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The map label for this gene is gatA [H]

Identifier: 86748802

GI number: 86748802

Start: 1910476

End: 1911978

Strand: Reverse

Name: gatA [H]

Synonym: RPB_1679

Alternate gene names: 86748802

Gene position: 1911978-1910476 (Counterclockwise)

Preceding gene: 86748803

Following gene: 86748801

Centisome position: 35.86

GC content: 69.39

Gene sequence:

>1503_bases
ATGCGCCGCCGCAACAAAGGGAGTCCGGCTTTGACCGCATCACAATGGAGCTTCGCGACCGCCACCGAACTCAGCGCCGC
GCTGAAGGCGAAGAAGGTCTCTTCCGTCGAACTGACGCAGGACGCCATCGCCCGCATCGAGCGGCACGACGGTCAGATCA
ACGCGATCTGCGTGCGCGACTTCGACCGGGCCCTGCAGACCGCCCGCGCCGCCGACCTCGCGCTGTCGCGCGGCGGCCGC
GGATCGCTGCTCGGGATTCCGATGACGGTGAAGGAGAGCTTCAATGTCGCGGGCCTGCCGACGACGTGGGGCTTCGTCCC
GCACAAGGATTTCGTGGCGCAGGACGACGCACTGGCGATCGAGCGCGTCAAGGCCGCCGGCGGCGTGATCCTCGGCAAGA
CCAACGTGCCGGTCGCGCTCGGCGACTGGCAGAGCACCAACGAGATCTACGGCACGACCAACAATCCGTTCGACCTCGGC
CGCACCCCGGGCGGATCTTCCGGTGGATCGTCGGCCGCGCTCGCGGCCGGCTACGGCGCGCTGTCGCTCGGCTCCGACAT
CGGCGGCTCGCTGCGGGTGCCGGCGCATTATTGCGGCGTCTACGCGCACAAGCCGACCTTCGGGCTGTGCCCGGCGCGCG
GCCACACGCCGCCGCCGCTGCCGCCGCTGCCCTCCAACCGCGACCTGTCGGTGATCGGGCCGATGGCACGCTCGGCCACC
GACCTCGCGCTGCTGCTCGAGGTGATGGCGGGACCCGATCCGCTCTTCGACGGCATCGGCTACAAGCTCGATTTGCCCGC
GCCGCGCCATGCCGCGCTGAAGGATTTTCGCGTGCTGCTGCTCGACAGCCATCCGCTGCTGCCGACCGGCGCGAGCGTGC
GCGGCGCGATCGCGGCGCTCGAATCCGGACTCACCAAGGCCGGCGTCCGCGTCACGCGCACAACGCCGCTGCTGCCCGAT
CTCGCCGAGACTTCGCGCGTCTATATGCGGATGCTGCTGTCGTTCCTCGCGGCGGGCTTCCCGCCTGAGATTCTCGCCGA
AGTCCAGGGCGCCGTGACCGGGCTCGATGCCGCAGACGACAGCCTCCCCGCCGAGCGGCTGCGCGGCATGGTGCTGAGCC
ATCGCGACTGGGTGATCGCAGATGGCCGCCGCACCGGCCTGCGCGCGCAATGGCACGAGCTGTTCAAGCAGTTCGACGCG
GTGATCTGTCCGCTGATGCCGACGCCGGCCTATCCGCACGACCATCACGAGCCGCAGGAAGAGCGCCGGATCCAGATCGA
CGGCAAGCCCTACCCTTACGTCGATCAGCTGGTCTGGCCAGGCATCGCCACGCTGCCCGGCCTGCCGGCGACGGCGCTGC
CGATCGCCATGTCCGCTGAAGGTCTGCCGATCGGCGTGCAGATCGTCGGCCCGTGGCTGGAAGATCGCACGCCGCTGAAA
CTCGCCGAACTGATCGAGCGCGAATTCGGCGGCTTCAAAGCCCCACCGGCGTTCGTGAGTTAG

Upstream 100 bases:

>100_bases
ATCCCGTCTCGCGGGATTCGCTATCAAAATGTCGCCGGCCGCTTCTCGCTTTGCACCGCTCGTGCCCCGCCGAATTGCAA
TTCGTACCGCCGTCGTCATG

Downstream 100 bases:

>100_bases
ATCGCGCTCGCAGCACGCGCCGCCATGGTCATCATGGCGGCGCGGCGATCGACCAACACCCGCGGCTGCTGTGGCGAGGG
CACAGCTGGAGACAAACGAT

Product: amidase

Products: monocarboxylate; NH3

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MRRRNKGSPALTASQWSFATATELSAALKAKKVSSVELTQDAIARIERHDGQINAICVRDFDRALQTARAADLALSRGGR
GSLLGIPMTVKESFNVAGLPTTWGFVPHKDFVAQDDALAIERVKAAGGVILGKTNVPVALGDWQSTNEIYGTTNNPFDLG
RTPGGSSGGSSAALAAGYGALSLGSDIGGSLRVPAHYCGVYAHKPTFGLCPARGHTPPPLPPLPSNRDLSVIGPMARSAT
DLALLLEVMAGPDPLFDGIGYKLDLPAPRHAALKDFRVLLLDSHPLLPTGASVRGAIAALESGLTKAGVRVTRTTPLLPD
LAETSRVYMRMLLSFLAAGFPPEILAEVQGAVTGLDAADDSLPAERLRGMVLSHRDWVIADGRRTGLRAQWHELFKQFDA
VICPLMPTPAYPHDHHEPQEERRIQIDGKPYPYVDQLVWPGIATLPGLPATALPIAMSAEGLPIGVQIVGPWLEDRTPLK
LAELIEREFGGFKAPPAFVS

Sequences:

>Translated_500_residues
MRRRNKGSPALTASQWSFATATELSAALKAKKVSSVELTQDAIARIERHDGQINAICVRDFDRALQTARAADLALSRGGR
GSLLGIPMTVKESFNVAGLPTTWGFVPHKDFVAQDDALAIERVKAAGGVILGKTNVPVALGDWQSTNEIYGTTNNPFDLG
RTPGGSSGGSSAALAAGYGALSLGSDIGGSLRVPAHYCGVYAHKPTFGLCPARGHTPPPLPPLPSNRDLSVIGPMARSAT
DLALLLEVMAGPDPLFDGIGYKLDLPAPRHAALKDFRVLLLDSHPLLPTGASVRGAIAALESGLTKAGVRVTRTTPLLPD
LAETSRVYMRMLLSFLAAGFPPEILAEVQGAVTGLDAADDSLPAERLRGMVLSHRDWVIADGRRTGLRAQWHELFKQFDA
VICPLMPTPAYPHDHHEPQEERRIQIDGKPYPYVDQLVWPGIATLPGLPATALPIAMSAEGLPIGVQIVGPWLEDRTPLK
LAELIEREFGGFKAPPAFVS
>Mature_500_residues
MRRRNKGSPALTASQWSFATATELSAALKAKKVSSVELTQDAIARIERHDGQINAICVRDFDRALQTARAADLALSRGGR
GSLLGIPMTVKESFNVAGLPTTWGFVPHKDFVAQDDALAIERVKAAGGVILGKTNVPVALGDWQSTNEIYGTTNNPFDLG
RTPGGSSGGSSAALAAGYGALSLGSDIGGSLRVPAHYCGVYAHKPTFGLCPARGHTPPPLPPLPSNRDLSVIGPMARSAT
DLALLLEVMAGPDPLFDGIGYKLDLPAPRHAALKDFRVLLLDSHPLLPTGASVRGAIAALESGLTKAGVRVTRTTPLLPD
LAETSRVYMRMLLSFLAAGFPPEILAEVQGAVTGLDAADDSLPAERLRGMVLSHRDWVIADGRRTGLRAQWHELFKQFDA
VICPLMPTPAYPHDHHEPQEERRIQIDGKPYPYVDQLVWPGIATLPGLPATALPIAMSAEGLPIGVQIVGPWLEDRTPLK
LAELIEREFGGFKAPPAFVS

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=514, Percent_Identity=28.5992217898833, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI222831590, Length=523, Percent_Identity=25.2390057361377, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI166795287, Length=127, Percent_Identity=37.7952755905512, Blast_Score=73, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17537465, Length=510, Percent_Identity=28.0392156862745, Blast_Score=161, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI71990152, Length=249, Percent_Identity=30.5220883534137, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17538252, Length=253, Percent_Identity=33.201581027668, Blast_Score=112, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17556264, Length=229, Percent_Identity=32.7510917030568, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17556276, Length=195, Percent_Identity=37.4358974358974, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17556278, Length=195, Percent_Identity=37.4358974358974, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17538254, Length=530, Percent_Identity=24.3396226415094, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17543272, Length=174, Percent_Identity=33.9080459770115, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6320448, Length=253, Percent_Identity=30.0395256916996, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6319685, Length=421, Percent_Identity=26.603325415677, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45550774, Length=499, Percent_Identity=29.6593186372746, Blast_Score=193, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24648435, Length=499, Percent_Identity=29.8597194388778, Blast_Score=193, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24648437, Length=499, Percent_Identity=29.8597194388778, Blast_Score=193, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24644968, Length=508, Percent_Identity=29.7244094488189, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24648441, Length=429, Percent_Identity=30.5361305361305, Blast_Score=170, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24648439, Length=429, Percent_Identity=30.5361305361305, Blast_Score=170, Evalue=3e-42,
Organism=Drosophila melanogaster, GI161078093, Length=508, Percent_Identity=27.9527559055118, Blast_Score=158, Evalue=9e-39,
Organism=Drosophila melanogaster, GI21356731, Length=539, Percent_Identity=26.3450834879406, Blast_Score=152, Evalue=6e-37,
Organism=Drosophila melanogaster, GI24652985, Length=255, Percent_Identity=33.7254901960784, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19922090, Length=255, Percent_Identity=33.7254901960784, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24652981, Length=255, Percent_Identity=33.7254901960784, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24652983, Length=255, Percent_Identity=33.7254901960784, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24648113, Length=507, Percent_Identity=28.5996055226824, Blast_Score=103, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 3.5.1.4

Molecular weight: Translated: 53245; Mature: 53245

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRRNKGSPALTASQWSFATATELSAALKAKKVSSVELTQDAIARIERHDGQINAICVRD
CCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEHH
FDRALQTARAADLALSRGGRGSLLGIPMTVKESFNVAGLPTTWGFVPHKDFVAQDDALAI
HHHHHHHHHHHHHHHCCCCCCCEEECCEEHHCCCCCCCCCCCCCCCCCHHHHCCCCHHHH
ERVKAAGGVILGKTNVPVALGDWQSTNEIYGTTNNPFDLGRTPGGSSGGSSAALAAGYGA
HHHHHCCCEEEECCCCCEEECCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCHHHCCCH
LSLGSDIGGSLRVPAHYCGVYAHKPTFGLCPARGHTPPPLPPLPSNRDLSVIGPMARSAT
HCCCCCCCCCEECCHHHHCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHH
DLALLLEVMAGPDPLFDGIGYKLDLPAPRHAALKDFRVLLLDSHPLLPTGASVRGAIAAL
HHHHHHHHHCCCCHHHHCCCCEEECCCCCHHHHHHEEEEEECCCCCCCCCCHHHHHHHHH
ESGLTKAGVRVTRTTPLLPDLAETSRVYMRMLLSFLAAGFPPEILAEVQGAVTGLDAADD
HHCHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
SLPAERLRGMVLSHRDWVIADGRRTGLRAQWHELFKQFDAVICPLMPTPAYPHDHHEPQE
CCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCH
ERRIQIDGKPYPYVDQLVWPGIATLPGLPATALPIAMSAEGLPIGVQIVGPWLEDRTPLK
HCEEEECCCCCCHHHHHHCCCHHHCCCCCHHHCCEEECCCCCEEEEEEECCCCCCCCCHH
LAELIEREFGGFKAPPAFVS
HHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MRRRNKGSPALTASQWSFATATELSAALKAKKVSSVELTQDAIARIERHDGQINAICVRD
CCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEHH
FDRALQTARAADLALSRGGRGSLLGIPMTVKESFNVAGLPTTWGFVPHKDFVAQDDALAI
HHHHHHHHHHHHHHHCCCCCCCEEECCEEHHCCCCCCCCCCCCCCCCCHHHHCCCCHHHH
ERVKAAGGVILGKTNVPVALGDWQSTNEIYGTTNNPFDLGRTPGGSSGGSSAALAAGYGA
HHHHHCCCEEEECCCCCEEECCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCHHHCCCH
LSLGSDIGGSLRVPAHYCGVYAHKPTFGLCPARGHTPPPLPPLPSNRDLSVIGPMARSAT
HCCCCCCCCCEECCHHHHCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHH
DLALLLEVMAGPDPLFDGIGYKLDLPAPRHAALKDFRVLLLDSHPLLPTGASVRGAIAAL
HHHHHHHHHCCCCHHHHCCCCEEECCCCCHHHHHHEEEEEECCCCCCCCCCHHHHHHHHH
ESGLTKAGVRVTRTTPLLPDLAETSRVYMRMLLSFLAAGFPPEILAEVQGAVTGLDAADD
HHCHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
SLPAERLRGMVLSHRDWVIADGRRTGLRAQWHELFKQFDAVICPLMPTPAYPHDHHEPQE
CCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCH
ERRIQIDGKPYPYVDQLVWPGIATLPGLPATALPIAMSAEGLPIGVQIVGPWLEDRTPLK
HCEEEECCCCCCHHHHHHCCCHHHCCCCCHHHCCEEECCCCCEEEEEEECCCCCCCCCHH
LAELIEREFGGFKAPPAFVS
HHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: monocarboxylic acid amide; H2O

Specific reaction: A monocarboxylic acid amide + H2O = a monocarboxylate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA