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The map label for this gene is pyrE

Identifier: 86747973

GI number: 86747973

Start: 964389

End: 964952

Strand: Reverse

Name: pyrE

Synonym: RPB_0847

Alternate gene names: 86747973

Gene position: 964952-964389 (Counterclockwise)

Preceding gene: 86747974

Following gene: 86747972

Centisome position: 18.1

GC content: 67.2

Gene sequence:

>564_bases
GTGTCCAAATCCGCGTCCCGCGCCCGCCTTGCCGACATCATCCGCACCCGCTCGTTCGGCCGTGGCGAGATCACGCTGGC
GTCGGGGCGCAAGAGCGACTTCTATTTCAATCTGAAGCCGACGATGCTCGACCCCGAGGGCGCGGCGCTGCTGGCGGAAT
TGACCTATGAGGCGCTGCGCGACGAGCAGGTCGACTATATCGGCGGGCTGGAGATGGGCGCGGTGCCGCTGGCCGGCGCG
ATCGCGCAGCTGTCGTGGCTGAAAAACCACCCGATCGCGGCGTTTTTTGTGCGCAAGAAGCCGAAGGAGCACGGCGCCCG
GCTGGCGGTCGAAGGTCTGGCGAAGGGCGAGAGCCTCGCCGGCAAGCGCTGCGTCATCGTCGAGGATGTCACCACCACCG
GCGGCTCGGCCATCAAGGCGGTCGAGGCGGTGCGCGAATCCGGTGCCGAGATCGTGCTGGTGCTGACCATGGTCGACCGC
GAGGAAGGCGCCACCGAAGCCTTCGCCGCAGCCGGGCTGCCGTTCCGATCGCTGTACAAGGCCAGCGAATTCCTCAATCG
CTGA

Upstream 100 bases:

>100_bases
GCGACGACGCAATCCCAAATCGTGAATACCGCCGCTGCCCGCTGGCATCGGCCGATGCCGGCGGCTAGATTGCGGCCGGT
CAACCTCAGAGAGCCCGCCC

Downstream 100 bases:

>100_bases
ACACCCCGGCGCCCCGAAGAAACCGCGCGTTTACCATCCCGCACTAAAGTGAACGGGTCGCGGCCAGGCCGCAGCACGAT
CGCGTCGGGTGGAGTGAGCG

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 187; Mature: 186

Protein sequence:

>187_residues
MSKSASRARLADIIRTRSFGRGEITLASGRKSDFYFNLKPTMLDPEGAALLAELTYEALRDEQVDYIGGLEMGAVPLAGA
IAQLSWLKNHPIAAFFVRKKPKEHGARLAVEGLAKGESLAGKRCVIVEDVTTTGGSAIKAVEAVRESGAEIVLVLTMVDR
EEGATEAFAAAGLPFRSLYKASEFLNR

Sequences:

>Translated_187_residues
MSKSASRARLADIIRTRSFGRGEITLASGRKSDFYFNLKPTMLDPEGAALLAELTYEALRDEQVDYIGGLEMGAVPLAGA
IAQLSWLKNHPIAAFFVRKKPKEHGARLAVEGLAKGESLAGKRCVIVEDVTTTGGSAIKAVEAVRESGAEIVLVLTMVDR
EEGATEAFAAAGLPFRSLYKASEFLNR
>Mature_186_residues
SKSASRARLADIIRTRSFGRGEITLASGRKSDFYFNLKPTMLDPEGAALLAELTYEALRDEQVDYIGGLEMGAVPLAGAI
AQLSWLKNHPIAAFFVRKKPKEHGARLAVEGLAKGESLAGKRCVIVEDVTTTGGSAIKAVEAVRESGAEIVLVLTMVDRE
EGATEAFAAAGLPFRSLYKASEFLNR

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=172, Percent_Identity=26.7441860465116, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17554964, Length=181, Percent_Identity=29.2817679558011, Blast_Score=73, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17508631, Length=177, Percent_Identity=27.683615819209, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17933654, Length=182, Percent_Identity=29.1208791208791, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_RHOP2 (Q2J1V2)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_484469.1
- ProteinModelPortal:   Q2J1V2
- SMR:   Q2J1V2
- STRING:   Q2J1V2
- GeneID:   3909105
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0847
- eggNOG:   COG0461
- HOGENOM:   HBG404341
- OMA:   GKEADYY
- ProtClustDB:   PRK00455
- BioCyc:   RPAL316058:RPB_0847-MONOMER
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 20101; Mature: 19970

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 98-98 BINDING 99-99 BINDING 102-102 BINDING 104-104 BINDING 132-132 BINDING 160-160

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSASRARLADIIRTRSFGRGEITLASGRKSDFYFNLKPTMLDPEGAALLAELTYEALR
CCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEECCCCEECCCCHHHHHHHHHHHHC
DEQVDYIGGLEMGAVPLAGAIAQLSWLKNHPIAAFFVRKKPKEHGARLAVEGLAKGESLA
CCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHCCEEEEHHHHCCCCCC
GKRCVIVEDVTTTGGSAIKAVEAVRESGAEIVLVLTMVDREEGATEAFAAAGLPFRSLYK
CCEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHCCCCHHHHHH
ASEFLNR
HHHHHCC
>Mature Secondary Structure 
SKSASRARLADIIRTRSFGRGEITLASGRKSDFYFNLKPTMLDPEGAALLAELTYEALR
CCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEECCCCEECCCCHHHHHHHHHHHHC
DEQVDYIGGLEMGAVPLAGAIAQLSWLKNHPIAAFFVRKKPKEHGARLAVEGLAKGESLA
CCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHCCEEEEHHHHCCCCCC
GKRCVIVEDVTTTGGSAIKAVEAVRESGAEIVLVLTMVDREEGATEAFAAAGLPFRSLYK
CCEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHCCCCHHHHHH
ASEFLNR
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA