Click here to switch to the map view.

The map label for this gene is nuoE [H]

Identifier: 86747852

GI number: 86747852

Start: 816596

End: 817066

Strand: Reverse

Name: nuoE [H]

Synonym: RPB_0726

Alternate gene names: 86747852

Gene position: 817066-816596 (Counterclockwise)

Preceding gene: 86747853

Following gene: 86747851

Centisome position: 15.32

GC content: 72.19

Gene sequence:

>471_bases
ATGCAGAATGAACCATGGGATCCGACCCGCGCCGCTGAGATCATCGCCGGGTTGACGCACAAGGAAGGGCCGACGCTGGT
GATCCTGCACGCCTTGCAGGAGGCGTTCGGCCATGTGCCGGCGGCCGCCGAGCCGATGGTGGCGCAGGCGCTCAACCTGT
CGCGCGCCGAAGTGCACGGCGTCCTCACCTTCTATCCGGATTTCCGCCGCACCCCGCCGGGCCGCCACGTGCTCAAGCTG
TGCCGCGCCGAGGCCTGCCAGGCGGCCGGCGGCGACGCGCTCGCCGCGCGCGCCGAAACCCGGCTCGGCGTGACCTTCGG
CGCCACCACGGCCGACGGCGCGGTGACGCTGGAGCCGACCTATTGCCTCGGCCTGTGCGCCACGGCGCCGGCGGCGATGC
TGAACGAGCGCGTGATCGGCCGGCTCGATGCCGGCAAGCTCGATGCGCTGCTGACGGAGGCGCAGCCATGA

Upstream 100 bases:

>100_bases
CGCTTCATTATCGTTTCCGTATCGCACCATTGGACAGCTTTATTGATTCTTTGAACAATTCTTATTCTCATCGGGCGAGC
CACGACGACGAAGAGCGCCG

Downstream 100 bases:

>100_bases
GCCTGCGCGTCTTCATCCCGCGCGACTCCGGCGCGCTTGCGGTCGGCGCCGACGAGGCCGCGGCCGAACTCACCCGCGTC
GCCCATGACCGCCACCTGCC

Product: formate dehydrogenase subunit gamma

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E [H]

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MQNEPWDPTRAAEIIAGLTHKEGPTLVILHALQEAFGHVPAAAEPMVAQALNLSRAEVHGVLTFYPDFRRTPPGRHVLKL
CRAEACQAAGGDALAARAETRLGVTFGATTADGAVTLEPTYCLGLCATAPAAMLNERVIGRLDAGKLDALLTEAQP

Sequences:

>Translated_156_residues
MQNEPWDPTRAAEIIAGLTHKEGPTLVILHALQEAFGHVPAAAEPMVAQALNLSRAEVHGVLTFYPDFRRTPPGRHVLKL
CRAEACQAAGGDALAARAETRLGVTFGATTADGAVTLEPTYCLGLCATAPAAMLNERVIGRLDAGKLDALLTEAQP
>Mature_156_residues
MQNEPWDPTRAAEIIAGLTHKEGPTLVILHALQEAFGHVPAAAEPMVAQALNLSRAEVHGVLTFYPDFRRTPPGRHVLKL
CRAEACQAAGGDALAARAETRLGVTFGATTADGAVTLEPTYCLGLCATAPAAMLNERVIGRLDAGKLDALLTEAQP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=136, Percent_Identity=29.4117647058824, Blast_Score=77, Evalue=6e-15,
Organism=Escherichia coli, GI1788621, Length=137, Percent_Identity=35.7664233576642, Blast_Score=84, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17561328, Length=136, Percent_Identity=31.6176470588235, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI18859877, Length=136, Percent_Identity=30.1470588235294, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355353, Length=132, Percent_Identity=28.7878787878788, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 16419; Mature: 16419

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNEPWDPTRAAEIIAGLTHKEGPTLVILHALQEAFGHVPAAAEPMVAQALNLSRAEVHG
CCCCCCCHHHHHHHHHHCCCCCCCCEEHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHE
VLTFYPDFRRTPPGRHVLKLCRAEACQAAGGDALAARAETRLGVTFGATTADGAVTLEPT
EEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEEEECCCCCCCEEEECCH
YCLGLCATAPAAMLNERVIGRLDAGKLDALLTEAQP
HHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MQNEPWDPTRAAEIIAGLTHKEGPTLVILHALQEAFGHVPAAAEPMVAQALNLSRAEVHG
CCCCCCCHHHHHHHHHHCCCCCCCCEEHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHE
VLTFYPDFRRTPPGRHVLKLCRAEACQAAGGDALAARAETRLGVTFGATTADGAVTLEPT
EEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEEEECCCCCCCEEEECCH
YCLGLCATAPAAMLNERVIGRLDAGKLDALLTEAQP
HHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]