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The map label for this gene is rutB [H]

Identifier: 86748142

GI number: 86748142

Start: 1165747

End: 1166448

Strand: Direct

Name: rutB [H]

Synonym: RPB_1017

Alternate gene names: 86748142

Gene position: 1165747-1166448 (Clockwise)

Preceding gene: 86748141

Following gene: 86748143

Centisome position: 21.86

GC content: 68.23

Gene sequence:

>702_bases
ATGGCCGCCACGACTTTCTCAGCCTCGGGCACGATCGACGCCGAGCCGGCGCCGATCGCGCTCGACTGGGTCGCGACCGC
GCTCTTGATCATCGACATGCAGCGCGACTTCCTGGAGCCGGGCGGCTTCGGCGAAACGCTGGGCAACGACGTCTCGCGGC
TCGGCCGCGCGGTCGGGCCGATCGCCGCGGTGCTGGCCGCGGCGCGGGCGATGGGTCTGCTGGTGGTGCACACCCGCGAG
GGCCATCTGCCGGATCTGACCGATGCGCCACCGGCCAAACTCGCACGCGGCGCGCCGTCGCTGCGGATCGGCGATCCCGG
GCCGATGGGCCGCATCCTGATCCGCGGCGAGCCGGGGCACGACATCATCCCGGAGTTGTATCCGCGCGACGATGAAATCG
TCATCGACAAGCCCGGCAAGGGCGCGTTCTTCGCCACCGAACTCGACGACGTGCTGCAGAAATACGGCATCGAGAATCTG
CTGGTCTGTGGCGTCACCACCGAAGTCTGCGTCAACACCACGGTGCGCGAGGCCAATGATCGCGGCTATCGCTGCGTGGT
GATCGGCGACGGCTGCGCGTCGTATTTTCCGGACTTCCATGATGCCGGGCTCGCGATGATCAAGGCGCAGGGCGGCATCT
TCGGCTGGGTTGCGGACTCCGCCGCGGTACTCGCCGCGATGGCGCCGATCGTGGACGATTGA

Upstream 100 bases:

>100_bases
GTCGGCACGCTGGTGGCGCTGCTGATCGGCTGAACGGCGCTTTCATCCGTCATGTCTGGCGTTCTATGCTGCGATCGATT
CCGATCGCGGAGCAACGACG

Downstream 100 bases:

>100_bases
GCGATCACCGGCAGTTGACGAAACCGGAACGGTCCTGCGCGGTTGTTTCCTTCGGCGTTCGCACTGAAAGGAACCAACAT
ATGAGAACTCATTCCGCTGC

Product: isochorismatase hydrolase

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 233; Mature: 232

Protein sequence:

>233_residues
MAATTFSASGTIDAEPAPIALDWVATALLIIDMQRDFLEPGGFGETLGNDVSRLGRAVGPIAAVLAAARAMGLLVVHTRE
GHLPDLTDAPPAKLARGAPSLRIGDPGPMGRILIRGEPGHDIIPELYPRDDEIVIDKPGKGAFFATELDDVLQKYGIENL
LVCGVTTEVCVNTTVREANDRGYRCVVIGDGCASYFPDFHDAGLAMIKAQGGIFGWVADSAAVLAAMAPIVDD

Sequences:

>Translated_233_residues
MAATTFSASGTIDAEPAPIALDWVATALLIIDMQRDFLEPGGFGETLGNDVSRLGRAVGPIAAVLAAARAMGLLVVHTRE
GHLPDLTDAPPAKLARGAPSLRIGDPGPMGRILIRGEPGHDIIPELYPRDDEIVIDKPGKGAFFATELDDVLQKYGIENL
LVCGVTTEVCVNTTVREANDRGYRCVVIGDGCASYFPDFHDAGLAMIKAQGGIFGWVADSAAVLAAMAPIVDD
>Mature_232_residues
AATTFSASGTIDAEPAPIALDWVATALLIIDMQRDFLEPGGFGETLGNDVSRLGRAVGPIAAVLAAARAMGLLVVHTREG
HLPDLTDAPPAKLARGAPSLRIGDPGPMGRILIRGEPGHDIIPELYPRDDEIVIDKPGKGAFFATELDDVLQKYGIENLL
VCGVTTEVCVNTTVREANDRGYRCVVIGDGCASYFPDFHDAGLAMIKAQGGIFGWVADSAAVLAAMAPIVDD

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=225, Percent_Identity=31.5555555555556, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI87081992, Length=190, Percent_Identity=28.4210526315789, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 24450; Mature: 24319

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATTFSASGTIDAEPAPIALDWVATALLIIDMQRDFLEPGGFGETLGNDVSRLGRAVGP
CCCEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
IAAVLAAARAMGLLVVHTREGHLPDLTDAPPAKLARGAPSLRIGDPGPMGRILIRGEPGH
HHHHHHHHHHHCEEEEEECCCCCCCCCCCCHHHHHCCCCCEEECCCCCCCEEEEECCCCC
DIIPELYPRDDEIVIDKPGKGAFFATELDDVLQKYGIENLLVCGVTTEVCVNTTVREAND
CCCCCCCCCCCCEEEECCCCCCEEEHHHHHHHHHCCCCEEEEECCHHHHHHCCHHHCCCC
RGYRCVVIGDGCASYFPDFHDAGLAMIKAQGGIFGWVADSAAVLAAMAPIVDD
CCCEEEEEECCHHHHCCCCCCCCEEEEEECCCEEEEHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AATTFSASGTIDAEPAPIALDWVATALLIIDMQRDFLEPGGFGETLGNDVSRLGRAVGP
CCEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
IAAVLAAARAMGLLVVHTREGHLPDLTDAPPAKLARGAPSLRIGDPGPMGRILIRGEPGH
HHHHHHHHHHHCEEEEEECCCCCCCCCCCCHHHHHCCCCCEEECCCCCCCEEEEECCCCC
DIIPELYPRDDEIVIDKPGKGAFFATELDDVLQKYGIENLLVCGVTTEVCVNTTVREAND
CCCCCCCCCCCCEEEECCCCCCEEEHHHHHHHHHCCCCEEEEECCHHHHHHCCHHHCCCC
RGYRCVVIGDGCASYFPDFHDAGLAMIKAQGGIFGWVADSAAVLAAMAPIVDD
CCCEEEEEECCHHHHCCCCCCCCEEEEEECCCEEEEHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA