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The map label for this gene is ycjG [H]

Identifier: 86748750

GI number: 86748750

Start: 1832529

End: 1833524

Strand: Reverse

Name: ycjG [H]

Synonym: RPB_1626

Alternate gene names: 86748750

Gene position: 1833524-1832529 (Counterclockwise)

Preceding gene: 86748754

Following gene: 86748748

Centisome position: 34.39

GC content: 72.79

Gene sequence:

>996_bases
ATGACGTCCATCAATCCGACCCAATTGACTGCACGCATCGAGCATTGGCCGATCGCCGGAGCCTTCACCATCAGCCGCGG
CGCCAAGACCGAAGCGGTGGTCGTGACGGCCGAGATCAGGCGTAACGGACAAGCCGGCCGCGGCGAATGCGTTCCTTACG
CCCGCTACGGCGAGACGCCGGAGACCACCGTCGCCGCGATCGAGGCGATGCGCGCGCCGCTGCGTCAGGGGCTCGACCGC
GCCGGCCTGCAATCGGCGATGCCGCCGGGCGCGGCGCGCAATGCGCTGGATTGCGCGCTGCTCGACCTCGAGGCCGGGCT
GAGCGGACGCCGCGCCTGGGAGCTGCTCGGCAGCGCCGCGCCGCAACCGGCGACCACCGCCTACACCATTTCGCTGGGCA
CGCCGGAGGCGATGGCGGAGGCGACCGCGAAAGCCGCCGCCCGCCCGCTGCTGAAGATCAAGCTCGGCGGCGACGGCGAC
GATGCCCGGATCGCCGCGGTCCGCCGCGCCGCGCCGCGAGCCGAACTGATCGTCGACGCCAACGAGGCCTGGACGCCGGA
CAATCTCGCCCGCAACCTCGCCGCCTGCGCCGGCGCCGGCGTGACGCTGGTGGAGCAGCCGCTGCCGGCCGGGCGCGACG
AGGCGCTGGCGCAAATCCGCCGGCCGATCGCGGTCTGCGCCGACGAGAGCGTCCACGCCCGCGCCTCGCTGGATGCCCTG
CGCGGCCGCTACGACGCCGTCAACATCAAGCTCGACAAGACCGGCGGCGTCACCGAGGCGATGGCGATGGCGGAGGCGGC
GCGCGCGCTCGGCCTCGACATCATGGTCGGCTGCATGGTCGCGACCTCGCTGTCGATGGCGCCGGCGATGCTGCTGACGC
CGCACGCCCGCTTCGTCGATCTCGACGGCCCGCTGCTGCTGGCGAAGGATCGCGACGACGGCCTGCGCTACGACGGCAGC
ATCGTCTATCCGCCGGAGCCCTCGTTGTGGGGCTGA

Upstream 100 bases:

>100_bases
GAGCCAATCCGGAGTTCAATACGACTTGTGAGGAAGGTGCACTACCAGCCATACTTAGTTGCGCCGGGCAACCCTCGGTT
TGAGTTTGCCAGAAATCCCA

Downstream 100 bases:

>100_bases
TCGTGCGTGTCGAGGCGGCGACGCATCGCAACGACCACCACCGCGCCGATCAGCGCCACCGCCGCCATCAGATCGTAGAC
GGCGAGGCCCCAGCGCGCGA

Product: mandelate racemase/muconate lactonizing protein

Products: cis,cis-hexadienedioate

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTSINPTQLTARIEHWPIAGAFTISRGAKTEAVVVTAEIRRNGQAGRGECVPYARYGETPETTVAAIEAMRAPLRQGLDR
AGLQSAMPPGAARNALDCALLDLEAGLSGRRAWELLGSAAPQPATTAYTISLGTPEAMAEATAKAAARPLLKIKLGGDGD
DARIAAVRRAAPRAELIVDANEAWTPDNLARNLAACAGAGVTLVEQPLPAGRDEALAQIRRPIAVCADESVHARASLDAL
RGRYDAVNIKLDKTGGVTEAMAMAEAARALGLDIMVGCMVATSLSMAPAMLLTPHARFVDLDGPLLLAKDRDDGLRYDGS
IVYPPEPSLWG

Sequences:

>Translated_331_residues
MTSINPTQLTARIEHWPIAGAFTISRGAKTEAVVVTAEIRRNGQAGRGECVPYARYGETPETTVAAIEAMRAPLRQGLDR
AGLQSAMPPGAARNALDCALLDLEAGLSGRRAWELLGSAAPQPATTAYTISLGTPEAMAEATAKAAARPLLKIKLGGDGD
DARIAAVRRAAPRAELIVDANEAWTPDNLARNLAACAGAGVTLVEQPLPAGRDEALAQIRRPIAVCADESVHARASLDAL
RGRYDAVNIKLDKTGGVTEAMAMAEAARALGLDIMVGCMVATSLSMAPAMLLTPHARFVDLDGPLLLAKDRDDGLRYDGS
IVYPPEPSLWG
>Mature_330_residues
TSINPTQLTARIEHWPIAGAFTISRGAKTEAVVVTAEIRRNGQAGRGECVPYARYGETPETTVAAIEAMRAPLRQGLDRA
GLQSAMPPGAARNALDCALLDLEAGLSGRRAWELLGSAAPQPATTAYTISLGTPEAMAEATAKAAARPLLKIKLGGDGDD
ARIAAVRRAAPRAELIVDANEAWTPDNLARNLAACAGAGVTLVEQPLPAGRDEALAQIRRPIAVCADESVHARASLDALR
GRYDAVNIKLDKTGGVTEAMAMAEAARALGLDIMVGCMVATSLSMAPAMLLTPHARFVDLDGPLLLAKDRDDGLRYDGSI
VYPPEPSLWG

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has a role in the recycling of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of all the L-Ala-X dipeptides, excep

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=306, Percent_Identity=47.0588235294118, Blast_Score=263, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: 5.5.1.1

Molecular weight: Translated: 34690; Mature: 34559

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSINPTQLTARIEHWPIAGAFTISRGAKTEAVVVTAEIRRNGQAGRGECVPYARYGETP
CCCCCHHHEEEEEECCCCEEEEEECCCCCCEEEEEEEEHHCCCCCCCCCCCCHHHCCCCC
ETTVAAIEAMRAPLRQGLDRAGLQSAMPPGAARNALDCALLDLEAGLSGRRAWELLGSAA
HHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHEEEEEHHCCCCCHHHHHHHHCCC
PQPATTAYTISLGTPEAMAEATAKAAARPLLKIKLGGDGDDARIAAVRRAAPRAELIVDA
CCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCCEEEEEEC
NEAWTPDNLARNLAACAGAGVTLVEQPLPAGRDEALAQIRRPIAVCADESVHARASLDAL
CCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCEEECCCCHHHHHHHHHH
RGRYDAVNIKLDKTGGVTEAMAMAEAARALGLDIMVGCMVATSLSMAPAMLLTPHARFVD
CCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCEEEE
LDGPLLLAKDRDDGLRYDGSIVYPPEPSLWG
CCCCEEEEECCCCCCEECCEEEECCCCCCCC
>Mature Secondary Structure 
TSINPTQLTARIEHWPIAGAFTISRGAKTEAVVVTAEIRRNGQAGRGECVPYARYGETP
CCCCHHHEEEEEECCCCEEEEEECCCCCCEEEEEEEEHHCCCCCCCCCCCCHHHCCCCC
ETTVAAIEAMRAPLRQGLDRAGLQSAMPPGAARNALDCALLDLEAGLSGRRAWELLGSAA
HHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHEEEEEHHCCCCCHHHHHHHHCCC
PQPATTAYTISLGTPEAMAEATAKAAARPLLKIKLGGDGDDARIAAVRRAAPRAELIVDA
CCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCCEEEEEEC
NEAWTPDNLARNLAACAGAGVTLVEQPLPAGRDEALAQIRRPIAVCADESVHARASLDAL
CCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCEEECCCCHHHHHHHHHH
RGRYDAVNIKLDKTGGVTEAMAMAEAARALGLDIMVGCMVATSLSMAPAMLLTPHARFVD
CCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCEEEE
LDGPLLLAKDRDDGLRYDGSIVYPPEPSLWG
CCCCEEEEECCCCCCEECCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-dihydro-5-oxofuran-2-acetate

Specific reaction: 2,5-dihydro-5-oxofuran-2-acetate = cis,cis-hexadienedioate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7499381; 9097039; 9278503 [H]