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The map label for this gene is ligA [H]

Identifier: 86748185

GI number: 86748185

Start: 1215069

End: 1215452

Strand: Direct

Name: ligA [H]

Synonym: RPB_1060

Alternate gene names: 86748185

Gene position: 1215069-1215452 (Clockwise)

Preceding gene: 86748184

Following gene: 86748186

Centisome position: 22.79

GC content: 63.02

Gene sequence:

>384_bases
ATGAGCCAGGCTTTGGCCAAACAGCCAGAAGCAGGCGCCTATTTGAAGCGCGACATCTTCGGCACGCCGATCTTCGGCCC
GGAAGCGGCGCAGAAGGGCTACGCTCTCAACAAGATGTGCTTCTCCTTCAATTCCGCCGCCGCCCGCGCCGACTTCGTCG
CGGACGAGGCGGCCTATTGCGCGCGCTACGGCCTCACCGCCACGCAGACCGAGGCGATCCTGAAGCGCGATCTGCTGAAG
CTGCTCGATCACGGCGGCAACGCCTACTACCTCGCCAAATTCGCCGGCATCCTCGGCCTCAACATGCAGGACATCGGGGC
GCTGCAGACCGGCATGAGCGTCGAGCAGTTCAAGGCCAAGCTTTTGGCCGCCAGAGATAGTTGA

Upstream 100 bases:

>100_bases
GTCGATTTGATCCAGCTCAATTCCGGGTTCCCGCCCCCGCCTATCGTTCGCGAAACGCGGCGCCACGGCCGCAGGCGGAA
ACGATCGGGAGGGGACGGCG

Downstream 100 bases:

>100_bases
AGGGGAGAGCCGAGATGGCGACGATCATCGGCGGCCTCGCCGCCTCCCACGTGCCCGCGATCGGCGGCGCGATCGCCAAG
GGCCTGCAGAAGGACCCGTA

Product: protocatechuate 4,5-dioxygenase subunit alpha

Products: NA

Alternate protein names: 4,5-PCD [H]

Number of amino acids: Translated: 127; Mature: 126

Protein sequence:

>127_residues
MSQALAKQPEAGAYLKRDIFGTPIFGPEAAQKGYALNKMCFSFNSAAARADFVADEAAYCARYGLTATQTEAILKRDLLK
LLDHGGNAYYLAKFAGILGLNMQDIGALQTGMSVEQFKAKLLAARDS

Sequences:

>Translated_127_residues
MSQALAKQPEAGAYLKRDIFGTPIFGPEAAQKGYALNKMCFSFNSAAARADFVADEAAYCARYGLTATQTEAILKRDLLK
LLDHGGNAYYLAKFAGILGLNMQDIGALQTGMSVEQFKAKLLAARDS
>Mature_126_residues
SQALAKQPEAGAYLKRDIFGTPIFGPEAAQKGYALNKMCFSFNSAAARADFVADEAAYCARYGLTATQTEAILKRDLLKL
LDHGGNAYYLAKFAGILGLNMQDIGALQTGMSVEQFKAKLLAARDS

Specific function: Responsible for the aromatic ring fission of protocatechuate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014159
- InterPro:   IPR011986 [H]

Pfam domain/function: PF07746 LigA [H]

EC number: =1.13.11.8 [H]

Molecular weight: Translated: 13662; Mature: 13531

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQALAKQPEAGAYLKRDIFGTPIFGPEAAQKGYALNKMCFSFNSAAARADFVADEAAYC
CCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHH
ARYGLTATQTEAILKRDLLKLLDHGGNAYYLAKFAGILGLNMQDIGALQTGMSVEQFKAK
HHHCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCHHHHHHHHHCCCHHHHHHH
LLAARDS
HHHHCCC
>Mature Secondary Structure 
SQALAKQPEAGAYLKRDIFGTPIFGPEAAQKGYALNKMCFSFNSAAARADFVADEAAYC
CCCCCCCCCCCCHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHH
ARYGLTATQTEAILKRDLLKLLDHGGNAYYLAKFAGILGLNMQDIGALQTGMSVEQFKAK
HHHCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCHHHHHHHHHCCCHHHHHHH
LLAARDS
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185230 [H]