGIKREFEGNAIDVPSPFLRLLCERMCGMNQDSIYLSPAPLYHAAPLRFNMMATTLGGTSVIMEHFDAEQFLA
LVEKYKVTQSQLVPTMFVRMLKLPDEVRTRYDVSSLKGAIHAAAPCPVDVKAKMIEWWGPILIEYYAGSEGNGVTVSTSQ
QWLSHRGTVGKAVVGVIKILDEAGEELPTGEIGTVYFADAPAFSYHNDPDKTKNAYNDKGWSTLGDVGYLDAEGFLYLTD
RKSYMIISGGVNIYPQETEDVLLTHPDVADVAVFGVPNEEMGEEVKAVVQTRDGVTPSKELEAKLIGFCRDRLSAIKCPR
SIDFEAELPRTPTGKLVKRHLKDRYWPKKQAAMA
>Mature_513_residues
THPSIHARTQPDKIAYQMASTGKAITYRELDELSNQGAQLFRTLGLKAGDHVALLMENRLAFMEICWAAQRAGLYYTAIS
RYLKQDEIAYIVQDCGARVVITSPQGADAIAPLVSTLPGVAFFMVDEPAPGFRSWDKEAGAQPTTPIADEVAGYDMLYSS
GTTGRPKGIKREFEGNAIDVPSPFLRLLCERMCGMNQDSIYLSPAPLYHAAPLRFNMMATTLGGTSVIMEHFDAEQFLAL
VEKYKVTQSQLVPTMFVRMLKLPDEVRTRYDVSSLKGAIHAAAPCPVDVKAKMIEWWGPILIEYYAGSEGNGVTVSTSQQ
WLSHRGTVGKAVVGVIKILDEAGEELPTGEIGTVYFADAPAFSYHNDPDKTKNAYNDKGWSTLGDVGYLDAEGFLYLTDR
KSYMIISGGVNIYPQETEDVLLTHPDVADVAVFGVPNEEMGEEVKAVVQTRDGVTPSKELEAKLIGFCRDRLSAIKCPRS
IDFEAELPRTPTGKLVKRHLKDRYWPKKQAAMA

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=542, Percent_Identity=25.830258302583, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI115511026, Length=499, Percent_Identity=27.0541082164329, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI187761345, Length=536, Percent_Identity=26.1194029850746, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI187761343, Length=536, Percent_Identity=26.1194029850746, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI122937307, Length=508, Percent_Identity=26.5748031496063, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI38505220, Length=532, Percent_Identity=25.7518796992481, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI157311624, Length=531, Percent_Identity=24.105461393597, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI157311622, Length=531, Percent_Identity=24.105461393597, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI58082049, Length=531, Percent_Identity=24.2937853107345, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI42544132, Length=508, Percent_Identity=26.1811023622047, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI28416953, Length=560, Percent_Identity=24.1071428571429, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI227499619, Length=465, Percent_Identity=24.9462365591398, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI40807357, Length=539, Percent_Identity=25.0463821892393, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI13375727, Length=540, Percent_Identity=24.4444444444444, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI62865631, Length=556, Percent_Identity=23.9208633093525, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI13325055, Length=556, Percent_Identity=23.9208633093525, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI38524616, Length=509, Percent_Identity=25.147347740668, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI8923896, Length=392, Percent_Identity=25.765306122449, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI253970489, Length=392, Percent_Identity=25.765306122449, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI13236579, Length=515, Percent_Identity=22.5242718446602, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI221142682, Length=514, Percent_Identity=28.2101167315175, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1788107, Length=525, Percent_Identity=28.7619047619048, Blast_Score=168, Evalue=6e-43,
Organism=Escherichia coli, GI145693145, Length=512, Percent_Identity=30.078125, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1786810, Length=517, Percent_Identity=24.1779497098646, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1790505, Length=536, Percent_Identity=23.5074626865672, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1786529, Length=556, Percent_Identity=21.4028776978417, Blast_Score=73, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17558820, Length=507, Percent_Identity=28.4023668639053, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17531443, Length=531, Percent_Identity=27.1186440677966, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI32563687, Length=508, Percent_Identity=26.9685039370079, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17559526, Length=522, Percent_Identity=26.8199233716475, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71994703, Length=541, Percent_Identity=26.6173752310536, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71994690, Length=541, Percent_Identity=26.6173752310536, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71994694, Length=541, Percent_Identity=26.6173752310536, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17557194, Length=523, Percent_Identity=27.3422562141491, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17560308, Length=522, Percent_Identity=25.095785440613, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17538037, Length=522, Percent_Identity=27.0114942528736, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI115534440, Length=554, Percent_Identity=26.5342960288809, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17560140, Length=495, Percent_Identity=24.040404040404, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71996755, Length=505, Percent_Identity=24.3564356435644, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71985884, Length=374, Percent_Identity=22.192513368984, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=538, Percent_Identity=24.907063197026, Blast_Score=126, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6323182, Length=563, Percent_Identity=23.4458259325044, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6319264, Length=556, Percent_Identity=22.6618705035971, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648260, Length=518, Percent_Identity=26.2548262548263, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24581924, Length=532, Percent_Identity=26.3157894736842, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI21355181, Length=488, Percent_Identity=26.844262295082, Blast_Score=140, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24648253, Length=473, Percent_Identity=24.7357293868922, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24648255, Length=473, Percent_Identity=24.9471458773784, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI21356441, Length=454, Percent_Identity=23.7885462555066, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24656500, Length=515, Percent_Identity=24.0776699029126, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI19922652, Length=508, Percent_Identity=24.8031496062992, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21358303, Length=465, Percent_Identity=23.6559139784946, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI281365686, Length=477, Percent_Identity=22.8511530398323, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI21356947, Length=516, Percent_Identity=23.4496124031008, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24653035, Length=364, Percent_Identity=26.6483516483516, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI18859661, Length=516, Percent_Identity=24.6124031007752, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI161076582, Length=366, Percent_Identity=25.4098360655738, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648257, Length=515, Percent_Identity=24.2718446601942, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24667955, Length=553, Percent_Identity=23.3273056057866, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI62472339, Length=537, Percent_Identity=23.2774674115456, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI21355225, Length=539, Percent_Identity=24.4897959183673, Blast_Score=102, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24667959, Length=369, Percent_Identity=25.2032520325203, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI20130357, Length=354, Percent_Identity=25.4237288135593, Blast_Score=88, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 56672; Mature: 56541

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHPSIHARTQPDKIAYQMASTGKAITYRELDELSNQGAQLFRTLGLKAGDHVALLMENR
CCCCCCCCCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
LAFMEICWAAQRAGLYYTAISRYLKQDEIAYIVQDCGARVVITSPQGADAIAPLVSTLPG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCC
VAFFMVDEPAPGFRSWDKEAGAQPTTPIADEVAGYDMLYSSGTTGRPKGIKREFEGNAID
EEEEEECCCCCCCCCHHHHCCCCCCCCCHHHHCCCHHEECCCCCCCCCCCCHHCCCCEEC
VPSPFLRLLCERMCGMNQDSIYLSPAPLYHAAPLRFNMMATTLGGTSVIMEHFDAEQFLA
CCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHCCHHHHHH
LVEKYKVTQSQLVPTMFVRMLKLPDEVRTRYDVSSLKGAIHAAAPCPVDVKAKMIEWWGP
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHHHHCC
ILIEYYAGSEGNGVTVSTSQQWLSHRGTVGKAVVGVIKILDEAGEELPTGEIGTVYFADA
EEEEEECCCCCCCEEEECHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECC
PAFSYHNDPDKTKNAYNDKGWSTLGDVGYLDAEGFLYLTDRKSYMIISGGVNIYPQETED
CCCCCCCCCCHHCCCCCCCCCCCCCCCCEECCCCEEEEECCCCEEEEECCCEECCCCCCC
VLLTHPDVADVAVFGVPNEEMGEEVKAVVQTRDGVTPSKELEAKLIGFCRDRLSAIKCPR
EEEECCCHHHEEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
SIDFEAELPRTPTGKLVKRHLKDRYWPKKQAAMA
CCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHCCC
>Mature Secondary Structure 
THPSIHARTQPDKIAYQMASTGKAITYRELDELSNQGAQLFRTLGLKAGDHVALLMENR
CCCCCCCCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
LAFMEICWAAQRAGLYYTAISRYLKQDEIAYIVQDCGARVVITSPQGADAIAPLVSTLPG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCC
VAFFMVDEPAPGFRSWDKEAGAQPTTPIADEVAGYDMLYSSGTTGRPKGIKREFEGNAID
EEEEEECCCCCCCCCHHHHCCCCCCCCCHHHHCCCHHEECCCCCCCCCCCCHHCCCCEEC
VPSPFLRLLCERMCGMNQDSIYLSPAPLYHAAPLRFNMMATTLGGTSVIMEHFDAEQFLA
CCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHCCHHHHHH
LVEKYKVTQSQLVPTMFVRMLKLPDEVRTRYDVSSLKGAIHAAAPCPVDVKAKMIEWWGP
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHHHHCC
ILIEYYAGSEGNGVTVSTSQQWLSHRGTVGKAVVGVIKILDEAGEELPTGEIGTVYFADA
EEEEEECCCCCCCEEEECHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECC
PAFSYHNDPDKTKNAYNDKGWSTLGDVGYLDAEGFLYLTDRKSYMIISGGVNIYPQETED
CCCCCCCCCCHHCCCCCCCCCCCCCCCCEECCCCEEEEECCCCEEEEECCCEECCCCCCC
VLLTHPDVADVAVFGVPNEEMGEEVKAVVQTRDGVTPSKELEAKLIGFCRDRLSAIKCPR
EEEECCCHHHEEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
SIDFEAELPRTPTGKLVKRHLKDRYWPKKQAAMA
CCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]