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The map label for this gene is nadB [H]

Identifier: 86748231

GI number: 86748231

Start: 1272598

End: 1274220

Strand: Reverse

Name: nadB [H]

Synonym: RPB_1106

Alternate gene names: 86748231

Gene position: 1274220-1272598 (Counterclockwise)

Preceding gene: 86748232

Following gene: 86748230

Centisome position: 23.9

GC content: 72.77

Gene sequence:

>1623_bases
ATGGGTCTCTCAACCGAAATCGAATCGCTCGGCGCCCATGGTGACATCGTGATCGTCGGCGGTGGCCTCGCCGGCCTGTT
CTGCGCGCTGAAGCTGGCACCGCGGCCGGTGACGGTGATCACCGCGGCGGCGCTCGGCGAAGGCGCCTCGACCGCCTGGG
CGCAAGGCGGCATCGCGGCTGCGGTCGGCGAAGGCGACAGCGCCGAATCCCACGCGGCCGACACGATCGCGGTCGGCGCC
GGCCTCGTGGACGAGGCGGTGGCGCTCGGCCTCGCGCAGGAAGCCTCCGCCCGCATCCACGATCTGCTCGCCTATGGCGT
GCCGTTCGATCGCGACCTCGAAGGCAGGCTCGCGGTGGCGCGCGAGGCAGCGCATTCGGCACGGCGGATCGTGCACGTTC
GCGGCGACATGGCCGGCAAGGCGATCATCACCGCGCTGACCGAAGCGGTGCGCGCGACGCCGTCGATCCGCGTACGCGAA
GGCTGGTTCGCCGAGGCGCTGCTGACCGACGGCGACGCCGTCGCCGGGCTGCAATTGCGCCGCGCCGGCAGCGTCCTCAC
CGCACCGGTGGCGATCGCGGCGCGGGCCGTGGTGCTGGCGACCGGCGGCATCGGGCATCTCTACGCGGTTACGACCAATC
CGCCGGAAGCCTCCGGTCAGGGGCTGGCGATCGCCGCACGCGCCGGCGCCGTCATTGCCGATCCGGAATTCGTGCAGTTT
CATCCGACCGCCATCATGGTCGGCCGCGATCCGGCGCCGCTCGCCACCGAGGCGCTGCGTGGCGAGGGCGCGACGCTGAT
CAACGGCCGCGGCGAGCGCTTCATGCTGGCGTTGAATCCGCTCGCCGAGCTGGCGCCGCGCGATATCGTCGCCCGCGGCG
TGTTCGCCGAAGTCGCGGCGGGGCGCGGCGCGTTTCTCGACGCCACGCAGGCGCTCGGCGCGAAATTCGCGCACGAATTC
CCGACGGTGTACGAGAGCTGCATCTCGGCCGGCGTCGACCCCGCGACCCAGCCGATCCCGATCGCGCCGGCGGCGCACTA
CCACATGGGCGGCGTCGCGGCGGACGCACGCGGCCGCTCGTCGCTGAACGGGCTGTGGGCCGCCGGCGAAGTGTCCTGCA
CCGGCGCGCATGGCGCCAACCGGCTGGCCTCGAACTCGCTGCTGGAGGCGGTGGTGTATGCGGCGCGGATCGCCGAGGAC
ATCGCCGGCGCCGAATTGCGCGCGGCCACCGGCCGGTTCGATCCGACGACGCTTACGCGCGACGCAGCGCTCGACCCGGT
GCAGGAGCAGAGCCTGCGCGCCATCATGTCGGCCAAAGTCGGCGTGATCCGCGACCGCGATGGCCTGACCGACGCGGTGC
GGACGATCGCGGCGCTGGAAGCCGAGGCGGGCTCGACCGCGCTGACGAACATGGCGACGACCGCACTCCTGGTCGCGACC
GCGGCGCTGGCGCGGACCGAGAGCCGCGGCGCGCATTATCGCTCCGACTTTCCGGCGGAAGATCCGGCGCTGGCGGAGCG
TAGCATGACCACGCTCGCGCAGGCCCGCGAGCGCGCCGCGGCACTGAGCAAAGGCGCCAACATTCTGCGGTTTCCGACCC
GGGCCCGCACCGCGTCGGCCTGA

Upstream 100 bases:

>100_bases
GCGTCTTGGACGGCGGCCTTTCCTCCTGGCGGCCGCCGTCTCCACCTCGCAATCCGCGCACGACGCTCCGCGGACGCAGG
CTGAGGATCGGACCACCCCG

Downstream 100 bases:

>100_bases
TCCCTCCCCGTCTGCCTGCTCAGGAGCAGTTCCCATGATTGCAACGACTTTGCTGCATCCCGACGCCTTCCTGTCGCCGC
TGGCGATCGAGGAGGCCGTC

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MGLSTEIESLGAHGDIVIVGGGLAGLFCALKLAPRPVTVITAAALGEGASTAWAQGGIAAAVGEGDSAESHAADTIAVGA
GLVDEAVALGLAQEASARIHDLLAYGVPFDRDLEGRLAVAREAAHSARRIVHVRGDMAGKAIITALTEAVRATPSIRVRE
GWFAEALLTDGDAVAGLQLRRAGSVLTAPVAIAARAVVLATGGIGHLYAVTTNPPEASGQGLAIAARAGAVIADPEFVQF
HPTAIMVGRDPAPLATEALRGEGATLINGRGERFMLALNPLAELAPRDIVARGVFAEVAAGRGAFLDATQALGAKFAHEF
PTVYESCISAGVDPATQPIPIAPAAHYHMGGVAADARGRSSLNGLWAAGEVSCTGAHGANRLASNSLLEAVVYAARIAED
IAGAELRAATGRFDPTTLTRDAALDPVQEQSLRAIMSAKVGVIRDRDGLTDAVRTIAALEAEAGSTALTNMATTALLVAT
AALARTESRGAHYRSDFPAEDPALAERSMTTLAQARERAAALSKGANILRFPTRARTASA

Sequences:

>Translated_540_residues
MGLSTEIESLGAHGDIVIVGGGLAGLFCALKLAPRPVTVITAAALGEGASTAWAQGGIAAAVGEGDSAESHAADTIAVGA
GLVDEAVALGLAQEASARIHDLLAYGVPFDRDLEGRLAVAREAAHSARRIVHVRGDMAGKAIITALTEAVRATPSIRVRE
GWFAEALLTDGDAVAGLQLRRAGSVLTAPVAIAARAVVLATGGIGHLYAVTTNPPEASGQGLAIAARAGAVIADPEFVQF
HPTAIMVGRDPAPLATEALRGEGATLINGRGERFMLALNPLAELAPRDIVARGVFAEVAAGRGAFLDATQALGAKFAHEF
PTVYESCISAGVDPATQPIPIAPAAHYHMGGVAADARGRSSLNGLWAAGEVSCTGAHGANRLASNSLLEAVVYAARIAED
IAGAELRAATGRFDPTTLTRDAALDPVQEQSLRAIMSAKVGVIRDRDGLTDAVRTIAALEAEAGSTALTNMATTALLVAT
AALARTESRGAHYRSDFPAEDPALAERSMTTLAQARERAAALSKGANILRFPTRARTASA
>Mature_539_residues
GLSTEIESLGAHGDIVIVGGGLAGLFCALKLAPRPVTVITAAALGEGASTAWAQGGIAAAVGEGDSAESHAADTIAVGAG
LVDEAVALGLAQEASARIHDLLAYGVPFDRDLEGRLAVAREAAHSARRIVHVRGDMAGKAIITALTEAVRATPSIRVREG
WFAEALLTDGDAVAGLQLRRAGSVLTAPVAIAARAVVLATGGIGHLYAVTTNPPEASGQGLAIAARAGAVIADPEFVQFH
PTAIMVGRDPAPLATEALRGEGATLINGRGERFMLALNPLAELAPRDIVARGVFAEVAAGRGAFLDATQALGAKFAHEFP
TVYESCISAGVDPATQPIPIAPAAHYHMGGVAADARGRSSLNGLWAAGEVSCTGAHGANRLASNSLLEAVVYAARIAEDI
AGAELRAATGRFDPTTLTRDAALDPVQEQSLRAIMSAKVGVIRDRDGLTDAVRTIAALEAEAGSTALTNMATTALLVATA
ALARTESRGAHYRSDFPAEDPALAERSMTTLAQARERAAALSKGANILRFPTRARTASA

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=516, Percent_Identity=31.7829457364341, Blast_Score=182, Evalue=7e-46,
Organism=Escherichia coli, GI1788928, Length=521, Percent_Identity=39.3474088291747, Blast_Score=296, Evalue=3e-81,
Organism=Escherichia coli, GI1786942, Length=556, Percent_Identity=31.6546762589928, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI1790597, Length=510, Percent_Identity=32.3529411764706, Blast_Score=186, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17505833, Length=512, Percent_Identity=31.0546875, Blast_Score=182, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17550100, Length=519, Percent_Identity=30.0578034682081, Blast_Score=179, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI71986328, Length=453, Percent_Identity=29.1390728476821, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322701, Length=516, Percent_Identity=31.7829457364341, Blast_Score=200, Evalue=5e-52,
Organism=Saccharomyces cerevisiae, GI6322416, Length=517, Percent_Identity=31.3346228239845, Blast_Score=192, Evalue=2e-49,
Organism=Drosophila melanogaster, GI17137288, Length=513, Percent_Identity=31.7738791423002, Blast_Score=188, Evalue=8e-48,
Organism=Drosophila melanogaster, GI24655642, Length=513, Percent_Identity=31.7738791423002, Blast_Score=188, Evalue=8e-48,
Organism=Drosophila melanogaster, GI24655647, Length=513, Percent_Identity=31.7738791423002, Blast_Score=188, Evalue=8e-48,
Organism=Drosophila melanogaster, GI24663005, Length=528, Percent_Identity=29.1666666666667, Blast_Score=180, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 55033; Mature: 54902

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLSTEIESLGAHGDIVIVGGGLAGLFCALKLAPRPVTVITAAALGEGASTAWAQGGIAA
CCCCCCHHHCCCCCCEEEECCCHHHHHHHHHCCCCCEEEEEEEHHCCCCCCHHHCCCEEE
AVGEGDSAESHAADTIAVGAGLVDEAVALGLAQEASARIHDLLAYGVPFDRDLEGRLAVA
EECCCCCCCHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
REAAHSARRIVHVRGDMAGKAIITALTEAVRATPSIRVREGWFAEALLTDGDAVAGLQLR
HHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCEEHHHCCCCCCCCCEEHH
RAGSVLTAPVAIAARAVVLATGGIGHLYAVTTNPPEASGQGLAIAARAGAVIADPEFVQF
HCCCCHHHHHHHHHEEEEEEECCCCEEEEEECCCCCCCCCCEEEEEECCEEEECCCEEEE
HPTAIMVGRDPAPLATEALRGEGATLINGRGERFMLALNPLAELAPRDIVARGVFAEVAA
ECCEEEECCCCCCHHHHHHCCCCCEEECCCCCEEEEEECHHHHHCCHHHHHHHHHHHHHC
GRGAFLDATQALGAKFAHEFPTVYESCISAGVDPATQPIPIAPAAHYHMGGVAADARGRS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCC
SLNGLWAAGEVSCTGAHGANRLASNSLLEAVVYAARIAEDIAGAELRAATGRFDPTTLTR
CCCCEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCC
DAALDPVQEQSLRAIMSAKVGVIRDRDGLTDAVRTIAALEAEAGSTALTNMATTALLVAT
CCCCCCHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
AALARTESRGAHYRSDFPAEDPALAERSMTTLAQARERAAALSKGANILRFPTRARTASA
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCC
>Mature Secondary Structure 
GLSTEIESLGAHGDIVIVGGGLAGLFCALKLAPRPVTVITAAALGEGASTAWAQGGIAA
CCCCCHHHCCCCCCEEEECCCHHHHHHHHHCCCCCEEEEEEEHHCCCCCCHHHCCCEEE
AVGEGDSAESHAADTIAVGAGLVDEAVALGLAQEASARIHDLLAYGVPFDRDLEGRLAVA
EECCCCCCCHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
REAAHSARRIVHVRGDMAGKAIITALTEAVRATPSIRVREGWFAEALLTDGDAVAGLQLR
HHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCEEHHHCCCCCCCCCEEHH
RAGSVLTAPVAIAARAVVLATGGIGHLYAVTTNPPEASGQGLAIAARAGAVIADPEFVQF
HCCCCHHHHHHHHHEEEEEEECCCCEEEEEECCCCCCCCCCEEEEEECCEEEECCCEEEE
HPTAIMVGRDPAPLATEALRGEGATLINGRGERFMLALNPLAELAPRDIVARGVFAEVAA
ECCEEEECCCCCCHHHHHHCCCCCEEECCCCCEEEEEECHHHHHCCHHHHHHHHHHHHHC
GRGAFLDATQALGAKFAHEFPTVYESCISAGVDPATQPIPIAPAAHYHMGGVAADARGRS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCC
SLNGLWAAGEVSCTGAHGANRLASNSLLEAVVYAARIAEDIAGAELRAATGRFDPTTLTR
CCCCEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCC
DAALDPVQEQSLRAIMSAKVGVIRDRDGLTDAVRTIAALEAEAGSTALTNMATTALLVAT
CCCCCCHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
AALARTESRGAHYRSDFPAEDPALAERSMTTLAQARERAAALSKGANILRFPTRARTASA
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]