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The map label for this gene is 86748009

Identifier: 86748009

GI number: 86748009

Start: 1010368

End: 1012026

Strand: Reverse

Name: 86748009

Synonym: RPB_0883

Alternate gene names: NA

Gene position: 1012026-1010368 (Counterclockwise)

Preceding gene: 86748012

Following gene: 86748008

Centisome position: 18.98

GC content: 68.78

Gene sequence:

>1659_bases
ATGACGACAGATGCGGACGTGATCGTGATCGGCGCCGGCCTCGCCGGTCTGGTCGCCGCCACCGAGATCGCGGACGCCGG
ACGCTCGGTGATCGTGATCGATCAGGAGGGCGAACAGAATTGCGGCGGCCAGGCGTTCTGGTCGTTCGGCGGCCTGTTCC
TGGTCGACTCGCCGGAGCAACGCCGGCTTGGAATTCGCGACAGCTACGAACTCGCGCTGCAGGACTGGCTCGGCGCGGCC
GGTTTCGACCGCGACGAGGATCTGTGGCCGCGGCGCTGGGCCGAAGCCTATCTCGGCTTCGCCGCCGGCGAGAAGCGCGA
CTGGCTGCGCGCAATGGGGCACAGGATCTTTCCGGTGGTCGGCTGGGCCGAGCGCGGCGGCTACGACGCCATGAGCCACG
GCAATTCGGTGCCGCGCTTCCATGTCACCTGGGGCACCGGGCCCGGGCTGATCGAACCGTTCGTGCGCCGCGCCCGCGCC
GCACAGGATTCCGGCCGGCTCGGCTTCCGGTTCCGCCACCGCGTCGATGCGCTGACGCTGACCAACGGCGTCGTCGAGGG
CGTCAGCGGCGCGGTGCTCGAGCCGACGCAGGCGCCGCGCGGCGCAAGTAGTTCGCGCAAGGTGATCGGTGACTTCACGC
TGCGCGCCCAGGCGGTGATCGTCGCCTCCGGCGGCATCGGCGGCAATCACGAGCTGGTGCGACAGAATTGGCCGCAGCGG
CTGGGCAAGCCGCCGCAACGGATGATCTCCGGCGTGCCCGCGCATGTCGACGGCCGGATGCTCGGCATCACCGAGGCCGC
CGGCGCGCGGCTGATCAACCGCGACCGGATGTGGCACTATGTCGAGGGCCTGCAGAATTTCGATCCGATCTGGCCGCGCC
ACGGCATCCGGATTTTACCCGGACCGTCGTCGATGTGGTTCGACGCCACCGGCAAACGGCTGCCGGCGCCGCTGTTTCCC
GGCTCCGACACGCTCGGCCAGCTCCACGCCATCATGGCGACCGGCTACGACTATTCCTGGTTCGTGCTGACGCAGGCGAT
CATCAAGAAGGAATTCGCGCTGTCGGGTTCCGAGCAGAATCCGGACCTGACCGGCAAGAGCTGGCTGATGACCGCGCGCC
GCGCCACCAACAAGGCCGCGCCGGCGCCGGTCGAAGCGTTCAAGCAGCACGGCGCCGACTTCATCGTCCGCGACAATCTC
GACGATCTGGTCGTCGCGATGAACGCGCTCAGCGGCGACAATCTGCTGAAGCCGGGCGAACTGCGCAGCCAGATCGAGGC
GCGCGACCGCGAGATCGACAATGCCTTCGTCAAGGACGCGCAGGTGATGAACATCCACAATGCGCGGCGCTATATCGGCG
ACCGGCTGATCCGCACCGCGAAGCCGCACAAGATTCTCGATCCCGCCTACGGCCCGCTGATCGCGGTGCGGCTCAACATC
CTCACCCGCAAGACGCTCGGCGGATTCGAGACCGATCTCGACGCGCGGGTGTTCGGCCAGGACGGCGCGATCATTCCGGG
GCTGTACGCGGTCGGCGAAGCCGCCGGCTTCGGCGGCGGCGGCATGCACGGCTATCGCTCGCTCGAAGGCACCTTCCTCG
GCGGCTGCCTGTTCTCCGGCCGCAACGCCGGCCGCGCGGCGGCGAAAGCGGTGGCGTGA

Upstream 100 bases:

>100_bases
GGCACGAGTCCGGGTCCCAATCTGAAACAGCATGGACACGAAAGCCGTTGACCGCGCCGGGCCGCGCGCGTTTCAATCGG
TTCCTCCACAGGGAACGCCC

Downstream 100 bases:

>100_bases
CAGGCGAATTAGTCCTCAACGGCGCGCGATGCGGACGCCGGCTGACAGAGTACTAACAGCAGCGCTCAGCTTGCGCGGCG
CGCCGGCAAGCCGTCGTTAA

Product: putative FAD-binding dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 552; Mature: 551

Protein sequence:

>552_residues
MTTDADVIVIGAGLAGLVAATEIADAGRSVIVIDQEGEQNCGGQAFWSFGGLFLVDSPEQRRLGIRDSYELALQDWLGAA
GFDRDEDLWPRRWAEAYLGFAAGEKRDWLRAMGHRIFPVVGWAERGGYDAMSHGNSVPRFHVTWGTGPGLIEPFVRRARA
AQDSGRLGFRFRHRVDALTLTNGVVEGVSGAVLEPTQAPRGASSSRKVIGDFTLRAQAVIVASGGIGGNHELVRQNWPQR
LGKPPQRMISGVPAHVDGRMLGITEAAGARLINRDRMWHYVEGLQNFDPIWPRHGIRILPGPSSMWFDATGKRLPAPLFP
GSDTLGQLHAIMATGYDYSWFVLTQAIIKKEFALSGSEQNPDLTGKSWLMTARRATNKAAPAPVEAFKQHGADFIVRDNL
DDLVVAMNALSGDNLLKPGELRSQIEARDREIDNAFVKDAQVMNIHNARRYIGDRLIRTAKPHKILDPAYGPLIAVRLNI
LTRKTLGGFETDLDARVFGQDGAIIPGLYAVGEAAGFGGGGMHGYRSLEGTFLGGCLFSGRNAGRAAAKAVA

Sequences:

>Translated_552_residues
MTTDADVIVIGAGLAGLVAATEIADAGRSVIVIDQEGEQNCGGQAFWSFGGLFLVDSPEQRRLGIRDSYELALQDWLGAA
GFDRDEDLWPRRWAEAYLGFAAGEKRDWLRAMGHRIFPVVGWAERGGYDAMSHGNSVPRFHVTWGTGPGLIEPFVRRARA
AQDSGRLGFRFRHRVDALTLTNGVVEGVSGAVLEPTQAPRGASSSRKVIGDFTLRAQAVIVASGGIGGNHELVRQNWPQR
LGKPPQRMISGVPAHVDGRMLGITEAAGARLINRDRMWHYVEGLQNFDPIWPRHGIRILPGPSSMWFDATGKRLPAPLFP
GSDTLGQLHAIMATGYDYSWFVLTQAIIKKEFALSGSEQNPDLTGKSWLMTARRATNKAAPAPVEAFKQHGADFIVRDNL
DDLVVAMNALSGDNLLKPGELRSQIEARDREIDNAFVKDAQVMNIHNARRYIGDRLIRTAKPHKILDPAYGPLIAVRLNI
LTRKTLGGFETDLDARVFGQDGAIIPGLYAVGEAAGFGGGGMHGYRSLEGTFLGGCLFSGRNAGRAAAKAVA
>Mature_551_residues
TTDADVIVIGAGLAGLVAATEIADAGRSVIVIDQEGEQNCGGQAFWSFGGLFLVDSPEQRRLGIRDSYELALQDWLGAAG
FDRDEDLWPRRWAEAYLGFAAGEKRDWLRAMGHRIFPVVGWAERGGYDAMSHGNSVPRFHVTWGTGPGLIEPFVRRARAA
QDSGRLGFRFRHRVDALTLTNGVVEGVSGAVLEPTQAPRGASSSRKVIGDFTLRAQAVIVASGGIGGNHELVRQNWPQRL
GKPPQRMISGVPAHVDGRMLGITEAAGARLINRDRMWHYVEGLQNFDPIWPRHGIRILPGPSSMWFDATGKRLPAPLFPG
SDTLGQLHAIMATGYDYSWFVLTQAIIKKEFALSGSEQNPDLTGKSWLMTARRATNKAAPAPVEAFKQHGADFIVRDNLD
DLVVAMNALSGDNLLKPGELRSQIEARDREIDNAFVKDAQVMNIHNARRYIGDRLIRTAKPHKILDPAYGPLIAVRLNIL
TRKTLGGFETDLDARVFGQDGAIIPGLYAVGEAAGFGGGGMHGYRSLEGTFLGGCLFSGRNAGRAAAKAVA

Specific function: Able to catalyze the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3-ketosteroids, but the ketosteroid dehydrogenase activity is low compared to KsdD in the cholesterol degradation process. The low activ

COG id: COG3573

COG function: function code R; Predicted oxidoreductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family [H]

Homologues:

Organism=Caenorhabditis elegans, GI71986328, Length=552, Percent_Identity=24.4565217391304, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 59891; Mature: 59760

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTDADVIVIGAGLAGLVAATEIADAGRSVIVIDQEGEQNCGGQAFWSFGGLFLVDSPEQ
CCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHCCCEEEEECCHH
RRLGIRDSYELALQDWLGAAGFDRDEDLWPRRWAEAYLGFAAGEKRDWLRAMGHRIFPVV
HCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEE
GWAERGGYDAMSHGNSVPRFHVTWGTGPGLIEPFVRRARAAQDSGRLGFRFRHRVDALTL
EEHHCCCCCHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEHHHHCEEEH
TNGVVEGVSGAVLEPTQAPRGASSSRKVIGDFTLRAQAVIVASGGIGGNHELVRQNWPQR
HHHHHHCCCCCEECCCCCCCCCCCCCEEEEEEEEEEEEEEEEECCCCCCHHHHHHHCHHH
LGKPPQRMISGVPAHVDGRMLGITEAAGARLINRDRMWHYVEGLQNFDPIWPRHGIRILP
HCCCHHHHHCCCCCCCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEC
GPSSMWFDATGKRLPAPLFPGSDTLGQLHAIMATGYDYSWFVLTQAIIKKEFALSGSEQN
CCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCC
PDLTGKSWLMTARRATNKAAPAPVEAFKQHGADFIVRDNLDDLVVAMNALSGDNLLKPGE
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCCCHH
LRSQIEARDREIDNAFVKDAQVMNIHNARRYIGDRLIRTAKPHKILDPAYGPLIAVRLNI
HHHHHHHHHHHHHHHHHCCHHHEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHH
LTRKTLGGFETDLDARVFGQDGAIIPGLYAVGEAAGFGGGGMHGYRSLEGTFLGGCLFSG
HHHHHCCCCCCCCCCEEECCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECC
RNAGRAAAKAVA
CCCCHHHHHHCC
>Mature Secondary Structure 
TTDADVIVIGAGLAGLVAATEIADAGRSVIVIDQEGEQNCGGQAFWSFGGLFLVDSPEQ
CCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHCCCEEEEECCHH
RRLGIRDSYELALQDWLGAAGFDRDEDLWPRRWAEAYLGFAAGEKRDWLRAMGHRIFPVV
HCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEE
GWAERGGYDAMSHGNSVPRFHVTWGTGPGLIEPFVRRARAAQDSGRLGFRFRHRVDALTL
EEHHCCCCCHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEHHHHCEEEH
TNGVVEGVSGAVLEPTQAPRGASSSRKVIGDFTLRAQAVIVASGGIGGNHELVRQNWPQR
HHHHHHCCCCCEECCCCCCCCCCCCCEEEEEEEEEEEEEEEEECCCCCCHHHHHHHCHHH
LGKPPQRMISGVPAHVDGRMLGITEAAGARLINRDRMWHYVEGLQNFDPIWPRHGIRILP
HCCCHHHHHCCCCCCCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEC
GPSSMWFDATGKRLPAPLFPGSDTLGQLHAIMATGYDYSWFVLTQAIIKKEFALSGSEQN
CCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCC
PDLTGKSWLMTARRATNKAAPAPVEAFKQHGADFIVRDNLDDLVVAMNALSGDNLLKPGE
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCCCHH
LRSQIEARDREIDNAFVKDAQVMNIHNARRYIGDRLIRTAKPHKILDPAYGPLIAVRLNI
HHHHHHHHHHHHHHHHHCCHHHEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHH
LTRKTLGGFETDLDARVFGQDGAIIPGLYAVGEAAGFGGGGMHGYRSLEGTFLGGCLFSG
HHHHHCCCCCCCCCCEEECCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECC
RNAGRAAAKAVA
CCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA