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The map label for this gene is cfxB [H]

Identifier: 86748075

GI number: 86748075

Start: 1098987

End: 1100072

Strand: Direct

Name: cfxB [H]

Synonym: RPB_0950

Alternate gene names: 86748075

Gene position: 1098987-1100072 (Clockwise)

Preceding gene: 86748074

Following gene: 86748076

Centisome position: 20.61

GC content: 65.1

Gene sequence:

>1086_bases
ATGGCGCGTATCACCTTGAGGCAATTGCTGGACCACGCCGCCGAGCACGGCTATGGCGTGCCCGCGTTCAACATCAACAA
CATGGAGCAGGGACTGGCGATCATGGAGGCGGCGGCGGCGGTCGACGCGCCGGTGATCATCCAGGCCTCGCGCGGCGCGC
GCTCCTACGCCAACGACATCATGCTGGCGAAGATGATCGACGCGCTGGCGGAGATGTATCCCGACATTCCGCTGTGCATG
CATCAGGATCACGGCAACGACGAAGCCACCTGCGCCACCGCGATCAAATACGGCTTCACCTCGGTGATGATGGACGGCTC
GCTCAAGGCGGACGCCAAGACCGCGGCGGATTATGAGTACAACGTCGACATCACCCGCCGCGTCACCGACATGGCGCATT
GGGTCGGCGCCTCGGTCGAGGGCGAGCTCGGCGTGCTCGGCTCGCTCGAGCACGGCGGCGGCGAGCAGGAAGACGGTCAC
GGCGTCGAGGGCAAGGTCAGCCGCGAGCAGTTGCTGACCGATCCGGACCAGGCGGTCGATTTCGTCCGCGCCACCAAGGT
CGATGCGCTGGCGATCGCGATGGGCACCTCGCATGGCGCCTACAAATTCTCGCGTAAGCCCGACGGCGACGTGCTCGCCA
TGAAGGTGGTCGAGGAGATTCACCGCCGGCTGCCGAACACCCATCTGGTGATGCATGGCTCGTCCTCGGTGCCGCAGGAT
CTGCAGGAAGCCTTCAACAAATACGGCGGCGCGATGCCGCAGACGTTCGGTGTGCCGGTCGAGGAGATCGTGCGCGGCAT
CAAGCACGGCGTCCGCAAGGTCAACATCGACACCGACTGTCGCCTGGCGATGACCGGGGTGTTCCGCAAGATCGCGACCG
AGCACAAGGCCGAGTTCGATCCGCGCAAGTTCCTCAAGCCCGCGATGGACGCGATGCGCGACCTCTGCCGGCTGCGCTTC
GAACAGTTCGGCACCGCCGGCCACGCCTCGAAGATCAAGGTGATCCCGCTGTCGGAAATGGCCAAGCGCTACAAATCCGG
CGCGCTCGATCCGCAGATCGGCCAGGTCGCAAGAGCCGCAGAGTAG

Upstream 100 bases:

>100_bases
CACGGAGGCGGCCAAGGCCGCGATCGCCCGCAGCAAACATTGATCAGCATGACACCGATCAACACCAGATCGACATTCAA
CATTTAGGACAGGAGCCATC

Downstream 100 bases:

>100_bases
TTCGAAACAATCGGGAGCGGCCGCCGGCCGCCCCGCACTCGCCATTCAGGAGACCACGATGGACCAGTCGAGCCGCTACG
CCAACCTCAACCTCAAAGAA

Product: fructose-1,6-bisphosphate aldolase

Products: NA

Alternate protein names: FBP aldolase; FBPA; Fructose-1,6-bisphosphate aldolase; Fructose-bisphosphate aldolase II [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MARITLRQLLDHAAEHGYGVPAFNINNMEQGLAIMEAAAAVDAPVIIQASRGARSYANDIMLAKMIDALAEMYPDIPLCM
HQDHGNDEATCATAIKYGFTSVMMDGSLKADAKTAADYEYNVDITRRVTDMAHWVGASVEGELGVLGSLEHGGGEQEDGH
GVEGKVSREQLLTDPDQAVDFVRATKVDALAIAMGTSHGAYKFSRKPDGDVLAMKVVEEIHRRLPNTHLVMHGSSSVPQD
LQEAFNKYGGAMPQTFGVPVEEIVRGIKHGVRKVNIDTDCRLAMTGVFRKIATEHKAEFDPRKFLKPAMDAMRDLCRLRF
EQFGTAGHASKIKVIPLSEMAKRYKSGALDPQIGQVARAAE

Sequences:

>Translated_361_residues
MARITLRQLLDHAAEHGYGVPAFNINNMEQGLAIMEAAAAVDAPVIIQASRGARSYANDIMLAKMIDALAEMYPDIPLCM
HQDHGNDEATCATAIKYGFTSVMMDGSLKADAKTAADYEYNVDITRRVTDMAHWVGASVEGELGVLGSLEHGGGEQEDGH
GVEGKVSREQLLTDPDQAVDFVRATKVDALAIAMGTSHGAYKFSRKPDGDVLAMKVVEEIHRRLPNTHLVMHGSSSVPQD
LQEAFNKYGGAMPQTFGVPVEEIVRGIKHGVRKVNIDTDCRLAMTGVFRKIATEHKAEFDPRKFLKPAMDAMRDLCRLRF
EQFGTAGHASKIKVIPLSEMAKRYKSGALDPQIGQVARAAE
>Mature_360_residues
ARITLRQLLDHAAEHGYGVPAFNINNMEQGLAIMEAAAAVDAPVIIQASRGARSYANDIMLAKMIDALAEMYPDIPLCMH
QDHGNDEATCATAIKYGFTSVMMDGSLKADAKTAADYEYNVDITRRVTDMAHWVGASVEGELGVLGSLEHGGGEQEDGHG
VEGKVSREQLLTDPDQAVDFVRATKVDALAIAMGTSHGAYKFSRKPDGDVLAMKVVEEIHRRLPNTHLVMHGSSSVPQDL
QEAFNKYGGAMPQTFGVPVEEIVRGIKHGVRKVNIDTDCRLAMTGVFRKIATEHKAEFDPRKFLKPAMDAMRDLCRLRFE
QFGTAGHASKIKVIPLSEMAKRYKSGALDPQIGQVARAAE

Specific function: Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3- phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis [H]

COG id: COG0191

COG function: function code G; Fructose/tagatose bisphosphate aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class II fructose-bisphosphate aldolase family [H]

Homologues:

Organism=Escherichia coli, GI87082041, Length=329, Percent_Identity=32.2188449848024, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1789526, Length=330, Percent_Identity=32.1212121212121, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI1788072, Length=283, Percent_Identity=29.3286219081272, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789293, Length=358, Percent_Identity=24.0223463687151, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006412
- InterPro:   IPR000771 [H]

Pfam domain/function: PF01116 F_bP_aldolase [H]

EC number: =4.1.2.13 [H]

Molecular weight: Translated: 39348; Mature: 39217

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00602 ALDOLASE_CLASS_II_1 ; PS00806 ALDOLASE_CLASS_II_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARITLRQLLDHAAEHGYGVPAFNINNMEQGLAIMEAAAAVDAPVIIQASRGARSYANDI
CCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
MLAKMIDALAEMYPDIPLCMHQDHGNDEATCATAIKYGFTSVMMDGSLKADAKTAADYEY
HHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCEE
NVDITRRVTDMAHWVGASVEGELGVLGSLEHGGGEQEDGHGVEGKVSREQLLTDPDQAVD
CCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHH
FVRATKVDALAIAMGTSHGAYKFSRKPDGDVLAMKVVEEIHRRLPNTHLVMHGSSSVPQD
HHHHHHHHHEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHH
LQEAFNKYGGAMPQTFGVPVEEIVRGIKHGVRKVNIDTDCRLAMTGVFRKIATEHKAEFD
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCCC
PRKFLKPAMDAMRDLCRLRFEQFGTAGHASKIKVIPLSEMAKRYKSGALDPQIGQVARAA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHCCCCCCHHHHHHHCC
E
C
>Mature Secondary Structure 
ARITLRQLLDHAAEHGYGVPAFNINNMEQGLAIMEAAAAVDAPVIIQASRGARSYANDI
CCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
MLAKMIDALAEMYPDIPLCMHQDHGNDEATCATAIKYGFTSVMMDGSLKADAKTAADYEY
HHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCEE
NVDITRRVTDMAHWVGASVEGELGVLGSLEHGGGEQEDGHGVEGKVSREQLLTDPDQAVD
CCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHH
FVRATKVDALAIAMGTSHGAYKFSRKPDGDVLAMKVVEEIHRRLPNTHLVMHGSSSVPQD
HHHHHHHHHEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHH
LQEAFNKYGGAMPQTFGVPVEEIVRGIKHGVRKVNIDTDCRLAMTGVFRKIATEHKAEFD
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCCC
PRKFLKPAMDAMRDLCRLRFEQFGTAGHASKIKVIPLSEMAKRYKSGALDPQIGQVARAA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHCCCCCCHHHHHHHCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1939098 [H]