Click here to switch to the map view.

The map label for this gene is 86748118

Identifier: 86748118

GI number: 86748118

Start: 1136935

End: 1137876

Strand: Direct

Name: 86748118

Synonym: RPB_0993

Alternate gene names: NA

Gene position: 1136935-1137876 (Clockwise)

Preceding gene: 86748117

Following gene: 86748122

Centisome position: 21.32

GC content: 68.05

Gene sequence:

>942_bases
ATGAACGTCGCGATCCGCAAGGTTCCGTCCGAAGCCCACGCGCAGCCGGTCGATGCGCTCGATCATCTCGCCGAGCAGCT
GTTTCATCGCATGGGCGCCGACGGCGTCTATGCCCGCACCGCGCTGTACGAGGGCGTGGTCGAGAAACTCGCGGCGCTGA
TCTCGCGGCATCGCGAGGCCGGCACCGAAGTGATGCGGTTTCCGCCGGTGATGAGCCGCGCCCAGCTGGAGAAATCCGGC
TATCTGAAGAGCTTCCCGAATCTGCTCGGCTGCGTCTGCGGCCTGCACGGCACCGAGCAGGAGATCAATGCGGCGGTGAG
CCGGTTCGACGCCGGCGGCGACTGGACGAGTTCGCTGTCGCCGGCCGATCTGGTGCTGTCGCCGGCGGCCTGCTATCCGG
TCTATCCGCTCGCCGCGAGCCGCGGTCCGTTGCCGGCCGGCGGGCTGCGCTTCGACGTCGCCGCCGATTGCTTCCGCCGC
GAGCCGTCGAAACATCTCGACCGGCTGCAATCGTTCCGGATGCGCGAATATGTCTGCATCGGCACGTCGCAGGACGTCGC
CGATTTCCGCGAGCGCTGGATGGGCAAGGCGCAGGCGATCGCCCGCGATCTCGGCCTCGCCTTCCGGGTCGACACCGCGT
CGGATCCGTTCTTCGGCCGCGTCGGCCAGATGAAGGCGATCAGCCAGCAGCAGCAGGCGCTGAAATTCGAACTGCTGGTG
CCGCTGCGCTCGGAAGAGCAGCCGACCGCCTGCATGAGCTTCAACTATCACCGCGAACATTTCGGCGTCACCTGGGGCAT
CGAGGATGTCGCCGGCCAGCCGGCGCACACCGGCTGCGTCGCCTTCGGCATGGACCGCCTCGCGGTGGCGCTGTTTCACA
CCCACGGCATCGACACCGCCGCCTGGCCGGCGCGCGTGCGGGAGATGCTGGCGCTGGATTAG

Upstream 100 bases:

>100_bases
GCATCCTCGCCAATATCGCCACCGCCAACCTGATGAGCGCGGTGCCGGCGAGCCTGCGCGACTGAACGCACCGTCACCGA
TCTTTCGAGGAGGCCACCTC

Downstream 100 bases:

>100_bases
CCACCTCTCCCGCTTGCGGGAGAGGTCGGATCGCGTCAGCGATCCGGGTGAGGGCACTCCTCTCCGCGATCGCGGGACTC
ACTGCCTGGGGCGCCCTCAC

Product: hypothetical protein

Products: AMP; diphosphate; L-seryl-tRNA(Ser)

Alternate protein names: Amino acid:[carrier-protein] ligase [AMP forming] 1; aa:CP ligase 1; Aminoacyl-[acyl-carrier-protein] synthetase 1; L-glycine:[acyl-carrier-protein] ligase 1 [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNVAIRKVPSEAHAQPVDALDHLAEQLFHRMGADGVYARTALYEGVVEKLAALISRHREAGTEVMRFPPVMSRAQLEKSG
YLKSFPNLLGCVCGLHGTEQEINAAVSRFDAGGDWTSSLSPADLVLSPAACYPVYPLAASRGPLPAGGLRFDVAADCFRR
EPSKHLDRLQSFRMREYVCIGTSQDVADFRERWMGKAQAIARDLGLAFRVDTASDPFFGRVGQMKAISQQQQALKFELLV
PLRSEEQPTACMSFNYHREHFGVTWGIEDVAGQPAHTGCVAFGMDRLAVALFHTHGIDTAAWPARVREMLALD

Sequences:

>Translated_313_residues
MNVAIRKVPSEAHAQPVDALDHLAEQLFHRMGADGVYARTALYEGVVEKLAALISRHREAGTEVMRFPPVMSRAQLEKSG
YLKSFPNLLGCVCGLHGTEQEINAAVSRFDAGGDWTSSLSPADLVLSPAACYPVYPLAASRGPLPAGGLRFDVAADCFRR
EPSKHLDRLQSFRMREYVCIGTSQDVADFRERWMGKAQAIARDLGLAFRVDTASDPFFGRVGQMKAISQQQQALKFELLV
PLRSEEQPTACMSFNYHREHFGVTWGIEDVAGQPAHTGCVAFGMDRLAVALFHTHGIDTAAWPARVREMLALD
>Mature_313_residues
MNVAIRKVPSEAHAQPVDALDHLAEQLFHRMGADGVYARTALYEGVVEKLAALISRHREAGTEVMRFPPVMSRAQLEKSG
YLKSFPNLLGCVCGLHGTEQEINAAVSRFDAGGDWTSSLSPADLVLSPAACYPVYPLAASRGPLPAGGLRFDVAADCFRR
EPSKHLDRLQSFRMREYVCIGTSQDVADFRERWMGKAQAIARDLGLAFRVDTASDPFFGRVGQMKAISQQQQALKFELLV
PLRSEEQPTACMSFNYHREHFGVTWGIEDVAGQPAHTGCVAFGMDRLAVALFHTHGIDTAAWPARVREMLALD

Specific function: Catalyzes the ATP-dependent activation of L-glycine and its transfer to the phosphopantetheine prosthetic group covalently attached to the vicinal carrier protein bsr0959 of yet unknown function. May participate in nonribosomal peptide synthesis or relate

COG id: COG0172

COG function: function code J; Seryl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Amino acid--[acyl-carrier-protein] ligase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314 [H]

Pfam domain/function: PF00587 tRNA-synt_2b [H]

EC number: 6.1.1.11

Molecular weight: Translated: 34460; Mature: 34460

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVAIRKVPSEAHAQPVDALDHLAEQLFHRMGADGVYARTALYEGVVEKLAALISRHREA
CCCCEECCCCHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GTEVMRFPPVMSRAQLEKSGYLKSFPNLLGCVCGLHGTEQEINAAVSRFDAGGDWTSSLS
CCHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCC
PADLVLSPAACYPVYPLAASRGPLPAGGLRFDVAADCFRREPSKHLDRLQSFRMREYVCI
CHHHEECCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHEEEE
GTSQDVADFRERWMGKAQAIARDLGLAFRVDTASDPFFGRVGQMKAISQQQQALKFELLV
CCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHEEE
PLRSEEQPTACMSFNYHREHFGVTWGIEDVAGQPAHTGCVAFGMDRLAVALFHTHGIDTA
CCCCCCCCCCHHCCCCHHHHCCEEECHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCH
AWPARVREMLALD
HHHHHHHHHHHCC
>Mature Secondary Structure
MNVAIRKVPSEAHAQPVDALDHLAEQLFHRMGADGVYARTALYEGVVEKLAALISRHREA
CCCCEECCCCHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GTEVMRFPPVMSRAQLEKSGYLKSFPNLLGCVCGLHGTEQEINAAVSRFDAGGDWTSSLS
CCHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCC
PADLVLSPAACYPVYPLAASRGPLPAGGLRFDVAADCFRREPSKHLDRLQSFRMREYVCI
CHHHEECCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHEEEE
GTSQDVADFRERWMGKAQAIARDLGLAFRVDTASDPFFGRVGQMKAISQQQQALKFELLV
CCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHEEE
PLRSEEQPTACMSFNYHREHFGVTWGIEDVAGQPAHTGCVAFGMDRLAVALFHTHGIDTA
CCCCCCCCCCHHCCCCHHHHCCEEECHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCH
AWPARVREMLALD
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-serine; tRNA(Ser)

Specific reaction: ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser)

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]