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The map label for this gene is nifV [H]

Identifier: 86748107

GI number: 86748107

Start: 1127421

End: 1128590

Strand: Direct

Name: nifV [H]

Synonym: RPB_0982

Alternate gene names: 86748107

Gene position: 1127421-1128590 (Clockwise)

Preceding gene: 86748106

Following gene: 86748108

Centisome position: 21.15

GC content: 69.23

Gene sequence:

>1170_bases
ATGCGTGACAGCAGCGCAACGCCATCGACCGCGACGCCGGTCGTGCTCAACGACACCACGCTGCGCGACGGCGAGCAGGC
GCCGGGCGTGGCGTTCTCGGCCGCCGAAAAACTCGCGATCGCCCGCGCGCTGGCGCAGGCCGGCGTGCCGGAAATCGAGG
CCGGAACGCCGGCGATGGGGCCGGACGAGATCGCGGCGATCCGCAGCATCGTCGAGGCCGGGCTGCCGCTGACGACGATC
GCCTGGTGCCGGATGCGCACCGAGGATGTCGAGGCGGCGCTGCAGGCCGGCGTGGCGATGGTCAACGTTTCGGTGCCGGT
CTCCGAGGTGCAGATCGCCGCCAAGCTCGGCGGCCAGCGATCGAACGCGATCGAGACGGTGAAGCGCGTGGTCGGCTATG
CGCGCGACCACGGGCTCGAGGTCGCGGTCGGCGGCGAGGATTCGTCGCGCGCCGATCCGGAGTTCCTGGCCGAAGTGATC
GCCACCGCGAAGGCGGCGGGCGCGCGGCGGTTCCGGATCGCCGATACGCTCAGCGTGCTCGATCCGTTTCGTACTCACGA
ACTGATGGCGACCCTGCGCGCCACCACCGATCTCGAACTGGAATTTCACGGCCACGACGATCTCGGCCTCGCCACCGCCA
ACACCCTGGCGGCGCTGCAGGCCGGCGCCAGCCATGCGTCGGTCACGGTGATCGGGCTGGGCGAGCGCGCCGGCAACGCG
CCGCTGGAGGAGGTGGCCGTCGCGCTGAAGCAGCTTTACGGCCGCGATACCGGCATCGTGCTGTCCGAACTCGGCCATGT
CGCCGATGTGGTGGCGGCCGCCGCCGCGCGGACGATTCCGCTCAACAAGGCGATCGTCGGCGAACACGTCTTCACCCACG
AATCCGGCATTCATGTCGACGGCCTGCTCAAGGACCAGCGCACCTATCAGTCGCTCGATCCGGTGCTGCTCGGCCGCGCC
AATTGCATCGTGATCGGCAAGCATTCCGGCCTGTCGGCGATCACCTCGTCGCTGGCCAAGCTCAATCTGCCGGCGACGGC
CGAAGAGGCGCAGGGCATTCTGGCGCAAGTGCGCCTGCATGCGGTCGCCAACAAGGGCCCGGTCGGAAACGAGACATTGA
CGGCGATCTGGCGTGAGGTTCACGACCGCACGCTGCCGAACTGCGCCTGA

Upstream 100 bases:

>100_bases
GACATCGAGCGCGCGCTCGCCGTCATCCCCGGCGTGATCGCCAAGCTGCGCGCGATCTCGCCGTTCACCGCCGATGACGG
ACCTTTGCTCGGGCGCGCCC

Downstream 100 bases:

>100_bases
GCGCAACGTTGCGCGGCGAACGAGAGATGAAGGAGATCGAGATGAGCGCGACCCAGGCGAGTTCCCAACAAGCCGACAGC
AAAGCGCCGGCCGACATCAT

Product: pyruvate carboxyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MRDSSATPSTATPVVLNDTTLRDGEQAPGVAFSAAEKLAIARALAQAGVPEIEAGTPAMGPDEIAAIRSIVEAGLPLTTI
AWCRMRTEDVEAALQAGVAMVNVSVPVSEVQIAAKLGGQRSNAIETVKRVVGYARDHGLEVAVGGEDSSRADPEFLAEVI
ATAKAAGARRFRIADTLSVLDPFRTHELMATLRATTDLELEFHGHDDLGLATANTLAALQAGASHASVTVIGLGERAGNA
PLEEVAVALKQLYGRDTGIVLSELGHVADVVAAAAARTIPLNKAIVGEHVFTHESGIHVDGLLKDQRTYQSLDPVLLGRA
NCIVIGKHSGLSAITSSLAKLNLPATAEEAQGILAQVRLHAVANKGPVGNETLTAIWREVHDRTLPNCA

Sequences:

>Translated_389_residues
MRDSSATPSTATPVVLNDTTLRDGEQAPGVAFSAAEKLAIARALAQAGVPEIEAGTPAMGPDEIAAIRSIVEAGLPLTTI
AWCRMRTEDVEAALQAGVAMVNVSVPVSEVQIAAKLGGQRSNAIETVKRVVGYARDHGLEVAVGGEDSSRADPEFLAEVI
ATAKAAGARRFRIADTLSVLDPFRTHELMATLRATTDLELEFHGHDDLGLATANTLAALQAGASHASVTVIGLGERAGNA
PLEEVAVALKQLYGRDTGIVLSELGHVADVVAAAAARTIPLNKAIVGEHVFTHESGIHVDGLLKDQRTYQSLDPVLLGRA
NCIVIGKHSGLSAITSSLAKLNLPATAEEAQGILAQVRLHAVANKGPVGNETLTAIWREVHDRTLPNCA
>Mature_389_residues
MRDSSATPSTATPVVLNDTTLRDGEQAPGVAFSAAEKLAIARALAQAGVPEIEAGTPAMGPDEIAAIRSIVEAGLPLTTI
AWCRMRTEDVEAALQAGVAMVNVSVPVSEVQIAAKLGGQRSNAIETVKRVVGYARDHGLEVAVGGEDSSRADPEFLAEVI
ATAKAAGARRFRIADTLSVLDPFRTHELMATLRATTDLELEFHGHDDLGLATANTLAALQAGASHASVTVIGLGERAGNA
PLEEVAVALKQLYGRDTGIVLSELGHVADVVAAAAARTIPLNKAIVGEHVFTHESGIHVDGLLKDQRTYQSLDPVLLGRA
NCIVIGKHSGLSAITSSLAKLNLPATAEEAQGILAQVRLHAVANKGPVGNETLTAIWREVHDRTLPNCA

Specific function: This protein is a Fe-Mo-cofactor biosynthetic component [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=316, Percent_Identity=35.126582278481, Blast_Score=160, Evalue=9e-41,
Organism=Saccharomyces cerevisiae, GI6320071, Length=398, Percent_Identity=29.3969849246231, Blast_Score=171, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6320019, Length=359, Percent_Identity=30.6406685236769, Blast_Score=167, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24582381, Length=296, Percent_Identity=26.3513513513513, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.14 [H]

Molecular weight: Translated: 40516; Mature: 40516

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDSSATPSTATPVVLNDTTLRDGEQAPGVAFSAAEKLAIARALAQAGVPEIEAGTPAMG
CCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PDEIAAIRSIVEAGLPLTTIAWCRMRTEDVEAALQAGVAMVNVSVPVSEVQIAAKLGGQR
HHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCC
SNAIETVKRVVGYARDHGLEVAVGGEDSSRADPEFLAEVIATAKAAGARRFRIADTLSVL
HHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHH
DPFRTHELMATLRATTDLELEFHGHDDLGLATANTLAALQAGASHASVTVIGLGERAGNA
CHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCC
PLEEVAVALKQLYGRDTGIVLSELGHVADVVAAAAARTIPLNKAIVGEHVFTHESGIHVD
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHEECCCCCEEH
GLLKDQRTYQSLDPVLLGRANCIVIGKHSGLSAITSSLAKLNLPATAEEAQGILAQVRLH
HHHHHHHHHHHCCHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AVANKGPVGNETLTAIWREVHDRTLPNCA
HHHCCCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRDSSATPSTATPVVLNDTTLRDGEQAPGVAFSAAEKLAIARALAQAGVPEIEAGTPAMG
CCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PDEIAAIRSIVEAGLPLTTIAWCRMRTEDVEAALQAGVAMVNVSVPVSEVQIAAKLGGQR
HHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCC
SNAIETVKRVVGYARDHGLEVAVGGEDSSRADPEFLAEVIATAKAAGARRFRIADTLSVL
HHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHH
DPFRTHELMATLRATTDLELEFHGHDDLGLATANTLAALQAGASHASVTVIGLGERAGNA
CHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCC
PLEEVAVALKQLYGRDTGIVLSELGHVADVVAAAAARTIPLNKAIVGEHVFTHESGIHVD
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHEECCCCCEEH
GLLKDQRTYQSLDPVLLGRANCIVIGKHSGLSAITSSLAKLNLPATAEEAQGILAQVRLH
HHHHHHHHHHHCCHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AVANKGPVGNETLTAIWREVHDRTLPNCA
HHHCCCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644218; 3040672 [H]