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The map label for this gene is alkK [H]

Identifier: 86748290

GI number: 86748290

Start: 1335132

End: 1336760

Strand: Reverse

Name: alkK [H]

Synonym: RPB_1165

Alternate gene names: 86748290

Gene position: 1336760-1335132 (Counterclockwise)

Preceding gene: 86748292

Following gene: 86748289

Centisome position: 25.07

GC content: 64.21

Gene sequence:

>1629_bases
ATGCTTGGACTGATGCAAGACTGGCCTTTGCTGTGTCACCGGATCATCGAGCATGCGGCGCAGGTCCATGGCAACCAGGA
GGTGGTCACCCGTTCGGTGGAGGGGCCGATCGTCCGCACCACCTATGCGCAGATCCATCGTCGGGCGCTGAAGGTGAGCC
AGATGCTGGACCGCGCCGGCATCAAGCTCGGCGACCGCGTCGCGACGATCGCCTGGAACACCGCCCGGCATCTGGAATGC
TGGTACGGCATCATGGGAATCGGCGCGATCTGCCATACGGTCAATCCGCGGCTTTTTCCGGACCAGATCGTCTGGATCGT
CAATCATGCCCAGGACCGGGTGATGATCACCGATCTCACCTTTATCCCGATCCTCGAAAAGATCGCCGACAAGATGCCGA
GCGTGGAGCGCTACATCGTGCTCACCGATGCGGAACACATGCCGCAGACCACGCTGAAGAACGCGGTCGCCTACGAGGAA
TGGCTGAAGGAGGCCGACGGCGACTTCGCATGGAAGAGCTTCGACGAGAACACCGCGGCGGCGATGTGCTACACCTCGGG
CACCACCGGCGATCCCAAGGGCGTGCTGTATTCGCACCGCTCCAACGTGCTGCACGCGCTGATGGCCAACAACGGCGACT
CGCTCGGCACCCGCGCGTCCGACACCATGCTGCCGGTGGTGCCGCTGTTCCACGCCAATTCCTGGGGTATCGCGTTCTCG
GCGCCGTCGATGGGCACCAAGCTGGTGATGCCCGGCGCCAAGCTCGACGGCGCCTCGGTCTATGAACTGCTGTCGACCGA
GAAGGTGACGCACACGGCCGGTGTTCCCACGGTGTGGCTGATGCTGCTGCAATACATGGCCAAGGAAAAGCTGACGCTGC
CGGATCTGAGGATGGTGGTGTGCGGCGGCTCGGCGATGCCGCGCTCGATGATCAAGGCGTTCGTCGACATGGGCTGCGAG
GCGCGCCACGCCTGGGGCATGACCGAGATGAGCCCGATCGGCACGCTGGCGGCGCTGAAGCCGCCCTTCGACAAGCTCGA
AGGCGACGAGCGGCTCGACCGGCTGTCGACCCAGGGCTATCCGCCGTTCGGCGTGCAGATGAAGATCACCGACGATGCCG
GCACGGACGTGCCGTGGGACGGCAAGACCTTCGGCCGGCTCAAAGTGTCGGGCCCGGCGGTCGCGAAAGCGTACTACCGC
GTCGACACCGAGATCCTCGACGATCAGGGCTTCTTCGACACCGGCGACGTCGCCACCGTCGACGCCGACGCCTATATGCG
GATCACCGACCGCTCCAAGGACGTGATCAAGTCCGGCGGCGAGTGGATCTCGTCGATCGACCTGGAGAATCTCGCGGTCG
GCCACCCCAAGGTGGCGGAAGCCGCGGTGATCGGCGTGTATCACCCGAAATGGGACGAGCGGCCGCTCTTGATCTGCCAG
CTCAAGCCGGACGTCACCTGCACCCGCGACGAGATCCTGCAATACATGGACGGCAAGATCGCCAAATGGTGGATGCCCGA
CGACATCGTGTTCGTCGAAGCGATCCCGCATACGGCGACCGGCAAGATCCTGAAGACCGCCTTGCGCGATCAGTTCAAGA
CCTACAGCCTGCCGAACGCGGCGGCGTGA

Upstream 100 bases:

>100_bases
TGCCGCGGAACAATTTGCGACAAGACCGTTTTCGCCCGGCCGTCTCCGCGCTAAGGTCGCGCCCAATCCATGAAGGATAA
CAATCAGGAGGAACGATTTG

Downstream 100 bases:

>100_bases
GGACGGCTGTTTTCGCAAGGCGTCGCCACCGGCACGGTGCAACCTCTCCCGCTTGCGGGGGAGGTCGGATCGCGCAGCGA
TCCGGGTGGGGGAAGCGGCA

Product: putative acyl-CoA synthetase

Products: NA

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MLGLMQDWPLLCHRIIEHAAQVHGNQEVVTRSVEGPIVRTTYAQIHRRALKVSQMLDRAGIKLGDRVATIAWNTARHLEC
WYGIMGIGAICHTVNPRLFPDQIVWIVNHAQDRVMITDLTFIPILEKIADKMPSVERYIVLTDAEHMPQTTLKNAVAYEE
WLKEADGDFAWKSFDENTAAAMCYTSGTTGDPKGVLYSHRSNVLHALMANNGDSLGTRASDTMLPVVPLFHANSWGIAFS
APSMGTKLVMPGAKLDGASVYELLSTEKVTHTAGVPTVWLMLLQYMAKEKLTLPDLRMVVCGGSAMPRSMIKAFVDMGCE
ARHAWGMTEMSPIGTLAALKPPFDKLEGDERLDRLSTQGYPPFGVQMKITDDAGTDVPWDGKTFGRLKVSGPAVAKAYYR
VDTEILDDQGFFDTGDVATVDADAYMRITDRSKDVIKSGGEWISSIDLENLAVGHPKVAEAAVIGVYHPKWDERPLLICQ
LKPDVTCTRDEILQYMDGKIAKWWMPDDIVFVEAIPHTATGKILKTALRDQFKTYSLPNAAA

Sequences:

>Translated_542_residues
MLGLMQDWPLLCHRIIEHAAQVHGNQEVVTRSVEGPIVRTTYAQIHRRALKVSQMLDRAGIKLGDRVATIAWNTARHLEC
WYGIMGIGAICHTVNPRLFPDQIVWIVNHAQDRVMITDLTFIPILEKIADKMPSVERYIVLTDAEHMPQTTLKNAVAYEE
WLKEADGDFAWKSFDENTAAAMCYTSGTTGDPKGVLYSHRSNVLHALMANNGDSLGTRASDTMLPVVPLFHANSWGIAFS
APSMGTKLVMPGAKLDGASVYELLSTEKVTHTAGVPTVWLMLLQYMAKEKLTLPDLRMVVCGGSAMPRSMIKAFVDMGCE
ARHAWGMTEMSPIGTLAALKPPFDKLEGDERLDRLSTQGYPPFGVQMKITDDAGTDVPWDGKTFGRLKVSGPAVAKAYYR
VDTEILDDQGFFDTGDVATVDADAYMRITDRSKDVIKSGGEWISSIDLENLAVGHPKVAEAAVIGVYHPKWDERPLLICQ
LKPDVTCTRDEILQYMDGKIAKWWMPDDIVFVEAIPHTATGKILKTALRDQFKTYSLPNAAA
>Mature_542_residues
MLGLMQDWPLLCHRIIEHAAQVHGNQEVVTRSVEGPIVRTTYAQIHRRALKVSQMLDRAGIKLGDRVATIAWNTARHLEC
WYGIMGIGAICHTVNPRLFPDQIVWIVNHAQDRVMITDLTFIPILEKIADKMPSVERYIVLTDAEHMPQTTLKNAVAYEE
WLKEADGDFAWKSFDENTAAAMCYTSGTTGDPKGVLYSHRSNVLHALMANNGDSLGTRASDTMLPVVPLFHANSWGIAFS
APSMGTKLVMPGAKLDGASVYELLSTEKVTHTAGVPTVWLMLLQYMAKEKLTLPDLRMVVCGGSAMPRSMIKAFVDMGCE
ARHAWGMTEMSPIGTLAALKPPFDKLEGDERLDRLSTQGYPPFGVQMKITDDAGTDVPWDGKTFGRLKVSGPAVAKAYYR
VDTEILDDQGFFDTGDVATVDADAYMRITDRSKDVIKSGGEWISSIDLENLAVGHPKVAEAAVIGVYHPKWDERPLLICQ
LKPDVTCTRDEILQYMDGKIAKWWMPDDIVFVEAIPHTATGKILKTALRDQFKTYSLPNAAA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=538, Percent_Identity=26.0223048327138, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI187761343, Length=538, Percent_Identity=26.0223048327138, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI156151445, Length=534, Percent_Identity=26.0299625468165, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI28416953, Length=541, Percent_Identity=25.5083179297597, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI38505220, Length=516, Percent_Identity=24.4186046511628, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI157311624, Length=511, Percent_Identity=24.4618395303327, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI157311622, Length=511, Percent_Identity=24.4618395303327, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI58082049, Length=515, Percent_Identity=23.8834951456311, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI42544132, Length=526, Percent_Identity=23.1939163498099, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI115511026, Length=517, Percent_Identity=22.6305609284333, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI13375727, Length=570, Percent_Identity=21.9298245614035, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI253970489, Length=445, Percent_Identity=22.6966292134831, Blast_Score=69, Evalue=2e-11,
Organism=Homo sapiens, GI8923896, Length=571, Percent_Identity=22.0665499124343, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI145693145, Length=518, Percent_Identity=26.6409266409266, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI221142682, Length=532, Percent_Identity=24.0601503759398, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1788107, Length=518, Percent_Identity=25.0965250965251, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI1786810, Length=513, Percent_Identity=23.9766081871345, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1789201, Length=368, Percent_Identity=25.5434782608696, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1788595, Length=532, Percent_Identity=20.1127819548872, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790505, Length=181, Percent_Identity=30.939226519337, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17558820, Length=506, Percent_Identity=24.901185770751, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17531443, Length=541, Percent_Identity=23.4750462107209, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI32563687, Length=506, Percent_Identity=24.3083003952569, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71994703, Length=554, Percent_Identity=24.187725631769, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71994694, Length=554, Percent_Identity=24.187725631769, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71994690, Length=554, Percent_Identity=24.187725631769, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17538037, Length=543, Percent_Identity=23.7569060773481, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17559526, Length=533, Percent_Identity=24.015009380863, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17557194, Length=543, Percent_Identity=23.7569060773481, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17560308, Length=536, Percent_Identity=23.5074626865672, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI71996755, Length=523, Percent_Identity=22.9445506692161, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71985884, Length=376, Percent_Identity=25.531914893617, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI115534440, Length=523, Percent_Identity=21.0325047801147, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17560140, Length=364, Percent_Identity=22.8021978021978, Blast_Score=76, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=363, Percent_Identity=28.3746556473829, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24581924, Length=564, Percent_Identity=26.063829787234, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI18859661, Length=514, Percent_Identity=26.0700389105058, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI21355181, Length=538, Percent_Identity=24.1635687732342, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI62472339, Length=567, Percent_Identity=23.4567901234568, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24667955, Length=566, Percent_Identity=23.321554770318, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24667959, Length=469, Percent_Identity=24.3070362473348, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648260, Length=509, Percent_Identity=24.5579567779961, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI21356947, Length=522, Percent_Identity=22.2222222222222, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648255, Length=531, Percent_Identity=22.2222222222222, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648257, Length=520, Percent_Identity=21.9230769230769, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24648253, Length=531, Percent_Identity=22.0338983050847, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19922652, Length=504, Percent_Identity=20.0396825396825, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI281365686, Length=365, Percent_Identity=21.0958904109589, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: NA

Molecular weight: Translated: 60022; Mature: 60022

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGLMQDWPLLCHRIIEHAAQVHGNQEVVTRSVEGPIVRTTYAQIHRRALKVSQMLDRAG
CCCCCCCHHHHHHHHHHHHHHHCCCHHHEEECCCCCEEEHHHHHHHHHHHHHHHHHHHCC
IKLGDRVATIAWNTARHLECWYGIMGIGAICHTVNPRLFPDQIVWIVNHAQDRVMITDLT
CCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEECCH
FIPILEKIADKMPSVERYIVLTDAEHMPQTTLKNAVAYEEWLKEADGDFAWKSFDENTAA
HHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCE
AMCYTSGTTGDPKGVLYSHRSNVLHALMANNGDSLGTRASDTMLPVVPLFHANSWGIAFS
EEEEECCCCCCCCCEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCEEEE
APSMGTKLVMPGAKLDGASVYELLSTEKVTHTAGVPTVWLMLLQYMAKEKLTLPDLRMVV
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
CGGSAMPRSMIKAFVDMGCEARHAWGMTEMSPIGTLAALKPPFDKLEGDERLDRLSTQGY
ECCCCCHHHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHCCCCHHHCCCHHHHHHHHCCCC
PPFGVQMKITDDAGTDVPWDGKTFGRLKVSGPAVAKAYYRVDTEILDDQGFFDTGDVATV
CCCCEEEEEECCCCCCCCCCCCCCCEEEECCCHHHHHHHEECHHHCCCCCCCCCCCEEEE
DADAYMRITDRSKDVIKSGGEWISSIDLENLAVGHPKVAEAAVIGVYHPKWDERPLLICQ
CCCEEEEEECCCHHHHHCCCHHHHHCCCCCEECCCCCHHCEEEEEEECCCCCCCCEEEEE
LKPDVTCTRDEILQYMDGKIAKWWMPDDIVFVEAIPHTATGKILKTALRDQFKTYSLPNA
ECCCCCCCHHHHHHHHCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCC
AA
CC
>Mature Secondary Structure
MLGLMQDWPLLCHRIIEHAAQVHGNQEVVTRSVEGPIVRTTYAQIHRRALKVSQMLDRAG
CCCCCCCHHHHHHHHHHHHHHHCCCHHHEEECCCCCEEEHHHHHHHHHHHHHHHHHHHCC
IKLGDRVATIAWNTARHLECWYGIMGIGAICHTVNPRLFPDQIVWIVNHAQDRVMITDLT
CCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEECCH
FIPILEKIADKMPSVERYIVLTDAEHMPQTTLKNAVAYEEWLKEADGDFAWKSFDENTAA
HHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCE
AMCYTSGTTGDPKGVLYSHRSNVLHALMANNGDSLGTRASDTMLPVVPLFHANSWGIAFS
EEEEECCCCCCCCCEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCEEEE
APSMGTKLVMPGAKLDGASVYELLSTEKVTHTAGVPTVWLMLLQYMAKEKLTLPDLRMVV
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
CGGSAMPRSMIKAFVDMGCEARHAWGMTEMSPIGTLAALKPPFDKLEGDERLDRLSTQGY
ECCCCCHHHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHCCCCHHHCCCHHHHHHHHCCCC
PPFGVQMKITDDAGTDVPWDGKTFGRLKVSGPAVAKAYYRVDTEILDDQGFFDTGDVATV
CCCCEEEEEECCCCCCCCCCCCCCCEEEECCCHHHHHHHEECHHHCCCCCCCCCCCEEEE
DADAYMRITDRSKDVIKSGGEWISSIDLENLAVGHPKVAEAAVIGVYHPKWDERPLLICQ
CCCEEEEEECCCHHHHHCCCHHHHHCCCCCEECCCCCHHCEEEEEEECCCCCCCCEEEEE
LKPDVTCTRDEILQYMDGKIAKWWMPDDIVFVEAIPHTATGKILKTALRDQFKTYSLPNA
ECCCCCCCHHHHHHHHCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCC
AA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]