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The map label for this gene is ligB [H]

Identifier: 86748186

GI number: 86748186

Start: 1215467

End: 1216324

Strand: Direct

Name: ligB [H]

Synonym: RPB_1061

Alternate gene names: 86748186

Gene position: 1215467-1216324 (Clockwise)

Preceding gene: 86748185

Following gene: 86748187

Centisome position: 22.8

GC content: 66.55

Gene sequence:

>858_bases
ATGGCGACGATCATCGGCGGCCTCGCCGCCTCCCACGTGCCCGCGATCGGCGGCGCGATCGCCAAGGGCCTGCAGAAGGA
CCCGTATTGGGCGCCGTTCTTCGACGGCTTCGACGCCACGCATCGCTGGCTCGACGCGGTGAAGCCCGACGCCGCGGTGG
TGATCTACAACGATCACGGCCTCAACTTCTTCCTCGACAAGATGCCGACCTTCGCGGTCGGCGCGGCGCCGGAGTATCGC
AACGACGACGAGGGCTGGGGCATCCCGGTGCGCGCGCCGTTCCGCGGCGACACCGCGCTGTCGTGGCACATCATCGAGAC
GCTGGTCGGCGCCGAATTCGATCCGGTCACCTGCCAGGAGATGCTGGTCGACCACGCCTACAGCCTGCCGATGGAACTGT
TCTTTCGCGAAAGGGCGCACGACATCCCGACCGTGCCGGTGGTCGTCAACACCGTGCAGCATCCGCTGCCGTCGCTGCGC
CGCTGCTTCAAGCTCGGCCAGGCGATCGGCCGCGCCATCGAATCCTATCCTGAAGATCTGAAAGTCGTGGTGATCGGCAC
CGGCGGGCTGTCGCACCAGCTCGAAGGCGAGCGCGCCGGCTTCATCAACACCGCCTTCGACCGCCGCTGCATGGACGCGA
TGACCGGCGATATCGACAGCCTCTTGACGATCGACCCGCACGAGCTGGTCGAACTCGCCGGCAGCCAGGGCGTCGAAGTG
ATGAACTGGGTGGCAATGCGCGGCGCGCTCACCGGCGACGTCCACGAGCTGCACCGCAACTATCATCTGCCGATCTCCAA
CACCGCGTCGGGAATCATGCTGCTGGAGAATAGGCCGAAGCTGGCGCAGGCGGCGTAG

Upstream 100 bases:

>100_bases
TCAACATGCAGGACATCGGGGCGCTGCAGACCGGCATGAGCGTCGAGCAGTTCAAGGCCAAGCTTTTGGCCGCCAGAGAT
AGTTGAAGGGGAGAGCCGAG

Downstream 100 bases:

>100_bases
CGCTTCGTCGGTTCGCCGCACTCGCCCAACCAACCACCGTCATGCCCGGGCTTGACCCGGGCATCCACGAAGCCAAGCAG
CGCGGTGCCTGTTGACGTCG

Product: protocatechuate 4,5-dioxygenase subunit beta

Products: NA

Alternate protein names: 4,5-PCD [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MATIIGGLAASHVPAIGGAIAKGLQKDPYWAPFFDGFDATHRWLDAVKPDAAVVIYNDHGLNFFLDKMPTFAVGAAPEYR
NDDEGWGIPVRAPFRGDTALSWHIIETLVGAEFDPVTCQEMLVDHAYSLPMELFFRERAHDIPTVPVVVNTVQHPLPSLR
RCFKLGQAIGRAIESYPEDLKVVVIGTGGLSHQLEGERAGFINTAFDRRCMDAMTGDIDSLLTIDPHELVELAGSQGVEV
MNWVAMRGALTGDVHELHRNYHLPISNTASGIMLLENRPKLAQAA

Sequences:

>Translated_285_residues
MATIIGGLAASHVPAIGGAIAKGLQKDPYWAPFFDGFDATHRWLDAVKPDAAVVIYNDHGLNFFLDKMPTFAVGAAPEYR
NDDEGWGIPVRAPFRGDTALSWHIIETLVGAEFDPVTCQEMLVDHAYSLPMELFFRERAHDIPTVPVVVNTVQHPLPSLR
RCFKLGQAIGRAIESYPEDLKVVVIGTGGLSHQLEGERAGFINTAFDRRCMDAMTGDIDSLLTIDPHELVELAGSQGVEV
MNWVAMRGALTGDVHELHRNYHLPISNTASGIMLLENRPKLAQAA
>Mature_284_residues
ATIIGGLAASHVPAIGGAIAKGLQKDPYWAPFFDGFDATHRWLDAVKPDAAVVIYNDHGLNFFLDKMPTFAVGAAPEYRN
DDEGWGIPVRAPFRGDTALSWHIIETLVGAEFDPVTCQEMLVDHAYSLPMELFFRERAHDIPTVPVVVNTVQHPLPSLRR
CFKLGQAIGRAIESYPEDLKVVVIGTGGLSHQLEGERAGFINTAFDRRCMDAMTGDIDSLLTIDPHELVELAGSQGVEVM
NWVAMRGALTGDVHELHRNYHLPISNTASGIMLLENRPKLAQAA

Specific function: Responsible for the aromatic ring fission of protocatechuate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786544, Length=150, Percent_Identity=31.3333333333333, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004183 [H]

Pfam domain/function: PF02900 LigB [H]

EC number: =1.13.11.8 [H]

Molecular weight: Translated: 31220; Mature: 31089

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIIGGLAASHVPAIGGAIAKGLQKDPYWAPFFDGFDATHRWLDAVKPDAAVVIYNDHG
CCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCC
LNFFLDKMPTFAVGAAPEYRNDDEGWGIPVRAPFRGDTALSWHIIETLVGAEFDPVTCQE
CEEHHHCCCCEEECCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHH
MLVDHAYSLPMELFFRERAHDIPTVPVVVNTVQHPLPSLRRCFKLGQAIGRAIESYPEDL
HHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCE
KVVVIGTGGLSHQLEGERAGFINTAFDRRCMDAMTGDIDSLLTIDPHELVELAGSQGVEV
EEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHEEEECHHHHHHHHCCCCHHH
MNWVAMRGALTGDVHELHRNYHLPISNTASGIMLLENRPKLAQAA
HHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCEEEECCCCCHHCCC
>Mature Secondary Structure 
ATIIGGLAASHVPAIGGAIAKGLQKDPYWAPFFDGFDATHRWLDAVKPDAAVVIYNDHG
CEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCC
LNFFLDKMPTFAVGAAPEYRNDDEGWGIPVRAPFRGDTALSWHIIETLVGAEFDPVTCQE
CEEHHHCCCCEEECCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHH
MLVDHAYSLPMELFFRERAHDIPTVPVVVNTVQHPLPSLRRCFKLGQAIGRAIESYPEDL
HHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCE
KVVVIGTGGLSHQLEGERAGFINTAFDRRCMDAMTGDIDSLLTIDPHELVELAGSQGVEV
EEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHEEEECHHHHHHHHCCCCHHH
MNWVAMRGALTGDVHELHRNYHLPISNTASGIMLLENRPKLAQAA
HHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCEEEECCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185230 [H]